High survival of frozen cells irradiated with gamma radiation.

Abstract:

:Cell storage in liquid nitrogen (LN) offers the most secure method of cell preservation even if cryopreserved cells are exposed to natural background of ionising radiation (IR). A lot of experiments have demonstrated that IR can induce damages in living cells, but only a little information regarding the response of cryopreserved cells is available. To investigate the effect of IR on frozen and unfrozen cells, peripheral blood mononuclear cells were directly irradiated at room temperature, then immediately frozen, or frozen and then irradiated in LN with different doses of gamma rays. After thawing, cells were incubated and death fraction was evaluated at different time points. Interestingly, the percentages of dead cells induced by IR gradually increased with both dose radiation and incubation time and were significantly lower for cells irradiated at -196°C than those irradiated at room temperature.

journal_name

Radiat Prot Dosimetry

authors

Cugia G,Centis F,Del Zotto G,Lucarini A,Argazzi E,Zini G,Valentini M,Bono M,Picardi F,Stramigioli S,Cesarini W,Zamai L

doi

10.1093/rpd/ncq541

subject

Has Abstract

pub_date

2011-02-01 00:00:00

pages

237-40

issue

2-4

eissn

0144-8420

issn

1742-3406

pii

ncq541

journal_volume

143

pub_type

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