Abstract：
:The rodent malaria parasite Plasmodium chabaudi chabaudi shares many features with human malaria species, including P. falciparum, and is the in vivo model of choice for many aspects of malaria research in the mammalian host, from sequestration of parasitized erythrocytes, to antigenic variation and host immunity and immunopathology. This protocol describes an optimized method for the transformation of mature blood-stage P.c. chabaudi and a description of a vector that targets efficient, single crossover integration into the P.c. chabaudi genome. Transformed lines are reproducibly generated and selected within 14-20 d, and show stable long-term protein expression even in the absence of drug selection. This protocol, therefore, provides the scientific community with a robust and reproducible method to generate transformed P.c. chabaudi parasites expressing fluorescent, bioluminescent and model antigens that can be used in vivo to dissect many of the fundamental principles of malaria infection.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Spence PJ,Cunningham D,Jarra W,Lawton J,Langhorne J,Thompson Jdoi
10.1038/nprot.2011.313subject
Has Abstractpub_date
2011-04-01 00:00:00pages
553-561issue
4eissn
1754-2189issn
1750-2799journal_volume
6pub_type
杂志文章相关文献
Nature Protocols文献大全abstract：:Protein ADP-ribosylation is a structurally heterogeneous post-translational modification (PTM) that influences the physicochemical and biological properties of the modified protein. ADP-ribosylation of chromatin changes its structural properties, thereby regulating important nuclear functions. A lack of suitable antib...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2017.072
更新日期：2017-09-01 00:00:00
abstract：:Although differences in protein staining intensity can often be visualized by difference gel electrophoresis, abundant proteins can obscure less abundant proteins, and quantification of post-translational modifications is difficult. We present a protocol for quantifying changes in the abundance of a specific protein o...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2006.7
更新日期：2006-01-01 00:00:00
abstract：:This protocol describes a method for direct labeling and detection of small RNAs present in total RNA by splinted ligation. The assay uses a small RNA-specific bridge oligonucleotide to form base pairs with the small RNA and a 5'-end-radiolabeled ligation oligonucleotide. The captured small RNA is directly labeled by ...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2007.530
更新日期：2008-01-01 00:00:00
abstract：:A key challenge of modern biology is to uncover the functional role of the protein entities that compose cellular proteomes. To this end, the availability of reliable three-dimensional atomic models of proteins is often crucial. This protocol presents a community-wide web-based method using RaptorX (http://raptorx.uch...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2012.085
更新日期：2012-07-19 00:00:00
abstract：:Neurons in cortical sensory regions receive modality-specific information through synapses that are located on their dendrites. Recently, the use of two-photon microscopy combined with whole-cell recordings has helped to identify visually evoked dendritic calcium signals in mouse visual cortical neurons in vivo. The c...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2010.169
更新日期：2011-01-01 00:00:00
abstract：:Although lung transplant is a life-saving therapy for some patients, primary graft dysfunction (PGD) is a leading cause of mortality and morbidity soon after a transplant. Ischemia reperfusion injury is known to be one of the most critical factors in PGD development. PGD is by definition an acute lung injury syndrome ...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/s41596-018-0019-4
更新日期：2018-08-01 00:00:00
abstract：:This protocol describes a method for converting short single-stranded and double-stranded DNA into libraries compatible with high-throughput sequencing using Illumina technology. This method has primarily been developed to improve sequence retrieval from ancient DNA, but it is also applicable to the sequencing of shor...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2013.038
更新日期：2013-04-01 00:00:00
abstract：:The transmembrane (TM) anchors of cell surface proteins have been one of the 'blind spots' in structural biology because they are generally very hydrophobic, sometimes dynamic, and thus difficult targets for structural characterization. A plethora of examples show these membrane anchors are not merely anchors but can ...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/s41596-019-0188-9
更新日期：2019-08-01 00:00:00
abstract：:Several noncanonical initial nucleotides (NCINs) have been found to cap RNAs and possibly regulate RNA stability, transcription and translation. NAD+ is one of the NCINs that has recently been discovered to cap RNAs in a wide range of species. Identification of the NAD+-capped RNAs (NAD-RNAs) could help to unveil the ...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/s41596-020-0363-z
更新日期：2020-09-01 00:00:00
abstract：:More than 400,000 people each year suffer adverse effects following bites from venomous snakes. However, snake venom is also a rich source of bioactive molecules with known or potential therapeutic applications. Manually 'milking' snakes is the most common method to obtain venom. Safer alternative methods to produce v...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/s41596-020-00463-4
更新日期：2021-01-27 00:00:00
abstract：:In the past few decades, marine natural products bioprospecting has yielded a considerable number of drug candidates. Two marine natural products have recently been admitted as new drugs: Prialt (also known as ziconotide) as a potent analgesic for severe chronic pain and Yondelis (known also as trabectedin or E-743) a...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2008.182
更新日期：2008-01-01 00:00:00
abstract：:Multipotent neural crest stem cells (NCSCs) have the potential to generate a wide range of cell types including melanocytes; peripheral neurons; and smooth muscle, bone, cartilage and fat cells. This protocol describes in detail how to perform a highly efficient, lineage-specific differentiation of human pluripotent c...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2012.156
更新日期：2013-01-01 00:00:00
abstract：:In this protocol, we describe cryoimmunolabeling methods for the subcellular localization of proteins and certain lipids. The methods start with chemical fixation of cells and tissue in formaldehyde (FA) and/or glutaraldehyde (GA), sometimes supplemented with acrolein. Cell and tissue blocks are then immersed in 2.3 M...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2007.365
更新日期：2007-01-01 00:00:00
abstract：:The development of single-molecule switching (SMS) fluorescence microscopy (also called single-molecule localization microscopy) over the last decade has enabled researchers to image cell biological structures at unprecedented resolution. Using two opposing objectives in a so-called 4Pi geometry doubles the available ...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/s41596-020-00428-7
更新日期：2020-12-16 00:00:00
abstract：:The fabrication of 3D tissues retaining the original functions of tissues/organs in vitro is crucial for optimal tissue engineering and regenerative medicine. The fabrication of 3D tissues also contributes to the establishment of in vitro tissue/organ models for drug screening. Our laboratory has developed a fabricati...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2012.027
更新日期：2012-04-05 00:00:00
abstract：:The Golgi apparatus undergoes extensive disassembly during mitosis and reassembly in post-mitotic daughter cells. This process has been mimicked in vitro by treating Golgi membranes with mitotic and interphase cytosol. To determine the minimal machinery that controls the morphological change, we have developed a defin...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2010.38
更新日期：2010-04-01 00:00:00
abstract：:Unlike transplantation of other solid organs, vascularized mouse lung transplantation has only recently been developed. In this protocol, we describe a detailed method for performing a vascularized and aerated mouse orthotopic lung transplant, which to date represents the most physiological mouse model of lung transpl...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2008.218
更新日期：2009-01-01 00:00:00
abstract：:In this protocol we describe how to perform the Multi-Source Interference Task (MSIT), a validated functional magnetic resonance imaging (fMRI) task that reliably and robustly activates the cingulo-frontal-parietal cognitive/attention network (CFP network) within individual subjects. The MSIT can be used to (i) identi...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2006.48
更新日期：2006-01-01 00:00:00
abstract：:Mitophagy is a cellular process that selectively removes damaged, old or dysfunctional mitochondria. Defective mitophagy is thought to contribute to normal aging and to various neurodegenerative and cardiovascular diseases. Previous methods used to detect mitophagy in vivo were cumbersome, insensitive and difficult to...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2017.060
更新日期：2017-08-01 00:00:00
abstract：:Postembryonic development is an important process of organismal maturation after embryonic growth. Despite key progress in recent years in understanding embryonic development via fluorescence time-lapse microscopy, comparatively less live-cell imaging of postembryonic development has been done. Here we describe a prot...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2012.128
更新日期：2012-12-01 00:00:00
abstract：:The plant cell wall is a chemically complex structure composed mostly of polysaccharides. Detailed analyses of these cell wall polysaccharides are essential for our understanding of plant development and for our use of plant biomass (largely wall material) in the food, agriculture, fabric, timber, biofuel and biocompo...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2012.081
更新日期：2012-09-01 00:00:00
abstract：:Growing evidence indicates that RNA G-quadruplexes have important roles in various processes such as transcription, translation, regulation of telomere length, and formation of telomeric heterochromatin. Investigation of RNA G-quadruplex structures associated with biological events is therefore essential to understand...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2017.156
更新日期：2018-04-01 00:00:00
abstract：:This protocol describes a culture system in which inner-ear sensory tissue is produced from mouse embryonic stem (ES) cells under chemically defined conditions. This model is amenable to basic and translational investigations into inner ear biology and regeneration. In this protocol, mouse ES cells are aggregated in 9...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2014.100
更新日期：2014-01-01 00:00:00
abstract：:Determining enantiomeric excess (e.e.) in chiral compounds is key to development of chiral catalyst auxiliaries and chiral drugs. Here we describe a sensitive and robust fluorescence-based assay for determining e.e. in mixtures of enantiomers of 1,2- and 1,3-diols, chiral amines, amino alcohols, and amino-acid esters....
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/s41596-020-0329-1
更新日期：2020-07-01 00:00:00
abstract：:The synthesis of an azobenzene amino acid (aa) for use as a photo-inducible conformational switch in polypeptides is described. The compound can be easily incorporated into an aa sequence by solid-phase peptide synthesis using standard 9-fluorenylmethoxycarbonyl methods. A reversible conformational change of the pepti...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2006.488
更新日期：2007-01-01 00:00:00
abstract：:The systematic mapping of protein interactions by bait-prey techniques, including affinity purification-mass spectrometry or the yeast two-hybrid system, contributes a unique and relevant perspective on the comprehensive picture of cellular machines. We describe here a protocol for statistical analysis of node-and-edg...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2009.26
更新日期：2009-01-01 00:00:00
abstract：:Neurofibromatosis type II (NF2) is a disease that lacks effective therapies. NF2 is characterized by bilateral vestibular schwannomas (VSs) that cause progressive and debilitating hearing loss, leading to social isolation and increased rates of depression. A major limitation in NF2 basic and translational research is ...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/s41596-018-0105-7
更新日期：2019-02-01 00:00:00
abstract：:Here, we describe a robust protocol for the reverse transfection of cells on small interfering (siRNA) arrays, which, in combination with multi-channel immunofluorescence or time-lapse microscopy, is suitable for genome-wide RNA interference (RNAi) screens in intact human cells. The automatic production of 48 'transfe...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2006.483
更新日期：2007-01-01 00:00:00
abstract：:In cell affinity chromatography, type-specific cell separation is based on the interaction between cell-surface receptors and an immobilized ligand on a stationary matrix. This protocol describes the preparation of monolithic polyacrylamide and polydimethylacrylamide cryogel affinity matrices that can be used as a gen...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2010.135
更新日期：2010-11-01 00:00:00
abstract：:In most organisms, one crossover (CO) event inhibits the chances of another nearby event. The term used to describe this phenomenon is 'CO interference'. Here, we describe a protocol for quickly generating large data sets that are amenable to CO interference analysis in the flowering plant, Arabidopsis thaliana. We em...
journal_title：Nature protocols
pub_type： 杂志文章
doi：10.1038/nprot.2007.491
更新日期：2008-01-01 00:00:00
