Ancient DNA reveals late survival of mammoth and horse in interior Alaska.

Abstract:

:Causes of late Quaternary extinctions of large mammals ("megafauna") continue to be debated, especially for continental losses, because spatial and temporal patterns of extinction are poorly known. Accurate latest appearance dates (LADs) for such taxa are critical for interpreting the process of extinction. The extinction of woolly mammoth and horse in northwestern North America is currently placed at 15,000-13,000 calendar years before present (yr BP), based on LADs from dating surveys of macrofossils (bones and teeth). Advantages of using macrofossils to estimate when a species became extinct are offset, however, by the improbability of finding and dating the remains of the last-surviving members of populations that were restricted in numbers or confined to refugia. Here we report an alternative approach to detect 'ghost ranges' of dwindling populations, based on recovery of ancient DNA from perennially frozen and securely dated sediments (sedaDNA). In such contexts, sedaDNA can reveal the molecular presence of species that appear absent in the macrofossil record. We show that woolly mammoth and horse persisted in interior Alaska until at least 10,500 yr BP, several thousands of years later than indicated from macrofossil surveys. These results contradict claims that Holocene survival of mammoths in Beringia was restricted to ecologically isolated high-latitude islands. More importantly, our finding that mammoth and horse overlapped with humans for several millennia in the region where people initially entered the Americas challenges theories that megafaunal extinction occurred within centuries of human arrival or were due to an extraterrestrial impact in the late Pleistocene.

authors

Haile J,Froese DG,Macphee RD,Roberts RG,Arnold LJ,Reyes AV,Rasmussen M,Nielsen R,Brook BW,Robinson S,Demuro M,Gilbert MT,Munch K,Austin JJ,Cooper A,Barnes I,Möller P,Willerslev E

doi

10.1073/pnas.0912510106

subject

Has Abstract

pub_date

2009-12-29 00:00:00

pages

22352-7

issue

52

eissn

0027-8424

issn

1091-6490

pii

0912510106

journal_volume

106

pub_type

历史文章,杂志文章
  • Light-induced psbA translation in plants is triggered by photosystem II damage via an assembly-linked autoregulatory circuit.

    abstract::The D1 reaction center protein of photosystem II (PSII) is subject to light-induced damage. Degradation of damaged D1 and its replacement by nascent D1 are at the heart of a PSII repair cycle, without which photosynthesis is inhibited. In mature plant chloroplasts, light stimulates the recruitment of ribosomes specifi...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2007833117

    authors: Chotewutmontri P,Barkan A

    更新日期:2020-09-01 00:00:00

  • The formation of mitochondrial ribonucleoprotein complexes involving guide RNA molecules in Trypanosoma brucei.

    abstract::Transcripts from mitochondrial (maxicircle) genes of kinetoplastid organisms undergo RNA editing characterized by a series of reactions that insert and delete uridine nucleotides within the sequence of the pre-mRNAs. Guide RNAs, which complement fully edited mRNAs, provide the information for the edited sequence by an...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.5.1776

    authors: Göringer HU,Koslowsky DJ,Morales TH,Stuart K

    更新日期:1994-03-01 00:00:00

  • Unliganded gating of acetylcholine receptor channels.

    abstract::We estimated the unliganded opening and closing rate constants of neuromuscular acetylcholine receptor-channels (AChRs) having mutations that increased the gating equilibrium constant. For some mutant combinations, spontaneous openings occurred in clusters. For 25 different constructs, the unliganded gating equilibriu...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0809272106

    authors: Purohit P,Auerbach A

    更新日期:2009-01-06 00:00:00

  • Hypophagia and metabolic adaptations in mice with defective ATGL-mediated lipolysis cause resistance to HFD-induced obesity.

    abstract::Adipose triglyceride lipase (ATGL) initiates intracellular triglyceride (TG) catabolism. In humans, ATGL deficiency causes neutral lipid storage disease with myopathy (NLSDM) characterized by a systemic TG accumulation. Mice with a genetic deletion of ATGL (AKO) also accumulate TG in many tissues. However, neither NLS...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1516004112

    authors: Schreiber R,Hofer P,Taschler U,Voshol PJ,Rechberger GN,Kotzbeck P,Jaeger D,Preiss-Landl K,Lord CC,Brown JM,Haemmerle G,Zimmermann R,Vidal-Puig A,Zechner R

    更新日期:2015-11-10 00:00:00

  • Direct imaging of nanoscale magnetic interactions in minerals.

    abstract::The magnetic microstructure of a natural, finely exsolved intergrowth of submicron magnetite blocks in an ulvöspinel matrix is characterized by using off-axis electron holography in the transmission electron microscope. Single-domain and vortex states in individual blocks, as well as magnetostatic interaction fields b...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.262514499

    authors: Harrison RJ,Dunin-Borkowski RE,Putnis A

    更新日期:2002-12-24 00:00:00

  • Protein trans-splicing in transgenic plant chloroplast: reconstruction of herbicide resistance from split genes.

    abstract::Inteins are intervening protein sequences that undergo self-excision from a precursor protein with concomitant joining of the flanking sequences. Here, we demonstrate intein trans-splicing in Nicotiana tabacum chloroplasts by using the naturally split Ssp DnaE intein. Trans-splicing occurred whether both intein fragme...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0736538100

    authors: Chin HG,Kim GD,Marin I,Mersha F,Evans TC Jr,Chen L,Xu MQ,Pradhan S

    更新日期:2003-04-15 00:00:00

  • Homozygously deleted gene DACH1 regulates tumor-initiating activity of glioma cells.

    abstract::Loss or reduction in function of tumor suppressor genes contributes to tumorigenesis. Here, by allelic DNA copy number analysis using single-nucleotide polymorphism genotyping array and mass spectrometry, we report homozygous deletion in glioblastoma multiformes at chromosome 13q21, where DACH1 gene is located. We fou...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0906930108

    authors: Watanabe A,Ogiwara H,Ehata S,Mukasa A,Ishikawa S,Maeda D,Ueki K,Ino Y,Todo T,Yamada Y,Fukayama M,Saito N,Miyazono K,Aburatani H

    更新日期:2011-07-26 00:00:00

  • Voltage-sensitive calcium channels regulate guanosine 3',5'-cyclic monophosphate levels in neuroblastoma cells.

    abstract::Veratridine or high potassium concentration increased guanosine 3',5'-cyclic monophosphate (cGMP) levels in neuroblastoma cells of clone N1E-115 without affecting levels of adenosine 3',5'-cyclic monophosphate (cAMP). The increases in cGMP appear to be a direct result of the depolarizing action of these agents and not...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.75.12.6295

    authors: Study RE,Breakefield XO,Bartfai T,Greengard P

    更新日期:1978-12-01 00:00:00

  • Frequency selectivity of synaptic exocytosis in frog saccular hair cells.

    abstract::The ability to respond selectively to particular frequency components of sensory inputs is fundamental to signal processing in the ear. The frog (Rana pipiens) sacculus, which is used for social communication and escape behaviors, is an exquisitely sensitive detector of sounds and ground-borne vibrations in the 5- to ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0511005103

    authors: Rutherford MA,Roberts WM

    更新日期:2006-02-21 00:00:00

  • Inhibition of DUX4 expression with antisense LNA gapmers as a therapy for facioscapulohumeral muscular dystrophy.

    abstract::Facioscapulohumeral muscular dystrophy (FSHD), characterized by progressive muscle weakness and deterioration, is genetically linked to aberrant expression of DUX4 in muscle. DUX4, in its full-length form, is cytotoxic in nongermline tissues. Here, we designed locked nucleic acid (LNA) gapmer antisense oligonucleotide...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1909649117

    authors: Lim KRQ,Maruyama R,Echigoya Y,Nguyen Q,Zhang A,Khawaja H,Sen Chandra S,Jones T,Jones P,Chen YW,Yokota T

    更新日期:2020-07-14 00:00:00

  • Catheterization alters bladder ecology to potentiate Staphylococcus aureus infection of the urinary tract.

    abstract::Methicillin-resistant Staphylococcus aureus (MRSA) is an emerging cause of catheter-associated urinary tract infection (CAUTI), which frequently progresses to more serious invasive infections. We adapted a mouse model of CAUTI to investigate how catheterization increases an individual's susceptibility to MRSA UTI. Thi...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1707572114

    authors: Walker JN,Flores-Mireles AL,Pinkner CL,Schreiber HL 4th,Joens MS,Park AM,Potretzke AM,Bauman TM,Pinkner JS,Fitzpatrick JAJ,Desai A,Caparon MG,Hultgren SJ

    更新日期:2017-10-10 00:00:00

  • Identification of biological molecules in situ at high resolution via the fluorescence excited by a scanning electron beam.

    abstract::Proteins, nucleic acids, and fluorescein-conjugated antibody are shown to be identifidable in situ via the fluorescence excited by the focused electron beam of a canning electron microscope. A molecular species is identified by its characteristic fluorescence spectrum and by a characteristic alteration of the spectrum...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.73.2.317

    authors: Hough PV,McKinney WR,Ledbeter MC,Pollack RE,Moos HW

    更新日期:1976-02-01 00:00:00

  • gamma-Aminobutyric acid is an inhibitory neurotransmitter restricting the release of luteinizing hormone-releasing hormone before the onset of puberty.

    abstract::To test the hypothesis that the pubertal increase in luteinizing hormone-releasing hormone (LHRH) release is withheld by a dominant inhibitory neuronal system, the role of gamma-aminobutyric acid (GABA), a known inhibitory neurotransmitter, in the control of LHRH release was examined in conscious female monkeys at the...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.1.395

    authors: Mitsushima D,Hei DL,Terasawa E

    更新日期:1994-01-04 00:00:00

  • Selective activation of the M1 muscarinic acetylcholine receptor achieved by allosteric potentiation.

    abstract::The forebrain cholinergic system promotes higher brain function in part by signaling through the M(1) muscarinic acetylcholine receptor (mAChR). During Alzheimer's disease (AD), these cholinergic neurons degenerate, therefore selectively activating M(1) receptors could improve cognitive function in these patients whil...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0900903106

    authors: Ma L,Seager MA,Wittmann M,Jacobson M,Bickel D,Burno M,Jones K,Graufelds VK,Xu G,Pearson M,McCampbell A,Gaspar R,Shughrue P,Danziger A,Regan C,Flick R,Pascarella D,Garson S,Doran S,Kreatsoulas C,Veng L,Lindsley C

    更新日期:2009-09-15 00:00:00

  • 5'-Terminal sequences and coding region of late simian virus 40 mRNAs are derived from noncontiguous segments of the viral genome.

    abstract::The region of the simian virus 40 genome complementary to the 5' end of the most abundant poly(A)-containing 19S and 16S mRNAs was mapped by hybridization of double-labeled RNA ([3H]methyl group and [14C]uridine) to specific DNA fragments. Chemical identification of methylated residues indicated that a common "leader"...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.74.12.5323

    authors: Lavi S,Groner Y

    更新日期:1977-12-01 00:00:00

  • Adenovirus-mediated interleukin-12 gene therapy for metastatic colon carcinoma.

    abstract::Recombinant adenoviral mediated delivery of suicide and cytokine genes has been investigated as a treatment for hepatic metastases of colon carcinoma in mice. Liver tumors were established by intrahepatic implantation of a poorly immunogenic colon carcinoma cell line (MCA-26), which is syngeneic in BALB/c mice. Intrat...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.93.21.11302

    authors: Caruso M,Pham-Nguyen K,Kwong YL,Xu B,Kosai KI,Finegold M,Woo SL,Chen SH

    更新日期:1996-10-15 00:00:00

  • Disruption of Lrp4 function by genetic deletion or pharmacological blockade increases bone mass and serum sclerostin levels.

    abstract::We identified previously in vitro LRP4 (low-density lipoprotein receptor-related protein 4) as a facilitator of the WNT (Wingless-type) antagonist sclerostin and found mutations disrupting this function to be associated with high bone mass in humans similar to patients lacking sclerostin. To further delineate the role...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1413828111

    authors: Chang MK,Kramer I,Huber T,Kinzel B,Guth-Gundel S,Leupin O,Kneissel M

    更新日期:2014-12-02 00:00:00

  • Entropy in molecular recognition by proteins.

    abstract::Molecular recognition by proteins is fundamental to molecular biology. Dissection of the thermodynamic energy terms governing protein-ligand interactions has proven difficult, with determination of entropic contributions being particularly elusive. NMR relaxation measurements have suggested that changes in protein con...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1621154114

    authors: Caro JA,Harpole KW,Kasinath V,Lim J,Granja J,Valentine KG,Sharp KA,Wand AJ

    更新日期:2017-06-20 00:00:00

  • Identification of an IL-27/osteopontin axis in dendritic cells and its modulation by IFN-gamma limits IL-17-mediated autoimmune inflammation.

    abstract::Dendritic cells (DCs) play a central role in determining the induction of T cell responses. IL-27 production by DCs favors induction of IL-10-producing regulatory T cells, whereas osteopontin (OPN) promotes pathogenic IL-17 T cell responses. The regulatory mechanisms in DCs that control these two cells types are not u...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1002099107

    authors: Murugaiyan G,Mittal A,Weiner HL

    更新日期:2010-06-22 00:00:00

  • Simultaneous, accurate measurement of the 3D position and orientation of single molecules.

    abstract::Recently, single molecule-based superresolution fluorescence microscopy has surpassed the diffraction limit to improve resolution to the order of 20 nm or better. These methods typically use image fitting that assumes an isotropic emission pattern from the single emitters as well as control of the emitter concentratio...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1216687109

    authors: Backlund MP,Lew MD,Backer AS,Sahl SJ,Grover G,Agrawal A,Piestun R,Moerner WE

    更新日期:2012-11-20 00:00:00

  • The CDX1-microRNA-215 axis regulates colorectal cancer stem cell differentiation.

    abstract::The transcription factor caudal-type homeobox 1 (CDX1) is a key regulator of differentiation in the normal colon and in colorectal cancer (CRC). CDX1 activates the expression of enterocyte genes, but it is not clear how the concomitant silencing of stem cell genes is achieved. MicroRNAs (miRNAs) are important mediator...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1503370112

    authors: Jones MF,Hara T,Francis P,Li XL,Bilke S,Zhu Y,Pineda M,Subramanian M,Bodmer WF,Lal A

    更新日期:2015-03-31 00:00:00

  • Estrogen differentially regulates neuropeptide gene expression in a sexually dimorphic olfactory pathway.

    abstract::The posterodorsal part of the medial nucleus of the amygdala (MeAp) receives its major sensory input from the accessory olfactory bulb and projects massively to the medial preoptic nucleus and other sexually dimorphic hypothalamic nuclei thought to play key roles in mediating steroid-sensitive reproductive functions. ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.86.12.4766

    authors: Simerly RB,Young BJ,Capozza MA,Swanson LW

    更新日期:1989-06-01 00:00:00

  • Quantitative dissection of hydrogen bond-mediated proton transfer in the ketosteroid isomerase active site.

    abstract::Hydrogen bond networks are key elements of protein structure and function but have been challenging to study within the complex protein environment. We have carried out in-depth interrogations of the proton transfer equilibrium within a hydrogen bond network formed to bound phenols in the active site of ketosteroid is...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1302191110

    authors: Sigala PA,Fafarman AT,Schwans JP,Fried SD,Fenn TD,Caaveiro JM,Pybus B,Ringe D,Petsko GA,Boxer SG,Herschlag D

    更新日期:2013-07-09 00:00:00

  • Involvement of Lyt-2 and L3T4 in activation of hapten-specific Lyt-2+ L3T4+ T-cell clones.

    abstract::The murine T-cell surface molecules Lyt-2 and L3T4 play a role in the activation of antigen-specific T cells. The currently accepted model for the function of these molecules proposes that Lyt-2 and L3T4 increase the overall avidity of the interaction between the T-cell antigen receptor and antigen in association with...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.83.8.2594

    authors: Fazekas de St Groth B,Gallagher PF,Miller JF

    更新日期:1986-04-01 00:00:00

  • Induction by lithium ion of multiplication of mouse mammary epithelium in culture.

    abstract::Lithium ion at concentrations between 2 and 20 mM simulated the stimulatory effects of insulin on the uptake of alpha-aminoisobutyric acid, synthesis of RNA and DNA, and cell multiplication in mouse mammary gland explants cultured in a chemically defined synthetic medium. Other monovalent cations were virtually ineffe...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.76.6.2823

    authors: Hori C,Oka T

    更新日期:1979-06-01 00:00:00

  • Coexistence of phycoerythrin and a chlorophyll a/b antenna in a marine prokaryote.

    abstract::Prochlorococcus marinus CCMP 1375, a ubiquitous and ecologically important marine prochlorophyte, was bound to possess functional genes coding for the alpha and beta subunits of a phycobiliprotein. The latter is similar to phycoerythrins (PE) from marine Synechococcus cyanobacteria and bind a phycourobilin-like pigmen...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.93.20.11126

    authors: Hess WR,Partensky F,van der Staay GW,Garcia-Fernandez JM,Börner T,Vaulot D

    更新日期:1996-10-01 00:00:00

  • Novel Epac fluorescent ligand reveals distinct Epac1 vs. Epac2 distribution and function in cardiomyocytes.

    abstract::Exchange proteins directly activated by cAMP (Epac1 and Epac2) have been recently recognized as key players in β-adrenergic-dependent cardiac arrhythmias. Whereas Epac1 overexpression can lead to cardiac hypertrophy and Epac2 activation can be arrhythmogenic, it is unknown whether distinct subcellular distribution of ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1416163112

    authors: Pereira L,Rehmann H,Lao DH,Erickson JR,Bossuyt J,Chen J,Bers DM

    更新日期:2015-03-31 00:00:00

  • Nuclear uptake of 1,25-dihydroxy[3H]cholecalciferol in dispersed fibroblasts cultured from normal human skin.

    abstract::Because of the relative inaccessibility of known calciferol target tissues (i.e., intestine and bone), we examined fibroblasts derived from normal human skin and grown in tissue culture as a means of evaluating the interaction of 1,25-dihydroxycholecalciferol [1,25(OH)2D3] and its effector system. When dispersed, inta...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.78.4.2562

    authors: Eil C,Marx SJ

    更新日期:1981-04-01 00:00:00

  • 3-Hydroxy-3-methylglutaryl CoA reductase inhibitors up-regulate transforming growth factor-beta signaling in cultured heart cells via inhibition of geranylgeranylation of RhoA GTPase.

    abstract::Transforming growth factor-beta (TGFbeta) signaling has been shown to play a role in cardiac development as well as in the pathogenesis of cardiovascular disease. Prior studies have suggested a relationship between cholesterol metabolism and TGFbeta signaling. Here we demonstrate that induction of the cholesterol meta...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.96.20.11525

    authors: Park HJ,Galper JB

    更新日期:1999-09-28 00:00:00

  • Construction and characterization of a reovirus double temperature-sensitive mutant.

    abstract::The infectious reovirus RNA system was used to construct a mutant with two temperature-sensitive (ts) lesions in genome segments M2 and S2, respectively. The double mutant is about 300 times more ts than either of its parents, which are about 1,500 and 170 times more ts than their wild-type parent reovirus ST3 strain ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.94.13.6826

    authors: Roner MR,Nepliouev I,Sherry B,Joklik WK

    更新日期:1997-06-24 00:00:00