Abstract:
BACKGROUND:MicroRNAs (miRNAs) are small noncoding regulatory RNAs whose aberrant expression may be observed in many malignancies. However, few data are yet available on human primary medulloblastomas. This work aimed to identify that whether miRNAs would be aberrantly expressed in tumor tissues compared with non-tumorous cerebellum tissues from same patients, and to explore a possible role during carcinogenesis. METHODS:A high throughput microRNA microarray was performed in human primary medulloblastoma specimens to investigate differentially expressed miRNAs, and some miRNAs were validated using real-time quantitative RT-PCR method. In addition, the predicted target genes for the most significantly down- or up-regulated miRNAs were analyzed by using a newly modified ensemble algorithm. RESULTS:Nine miRNA species were differentially expressed in medulloblastoma specimens versus normal non-tumorous cerebellum tissues. Of these, 4 were over expressed and 5 were under expressed. The changes ranged from 0.02-fold to 6.61-fold. These findings were confirmed using real-time quantitative RT-PCR for most significant deregulated miRNAs (miR-17, miR-100, miR-106b, and miR-218) which are novel and have not been previously published. Interestingly, most of the predicted target genes for these miRNAs were involved in medulloblastoma carcinogenesis. CONCLUSIONS:MiRNAs are differentially expressed between human medulloblastoma and non-tumorous cerebellum tissue. MiRNAs may play a role in the tumorigenesis of medulloblastoma and maybe serve as potential targets for novel therapeutic strategies in future.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Liu W,Gong YH,Chao TF,Peng XZ,Yuan JG,Ma ZY,Jia G,Zhao JZsubject
Has Abstractpub_date
2009-10-20 00:00:00pages
2405-11issue
20eissn
0366-6999issn
2542-5641journal_volume
122pub_type
杂志文章abstract:PURPOSE:To review the characteristics and evolution of the fungal spectrum, and the risk factors causing fungal infection, and to make progress in diagnosing fungal infection after organ transplantation. DATA SOURCES:An English-language literature search (MEDLINE 1990 - 2000) and bibliographic review of textbooks and ...
journal_title:Chinese medical journal
pub_type: 杂志文章,评审
doi:
更新日期:2003-09-01 00:00:00
abstract:BACKGROUND:Nontraumatic spontaneous subarachnoid hemorrhage (SAH) is associated with a high mortality. This study was conducted to investigate the epidemiological features of nontraumatic spontaneous SAH in China. METHODS:From January 2006 to December 2008, the clinical data of patients with nontraumatic SAH from 32 m...
journal_title:Chinese medical journal
pub_type: 杂志文章,多中心研究
doi:10.4103/0366-6999.202729
更新日期:2017-04-05 00:00:00
abstract:OBJECTIVE:To identify new recombinant antigens with potential for diagnosis of hemorrhagic fever with renal syndrome (HFRS) and establish reverse transcription-polymerase chain reaction-restriction fragments length polymorphism (RT-PCR-RFLP) for genotyping of hantavirus. METHODS:One group of primers was used to clone ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2001-10-01 00:00:00
abstract:BACKGROUND:Pancreatic beta-cell apoptosis induced by lipotoxicity, to a large extent, contributes to the progression of type 2 diabetes. To investigate the mechanism of free fatty acid induced apoptosis, we aimed to study the effects of palmitic acid (PA) on the apoptosis and peroxisome proliferator-activated receptor ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2011-11-01 00:00:00
abstract:OBJECTIVES:To examine whether the expression of choline acetyltransferase (ChAT), a marker for cholinergic neurons, and growth-associated protein (GAP-43), a marker for synaptogenesis, occurs differently following compression vs. decompression of spinal cord, and whether the reactions have any relationship with the tim...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract:BACKGROUND:Ahmed Glaucoma Valve implantation (AGVI) is used to treat refractory glaucoma. Breakdown of the blood-aqueous barrier (BAB) has been noted after some surgical techniques. The current study was designed to assess BAB disruption after AGVI. METHODS:Anterior chamber protein content was measured by the laser fl...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2013-03-01 00:00:00
abstract:BACKGROUND:Four single nucleotide polymorphisms (SNPs) in the modulator recognition factor 2/AT-rich interaction domain 5B (MRF2/ARID5B) gene located at chromosome 10q21.2 have been shown to be associated with both type 2 diabetes mellitus (T2DM) and coronary artery disease in a Japanese cohort. This study aimed to inv...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.204926
更新日期:2017-05-05 00:00:00
abstract::102 patients with nasal polyps receiving ND: YAG laser surgery or routine surgery were followed up for 18-36 months. The postoperative recurrence rates were 40.6% (24/54) and 66.6% (32/48) respectively for the patients of the two groups. Laser surgery is marked by less bleeding, no postoperative packing of the nose an...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00
abstract:BACKGROUND:The myocardial ATP sensitive potassium channel (K(ATP) channel) has been known for more than two decades, the properties of this channel have been intensively investigated, especially the myocardial protection effect by opening this channel. Numerous studies, including hypothermic, using K(ATP) agonists to a...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2006-10-20 00:00:00
abstract:OBJECTIVE:The aim of this study was to summarize recent studies on nondopaminergic options for the treatment of motor symptoms in Parkinson's disease (PD). DATA SOURCES:Papers in English published in PubMed, Cochrane, and Ovid Nursing databases between January 1988 and November 2016 were searched using the following k...
journal_title:Chinese medical journal
pub_type: 杂志文章,评审
doi:10.4103/0366-6999.211555
更新日期:2017-08-05 00:00:00
abstract:OBJECTIVES:To study the interrelation between nitric oxide (NO) and endothelin-1 (ET1) in experimental acute hypoxic rats, and to evaluate the mechanism of acute hypoxic pulmonary hypertension affected by NO and ET1 and its intervention. METHODS:Nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase histochemi...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract:BACKGROUND:Gastric intestinal metaplasia (GIM) is generally considered to be the main mucosal background for the development of gastric adenocarcinomas. Using linked color imaging (LCI), we noticed that the color pattern in areas of GIM was purple mixed with white on the epithelium with signs of mist that were detected...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.1097/CM9.0000000000000172
更新日期:2019-04-05 00:00:00
abstract::Pancreatic neuroendocrine tumors (NETs) are one subgroup of gastroenteropancreatic NETs. Its main characteristics are slow growth, frequent metastasis to the liver, and limited to the liver for long periods. In patients with irresectable liver metastatic NET, liver transplantation is the only radical treatment. About ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-08-01 00:00:00
abstract:BACKGROUND:Nucleostemin is essential for the proliferation and survival of stem and cancer cells, but it is unknown whether this newly identified molecule is involved in prostate cancer pathogenesis. METHODS:Total RNA and protein were extracted from prostate cancer tissues and PC-3, LNCap and DU145 cell lines. The nuc...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2008-02-20 00:00:00
abstract:BACKGROUND:The coordinated change of haematopoietic supporting microenvironment in bone marrow (BM) is crucial for innate immunity and inflammation. As the precursors of marrow stroma, BM derived mesenchymal stem cells (MSCs) promote haematopoietic function, but their roles in innate immunity or inflammation have not b...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2007-10-05 00:00:00
abstract:BACKGROUND:Methylmalonic acidemia (MMA) is a multifactorial autosomal recessive inborn error of organic acid metabolism, often presenting with neurological symptoms. As neurological disorders are often related to white matter injury, diffusion tensor imaging (DTI) is an excellent tool for assessment of white matter inj...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2009-04-20 00:00:00
abstract:OBJECTIVE:To review the current research into Foxp3(+) regulatory T cells (Treg) cell surface molecules, plasticity of Treg cells and mechanisms of Treg cell suppression and to explore the possibilities to interfere in Treg cell suppression of anti-tumor immunity. DATA SOURCES:A literature search of all English articl...
journal_title:Chinese medical journal
pub_type: 杂志文章,评审
doi:
更新日期:2010-11-01 00:00:00
abstract:OBJECTIVE:To study the changes of creatine kinase MM (CK-MM) isoforms in Duchenne muscular dystrophy (DMD) patients. METHODS:Serum samples from 49 DMD patients and 40 control subjects were collected for CK-MM isoforms measurement. CK-MM isoforms were separated within 30 minutes by electrophoresis on agarose gel with a...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND:Although transient ischemic attacks (TIA) is a clinical diagnosis, imaging findings are important for its diagnosis and treatment. This study evaluated the value and limitations of conventional CT, CT cerebral perfusion imaging and transcranial Doppler sonography (TCD) in patients with TIA. METHODS:Conventi...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2005-11-05 00:00:00
abstract:BACKGROUND:HATCH score is an established predictor of progression from paroxysmal to persistent atrial fibrillation (AF). The purpose of this study was to determine if HATCH score could predict recurrence after catheter ablation of AF. METHODS:The data of 488 consecutive paroxysmal AF patients who underwent an index c...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-10-01 00:00:00
abstract::The solid pseudopapillary tumors of the pancreas (SPTP) are rare tumors, which are commonly found in adolescent women. Radical surgical resection of the primary tumor or metastases is the standard treatment for SPTP and could achieve long-term survival. We reported a case of a 20-year-old female with multiple liver me...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-05-01 00:00:00
abstract:OBJECTIVE:To investigate the complications of laparoscopic cholecystectomy in China. METHODS:All Chinese articles about laparoscopic cholecystectomy published between April 1994 and November 1995 were identified through CMCC (Chinese Medical Computerized Contents). From more than 600 titles, 105 articles were screened...
journal_title:Chinese medical journal
pub_type: 杂志文章,评审
doi:
更新日期:1997-09-01 00:00:00
abstract:BACKGROUND:Endogenous hydrogen sulfide is a new neuromodulator which takes part in the regulation of central nervous system physiology and diseases. Whether endogenous hydrogen sulfide in the central nervous system regulates cardiovascular activity is not known. In the present study, we observed the hemodynamic changes...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2011-11-01 00:00:00
abstract:BACKGROUND:The emergence of bacterial resistance to commonly used antibiotics, such as macrolides, is complicating the management of respiratory tract infections (RTIs). Telithromycin, a ketolide antimicrobial structurally related to macrolides, is approved for the treatment of community-acquired RTIs, and shows lower ...
journal_title:Chinese medical journal
pub_type: 杂志文章,meta分析
doi:
更新日期:2013-01-01 00:00:00
abstract::Anti-HNK-1 monoclonal antibody (MAb) was reactive with non-cancerous and cancerous prostatic epithelia, as well as natural killer cells, myelinated nerves and cells from APUD systems. The expression of HNK-1 antigen on prostate cancer was investigated immunohistochemically by avidinbiotin-peroxidase complex (ABC) meth...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1995-07-01 00:00:00
abstract:OBJECTIVE:To understand the relationship among congenital choledochal cyst, anomalous junction of pancreaticobiliary duct (AJPBD) and pancreatitis. METHODS:25 children with choledochal cyst treated in our hospital were included in this study. Their ages ranged from 2 months to 14 years. Intraoperative cholangiography ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract::Apolipoprotein (a) [Lp(a)] phenotypes of 69 myocardial infarction survivor and 56 stroke patients were reported and compared to those of 190 healthy Chinese. The results revealed that the distributions of apo(a) phenotype frequency in patients with cardio-cerebrovascular disease (CCVD) were different from those of con...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1994-02-01 00:00:00
abstract:OBJECTIVE:To investigate the incidence rate, pathologic changes, mechanisms and diagnostic methods in renal bone disease. METHODS:The blood levels of carboxyterminal parpthyriod hormone (C-PTH), 1,25(OH)2D3, calcium and phosphate, aluminum in serum and bone tissue were measured. The bone biopsy and bone scan with 99m ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract:BACKGROUND:Despite the growing epidemic of heart failure (HF), there is limited data available to systematically compare non-cardiac comorbidities in the young-old, old-old, and oldest-old patients hospitalized for HF. The precise differences will add valuable information for better management of HF in elderly patients...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.1097/CM9.0000000000000560
更新日期:2019-12-20 00:00:00
abstract:BACKGROUND:T8590C polymorphism of CYP4A11 has been associated with hypertension, though with conflicting results. The aim of this study was to quantitatively summarize the evidence for CYP4A11 T8590C polymorphism and hypertension risk. METHODS:Electronic search of PubMed and the Chinese Biomedicine database was conduc...
journal_title:Chinese medical journal
pub_type: 杂志文章,meta分析
doi:
更新日期:2014-01-01 00:00:00