Abstract:
:Recent work demonstrated that a mannose receptor is involved in the phagocytosis of rod outer segments by the rat retinal pigment epithelium (RPE). In this study the binding of soluble mannose-containing ligands by human RPE explants is described. In addition, the authors report the isolation of a mannose receptor from human RPE and describe its relationship to the macrophage mannose receptor. Epithelial explants bound the soluble ligand 125I-mannose bovine serum albumin (BSA) by a mannose-specific process. The protein involved in mannose recognition was extracted from human tissue and purified using ligand-affinity chromatography. The protein that bound to the affinity column had a molecular weight of 175 kD by sodium dodecyl sulfate gel electrophoresis and migrated with the same mobility as the human macrophage mannose receptor. Antibodies directed against the macrophage receptor crossreacted with the mannose receptor from human RPE by immunoblot analysis. Binding specificity studies demonstrated that mannose and mannan inhibited ligand binding to the purified receptor by 65% and 90%, respectively; galactose had no effect. Using immunogold labeling of human RPE cells in explant culture, antimacrophage mannose receptor was localized at the apical plasma membrane. These results suggest that human RPE expresses a mannose receptor on its apical surface (as does the rat RPE) and that this receptor is similar to the human macrophage mannose receptor.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Shepherd VL,Tarnowski BI,McLaughlin BJsubject
Has Abstractpub_date
1991-05-01 00:00:00pages
1779-84issue
6eissn
0146-0404issn
1552-5783journal_volume
32pub_type
杂志文章abstract:PURPOSE:To determine the roles played by toll-like receptor 9 (TLR9) in cultured human corneal endothelial (HCEn) cells after herpes simplex virus type 1 (HSV-1) infection and to characterize the TLR9-mediated antiviral responses. METHOD:Immortalized HCEn cells were examined for TLR expression. The upregulation of inf...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.11-7805
更新日期:2011-08-24 00:00:00
abstract:PURPOSE:Generate site-specific somatic mutations selectively in the retinal pigment epithelium (RPE) in mice. METHODS:A transgenic mouse line expressing the Cre recombinase under the control of the tyrosinase-related protein (TRP)-1 promoter was generated. The presence of Cre was determined by in situ hybridization, a...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2002-05-01 00:00:00
abstract:PURPOSE:To evaluate how aging alters 24-hour measurements of intraocular pressure (IOP) in the sitting and supine body positions. METHODS:Fifteen older volunteers with healthy eyes (ages, 53-71 years) were each housed for 1 day in a sleep laboratory. An 8-hour accustomed sleep period was assigned to each subject. Ever...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.11-8763
更新日期:2012-01-10 00:00:00
abstract:Purpose:To quantify organelles impacting imaging in the cell body and intact apical processes of human retinal pigment epithelium (RPE), including melanosomes, lipofuscin-melanolipofuscin (LM), mitochondria, and nuclei. Methods:A normal perifovea of a 21-year-old white male was preserved after rapid organ recovery. An...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.61.8.13
更新日期:2020-07-01 00:00:00
abstract:Purpose:We describe the spatial distribution and cross-sectional associations with physical and lifestyle attributes. Methods:The participants of the Montrachet (Maculopathy Optic Nerve, nuTRition neurovAsCular and HEarT) study underwent an exhaustive ophthalmologic examination, including optical coherence tomography,...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章,多中心研究
doi:10.1167/iovs.16-19958
更新日期:2016-08-01 00:00:00
abstract:PURPOSE:Neuronal and glial alterations precede the overt vascular change that characterizes diabetic retinopathy. Because retinal astrocytes modulate neuronal and vascular function, this study investigated the time course of astrocyte, Müller cell, and neuronal change during diabetes to determine whether astrocytes may...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.11-7879
更新日期:2011-12-02 00:00:00
abstract:PURPOSE:Fluorescein and rose bengal are dyes used routinely in the examination of the ocular surface. As part of an ongoing search for a superior ophthalmic dye with optimal specificity and sensitivity and a lack of interference with subsequent viral cultures, and as part of studies that use chemical dyes to understand...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1994-03-01 00:00:00
abstract:PURPOSE:To determine whether the native basement membrane left behind after manual débridement wounding is retained throughout healing in the Balb/c mouse. METHODS:Mouse corneas were subjected to either 1.5 mm (small) or limbus-to-limbus (large) epithelial débridement wounds and allowed to heal for times ranging from ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
abstract:PURPOSE:To clarify the functions of a specific subtype of thyroid hormone receptor (TR), TRbeta2, and photoreceptor cell-specific nuclear receptor (PNR) in the development of cone photoreceptors. METHODS:The expression of short (S)- and medium (M)-wavelength cone opsins was analyzed by reverse transcription polymerase...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2002-11-01 00:00:00
abstract:PURPOSE:To investigate the residual stereo function of a group of 15 patients with strabismic amblyopia, by using motion-in-depth stimuli that allow discrimination of contributions from local disparity as opposed to those from local velocity mechanisms as a function of the rate of depth change. METHODS:The stereo perf...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.09-3551
更新日期:2009-10-01 00:00:00
abstract:PURPOSE:The mouse has become an important wound healing model with which to study corneal fibrosis, a frequent complication of refractive surgery. The aim of the current study was to quantify changes in stromal ultrastructure and light scatter that characterize fibrosis in mouse corneal debridement wounds. METHODS:Epi...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.11-9305
更新日期:2012-05-14 00:00:00
abstract:PURPOSE:Best disease is a monogenic macular degeneration caused mainly by heterozygous mutations in the BEST1 gene. The objective was to characterize the molecular and clinical features of patients with the classical form of Best disease that is inherited in an autosomal recessive mode. METHODS:Clinical evaluation inc...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.11-7174
更新日期:2011-07-18 00:00:00
abstract::The effects of x-rays and three chemical agents, known to cause intraocular inflammation in mammalian eyes, were studied on the chicken eye because this species was reported to be insensitive to the cataractogenic effects of x-rays. Intravitreal injection of Shigella endotoxin and topical and/or intravitreal administr...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1978-05-01 00:00:00
abstract::Light and electron microscopic studies were done on 11 cynomolgus monkey eyes which had undergone total iris removal followed by surgical disinsertion of the ciliary muscle from the scleral spur 4.7 to 14.4 months earlier. Anterior chamber perfusion to measure gross outflow facility had been performed one to nine time...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1977-03-01 00:00:00
abstract:PURPOSE:The influence of the inner wall of Schlemm's canal on aqueous outflow facility remains poorly understood. We examined the relationship between inner wall pore characteristics and outflow facility in living primate eyes in which facility had been pharmacologically increased by ethacrynic acid (ECA) infusion and ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract:Purpose:Pituitary adenylate cyclase activating polypeptide (PACAP) is neuroprotective in neuronal injuries. Bilateral common carotid artery occlusion (BCCAO) causes chronic hypoperfusion-induced degeneration in the rat retina, where we proved the retinoprotective effect of intravitreal PACAP. Although this route of adm...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.16-20630
更新日期:2016-12-01 00:00:00
abstract:PURPOSE:Endotoxin-induced uveitis (EIU) is a model that mimics human acute anterior uveitis. Cyclooxygenase (COX)-2 is an enzyme that initiates the conversion of arachidonic acid (AA) into prostaglandins (PGs), whereas 5-lipoxygenase (5-LO) generates leukotrienes (LT). The purpose of this study was to delineate the rol...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.03-0756
更新日期:2004-07-01 00:00:00
abstract:Purpose:In many individuals with pattern strabismus, the vertical misalignment varies with horizontal eye position. It has been proposed that these cross-axis effects result from abnormal cross-talk between brainstem structures that would normally encode horizontal and vertical eye position and velocity. The nucleus pr...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.61.5.45
更新日期:2020-05-11 00:00:00
abstract:PURPOSE:To conduct clinical and genetic studies in a European family with autosomal dominant Stargardt-like macular dystrophy (adSTGD-like MD) and to investigate the functional consequences of a novel ELOVL4 mutation. METHODS:Ophthalmic examination and mutation screening by direct sequencing of the ELOVL4 gene was per...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.04-0078
更新日期:2004-12-01 00:00:00
abstract::Autophagy and macropinocytosis are processes that are vital for cellular homeostasis, and help cells respond to stress and take up large amounts of material, respectively. The limbal and corneal epithelia have the machinery necessary to carry out both processes; however, autophagy and macropinocytosis are relatively u...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章,评审
doi:10.1167/iovs.16-21111
更新日期:2017-01-01 00:00:00
abstract:PURPOSE:To assess RNA stability after death in a porcine model to simulate current human eye bank techniques. METHODS:Eye bank time interval data were collected from 191 donor specimens: death to refrigeration, enucleation, and tissue processing. A control porcine eye was enucleated, retina and RPE isolated, and speci...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.02-1120
更新日期:2003-06-01 00:00:00
abstract:PURPOSE:Define a role for Fas-FasL in oxygen-induced retinopathy and explore the mechanism of pigment-epithelium-derived growth factor (PEDF) inhibition in this model. METHODS:Seven-day-old mice C57BL/6J (B6), FasL-defective (B6-gld), or Fas-defective (B6-lpr) mice were exposed to 75% oxygen for 5 days (postnatal day ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.02-0478
更新日期:2003-03-01 00:00:00
abstract:PURPOSE:To test the hypothesis that retinal hypoxia is present during vascular development in normal rat pups and in a newborn rat model of retinopathy of prematurity (ROP). METHODS:Preretinal vitreous PO(2) measurements were made during room air breathing using (19)F magnetic resonance spectroscopy (MRS) and a perflu...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.02-1122
更新日期:2003-07-01 00:00:00
abstract::The comparison of intracellular pH (pHi) regulation between fresh (FBCE) and cultured (CBCE) bovine corneal endothelium was extended to HCO3- transport mechanisms. Upon introduction of CO2/HCO3- Ringer's solution, there was a small, sharp acidification followed by an alkalinization .09 and .18 pH U above the HCO3- fre...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
abstract:Purpose:The purpose of this study was to clinically characterize patients with CNGA3-linked achromatopsia (CNGA3-ACHM) in preparation of a gene therapy trial. Methods:Thirty-six patients (age 7-56 years) with complete (cACHM) or incomplete (iACHM) CNGA3-ACHM were examined, including detailed psychophysical tests, exte...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.16-20427
更新日期:2017-02-01 00:00:00
abstract:PURPOSE:To determine factors affecting the disc-fovea angle (DFA), and to test the hypotheses that adjusting for DFA improves limits of retinal nerve fiber layer (RNFL) variability in normal subjects or enhances performance of RNFL measures for glaucoma detection. METHODS:Disc-fovea angle was measured on scanning lase...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.14-14962
更新日期:2014-10-09 00:00:00
abstract:Purpose:To assess the connection among arterioles, venules, and capillaries in three retinal capillary plexuses using optical coherence tomography angiography (OCTA). Methods:This was a prospective, cross-sectional, observational study including 20 eyes of 10 healthy subjects. En face and cross-sectional OCTA images w...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.18-24710
更新日期:2018-08-01 00:00:00
abstract:Purpose:To evaluate choroidal structural changes in eyes with myopic choroidal neovascularization (mCNV) treated with anti-VEGF over 12 months. Methods:We prospectively evaluated subfoveal choroidal thickness (SFCT) and choroidal vascularity index (CVI) using spectral-domain optical coherence tomography (SD-OCT) at ba...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.16-20191
更新日期:2016-09-01 00:00:00
abstract:PURPOSE:To describe a method for assessment and individualized compensation of anterior segment birefringence with scanning laser polarimetry. METHODS:A scanning laser polarimeter (GDx Nerve Fiber Analyzer; Laser Diagnostic Technologies, Inc., San Diego, CA) was modified to accommodate a variable compensator. The magn...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2002-07-01 00:00:00
abstract:PURPOSE:Age-related macular degeneration (AMD), the most common cause of incurable blindness in the western world, is characterized by the dysfunction and eventual death of choroidal endothelial (CECs), RPE, and photoreceptor cells. Stem cell-based treatment strategies designed to replace photoreceptor and RPE cells cu...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.15-17073
更新日期:2015-12-01 00:00:00