Abstract:
BACKGROUND:Laboratories traditionally culture toxoplasma tachyzoites in animals for testing and experimental use. This article considers why available cell culture methods are not used more often. AIM:To compare HeLa cell culture and animal culture for production of toxoplasma tachyzoites. METHODS:In 2000 HeLa culture replaced animal culture for continuous production of toxoplasma tachyzoites in the Scottish Toxoplasma Reference Laboratory. The performance of animal culture (1994-1998) was compared with HeLa culture (2004-2008). A PubMed search was carried out for 1998 and 2008 to assess the culture methods used in laboratories. RESULTS:Animal culture was able to produce higher yields of tachyzoites (10(9) from a cotton rat peritoneal harvest compared to 10(7) from a 75 cm(2) cell culture flask) but significantly more HeLa cultures were successful (93% versus 84%; p=0.025). There was no difference in the quality of tachyzoites from animal and HeLa cultures as demonstrated by the high levels of success in the dye test. HeLa culture offered significant advantages in flexibility and control. A review of the literature showed no significant change in the method of culture used in laboratories between 1998 and 2008 (p=0.36). CONCLUSION:The availability of cell culture methods and the increasingly stringent regulations on the use of animals have not resulted in a decline in the use of animal culture. Animals are necessary for certain experiments but many studies could use cell-culture-derived parasites.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Chatterton JM,McDonagh S,Ho-Yen DOdoi
10.1136/jcp.2009.072066subject
Has Abstractpub_date
2010-05-01 00:00:00pages
438-40issue
5eissn
0021-9746issn
1472-4146pii
jcp.2009.072066journal_volume
63pub_type
杂志文章abstract::The costs of a clinical chemistry laboratory in a district general hospital were studied. The system used has certain advantages over the conventional Cooper Lybrand method. The time taken by technicians to perform tests was more variable than expected and the cost of sample collection was higher than process-cost for...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.6.589
更新日期:1981-06-01 00:00:00
abstract::Publications describing aspects of the coccidian protozoan parasite Cryptosporidium, increased greatly during 1983 and 1984 as a result of not only increasing veterinary interest but also in the role of the parasite in the newly recognised acquired immune deficiency syndrome (AIDS). The reports reflected widespread co...
journal_title:Journal of clinical pathology
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abstract::Patients with congenital lung cysts are at increased risk of developing carcinoma, but the mechanisms concerned are not clear. The case of a young adult who developed a bronchioloalveolar carcinoma associated with a cystic congenital adenomatoid malformation is reported. The adjacent lung showed an unusual intra-alveo...
journal_title:Journal of clinical pathology
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journal_title:Journal of clinical pathology
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更新日期:2020-10-29 00:00:00
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journal_title:Journal of clinical pathology
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更新日期:2014-08-01 00:00:00
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journal_title:Journal of clinical pathology
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doi:10.1136/jcp.39.7.717
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journal_title:Journal of clinical pathology
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journal_title:Journal of clinical pathology
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doi:10.1136/jcp.42.11.1215
更新日期:1989-11-01 00:00:00
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journal_title:Journal of clinical pathology
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更新日期:1982-06-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
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更新日期:1992-10-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202363
更新日期:2014-11-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.11.1152
更新日期:1979-11-01 00:00:00
abstract::A radioimmunoassay for the measurement of gonadotrophin releasing hormone (GnRH) in plasma and urine using readily available reagents was developed. The GnRH assay showed good precision, recovery, and parallelism over a wide range of GnRH concentrations with a sensitivity of 15 pg/ml. The assay was compared with a com...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.42.5.542
更新日期:1989-05-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.24.3.263
更新日期:1971-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1973-07-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.7.676
更新日期:1995-07-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.44.7.576
更新日期:1991-07-01 00:00:00
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journal_title:Journal of clinical pathology
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更新日期:1993-04-01 00:00:00
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journal_title:Journal of clinical pathology
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更新日期:1986-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1136/jcp.2004.024182
更新日期:2005-07-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.22.3.263
更新日期:1969-05-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2004.022681
更新日期:2005-08-01 00:00:00
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更新日期:2002-05-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
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更新日期:2014-06-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
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更新日期:1961-03-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
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更新日期:1984-07-01 00:00:00