Inherited XX sex reversal originating from wild medaka populations.

Abstract:

:The teleost fish, medaka (Oryzias latipes), has an XX/XY sex-determining mechanism. A Y-linked DM domain gene, DMY, has been isolated by positional cloning as the sex-determining gene in this species. Previously, we conducted a field survey of genotypic sex and found that approximately 1% of wild medaka are sex-reversed (XX males and XY females). Here, we performed genetic analyses of nine spontaneous XX sex-reversed males to elucidate its genetic basis. In all cases, the F(1) progeny were all females, whereas XX males reappeared in the backcross (BC) progeny, suggesting that XX sex reversal is a recessive trait. Although the incidences of sex reversal in the BC progeny were mostly low, 40% were males derived from one XX male. We performed linkage analysis using 55 BC males and located a single major factor, sda-1 (sex-determining autosomal factor-1), controlling sex reversal in an autosomal linkage group. Thus, genes involved in the sex-determining pathway can be isolated from spontaneous mutants in wild populations.

journal_name

Heredity (Edinb)

journal_title

Heredity

authors

Shinomiya A,Otake H,Hamaguchi S,Sakaizumi M

doi

10.1038/hdy.2010.51

subject

Has Abstract

pub_date

2010-11-01 00:00:00

pages

443-8

issue

5

eissn

0018-067X

issn

1365-2540

pii

hdy201051

journal_volume

105

pub_type

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