Abstract:
:Metastatic tumor formation via vessel route begins with cancer cell extravasation from vessel lumen, migration into the connective tissue surrounding vessels, and invasion into target organ parenchyma. Epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET) have been recognized to play an important role in metastatic process, however, how and where these biological changes take place in the early phase of metastatic tumor development has never been clarified. We morphologically evaluated 34 small intrapulmonary metastases formed after cancer cell extravasation from lymphatics (lymphogenic metastasis) and 40 formed in the absence of extravasation (aerogenous metastasis) in human specimens and found that isolated or small clusters of invasive cancer cells (tumor budding) were frequently observed in lymphogenic metastasis (24/34; 71%), but were never observed within aerogenous metastasis. We immunostained 34 lymphogenic metastases for 13 molecular markers of EMT and MET and scored the immunostaining intensity of cancer cells floating in lymphatic vessels (LVs), migrating into the connective tissue surrounding vessels [bronchovascular bundle (BVB)], and growing in lung parenchyma (LP). Cancer cells within BVBs stained more weakly for E-cadherin (p < 0.001), β-catenin (p < 0.001), and Geminin (p < 0.001) and more strongly for MMP-7 (p = 0.046) and Laminin-5 γ2 (p = 0.037) than tumor cells in LVs. However, cancer cells in LP exhibited resurgent E-cadherin (p = 0.011), β-catenin (p < 0.001), and Geminin (p = 0.037) expression and reduced MMP-7 (p = 0.038) and Laminin-5 γ2 (p = 0.001) expression in comparison with cancer cells in BVBs. Our results suggested that in the early phase of metastatic tumor formation cancer cells undergo dynamic phenotypic change associated with EMT and subsequent MET.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Aokage K,Ishii G,Ohtaki Y,Yamaguchi Y,Hishida T,Yoshida J,Nishimura M,Nagai K,Ochiai Adoi
10.1002/ijc.25500subject
Has Abstractpub_date
2011-04-01 00:00:00pages
1585-95issue
7eissn
0020-7136issn
1097-0215journal_volume
128pub_type
杂志文章abstract::The growth of 7/9 Burkitt's lymphoma (BL) cell lines and 5/6 human melanoma cell lines was inhibited by certain human fibroblast-like cell lines, especially human myofibroblast cell lines. In contrast to BL lines, 3/5 lymphoblastoid cell lines were less susceptible and 3/4 human leukaemic lines were resistant. The eff...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910350410
更新日期:1985-04-15 00:00:00
abstract::Multiple myeloma, a neoplasm of plasma cells, accounts for approximately approximately 15% of lymphatohematopoietic cancers (LHC) and 2% of all cancers in the US. Incidence rates increase with age, particularly after age 40, and are higher in men, particularly African American men. The etiology is unknown with no esta...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.22718
更新日期:2007-01-01 00:00:00
abstract::Recent reports suggest that autoantibodies directed to aberrantly glycosylated mucins, in particular MUC1 and MUC4, are found in patients with colorectal cancer. There is, however, limited information on the autoantibody levels before clinical diagnosis, and their utility in cancer screening in the general population....
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28538
更新日期:2014-05-01 00:00:00
abstract::Somatic mutations in the TP53 gene are the most frequently observed genetic alterations in human malignancies, including breast cancer, which is one of the leading causes of death among women in Brazil. In our study, we determined the frequency and the pattern of TP53 mutations in malignant breast tumors from 120 pati...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10567
更新日期:2002-09-01 00:00:00
abstract::mTOR inhibition led to activation of upstream receptor tyrosine kinases (RTKs) and AKT, which may attenuate the efficacy of mTOR kinase inhibitors. We sought to discover efficient drug combination with mTOR inhibitors by elucidating the survival feedback loops induced by mTOR inhibition in breast cancer. The feedback ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28880
更新日期:2014-11-15 00:00:00
abstract::MAGE genes encoding tumor-rejection antigens recognized by cytotoxic T lymphocytes are expressed at the mRNA level in various malignant tumors. We have investigated the expression of genes MAGE-1, -2, -3/-6 and -4a/-4b at the mRNA level in malignant and non-malignant ovarian tumors as well as in normal ovaries by reve...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910640607
更新日期:1995-12-20 00:00:00
abstract::The application of glucocorticoids (GC3) in human leukemia is based on apoptosis induction but is often hampered by GC resistance. To delineate resistance mechanisms, we examined 5 GC-resistant leukemic cell lines, termed CEM-C7.R1-R5, isolated from the GC-sensitive human acute-T-cell-leukemic line, CCRF-CEM-C7, by se...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19961127)68:5<663::AID-IJC
更新日期:1996-11-27 00:00:00
abstract::Receptor status and gene amplification were studied in advanced human ovarian adenocarcinoma tissues, borderline and benign ovarian tumours and normal ovarian tissues. Sixty-five percent (53/82) of ovarian adenocarcinomas, 57% (8/14) of benign/borderline tumours and only 31% (5/16) of normal ovarian tissues studied sh...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910520211
更新日期:1992-09-09 00:00:00
abstract::The characteristics of tumor antigens of the transplantation type, TATA, were studied in a series of methylcholanthrene-induced sarcomas in pedigreed BALB/cAN mice. Each of the six sarcomas exhibited unique TATA when assayed for tumor rejection in syngeneic hosts. In three instances, however, in sarcomas CI-I, CII-5 a...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910250213
更新日期:1980-02-15 00:00:00
abstract::Parvoviruses of rodents are endowed with oncosuppressive properties. In particular, parvoviral infections protect host animals from spontaneous and chemical- or virus-induced tumour initiation in laboratory animals. The present study was undertaken to substantiate the capacity of parvovirus H-1 to inhibit therapeutica...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19980209)75:4<584::aid-ijc
更新日期:1998-02-09 00:00:00
abstract::Histologic characteristics of bladder cancer in Boston, USA, Manchester, UK, and Nagoya, Japan, were evaluated. In each of these areas broadly-based series of cases were assembled during a collaborative case-control study. The present analysis was based on 589 cases in Boston, 484 cases in Manchester, and 241 cases in...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910300605
更新日期:1982-12-15 00:00:00
abstract::A new cell line from a sporadic Wilms' tumor was established and extensively characterized. In nude mice, the tumor cells rapidly formed tumors which, in histological characteristics and extracellular-matrix (ECM) composition resembled sarcomatoid Wilms' tumor. The tumor cells produced B chains of laminin, but no A ch...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910540524
更新日期:1993-07-09 00:00:00
abstract::Seventy to 40% of K-RAS wild type colorectal tumors does not seem to benefit from treatment with antiepidermal growth factor receptor (anti-EGFR) monoclonal antibodies. Recent data suggested that in presence of IGF-1 system, altered activation colorectal cancer cells may escape anti-EGFR mediated cell death. The inter...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25193
更新日期:2010-10-15 00:00:00
abstract::Folate-mediated one-carbon metabolism plays critical roles in DNA synthesis, repair and DNA methylation. The impact of single nucleotide polymorphisms (SNPs) in folate-metabolizing enzymes has been investigated in risk of breast cancer among European or Asian populations, but not among women of African ancestry. We co...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29434
更新日期:2015-08-01 00:00:00
abstract::Three aspects of the rat model of HTLV-I/II infection were investigated. (i) The efficacy of HTLV-I-transformed rat cell lines in infecting different strains of rats: WKY and Lewis HTLV-I-transformed cell lines were injected into adult WKY, Lewis and Brown Norway rats, representing syngeneic and allogeneic combination...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19970926)73:1<131::aid-ijc
更新日期:1997-09-26 00:00:00
abstract::Prostate-specific antigen (PSA) has been characterized as a specific prostate marker, although recent studies have suggested the existence of PSA of nonprostatic sources. We have assessed the presence of PSA by an ultrasensitive assay in 276 serum and nonserum samples from women. Our data show the presence of PSA in 9...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19961115)68:4<424::AID-IJC
更新日期:1996-11-15 00:00:00
abstract::The aim of this register-based follow-up study was to evaluate the long-term cumulative incidence of second primary tumors (SPT) among survivors of hereditary retinoblastoma, with special interest for the incidence of pineoblastoma in retinoblastoma patients born after 1970. The Dutch Retinoblastoma Register was compl...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19960807)67:4<515::AID-IJC
更新日期:1996-08-07 00:00:00
abstract::Since advanced melanoma remains practically incurable, early detection is an important step toward a reduction in mortality. High expectations are entertained for a technique known as dermoscopy or epiluminescence light microscopy; however, evaluation of pigmented skin lesions by this method is often extremely complex...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10620
更新日期:2002-10-20 00:00:00
abstract::The roles of fibroblast growth factor-2 (FGF-2) in the hepatocellular carcinoma (HCC) development are still controversial. In this study, we investigated the expression of FGF-2 in chronic hepatitis (CH) type C patients with or without HCC and the immunoregulation of FGF-2 in NK sensitivity of HCC cells. The FGF-2 exp...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.26003
更新日期:2012-01-15 00:00:00
abstract::The lung retention patterns of B16 melanoma cells were determined after intravenous injection of [125l]dUrd-labelled tumor cells into B16 melanomabearing mice. Experiments were performed with mice undergoing acute or chronic reactions to bacterial endotoxin or zymosan, both of which were shown to modify the activity o...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910260118
更新日期:1980-07-15 00:00:00
abstract::The aim of our study was to develop and validate a machine learning algorithm to predict response of individual HER2-amplified colorectal cancer liver metastases (lmCRC) undergoing dual HER2-targeted therapy. Twenty-four radiomics features were extracted after 3D manual segmentation of 141 lmCRC on pretreatment portal...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.33271
更新日期:2020-12-01 00:00:00
abstract::Hormonal therapies targeting androgen receptor (AR) are effective in prostate cancer (PCa), but often the cancers progress to fatal castrate-resistant disease. Improved understanding of the cellular events during androgen deprivation would help to identify survival and stress pathways whose inhibition could synergize ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29303
更新日期:2015-06-01 00:00:00
abstract::In vitro, tumor-selective Hsp70 plasma membrane localization correlates with increased sensitivity to lysis mediated by a subpopulation of human natural killer (NK) cells that adhere to plastic following cytokine stimulation. In the present study, we analyzed the capacity of adoptively transferred human NK cells in SC...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/1097-0215(20001201)88:5<791::aid-ijc17>3.0
更新日期:2000-12-01 00:00:00
abstract::Melanoma is a highly malignant and increasingly common neoplasm. Because metastatic melanoma remains incurable, new treatment approaches are needed. Immunoliposomes have been previously shown to enhance the selective localization of immunoliposome-entrapped drugs to solid tumors with improvements in the therapeutic in...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19990412)81:2<268::aid-ijc
更新日期:1999-04-12 00:00:00
abstract::Cancer chemotherapy with platinum-based antineoplastic agents including oxaliplatin frequently results in a debilitating and painful peripheral neuropathy. We evaluated the antinociceptive effects of the alpha-2 adrenoceptor agonist, clonidine on oxaliplatin-induced neuropathic pain. Specifically, we determined if (i)...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29980
更新日期:2016-05-15 00:00:00
abstract::KLF5 is a transcription factor that plays important roles in multiple physical and pathological processes, including cell growth, cell cycle regulation, and angiogenesis. To better characterize KLF5 function in bladder carcinogenesis, we established stable TSU-Pr1 cell clones expressing different levels of KLF5. These...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21533
更新日期:2006-03-15 00:00:00
abstract::Professional antigen-presenting cells (APC), e.g. dendritic cells, express immuno-proteasome components and process proteins for MHC presentation differently from non-immune cells. Thus, they induce reactivities against sets of peptides that do not overlap with those generated by non-professional APC, i.e., tumor cell...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10616
更新日期:2002-10-01 00:00:00
abstract::We previously determined that human papillomavirus (HPV) virus-like particles (VLPs) and pseudovirions (PsV) did not, respectively, bind to or infect intact epithelium of the cervicovaginal tract. However, they strongly bound heparan sulfate proteoglycans (HSPG) on the basement membrane of disrupted epithelium and inf...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29823
更新日期:2016-02-15 00:00:00
abstract::Recombinant human (rhu) macrophage colony-stimulating factor (M-CSF) was evaluated for efficacy, either alone or in combination with local X-irradiation (LR), in mice inoculated subcutaneously (s.c.) with Lewis lung carcinoma (LLC) cells. The size of the primary tumor and numbers of lung metastases, 21 days after tumo...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910470125
更新日期:1991-01-02 00:00:00
abstract::Recently, pegylated arginine deiminase (ADI; EC 3.5.3.6) has been used to treat the patients with hepatocellular carcinoma or melanoma, in which the level of argininosuccinate synthetase (ASS) activity is low or undetectable. The efficacy of its antitumor activity largely depends on the level of intracellular ASS, whi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22322
更新日期:2007-02-15 00:00:00