Abstract:
:Cell storage in liquid nitrogen (LN) offers the most secure method of cell preservation even if cryopreserved cells are exposed to natural background of ionising radiation (IR). A lot of experiments have demonstrated that IR can induce damages in living cells, but only a little information regarding the response of cryopreserved cells is available. To investigate the effect of IR on frozen and unfrozen cells, peripheral blood mononuclear cells were directly irradiated at room temperature, then immediately frozen, or frozen and then irradiated in LN with different doses of gamma rays. After thawing, cells were incubated and death fraction was evaluated at different time points. Interestingly, the percentages of dead cells induced by IR gradually increased with both dose radiation and incubation time and were significantly lower for cells irradiated at -196°C than those irradiated at room temperature.
journal_name
Radiat Prot Dosimetryjournal_title
Radiation protection dosimetryauthors
Cugia G,Centis F,Del Zotto G,Lucarini A,Argazzi E,Zini G,Valentini M,Bono M,Picardi F,Stramigioli S,Cesarini W,Zamai Ldoi
10.1093/rpd/ncq541subject
Has Abstractpub_date
2011-02-01 00:00:00pages
237-40issue
2-4eissn
0144-8420issn
1742-3406pii
ncq541journal_volume
143pub_type
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