Abstract:
BACKGROUND:An earlier report described that transgenic mice ubiquitously expressing cryptochrome1 (CRY1) with a mutation in cystein414 (CRY1-AP Tg mice) display diabetes mellitus in addition to anomalous circadian behaviours. This study examined characteristic aspects of symptoms to clarify the diabetes type and pathogenesis. MATERIALS AND METHODS:The body weights and blood glucose levels of CRY1-AP Tg mice were measured for 7weeks starting at 3weeks after birth. Glucose tolerance test for the mice of various ages and insulin tolerance test at 6weeks of age were conducted. Immunohistochemical analysis of islets was carried out for the mice of 19 and 40weeks of age. Basal and glucose-stimulated serum insulin levels of mice at 27weeks were also measured. RESULTS:Three-week-old CRY1-AP Tg mice, which showed mild retardation in growth, already displayed glucose intolerance. Hyperglycaemia progressed with age, without accompanying insulin resistance. Insulin-stained areas in islets in CRY1-AP Tg mice were smaller than that in wild-type controls. Both basal and glucose-stimulated secretion of insulin decreased in CRY1-AP Tg mice. CONCLUSION:The symptoms of diabetes in CRY1-AP Tg mice turned out to be similar to those of maturity onset diabetes of the young (MODY) in humans in terms of early onset, non-obesity and primary dysfunction of beta cells. The CRY1-AP Tg mice might serve as an animal model of early onset insulin-secretory defect of diabetes.
journal_name
Eur J Clin Investjournal_title
European journal of clinical investigationauthors
Okano S,Hayasaka K,Igarashi M,Iwai H,Togashi Y,Nakajima Odoi
10.1111/j.1365-2362.2010.02359.xsubject
Has Abstractpub_date
2010-11-01 00:00:00pages
1011-7issue
11eissn
0014-2972issn
1365-2362pii
ECI2359journal_volume
40pub_type
杂志文章abstract::To determine the comparative value of serum Type III procollagen peptide (PIIIP) in paediatric and adult liver disease we have measured PIIIP in 201 healthy subjects (aged 1 day-77 years) and twenty-one children and five adults with chronic active hepatitis (CAH). Healthy children had significantly higher PIIIP levels...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1985.tb00147.x
更新日期:1985-04-01 00:00:00
abstract::The fate of noradrenaline released from sympathetic endings differs from that of noradrenaline secreted directly into the bloodstream. This has been used to establish a single sample test for the exclusion of phaeochromocytoma in patients with borderline elevation of plasma noradrenaline concentration. This test is ba...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1984.tb00706.x
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journal_title:European journal of clinical investigation
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abstract::Resealed erythrocytes are potential slow release carriers for drugs and enzymes. We have investigated carrier erythrocyte survival in human volunteers using gentamicin (G) as encapsulated cell marker; G was readily incorporated into red cells by hypo-osmotic dialysis (87% efficiency of incorporation) and did not exit ...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1986.tb01305.x
更新日期:1986-02-01 00:00:00
abstract:BACKGROUND:Morpholinosydnonimine, a nitric oxide (NO) donor, has been reported to inhibit insulin release in isolated pancreatic islets. We studied whether transdermal application of nitroglycerin, another NO donor widely used for angina prophylaxis, influenced glucose-stimulated insulin release in healthy, young, male...
journal_title:European journal of clinical investigation
pub_type: 临床试验,杂志文章,随机对照试验
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abstract::Inflammation is a well-coordinated process in response to tissue injury or infection aimed at restoration of tissue homoeostasis. Platelets and neutrophils are typically viewed important in the context of haemostasis and bacterial killing, respectively. However, as these cells are equipped with readily available armou...
journal_title:European journal of clinical investigation
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journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1046/j.1365-2362.1997.1700725.x
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journal_title:European journal of clinical investigation
pub_type: 杂志文章,多中心研究
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更新日期:2018-08-01 00:00:00
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journal_title:European journal of clinical investigation
pub_type: 杂志文章
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journal_title:European journal of clinical investigation
pub_type: 杂志文章,评审
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更新日期:2015-01-01 00:00:00
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journal_title:European journal of clinical investigation
pub_type: 杂志文章
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journal_title:European journal of clinical investigation
pub_type: 杂志文章
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journal_title:European journal of clinical investigation
pub_type: 杂志文章
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journal_title:European journal of clinical investigation
pub_type: 杂志文章
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journal_title:European journal of clinical investigation
pub_type: 杂志文章
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journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1046/j.1365-2362.2003.01148.x
更新日期:2003-06-01 00:00:00
abstract::To examine the possible effects of hyperoxalaemia on anaerobic metabolism and erythrocyte pyruvate kinase activity, we induced a rise in plasma oxalate in 11 dialysis patients by the oral administration of ascorbic acid, 500 mg day-1 for 3 weeks. Blood samples were taken from the same antecubital vein before and after...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1990.tb01878.x
更新日期:1990-08-01 00:00:00
abstract::Total serum haemolytic complement activity, plasma fibrinogen, erythrocyte sedimentation rate and other biological values in forty-three patients with Hodgkin's disease were correlated with results of staging. A highly significant increase (P=10(-5)) of the mean total serum haemolytic complement activity was found in ...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1977.tb01607.x
更新日期:1977-08-01 00:00:00
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journal_title:European journal of clinical investigation
pub_type: 杂志文章,评审
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journal_title:European journal of clinical investigation
pub_type: 杂志文章
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journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1984.tb01179.x
更新日期:1984-08-01 00:00:00
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journal_title:European journal of clinical investigation
pub_type: 杂志文章,评审
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更新日期:2015-10-01 00:00:00
abstract::Although in therapeutic use for more than a century, the mode of cellular action of organic nitrates remains incompletely understood. Despite ample experimental evidence from animal studies to show that nitrates are metabolized to NO in the vascular smooth muscle, direct demonstration of such an activity in human vasc...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1995.tb01952.x
更新日期:1995-10-01 00:00:00
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journal_title:European journal of clinical investigation
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更新日期:2013-12-01 00:00:00
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journal_title:European journal of clinical investigation
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2362.1993.tb00711.x
更新日期:1993-01-01 00:00:00
abstract::Platelet aggregation, which plays an important role in acute myocardial infarction (AMI), is mediated by fibrinogen binding to the platelet membrane glycoprotein (GP)IIb-IIIa (CD41). This study measured the relative number of GPIIb-IIIa complexes on platelets from patients immediately following AMI (n = 14) compared w...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1994.tb02062.x
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:In a previous study, we demonstrated the presence of receptors for somatostatin, a neuropeptide with immunoregulatory properties, in the inflammatory lesions of patients suffering from sarcoidosis and other granulomatous diseases by in vivo somatostatin receptor scintigraphy and in vitro autoradiography. How...
journal_title:European journal of clinical investigation
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更新日期:1999-07-01 00:00:00
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journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1991.tb01414.x
更新日期:1991-12-01 00:00:00
abstract:BACKGROUND:Vascular calcification (VC) is highly prevalent in patients with chronic kidney disease (CKD). Low magnesium levels are associated with VC, and recent in vitro studies confirm a protective role of magnesium, which is mediated by its entry into the VSMCs through the Transient Receptor Potential Melastatin 7 (...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/eci.12517
更新日期:2015-11-01 00:00:00
abstract:BACKGROUND:It remains unsettled whether alcoholic cirrhosis is a risk factor for myocardial infarction (MI). METHODS:We used data from nationwide healthcare registries to study all Danes diagnosed with alcoholic cirrhosis in 1996-2014, and five controls were matched to each of them on gender and age. We excluded every...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
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更新日期:2020-04-01 00:00:00