The immunoglobulin-like domains 1 and 2 of the protein tyrosine phosphatase LAR adopt an unusual horseshoe-like conformation.

abstract：:Initiation of transcription at malEp and malKp, two divergent Escherichia coli promoters, depends on the presence of both CRP, a pleiotropic activator, and MalT, the maltose regulon activator. We carried out in vivo genetic and functional analysis of these promoters and characterized their interaction with MalT and CR...

journal_title：Journal of molecular biology

authors： Raibaud O,Vidal-Ingigliardi D,Richet E

更新日期：1989-02-05 00:00:00

abstract：:The C(H)3 domain of antibodies is characterized by two antiparallel beta-sheets forming a disulfide-linked sandwich-like structure. At acidic pH values and low ionic strength, C(H)3 becomes completely unfolded. The addition of salt transforms the acid-unfolded protein into an alternatively folded state exhibiting a ch...

journal_title：Journal of molecular biology

authors： Thies MJ,Kammermeier R,Richter K,Buchner J

更新日期：2001-06-22 00:00:00

abstract：:To identify structural features in a G-protein-coupled receptor (GPCR) crucial for biosynthesis, stability in the membrane and stability in detergent micelles, we developed an evolutionary approach using expression in the inner membrane of Escherichia coli. From the analysis of 800,000 sequences of the rat neurotensin...

journal_title：Journal of molecular biology

authors： Schlinkmann KM,Hillenbrand M,Rittner A,Künz M,Strohner R,Plückthun A

更新日期：2012-09-21 00:00:00

abstract：:The sequence RGITVNGKTYGR has been reported as part of a de novo design peptide system. This peptide folds as a beta-hairpin structure with three residues per strand and two residue turns. Asn6 side-chain, the residue in position L1 of the beta-turn, appeared to be solvent exposed, interacting only within the turn but...

journal_title：Journal of molecular biology

authors： Ramírez-Alvarado M,Blanco FJ,Niemann H,Serrano L

更新日期：1997-11-07 00:00:00

abstract：:SCML2 (sex comb on midleg-like 2) is a constituent of the Polycomb repressive complex 1, a large multiprotein assembly required for the repression of developmental control genes. It contains two MBT (malignant brain tumor) repeats; the MBT is a protein module structurally similar to domains that bind to methylated his...

journal_title：Journal of molecular biology

authors： Santiveri CM,Lechtenberg BC,Allen MD,Sathyamurthy A,Jaulent AM,Freund SM,Bycroft M

更新日期：2008-10-24 00:00:00

abstract：:Recent computational studies of simple models of protein folding have concluded that a pronounced energy minimum (i.e. large gap in energy between low-energy states of the model) is a necessary and sufficient condition to ensure folding of a sequence to its lowest-energy conformation. Here, we show that this conclusio...

journal_title：Journal of molecular biology

authors： Unger R,Moult J

更新日期：1996-06-28 00:00:00

abstract：:The dependence of the electrophoretic mobility of small DNA rings on topological constraint was investigated in acrylamide or agarose gels as a function of DNA size (from approximately 350 to 1400 base-pairs), gel concentration and nucleotide sequence. Under appropriate adjustment between the size of the DNA and the g...

journal_title：Journal of molecular biology

authors： Zivanovic Y,Goulet I,Prunell A

更新日期：1986-12-05 00:00:00

abstract：:A protein microarray system containing different dilutions of 77 related and non-related proteins was used to show that IgE from subjects allergic to Brazil nut specifically recognise the seed 2S albumin protein (Ber e 1). Further, correctly folded chimaeric 2S albumin proteins containing structural epitope replacemen...

journal_title：Journal of molecular biology

authors： Alcocer MJ,Murtagh GJ,Wilson PB,Progias P,Lin J,Archer DB

更新日期：2004-10-22 00:00:00

abstract：:Proteasomes, major proteolytic sites in eukaryotic cells, play an important part in major histocompatibility class I (MHC I) ligand generation and thus in the regulation of specific immune responses. Their cleavage specificity is of outstanding interest for this process. In order to generalize previously determined cl...

journal_title：Journal of molecular biology

authors： Kuttler C,Nussbaum AK,Dick TP,Rammensee HG,Schild H,Hadeler KP

更新日期：2000-05-05 00:00:00

abstract：:We have studied the reversible dissociation of core size DNA from chicken erythrocyte nucleosome core particles in solutions containing 0 X 1 M to 0 X 6 M-NaCl. Dissociation increases with increasing NaCl concentration, increasing temperature and decreasing particle concentration. At high particle concentrations, no f...

journal_title：Journal of molecular biology

authors： Ausio J,Seger D,Eisenberg H

更新日期：1984-06-15 00:00:00

abstract：:Peptides that bind to major histocompatibility complex products (MHC) are known to exhibit certain sequence motifs which, though common, are neither necessary nor sufficient for binding: MHCs bind certain peptides that do not have the characteristic motifs and only about 30% of the peptides having the required motif, ...

journal_title：Journal of molecular biology

authors： Gulukota K,Sidney J,Sette A,DeLisi C

更新日期：1997-04-18 00:00:00

abstract：:The hydrogen exchange behaviour of two stabilised disulfide mutants of barnase has been examined using NMR H/2H exchange measurements. The choice of experimental conditions is crucial in experiments to study the effects of mutations on local and global stability and dynamic behaviour of proteins. If exchange condition...

journal_title：Journal of molecular biology

authors： Clarke J,Hounslow AM,Fersht AR

更新日期：1995-10-27 00:00:00

abstract：:The human signal recognition particle (SRP) is a large RNA-protein complex that targets secretory and membrane proteins to the endoplasmic reticulum membrane. The S domain of SRP is composed of roughly half of the 7SL RNA and four proteins (SRP19, SRP54, and the SRP68/72 heterodimer). In order to understand how the bi...

journal_title：Journal of molecular biology

authors： Menichelli E,Isel C,Oubridge C,Nagai K

更新日期：2007-03-16 00:00:00

abstract：:Ribosomes are dynamic complexes responsible for translating the genetic information encoded in mRNAs to proteins. The accuracy of this process is vital to the survival of an organism, and is often compromised by translational miscoding agents. Aminoglycosides are a group of miscoding agents that bind to the ribosome a...

journal_title：Journal of molecular biology

authors： Jerinic O,Joseph S

更新日期：2000-12-15 00:00:00

abstract：:A 3D reconstruction at 25 A resolution of native hemoglobin of the polychaete worm Arenicola marina was carried out from frozen-hydrated specimens examined in the electron microscope. The reconstruction volume of this large extracellular multimeric respiratory pigment appears as a hexagonal bilayer structure with ecli...

journal_title：Journal of molecular biology

authors： Jouan L,Taveau JC,Marco S,Lallier FH,Lamy JN

更新日期：2001-01-26 00:00:00

abstract：:The Holliday junction is the central intermediate in homologous recombination. Branch migration of this four-stranded DNA structure is a key step in genetic recombination that affects the extent of genetic information exchanged between two parental DNA molecules. Here, we have constructed synthetic Holliday junctions ...

journal_title：Journal of molecular biology

authors： Prabhu VP,Simons AM,Iwasaki H,Gai D,Simmons DT,Chen J

更新日期：2002-03-08 00:00:00

abstract：:Synthesis of asparaginyl-tRNA (Asn-tRNA(Asn)) in bacteria can be formed either by directly ligating Asn to tRNA(Asn) using an asparaginyl-tRNA synthetase (AsnRS) or by synthesizing Asn on the tRNA. In the latter two-step indirect pathway, a non-discriminating aspartyl-tRNA synthetase (ND-AspRS) attaches Asp to tRNA(As...

journal_title：Journal of molecular biology

authors： Nair N,Raff H,Islam MT,Feen M,Garofalo DM,Sheppard K

更新日期：2016-02-13 00:00:00

abstract：:Phosphoglucose isomerase (PGI; EC 5.3.1.9) is the second enzyme in glycolysis, where it catalyzes the isomerization of D-glucose-6-phosphate to D-fructose-6-phosphate. It is the same protein as autocrine motility factor, differentiation and maturation mediator, and neuroleukin. Here, we report a new X-ray crystal stru...

journal_title：Journal of molecular biology

authors： Arsenieva D,Jeffery CJ

更新日期：2002-10-11 00:00:00

abstract：:Although largely deemed as structurally conserved, catalytic metal ion sites can rearrange, thereby contributing to enzyme evolvability. Here, we show that in paraoxonase-1, a lipo-lactonase, catalytic promiscuity and divergence into an organophosphate hydrolase are correlated with an alternative mode of the catalytic...

journal_title：Journal of molecular biology

authors： Ben-David M,Wieczorek G,Elias M,Silman I,Sussman JL,Tawfik DS

更新日期：2013-03-25 00:00:00

abstract：:A DNA (cytosine)-5-methyltransferase from Haemophilus aegyptius (M.Hae III), which catalyzes methyl transfer from S-adenosyl-L-methionine to DNA, has been crystallized as a covalent complex with a suicide oligonucleotide substrate. Crystals of the co-complex were grown by vapor diffusion with hanging droplets, using p...

journal_title：Journal of molecular biology

authors： Reinisch KM,Chen L,Verdine GL,Lipscomb WN

更新日期：1994-05-13 00:00:00

abstract：:Androgen receptor (AR) is a ligand-dependent transcription factor and its activity is regulated by numerous AR coregulators. Aberrant expression of AR coregulators in prostate cancer cells has an important role in the development and progression of prostate cancer. We report here that CDC25A, a cell cycle-promoting ph...

journal_title：Journal of molecular biology

authors： Chiu YT,Han HY,Leung SC,Yuen HF,Chau CW,Guo Z,Qiu Y,Chan KW,Wang X,Wong YC,Ling MT

更新日期：2009-01-16 00:00:00

abstract：:Arginine-mediated regulation is remarkably well conserved in very divergent bacteria, and shows a number of unusual features that distinguish arginine regulation from other transcriptional control mechanisms. The arginine repressor subunit consists of a basic N-terminal DNA-binding domain, which belongs to the winged ...

journal_title：Journal of molecular biology

authors： Song H,Wang H,Gigot D,Dimova D,Sakanyan V,Glansdorff N,Charlier D

更新日期：2002-01-18 00:00:00

abstract：:A long-standing goal in biology is the complete annotation of function and structure on all protein-protein interactions, a large fraction of which is mediated by intrinsically disordered protein regions (IDRs). However, knowledge derived from experimental structures of such protein complexes is disproportionately sma...

journal_title：Journal of molecular biology

authors： Wong ETC,Gsponer J

更新日期：2019-08-09 00:00:00

abstract：:We have studied in reconstitution experiments the conditions under which peptides derived from histones H1 and H5 are bound in chromatin and to what extent they are involved in the organization of chromatin fibers. The fragments of rat liver histone H1 (rH1) and chicken erythrocytes H1 (cH1) and H5 (cH5) used were the...

journal_title：Journal of molecular biology

authors： Thoma F,Losa R,Koller T

更新日期：1983-07-05 00:00:00

abstract：:The Escherichia coli lac operon provides a classic paradigm for understanding regulation of gene transcription. It is now appreciated that lac promoter repression involves cooperative binding of the bidentate lac repressor tetramer to pairs of lac operators via DNA looping. We have adapted components of this system to...

journal_title：Journal of molecular biology

authors： Becker NA,Kahn JD,Maher LJ 3rd

更新日期：2005-06-17 00:00:00

abstract：:Cytochrome P450cam catalyzes the hydroxylation of camphor in a complex process involving two electron transfers (ETs) from the iron-sulfur protein putidaredoxin. The enzymatic control of the successive steps of catalysis is critical for a highly efficient reaction. The injection of the successive electrons is part of ...

journal_title：Journal of molecular biology

authors： Hiruma Y,Hass MA,Kikui Y,Liu WM,Ölmez B,Skinner SP,Blok A,Kloosterman A,Koteishi H,Löhr F,Schwalbe H,Nojiri M,Ubbink M

更新日期：2013-11-15 00:00:00

abstract：:The torsion constants of both circular and linear forms of the same 181 bp DNA were investigated by time-resolved fluorescence polarization anisotropy (FPA) of intercalated ethidium. The ratio of intrinsic ethidium binding constants of the circular and linear species was determined from the relative fluorescence inten...

journal_title：Journal of molecular biology

authors： Heath PJ,Clendenning JB,Fujimoto BS,Schurr JM

更新日期：1996-08-02 00:00:00

abstract：:Monoclonal antibodies recognize antigens with high affinity and specificity, but the structural basis for molecular mimicry remains unclear. It is often assumed that cross-reactive antigens share some structural similarity that is specifically recognized by a monoclonal antibody. Recent studies using combinatorial lib...

journal_title：Journal of molecular biology

authors： Pinilla C,Appel JR,Campbell GD,Buencamino J,Benkirane N,Muller S,Greenspan NS

更新日期：1998-11-13 00:00:00

abstract：:Using purified sigma 55 RNA polymerase from Bacillus subtilis in an in vitro transcription system, we have shown that both promoters and terminators of Gram negative origin are recognized by this enzyme. Furthermore, when B. subtilis is transformed with a shuttle vector containing certain of these promoters, synthesis...

journal_title：Journal of molecular biology

authors： Peschke U,Beuck V,Bujard H,Gentz R,Le Grice S

更新日期：1985-12-05 00:00:00

abstract：:One mechanism for the repair of UV-induced DNA damage is the base excision repair pathway. The initial step in this pathway and the specificity for the type of damage that is to be repaired reside in DNA glycosylase/abasic (AP) lyases. Cleavage of the glycosyl bond of the 5' pyrimidine of a cyclobutane pyrimidine dime...

journal_title：Journal of molecular biology

authors： Garvish JF,Lloyd RS

更新日期：2000-01-21 00:00:00