Abstract:
:Although lung transplant is a life-saving therapy for some patients, primary graft dysfunction (PGD) is a leading cause of mortality and morbidity soon after a transplant. Ischemia reperfusion injury is known to be one of the most critical factors in PGD development. PGD is by definition an acute lung injury syndrome that occurs during the first 3 d following lung transplantation. To successfully translate laboratory discoveries to clinical practice, a reliable and practical large animal model is critical. This protocol describes a surgical technique for swine lung transplantation and postoperative management for a further 3 d post transplant. The protocol includes the background and rationale, required supplies, and a detailed description of the donor operation, transplant surgery, postoperative care, and sacrifice surgery. A pig lung transplant model is reliably produced in which the recipients survive for 3 d post transplant. This 3-d survival model can be used by lung transplant researchers to assess the development of PGD and to test therapeutic strategies targeting PGD. In total, the protocol requires 5 h for the surgeries, plus ~2 h in total for the postoperative care.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Mariscal A,Caldarone L,Tikkanen J,Nakajima D,Chen M,Yeung J,Cypel M,Liu M,Keshavjee Sdoi
10.1038/s41596-018-0019-4subject
Has Abstractpub_date
2018-08-01 00:00:00pages
1814-1828issue
8eissn
1754-2189issn
1750-2799pii
10.1038/s41596-018-0019-4journal_volume
13pub_type
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