Abstract:
OBJECTIVE:The purpose of this study was two-fold: (1) to monitor bacterial biofilm formation and bacteria-induced demineralization of dentine in situ by using electrochemical impedance spectrum (EIS); (2) to examine the relationship between EIS findings and changes in the chemical composition and ultrastructure of dentine during bacteria-induced demineralization. METHODS:In this study, dentine demineralization was induced by Streptococcusmutans (ATCC 25175) in the presence of sucrose in culture medium and was monitored using two EIS measurement systems (Type A with a working electrode and Type B without a working electrode). Scanning electron microscopy (SEM), field emission scanning electron microscopy (FESEM), energy dispersive X-ray (EDX) and X-ray diffraction (XRD) were employed to examine the morphology, element contents and crystallinity of hydroxyapatite (HAP) on the dentine surface. Transverse microradiography (TMR) was used to characterize the lesion depth and degree of mineral loss during demineralization. RESULTS:The resistance of the bulk dentine (R(d)) and the apparent resistance of dentine (R(a)) measured from the Type A and Type B EIS systems, respectively, decreased gradually with demineralization. The resistance of the biofilm formed on dentine surface was determined by fitting the EIS data with equivalent circuits. The presence of biofilm slightly increased R(a) of dentine before demineralization. However, the electrochemical behavior of biofilm did not affect the decreasing impedance of dentine with demineralization. The SEM, EDX, XRD and TMR results demonstrated that the surface and bulk dentine gradually became more porous due to the loss of minerals during demineralization, which in turn resulted in the decrease in R(d) and R(a) values obtained from EIS systems. CONCLUSIONS:This investigation highlighted EIS as a potential technique to monitor biofilm formation and bacterial-induced demineralization in situ.
journal_name
J Dentjournal_title
Journal of dentistryauthors
Xu Z,Neoh KG,Amaechi B,Kishen Adoi
10.1016/j.jdent.2009.09.013subject
Has Abstractpub_date
2010-02-01 00:00:00pages
138-48issue
2eissn
0300-5712issn
1879-176Xpii
S0300-5712(09)00230-9journal_volume
38pub_type
杂志文章abstract::In the present study Class II resin composite restorations were placed under in vivo and in vitro conditions. For the in vivo part. Class II preparations were cut in teeth destined for extraction for orthodontic reasons. The preparations were completed with the gingival margin in enamel and restored using six differen...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/0300-5712(93)90026-m
更新日期:1993-06-01 00:00:00
abstract:OBJECTIVES:To investigate repairs of direct restorations by a group of Dutch general dental practitioners (GDPs) and its consequences on longevity of restorations. METHODS:Data set was based on dental records of patients attending 11 general dental practices (24 Dentists) in the Netherlands. Patients that received Cla...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2017.06.002
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVES:The failure of the bond between acrylic resin denture base material and resin teeth remains a significant problem. This study evaluated the tensile bond strength of specimens produced by commonly employed tooth preparation and processing methods as used in dental laboratories. METHODS:Twenty-two experimenta...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/s0300-5712(98)00034-7
更新日期:1999-02-01 00:00:00
abstract:OBJECTIVES:Health condition of the gingival tissues contacting the surfaces of fixed prostheses is a result of multiple etiologic factors. The aim of the investigation discussed here was to evaluate the attachment and proliferation rate of cultured human epithelial cells on three commonly used restorative materials und...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2013.11.008
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVES:Traditional mechanical methods of retaining restorative materials have been replaced to a large extent by tooth conserving adhesive restorative techniques. Because adhesives have been evolving so rapidly for the last few years, the timing is right for evaluating the clinical status of present day adhesives. ...
journal_title:Journal of dentistry
pub_type: 杂志文章,评审
doi:10.1016/s0300-5712(96)00070-x
更新日期:1998-01-01 00:00:00
abstract:OBJECTIVES:Chlorhexidine (CHX) is a commonly used antiseptic mouthwash, used by dental practitioners and the public, due to its antimicrobial effects. The aim of this article was to provide a narrative review of current antimicrobial uses of CHX relevant to dentistry in the context of oral diseases, highlighting need f...
journal_title:Journal of dentistry
pub_type: 杂志文章,评审
doi:10.1016/j.jdent.2020.103497
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVES:This study evaluated the effect of propolis associated with Biosilicate on the bond strength (BS) and gelatinolytic activity at the adhesive/dentin interface. METHODS:Occlusal cavities were prepared in 320 human molars. Half of them were submitted to cariogenic challenge. All the teeth were separated into e...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2021.103585
更新日期:2021-01-16 00:00:00
abstract:OBJECTIVES:To validate a new questionnaire for evaluating the 'Oral aesthetic-related quality of life (OARQoL)' of prosthetically restored patients. 'OARQoL' assesses the impact of the self-perceived dental aesthetics on patients' well-being. METHODS:The 'Quality of Life associated with Dental Aesthetic Satisfaction (...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2015.08.010
更新日期:2015-11-01 00:00:00
abstract::Replacement of pin-retained amalgam restorations is complicated by the loss of prime pin sites and remaining pin sites posing anatomical problems. Self-threading dentine pins can be removed with an ultrasonic handpiece, and the pin site reused with a large pin. Retention of replacement pins is similar to original pins...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/0300-5712(93)90109-4
更新日期:1993-10-01 00:00:00
abstract:OBJECTIVE:The present study tested the central hypothesis that selective demineralisation of dentine extrafibrillar minerals by lowering the phosphoric acid concentration improves the quality of the resin-dentine interface. METHODS:Dentine surfaces were etched with different concentrations of phosphoric acid (1, 5, 10...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2016.07.008
更新日期:2016-09-01 00:00:00
abstract:OBJECTIVES:To elucidate the impact of surface properties and the release of fluoride from different glass ionomer cements on biofilm formation. METHODS:Standardized specimens manufactured from various classes of glass ionomer cements (GICs), a resin-based composite (RBC), and human enamel were subjected to surface ana...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2017.02.005
更新日期:2017-05-01 00:00:00
abstract:OBJECTIVES:Sleep bruxism (SB), characterized by repetitive jaw-muscle activity during sleep, is often suggested as a cause of temporomandibular disorders (TMD), orofacial pain, and headache. This study aimed to challenge the relationship between jaw-muscle electromyographic (EMG) activity during sleep and jaw muscle sy...
journal_title:Journal of dentistry
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.jdent.2019.01.013
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVE:This study evaluated whether Quantitative Light-induced Fluorescence-Digital (QLF-D) can detect the levels of cariogenicity of dental microcosm biofilms by assessing the red fluorescence intensity. METHODS:Dental microcosm biofilms were initiated from human saliva on bovine enamel discs. Biofilms with variou...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2013.08.021
更新日期:2013-12-01 00:00:00
abstract:OBJECTIVES:To investigate the effectiveness of an indicator gel in revealing exposed dentine. METHODS:Teeth prepared into dentine and then stained with the indicator gel were sectioned for histological examination. RESULTS:The indicator gel was found to stain dentine and thin layers of enamel. CONCLUSION:The gel is ...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/0300-5712(94)00010-d
更新日期:1995-12-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to compare the behavior of two new, low-shrinkage hybrid composites (Aelite LS and Inten-S) with a microfilled (Heliomolar) and a hybrid composite (Filtek Z250), in terms of polymerization contraction stress and microleakage. METHODS:Maximum contraction stress after 10 min was recor...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2004.01.014
更新日期:2004-07-01 00:00:00
abstract:OBJECTIVES:This study concerned the temperature and linear dimensional change of heat-cured acrylic resin in the two-stage processing technique for complete dentures. METHODS:Thermocouples were incorporated in the acrylic resin for recording temperatures. Measurements between reference marks were made by a high-resolu...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/0300-5712(95)91190-x
更新日期:1995-08-01 00:00:00
abstract:OBJECTIVES:To evaluate the influence of tooth bleaching on the opalescence properties of enamel. METHODS:Color of 38 specimens was determined by colorimetric analysis before and after bleaching in the reflectance and transmittance modes. The results were described as CIELab color coordinates. The Opalescent Parameter ...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2012.01.011
更新日期:2012-07-01 00:00:00
abstract:OBJECTIVES:The purpose of this study was to evaluate the in vitro effectiveness of a new bioglass-containing and two commercial desensitising toothpastes on dentinal tubule occlusion after citric acid challenge or artificial saliva (AS) immersion. METHODS:One hundred dentin discs from human third molars were used. Spe...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2010.01.007
更新日期:2010-05-01 00:00:00
abstract:OBJECTIVES:To investigate differentially expressed salivary peptides in the development of early childhood caries (ECC) in 3-4 year-old children. MATERIALS AND METHODS:Eighty-two caries-free children at baseline were followed-up for 1year, during which period 15 of them had developed ECC (Group C), whilst another 15 c...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2017.04.006
更新日期:2017-06-01 00:00:00
abstract::The effect of various surface treatments on the shear bond strength of composite resin to a glass-ionomer cement has been studied. Acid etching with phosphoric acid and polyacrylic acid, roughening by way of grinding or air drying, the use of a dentine bonding system and a silane coupling agent were the variables. Aci...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/0300-5712(89)90004-3
更新日期:1989-02-01 00:00:00
abstract:OBJECTIVES:To make an inventory of clinical studies on veneer restorations (VRs) using a meta-analysis review procedure and to aggregate an overall survival result for four types of VRs. METHODS:From the dental literature published from 1983 to November 1996, papers were selected using an in- and exclusion protocol in...
journal_title:Journal of dentistry
pub_type: 杂志文章,meta分析
doi:10.1016/s0300-5712(97)00023-7
更新日期:1998-05-01 00:00:00
abstract:OBJECTIVES:To assess the in situ color stability, surface and the tooth/restoration interface degradation of a silorane-based composite (P90, 3M ESPE) after accelerated artificial ageing (AAA), in comparison with other dimethacrylate monomer-based composites (Z250/Z350, 3M ESPE and Esthet-X, Dentsply). METHODS:Class V...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2011.03.003
更新日期:2011-07-01 00:00:00
abstract:OBJECTIVES:This study was performed to determine the compositions of commercial temporary restorative resins and to evaluate the leachability of plasticizer and residual monomer from them. METHODS:The chemicals in four commercial temporary restorative resins (Dura Seal, Fit Seal, Plast Seal Quick, and Poly Seal) were ...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2003.12.004
更新日期:2004-05-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to investigate the mechanical properties of five types of fibre-reinforced composite (FRC) posts and compare them with traditional metal post. METHODS:Five FRC posts and a metallic post having different geometry and type of fibre (glass, carbon or quartz fibre) were loaded to failu...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2012.08.003
更新日期:2012-11-01 00:00:00
abstract:OBJECTIVES:To evaluate the effect of the addition of epigallocatechin-3-gallate (EGCG) on the antibacterial and physical properties of glass ionomer cement (GIC). METHODS:A conventional GIC, Fuji IX, was used as a control. EGCG was incorporated into GIC at 0.1% (w/w) and used as the experimental group. Chlorhexidine (...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2013.07.014
更新日期:2013-10-01 00:00:00
abstract:OBJECTIVES:The purpose of the study was to evaluate gloss and color changes of resin composites after exposure to different bleaching agents. METHODS:Products used, were: Nite White ACP (10% carbamide peroxide gel), Crest Classic and Supreme Whitestrip (6.5% and 14% hydrogen peroxide strips, accordingly). A hybrid (He...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2010.06.006
更新日期:2010-01-01 00:00:00
abstract:OBJECTIVES:The primary cleaning action of powered toothbrushes is the motion of the bristles in contact with the tooth surfaces. The aim of this study was to use scanning laser vibrometry to determine the effects of load and toothpaste on the bristle vibration characteristics of four powered toothbrushes (Oral-B Sonic ...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2006.11.006
更新日期:2007-04-01 00:00:00
abstract:OBJECTIVES:this study aimed to synthesize AgNPs from green tea (GT) extract, forming GT-AgNPs, and to coat their surfaces with silica, resulting in light-colored Ag@SiO2 nanoparticles. MATERIALS AND METHODS:particles were characterized and tested for minimal inhibitory concentration (MIC), biofilm formation against St...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/j.jdent.2020.103327
更新日期:2020-05-01 00:00:00
abstract:OBJECTIVES:A meta-analysis was conducted to assess the relationship between parental age and the occurrence of non-syndromic oral clefts. The questions addressed if younger or older mothers and fathers have an increased risk of having a child with non-syndromic oral clefts. DATA:Data from cohort studies, case-control,...
journal_title:Journal of dentistry
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.jdent.2011.10.002
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVE:This study compared the effect of water sorption on the extent of marginal gap reduction in a compomer (Dyract AP, Dentsply) and a composite (Spectrum, Dentsply) over a 12-week storage period. MATERIALS AND METHODS:Artificial gaps were created in 40 borosilicate glass cylinders, each 5mm deep and with an int...
journal_title:Journal of dentistry
pub_type: 杂志文章
doi:10.1016/s0300-5712(01)00053-7
更新日期:2002-01-01 00:00:00