Abstract:
:Transitional cell carcinomas (TCCs) of the urinary bladder are common malignancies with a high recurrence rate. Since microRNA-21 (miR-21) may contribute to tumorigenesis and chemoresistance in many cancer types, we aimed to investigate its efficacy in TCCs. The expression of miR-21 and its target PTEN was determined by real-time qRT-PCR and western blotting, respectively in tumor tissues as well as adjacent non-tumor mucosa. The effect of miR-21 on cell proliferation and chemosensitivity to doxorubicin were measured using the MTT method. Cell apoptosis induced by doxorubicin was investigated using flow cytometry in the T24 cell line. BCL-2, AKT and pAKT were detected by western blotting for analysis of potential mechanisms. miR-21 was significantly up-regulated in tumor tissues while PTEN was expressed in lower levels compared to non-tumor tissues. A negative correlation between expression of miR-21 and PTEN was established in vivo. Cell proliferation and chemoresistance to doxorubicin were promoted by overexpression of miR-21 in T24 cells. BCL-2 up-regulation could be achieved by miR-21 overexpression, which prevented T24 cells from apoptosis induced by doxorubicin. Furthermore, the miR-21 induced BCL-2 up-regulation could be cancelled by the PI3K inhibitor LY294002. These data verified the oncogenic role of miR-21 in TCCs and may usher in new therapeutic strategies in treating this disease.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Tao J,Lu Q,Wu D,Li P,Xu B,Qing W,Wang M,Zhang Z,Zhang Wdoi
10.3892/or.2011.1245subject
Has Abstractpub_date
2011-06-01 00:00:00pages
1721-9issue
6eissn
1021-335Xissn
1791-2431journal_volume
25pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::It has been shown that overexpression of signal transducer and activator of transcription 3 (Stat3) contribute to the progression and metastasis of various solid tumors and that silencing Stat3 inhibits tumor growth in several types of cancer. Gene associated with retinoid-IFN-induced mortality 19 (GRIM-19), a Stat3-i...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3233
更新日期:2014-08-01 00:00:00
abstract::Flavonoids exhibit chemopreventive and chemotherapeutic effects. Butein, a bioactive flavonoid isolated from numerous native plants, has been shown to induce apoptosis in human cancer cells. In the current study, the molecular mechanisms of butein action on cell proliferation and apoptosis of neuroblastoma cells were ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1632
更新日期:2012-04-01 00:00:00
abstract::Dysregulation of microRNAs (miRNAs) occurs frequently in cervical carcinogenesis. miRNAs function as tumor-suppressors or oncogenes and are involved in tumor behavior. However, the expression and function of miR-27b in cervical carcinogenesis remain unknown. In the present study, we observed that miR-27b was significa...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4500
更新日期:2016-03-01 00:00:00
abstract::New therapeutic strategies are needed for colorectal cancer (CRC) treatment. MicroRNAs are involved in cancer‑pertinent cellular processes, including chemoresistance. As miR‑302a is an embryonic stem cell‑specific microRNA, studies on miR‑302a have focused on its role in human stem cells. Studies analyzing miR‑302 fun...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.5237
更新日期:2017-01-01 00:00:00
abstract::MAP kinase signaling proteins have major implications in the molecular oncogenesis of breast cancers and have been extensively investigated as putative targets for therapy. This study reports the investigation of the expression of P38 MAPK and its phosphorylated form (p-P38 MAPK) in clinical specimens of invasive brea...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2645
更新日期:2013-10-01 00:00:00
abstract::The lipogenic enzyme fatty acid synthase (FAS) is differentially overexpressed and hyperactivated in a biologically aggressive subset of breast carcinomas and minimally in most normal adult tissues, rendering it an interesting target for anti-neoplastic therapy development. Current trends in the treatment of human bre...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-08-01 00:00:00
abstract::A growing number of studies suggest that the hepatitis B virus X protein (HBx) enhances the protein stability of the hypoxia-inducible factor-1α (HIF-1α). However, the relationship between hepatitis B virus (HBV), HBx and hypoxia-inducible factor-2α (HIF-2α) has not yet been fully elucidated. Immunohistochemical analy...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4480
更新日期:2016-03-01 00:00:00
abstract::The overexpression of EGFR and/or HER-2 is associated with tumor cell resistance to chemotherapy, radiotherapy, disease progression and poor prognosis in patients with a variety of malignant tumors. Treatment combining the EGFR-targeting drug, gefitinib (ZD1839, Iressa) with the HER-2-targeting drug, trastuzumab (Herc...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-08-01 00:00:00
abstract::The interface between malignant melanoma and patient immunity has long been recognised and efforts to treat this most lethal form of skin cancer by activating immune responses with cytokine, vaccine and also antibody immunotherapies have demonstrated promise in limited subsets of patients. In the present study, we dis...
journal_title:Oncology reports
pub_type: 杂志文章,评审
doi:10.3892/or.2014.3275
更新日期:2014-09-01 00:00:00
abstract::The present study aimed to assess the in vitro and in vivo magnetic resonance imaging (MRI) features of chlorotoxin (CTX)-conjugated superparamagnetic iron oxide (SPIO) nanoprobes. CTX-conjugated nanoprobes were composed of SPIO coated with polyethylene glycol (PEG) and conjugated with CTX. The nanoprobes were termed ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4629
更新日期:2016-05-01 00:00:00
abstract::Histone deacetylase (HDAC) 4 is an emerging target in cancer therapeutics, but little is known about the function of HDAC4 in gynecologic malignancies. Therefore we investigated the mechanism of HDAC4 promoting the proliferation of epithelial ovarian cancer cells (OV). In this study, we observed that the proliferation...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4423
更新日期:2016-02-01 00:00:00
abstract::The present study determined the anticancer activity and its mechanism of microRNA‑133b on cell proliferation of cisplatin-induced non-small cell lung cancer cells. The expression of microRNA-133b cisplatin‑induced non-small cell lung cancer (NSCLC) tissue was lower than that of para-carcinoma tissue in patients. Over...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6215
更新日期:2018-03-01 00:00:00
abstract::Nucleostemin (NS) plays an important role in tumorigenesis and progression. Most studies consider that NS plays its role through combining with p53 and inhibiting it, however our previous studies revealed that NS could also function without the existence of p53. To date, few studies have focused on the p53-independent...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3240
更新日期:2014-08-01 00:00:00
abstract::The aim of the present study was to investigate the role of miR‑203a‑3p in colorectal cancer (CRC) and identify the target gene of microRNA (miR)‑203a‑3p. A total of 59 sets of cancer tissues and corresponding adjacent non‑tumor tissues were collected from CRC patients (aged 31‑78 years) between October 2016 and May 2...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7310
更新日期:2019-11-01 00:00:00
abstract::Prostate cancer (PCa) remains a leading cause of mortality among men in the United States and Western Europe. The molecular mechanism of PCa pathogenesis has not been fully elucidated. In the present study, the expression profile of E2F transcription factor 7 (E2F7) in PCa was examined using immunohistochemistry and r...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7659
更新日期:2020-09-01 00:00:00
abstract::Following the publication of the above paper, an interested reader drew to our attention apparent anomalies associated with Figs. 2, 3 and 4; essentially, these three figures contained panels exhibiting overlapping data, such that data purportedly relating to different experiments were apparently drawn from the same o...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7868
更新日期:2020-11-25 00:00:00
abstract::Gelatinases play an important role in tumor growth and metastasis, and overexpression of these molecules is strongly correlated with poor prognosis in a variety of malignant tumors. Lidamycin is an enediyne antitumor antibiotic with potent cytotoxicity. We previously reported that a...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1910
更新日期:2012-10-01 00:00:00
abstract::Histone deacetylase inhibitors (HDIs) as well as non-steroidal anti-inflammatory drugs including aspirin show promise as antineoplastic agents. The treatment with both HDIs and aspirin can result in hyperacetylation of proteins. In this study, we investigated whether HDIs and aspirin interacted in inducing anticancer ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-07-01 00:00:00
abstract::Gene therapy mediated by murine leukemia virus (MLV)-based replicating retrovirus vector (RRV) was previously proven to be highly effective in tumor cell killing, resulting in significant suppression of tumor growth in vivo. Recently, we developed a different form of RRV which is derived from another retrovirus, gibbo...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1789
更新日期:2012-07-01 00:00:00
abstract::Burkitt's lymphoma (BL) is a fast growing cancer of the human lymphatic system, and an extremely invasive B-cell non-Hodgkin's lymphoma. We explored the mechanism of apoptosis in Raji cells associated with the post-transcriptional regulation factors. To confirm that the predicted microRNA-520a (miR-520a) is matched wi...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4975
更新日期:2016-09-01 00:00:00
abstract::Synthetic oligodeoxynucleotides (ODNs) with the CpG-motifs are recognized by toll-like receptor 9 (TLR9), which elicits an immune response. Serum starvation of Raw264.7 cells increased tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) expression. However, treatment wit...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1579
更新日期:2012-04-01 00:00:00
abstract::The objective of this study was to examine how splenectomy affects the immune response, particularly T cells, in chemically-induced mammary tumors. Female rats were splenectomized and then exposed to 9,10-dimethyl-1,2-benz(a)anthracene (DMBA) to induce mammary tumors. Splenectomy significantly decreased the rate of tu...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.9.1.185
更新日期:2002-01-01 00:00:00
abstract::The peroxisome proliferator-activated receptor-γ (PPAR-γ) coactivator-1β (PGC-1β) is a well-established regulator of mitochondrial biogenesis. However, the underlying mechanism of PGC-1β action remains elusive. This study reveals that knockdown of endogenous PGC-1β by short-hairpin RNA (shRNA) leads to a decrease in t...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2628
更新日期:2013-10-01 00:00:00
abstract::The advent of pathotropic (disease-seeking) targeting has transported genetic medicine across the threshold of history with the progressive clinical validation of Rexin-G, a tumor-targeted nanosized anti-cancer agent. Achieving noteworthy single-agent efficacy and survival benefits in otherwise intractable cancers, th...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2010.829
更新日期:2010-10-01 00:00:00
abstract::In this work we studied the effect of v-raf and different form RAF on up regulation of VEGF gene expression and angiogenesis. In the experiments the truncated forms of RAF1 gene were transfected into the cells of the established human urothelial line HCV-29, otherwise non-angiogenic. The plasmids coding for mitochondr...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::The present study was performed to investigate the protective effects of tannic acid (TA) on liver injury induced by arsenic trioxide (ATO) and to elucidate the mechanism involved as related to the Kelch‑like ECH‑associated protein 1 (Keap1)‑nuclear factor erythroid 2‑related factor 2 (Nrf2)/antioxidant response eleme...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7764
更新日期:2020-11-01 00:00:00
abstract::Interleukin‑9 (IL‑9) can function as both a positive and negative regulator of immune response, however the role of IL‑9 in tumor immunity is poorly understood. Chronic lymphocytic leukemia (CLL) is the most common chronic lympho‑proliferative disorder. Twenty CLL patients from 2010 to 2011 were recruited in the study...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6367
更新日期:2018-06-01 00:00:00
abstract::To investigate the antitumor action of arsenic trioxide (As2O3) by intratumoral injection into solid tumors, tumor growth inhibition (TGI) and angiogenesis of heterotransplanted esophageal carcinoma in mice was carried out. The cultured human esophageal carcinoma cells were inoculated into both laterals of the abdomin...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-11-01 00:00:00
abstract::The Nemo‑like kinase (NLK), a conserved serine/threonine kinase, plays a critical role in the regulation of a variety of transcription factors, with important roles in determining cell fate. Although recent studies have demonstrated decreased expression patterns of NLK in various types of human cancer, the functional ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7557
更新日期:2020-06-01 00:00:00
abstract::Breast cancer is the most common malignancy in women, and metastasis is the leading cause of death in breast cancer patients. Previous studies have shown that epithelial‑mesenchymal transition (EMT) is involved in the metastasis of breast cancer, but the metabolic reprogramming and regulation mechanisms involved in th...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7661
更新日期:2020-09-01 00:00:00