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A novel host-specific restriction system associated with DNA backbone S-modification in Salmonella.

Abstract：

:A novel, site-specific, DNA backbone S-modification (phosphorothioation) has been discovered, but its in vivo function(s) have remained obscure. Here, we report that the enteropathogenic Salmonella enterica serovar Cerro 87, which possesses S-modified DNA, restricts DNA isolated from Escherichia coli, while protecting its own DNA by site-specific phosphorothioation. A cloned 15-kb gene cluster from S. enterica conferred both host-specific restriction and DNA S-modification on E. coli. Mutational analysis of the gene cluster proved unambiguously that the S-modification prevented host-specific restriction specified by the same gene cluster. Restriction activity required three genes in addition to at least four contiguous genes necessary for DNA S-modification. This functional overlap ensures that restriction of heterologous DNA occurs only when the host DNA is protected by phosphorothioation. Meanwhile, this novel type of host-specific restriction and modification system was identified in many diverse bacteria. As in the case of methylation-specific restriction systems, targeted inactivation of this gene cluster should facilitate genetic manipulation of these bacteria, as we demonstrate in Salmonella.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Xu T,Yao F,Zhou X,Deng Z,You D

doi

10.1093/nar/gkq610

subject

Has Abstract

pub_date

2010-11-01 00:00:00

pages

7133-41

issue

20

eissn

0305-1048

issn

1362-4962

pii

gkq610

journal_volume

38

pub_type

杂志文章

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