Abstract:
:We determined the feasibility of using scaffolds of adenoviral human BMP2 gene (AdBMP2)-modified human bone marrow mesenchymal stem cells (hBMSCs) and antigen-free bovine cancellous bone (BCB) to construct bone tissue. hMSCs were infected with AdBMP-2. Expression of BMP-2 and alkaline phosphatase confirmed successful secretion of active BMP-2. The osteogenic capability of a composite of AdBMP2-modified hMSCs with BCB was evaluated in athymic mice (group A). BCB (group B), hMSCs/BCB (group C), adenoviral beta-galactosidase genes (Adbetagal)-transfected hMSCs/BCB (group D) were controls. Formation of bone tissue was assessed by histological methods 4 weeks and 8 weeks after implantation. Implanted cells were identified by human Y-chromosome-specific fluorescence in-situ hybridization (FISH). hMSCs differentiated into osteogenic cells, and bone formation was observed. Obvious bone formation was not noted at any time point in control groups. We hypothesize that the described method is a promising method for bone regeneration.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Han D,Li J,Guan Xdoi
10.3109/03008200903318295subject
Has Abstractpub_date
2010-08-01 00:00:00pages
274-81issue
4eissn
0300-8207issn
1607-8438journal_volume
51pub_type
杂志文章abstract::We have investigated the changes in some of the biochemical and biophysical properties of human femoral head cartilage on the one hand during ageing and on the other hand in osteoarthritis. Topographical variations were also investigated. The parameters studied were those relevant to cartilage function, viz., proteogl...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208909043895
更新日期:1989-01-01 00:00:00
abstract:: Purpose/Aim of the study mice are the most often used pre-clinical lab models for studying the pathologies of bone mineralization. However, recent evidence suggests that two of the most often used mice strains (C57BL/6J and CD-1) might show differences in the bone minerali...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2019.1601184
更新日期:2020-09-01 00:00:00
abstract::Experimental lathyrism was induced by feeding newborn chicks a diet containing 0.2 and 0.4% DL-Penicillamine, with or without CuSO4 (10 mg/Kg diet) and Vitamin B6 (100 mg/Kg diet), or 0.015 and 0.1% beta-aminopropionitrile fumarate (beta-APN). After 7, 15, 25 and 55 days of treatment the animals were killed, the aorta...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208509015021
更新日期:1985-01-01 00:00:00
abstract::Two populations of primary osteoblasts and the cloned murine osteoblast cell line MC3T3 were activated with osteotropic agents and the conditioned media tested for the presence of macrophage colony stimulating factor (M-CSF). Differences in ability of the three populations to secrete the cytokine as well as in the eff...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208909023884
更新日期:1989-01-01 00:00:00
abstract::Purpose: Transplantation of autologous and/or allogeneic blood vessels is the most convenient treatment for vascular diseases. With regard to extensive need for blood vessels, developments in vascular tissue engineering are contributing greatly. In this study, our aim is to create intact small-diameter tubular vascula...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2019.1651848
更新日期:2021-03-01 00:00:00
abstract::Anterior cruciate ligament (ACL) has poor healing ability and an injured ACL would induce the degeneration of other intra-articular connective tissues. However, the coordinated expression and activities of matrix metalloproteinase (MMPs) in intra-articular tissues induced by ACL rupture were poorly understood. With a ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::A homogeneous preparation of glycoprotein (apparent molecular weight 34,000) has been isolated from bovine ligamentum nuchae by treatment of the tissue with 5 M guanidine HCl and 2-mercaptoethanol. The preparation exhibits amine oxidase activity which is only marginally inhibited by beta-aminopropionitrile. ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208109152380
更新日期:1981-01-01 00:00:00
abstract:AIM:To investigate the effect of extracellular matrix (ECM) proteins on characteristics of mesenchymal stem cells (MSCs) and tendon-derived cells (TDCs). MATERIALS AND METHODS:MSCs and TDCs, cultured in a monolayer (2D) or hydrogels (3D), with or without ECM protein supplementation, and on a non-viable native tendon (...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2016.1215442
更新日期:2016-11-01 00:00:00
abstract::For many years we have investigated the earliest crystal formations of different developing hard tissues (matrix vesicle, bone, dentine, enamel, etc.) by different electron microscopic measurements. It was observed that primarily Ca-phosphate (apatite) "chains," composed of nanometer sized particles (dots, islands), e...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.3109/03008209509016998
更新日期:1995-01-01 00:00:00
abstract::Purpose/Aim: Cementogenesis is a critical step in periodontal tissue regeneration given the essential role of cementum in anchoring teeth to the alveolar bone. This study is designed to achieve integrated cementum formation on the root surfaces of human teeth using growth factor-releasing scaffolds with periodontal li...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2016.1191478
更新日期:2016-11-01 00:00:00
abstract::Bone sialoprotein (BSP) expression is restricted to cells associated with the mineralization of bones and teeth. We previously identified a homeodomain binding element in a 2.5 kb fragment of the murine Bsp promoter that is required for osteoblast-selective expression in cell culture. To examine the role of this eleme...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::??Although injuries to the medial collateral ligament (MCL) can heal functionally without surgical intervention, the collagen fibers in the healing tissue remain compromised. The molecular basis for this poor healing potential was investigated by examining extracellular matrix-modifying molecules such as bone morphoge...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2010.524719
更新日期:2011-01-01 00:00:00
abstract::To investigate the source of bone brittleness in the disease osteogenesis imperfecta (OI), biomechanical properties have been measured in the femurs from a homozygous (oim/oim) mutant mouse model of OI, its heterozygous littermates, and wild-type animals. The novel technique of ultrasound critical-angle reflectometry ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209909005282
更新日期:1999-01-01 00:00:00
abstract::The protein components of biomineralized structures (matrix proteins) are believed to modulate crystal nucleation and growth, and thereby influence the shape and strength of the final structure. The chicken eggshell contains a complex array of distinct matrix proteins. One of these was found to have similar molecular ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209509010814
更新日期:1995-01-01 00:00:00
abstract::Scars formed after tendonitis result in altered tissue mechanical properties after injury. The interaction of collagen molecules with decorin affects collagen fibrogenesis, and scar tissue is fragile as a consequence of a large amount of decorin in the scar. We hypothesized that scar formation could be prevented by co...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200590935510
更新日期:2005-01-01 00:00:00
abstract::Muscle development in the embryonic chick involves the interfacing of two different cell types: myogenic cells forming the contractile network and connective tissue cells which wrap this network into discrete morphologies. Previous reports from this laboratory detailed the characterization of large extracellular matri...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208809015023
更新日期:1988-01-01 00:00:00
abstract:PURPOSE/AIM:Thyroid hormone has been implicated in the normal growth and development of articular cartilage; however, its effect on a disease state, such as hypothyroidism, is unknown. The purpose of this investigation was to compare normal articular cartilage from proximal femurs of immature miniature swine to proxima...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2020.1839436
更新日期:2020-12-11 00:00:00
abstract::Proteoglycans and hyaluronic acid synthesized by human skin fibroblasts in culture were characterized, and the effect of thyroid hormone deficiency was examined. The fibroblasts in culture synthesize hyaluronic acid, dermatan sulfate (DS) proteoglycans and heparan sulfate (HS) proteoglycans. Hyaluronic acid is almost ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208809015024
更新日期:1988-01-01 00:00:00
abstract::To study the glycoproteins and transglutaminase substrates involved in healing, wounds were made in the skin of anesthetized rats with a biopsy punch. Explants made 1-5 days later were incubated with [3H]-labelled putrescine, fucose or proline. As compared with unwounded skin there was an increased incorporation of la...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208709001994
更新日期:1987-01-01 00:00:00
abstract::Multiple hereditary exostoses (MHE) is an autosomal dominant disorder that affects about 1 in 50,000 children worldwide. MHE, also known as hereditary multiple exostoses (HME) or multiple osteochondromas (MO), is characterized by cartilage-capped outgrowths called osteochondromas that develop adjacent to the growth pl...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2017.1394295
更新日期:2018-01-01 00:00:00
abstract::An immunohistochemical analysis of the eggshell membranes shows the occurrence of type X collagen while type I collagen was not detected by using an appropriate monoclonal antibody with untreated shell membranes. A positive immuno-reaction for type I collagen was obtained after digestion of the shell membranes with pe...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209109152162
更新日期:1991-01-01 00:00:00
abstract::Scanning electron microscopy and high angle X-ray diffraction were used to define the relationship between collagen and elastin of bovine aortic wall. The diffraction pattern shows on one hand that the broad rings at 4.5 A and 9 A, due to elastin, do not orient on stretching and on the other hand, that the collagen ri...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207709152610
更新日期:1977-01-01 00:00:00
abstract::This study was designed to determine the effects of pulsed electromagnetic fields (PEMF) on the mRNA expression of the receptor activator of NF-kappa-B (RANK) and carbonic anhydrase II (CA II) in ovariectomized rat osteoclast-like cells. Marrow cells were harvested from femora and tibiae of rats, from which the ovarie...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200902855917
更新日期:2010-01-01 00:00:00
abstract::Bone sialoprotein (BSP) and osteopontin (OPN) are two extracellular bone matrix proteins that have the ability to modulate the growth of hydroxyapatite in vitro. Studies of BSP/OPN hydroxyapatite interactions in the past have been directed toward the identification of essential structural elements that allow these two...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209609029217
更新日期:1996-01-01 00:00:00
abstract::The turnover of proteoglycans in bovine articular cartilage was determined in explant cultures, maintained at 32 degrees C or 37 degrees C. Both the rate of proteoglycan synthesis and the release of newly synthesized proteoglycans were decreased in cultures incubated at 32 degrees C compared to 37 degrees C. At both t...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209109152166
更新日期:1991-01-01 00:00:00
abstract:AIM OF THE STUDY:To further elucidate the pathogenesis of systemic sclerosis (SSc) an experimental avian model was used. The University of California at Davis line 200 (UCD-200) chickens spontaneously develop a SSc-like disease that has most features of human SSc with vascular effects, inflammation, autoimmunity, and f...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2016.1182997
更新日期:2016-09-01 00:00:00
abstract::The expression of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) by human vascular smooth muscle cells (SMC) was monitored as a function of the phenotypic modulation in vitro. Cell phenotype was manipulated by varying serum concentration and cell density. Synthetic phenotype was characterized by a minim...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200009067666
更新日期:2000-01-01 00:00:00
abstract::DNA synthesis in rat and rabbit polyvinyl sponge induced granulation tissue has been studied using thymidine (methyl-3H). Synthesis was determined by measurement of thymidine incorporation into cold trichloroacetic acid insoluble material and by autoradiography. Granulation tissue was removed and immediately incubated...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207609152225
更新日期:1976-01-01 00:00:00
abstract::Aggrecan is the prominent proteoglycan in cartilage and is modified with approximately 100 chondroitin sulfate (CS) chains through a tetrasaccharide linkage structure. In osteoarthritis (OA), the viscoelastic properties of cartilage are compromised on both the quantity and integrity of aggrecan core protein expressed ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2012.756871
更新日期:2013-01-01 00:00:00
abstract::An agarose gel matrix was utilized to grow chondrocytes from human donors of various ages in cell culture. The chondrocytes produced the pericellular matrix characteristic for such cells and synthesized collagen type II as well as glyco-saminoglycans. The latter exhibit the typical distribution pattern of the respecti...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208609167140
更新日期:1986-01-01 00:00:00