Abstract：
:A sensitive and specific liquid chromatography tandem mass spectrometry (HPLC-ESI(+)-MS/MS) procedure was developed and validated for the identification and quantification of thevetin B and further cardiac glycosides in human serum. The seeds of Yellow Oleander (Thevetia peruviana) contain cardiac glycosides that can cause serious intoxication. A mixture of six thevetia glycosides was extracted from these seeds and characterized. Thevetin B, isolated and efficiently purified from that mixture, is the main component and can be used as evidence. Solid phase extraction (SPE) proved to be an effective sample preparation method. Digoxin-d3 was used as the internal standard. Although ion suppression occurs, the limit of detection (LOD) is 0.27 ng/ml serum for thevetin B. Recovery is higher than 94%, and accuracy and precision were proficient. Method refinement was carried out with regard to developing a general screening method for cardiac glycosides. The assay is linear over the range of 0.5-8 ng/ml serum. Finally, the method was applied to a case of thevetia seed ingestion.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Kohls S,Scholz-Böttcher B,Rullkötter J,Teske Jdoi
10.1016/j.forsciint.2011.02.013subject
Has Abstractpub_date
2012-02-10 00:00:00pages
146-51issue
1-3eissn
0379-0738issn
1872-6283pii
S0379-0738(11)00075-2journal_volume
215pub_type
杂志文章abstract：:Medicolegal malpractice assessments have been an important part of the work of forensic pathologists. Not only botchers are concerned but also physicians of all clinical subjects, especially malpractice charges claiming a malpractice leading to death. Single long articles and book chapters have been published about ma...
journal_title：Forensic science international
pub_type： 历史文章,杂志文章
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更新日期：2004-09-10 00:00:00
abstract：:To assess the species composition of necrophagous blow flies (Diptera: Calliphoridae) in the area around the federal city of Switzerland, Bern, sampling with baited bottle traps was performed over the course of 34 weeks in 2014. Six locations ranging from urban to forest habitats were sampled weekly or rather biweekly...
journal_title：Forensic science international
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更新日期：2018-08-01 00:00:00
abstract：INTRODUCTION:Most individuals detained in police custody are male adolescents or young adults. Demeaning custody conditions, which are common in French police cells, bring to light legitimate questions about the detention of elderly people. We aimed to determine the proportion of detainees over age 60, their health cha...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2014.04.031
更新日期：2014-08-01 00:00:00
abstract：BACKGROUND:Studies testing the Willems method of age estimation with gender-specific scores on different population groups have reported consistently more accurate results over other methods. Not enough is known about the validity of Willems non-gender specific scores. AIM:The present study aimed to compare the accura...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2016.05.012
更新日期：2016-09-01 00:00:00
abstract：:The continuous emergence of NPS over the last years poses a series of novel challenges for forensic analysts. Most of those new compounds are synthesized with minimal chemical modifications to the structure of already known chemicals in order to avoid regulations. Some of these new compounds may undergo chemical chang...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2020.110306
更新日期：2020-07-01 00:00:00
abstract：:The AmpFlSTR Identifiler kit has recently been accepted for use in DNA databasing of forensic samples in the FBI's National DNA Index System. In the present study, we used this kit to analyze the allele distribution of 15 short tandem repeat markers (STR) in individuals living in Caracas city, Venezuela. The allele fr...
journal_title：Forensic science international
pub_type： 杂志文章
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journal_title：Forensic science international
pub_type： 杂志文章
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更新日期：2005-11-25 00:00:00
abstract：:A postmortem root band (PMRB) is a distinct microscopic feature that is postulated to occur in hair remaining in the follicle during the postmortem interval [1] (Petraco et al., 1998). The scientific validity of this premise has been highlighted in two recent high-profile criminal cases involving PMRBs [2,3] (State of...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2016.07.009
更新日期：2016-10-01 00:00:00
abstract：:The identification of body fluids at a crime scene is an important aspect of forensic casework analysis, being a source for investigative leads and contributing to case evidence. Yet, current methods for the forensic identification of body fluids suffer from several limitations, ranging from poor sensitivity and speci...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2018.07.013
更新日期：2018-09-01 00:00:00
abstract：:The AMPFlSTR((R)) SGM Plus system is a commercially available STR multiplex produced by Applied Biosystems, a division of Perkin Elmer, Foster City, California, USA that supersedes SGM. The multiplex contains the six SGM loci, amelogenin and four additional loci. These additional loci are D3S1358, D19S433, D16S539 and...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(00)00182-1
更新日期：2000-08-14 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(03)00110-5
更新日期：2003-06-24 00:00:00
abstract：:It is well-known that secular trends affect human stature and constitution, and this fact should be taken into consideration in forensic anthropology, especially in stature estimation. Recently, stature-group-specific equations have been developed to take into account these variations. The aim of the present study is ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.109973
更新日期：2019-11-01 00:00:00
abstract：:Foreign body aspiration is frequently encountered in young children, in the majority of cases it will not lead to an adverse outcome. However, in case of coin battery ingestion more serious adverse outcomes, including death, have been reported. We present a case with fatal outcome due to exsanguination, of a 2-year-ol...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2010.02.001
更新日期：2010-05-20 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2013.05.004
更新日期：2013-09-10 00:00:00
abstract：:The increasing interest in toxicological hair analysis as a marker of human exposure to xenobiotics such as illicit substances or therapeutic drugs, has been made feasible by the extension of mass spectrometry, a highly sensitive method of detection. A newborn exposed to drugs in utero can suffer from a varying degree...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(03)00050-1
更新日期：2003-04-23 00:00:00
abstract：:The aim of this study was to highlight the importance of evaluating entomological evidence in forensic investigations on a regional scale. To evaluate climatic, geographical and environmental influences on the selection of carrion-breeding fauna in Northern Italy and consequently on inferred forensic data (post-mortem...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2007.10.006
更新日期：2008-05-02 00:00:00
abstract：:Sudden unexplained death (SUD) constitutes a part of the overall sudden death that can not be underestimated. Over the last years, genetic testing on SUD has revealed that inherited channelopathies might play important roles in the pathophysiology of this disease. Ryanodine receptor type-2 (RYR2) is a kind of ion chan...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2016.12.005
更新日期：2017-01-01 00:00:00
abstract：:The aim of our study was to evaluate differences between males and females based on patterns of costal cartilage ossification and also with respect to ageing. We provided diagnosis of ossifications from two files of radiograms. The first group consisted of 1044 chest and abdominal radiograms of patients (537 men and 5...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2009.06.009
更新日期：2009-10-30 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2010.07.021
更新日期：2011-03-20 00:00:00
abstract：AIMS:The aim of this work is to test the stability of mephedrone added to whole blood collected from alive and dead mephedrone free-users and stored at three different temperatures (-20, +4 and +20°C) with and without preservatives up to 6 months, trying to establish the best storage condition in order to reduce possib...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2015.07.021
更新日期：2015-11-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2017.12.004
更新日期：2018-02-01 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2009.03.031
更新日期：2009-07-01 00:00:00
abstract：UNLABELLED:Objective Chloroprene, 2-chloro-1,3 butadiene, is a volatile synthetic liquid. The chloroprene monomer is extremely reactive and is used for the production of latexes and synthetic rubber such as Neoprene. Up to now an acute lethal human exposure has been described only once in the literature [19]. The intox...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2011.03.029
更新日期：2012-02-10 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2007.04.221
更新日期：2008-03-05 00:00:00
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journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2005.11.019
更新日期：2006-11-22 00:00:00
abstract：:An increasing number of drugs of abuse are sold word wide over the internet. Names like "legal highs", "herbal highs" etc. give the impression that these are safe products, although the risk of fatal reactions might be substantial. Leaves from the plant Mitragyna speciosa, contain active compounds like mitragynine and...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2014.10.025
更新日期：2014-12-01 00:00:00
abstract：:Reddish discoloration of exposed skin areas, called frost erythema, is an important criterion for the diagnosis of fatal hypothermia. In the present study, we used immunohistochemistry in a prospective trial to show that on the molecular level, the correlate of frost erythema is hemoglobin without hemorrhage. Furtherm...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2005.05.029
更新日期：2006-05-10 00:00:00
abstract：:The prosecution of those socially irresponsible individuals who drive when they have had too much to drink - in that their breath, blood or urine alcohol level exceeds the prescribed legal limit - has been standard practice since October 1967. The forensic analysis of whichever type of specimen is taken from the suspe...
journal_title：Forensic science international
pub_type： 杂志文章
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更新日期：2018-12-01 00:00:00
abstract：:Haplotype frequencies of eight Y-chromosome STR loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393 and DYS385) were determined from a sample of 285 unrelated males from Canary Islands. ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2004.05.004
更新日期：2005-03-10 00:00:00
abstract：:Bloodstain pattern analysts are sometimes called upon to determine the point of origin of a pattern of bloodstains. A derivation of expressions for the uncertainties deltaX and deltaY in the coordinates of the point of origin P(X, Y) of two bloodstains on a surface has recently been published. These uncertainties were...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2005.10.023
更新日期：2006-08-10 00:00:00
