Abstract:
:rho(1) GABA(C) receptor antagonists inhibit myopia in chick but the site of this effect is not known. The sclera ultimately determines the shape and size of the globe and thus an untested possibility is that GABA agents have a scleral mechanism. Whether rho(1) GABA(C) receptors are expressed and located in chick sclera is unknown. Real-time PCR, western blot and immunohistochemistry were used to determine whether rho1 GABA(C) receptors are expressed and located in chick fibrous and cartilaginous sclera. Both layers of the chick sclera were positive for rho1 GABA(C) receptor mRNA (PCR) and protein (western blot) expression and labeling was observed in both fibroblasts and chondrocytes of the fibrous and cartilaginous layers (immunohistochemistry). These investigations clearly show that chick sclera possesses rho(1) GABA(C) receptors. The sclera is thus a potential previously unrecognized site for activity of rho(1) GABA(C) agents.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Cheng ZY,Chebib M,Schmid KLdoi
10.1111/j.1471-4159.2011.07300.xsubject
Has Abstractpub_date
2011-07-01 00:00:00pages
281-7issue
2eissn
0022-3042issn
1471-4159journal_volume
118pub_type
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