Abstract：
OBJECTIVE:This study evaluated whether Quantitative Light-induced Fluorescence-Digital (QLF-D) can detect the levels of cariogenicity of dental microcosm biofilms by assessing the red fluorescence intensity. METHODS:Dental microcosm biofilms were initiated from human saliva on bovine enamel discs. Biofilms with various levels of cariogenicity were then grown in artificial saliva supplemented with sucrose at different concentrations (0.05%, 0.1%, 0.2%, and 0.5%) in 24-well microplates. After 10 days, fluorescence images of the biofilms were captured by the QLF-D to analyse the red fluorescence intensity, which was quantified as the red/green ratio (R/G value). The supernatant pH was also measured, as well as the total and aciduric bacteria counts of the collected biofilms. Mineral loss in enamel was also evaluated by calculating the percentage of surface microhardness changes (%SHC). RESULTS:The R/G values of the biofilms differed significantly with the sucrose concentration (p<0.0001), increasing consistently as the sucrose concentration increased from 0.05% (=0.91) to 0.5% (=2.56). Strong correlation was identified between the R/G value and the number of aciduric bacteria (r=0.83, p<0.0001), supernatant pH (r=-0.95, p<0.0001), and %SHC (r=0.90, p<0.0001). CONCLUSIONS:The red fluorescence as observed by the QLF-D was correlated with the cariogenic properties of dental microcosm biofilms in vitro, which indicates that this device can be used to detect the levels of cariogenicity of a dental biofilm. CLINICAL SIGNIFICANCE:The QLF-D is able to assess the cariogenic levels of dental plaque based on the intensity of red fluorescence.
journal_name
J Dentjournal_title
Journal of dentistryauthors
Lee ES,Kang SM,Ko HY,Kwon HK,Kim BIdoi
10.1016/j.jdent.2013.08.021subject
Has Abstractpub_date
2013-12-01 00:00:00pages
1264-70issue
12eissn
0300-5712issn
1879-176Xpii
S0300-5712(13)00225-Xjournal_volume
41pub_type
杂志文章abstract：OBJECTIVES:The objective of the study was to determine the perceptibility and acceptability thresholds for dental ceramics using CIEDE2000 (ΔE(00)) and CIELAB (ΔE(ab)(*)) color difference formulas and a novel TSK Fuzzy Approximation. METHODS:A 13-observer panel performed independent observations of perceptibility and ...
journal_title：Journal of dentistry
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更新日期：2010-01-01 00:00:00
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pub_type： 杂志文章
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abstract：OBJECTIVES:To assess sustainability of shortened dental arches (SDA) by determining time to 'first restorative intervention' of teeth and time to 'tooth loss' and comparing these outcomes with complete dental arches (CDA) and SDA plus removable dental prostheses (RDP). METHODS:Data (follow-up time ranged from 27.4 (SD...
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更新日期：2013-08-01 00:00:00
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journal_title：Journal of dentistry
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doi：10.1016/j.jdent.2013.07.014
更新日期：2013-10-01 00:00:00
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更新日期：2002-09-01 00:00:00
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doi：10.1016/j.jdent.2009.06.014
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更新日期：2007-04-01 00:00:00
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pub_type： 杂志文章
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更新日期：2015-02-01 00:00:00
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journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/s0300-5712(01)00026-4
更新日期：2001-07-01 00:00:00
abstract：:A significant correlation was found between the number of mutans streptococci in saliva and the proportional distribution of these microorganisms in pooled plaque from the margins of all restorations. At high salivary numbers there was a tendency towards a higher porportion of mutans streptococci in plaque from the ma...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/0300-5712(92)90130-5
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doi：10.1016/j.jdent.2020.103354
更新日期：2020-08-01 00:00:00
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journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2012.09.001
更新日期：2012-12-01 00:00:00
abstract：OBJECTIVES:The purpose of this study was to evaluate the in vitro effectiveness of a new bioglass-containing and two commercial desensitising toothpastes on dentinal tubule occlusion after citric acid challenge or artificial saliva (AS) immersion. METHODS:One hundred dentin discs from human third molars were used. Spe...
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doi：10.1016/j.jdent.2010.01.007
更新日期：2010-05-01 00:00:00
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更新日期：2020-05-01 00:00:00
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journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2013.12.001
更新日期：2014-03-01 00:00:00
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journal_title：Journal of dentistry
pub_type： 临床试验,杂志文章,随机对照试验
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journal_title：Journal of dentistry
pub_type： 杂志文章,meta分析,评审
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更新日期：2016-06-01 00:00:00
abstract：OBJECTIVE:The purpose of this paper is to review current knowledge and technologies for tooth remineralisation. DATA SOURCES:The literature was searched using the "Scopus" and "Web of Knowledge" database from the year 1971, with principal key words of *miner*, teeth and enamel. Language was restricted to English. Orig...
journal_title：Journal of dentistry
pub_type： 杂志文章,评审
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更新日期：2014-06-01 00:00:00
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journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/s0300-5712(02)00018-0
更新日期：2002-07-01 00:00:00
abstract：:In the present study Class II resin composite restorations were placed under in vivo and in vitro conditions. For the in vivo part. Class II preparations were cut in teeth destined for extraction for orthodontic reasons. The preparations were completed with the gingival margin in enamel and restored using six differen...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/0300-5712(93)90026-m
更新日期：1993-06-01 00:00:00
abstract：OBJECTIVES:The purpose of this study was to evaluate the capacity of six contemporary self-etch primers/adhesives to demineralize ground enamel by means of ultrastructural analysis of the etching surface under SEM and by spectroscopic measurement of the percentage of calcium and phosphate ions dissolved. METHODS:Seven...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2006.11.003
更新日期：2007-05-01 00:00:00
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journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2013.05.005
更新日期：2013-07-01 00:00:00
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journal_title：Journal of dentistry
pub_type： 杂志文章,评审
doi：10.1016/j.jdent.2013.08.012
更新日期：2013-11-01 00:00:00
abstract：OBJECTIVES:This study aimed to evaluate posterior restorations placed in young adults, investigating the association between social determinants experienced during the life course and the quality of tooth fillings. METHODS:A representative sample (n=720) of all 5914 individuals who were born in Pelotas in 1982 was pro...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2013.02.010
更新日期：2013-11-01 00:00:00
abstract：OBJECTIVES:The aim of this in vitro study was to evaluate the penetration of an infiltrant and a sealant, when applied as recommended, into fissure caries lesions. METHODS:The fissure systems of extracted human teeth were classified according to the international caries detection and assessment system (ICDAS, codes: 0...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2014.01.006
更新日期：2014-04-01 00:00:00
abstract：PURPOSE:Evaluate the dentine bond strength using different methods of application of opaque resins to mask dentine discoloration. MATERIALS AND METHODS:Dentine was exposed on 21 extracted molars which were assigned to 3 groups: G1: immediate dentine sealing (IDS). G2: IDS+opaque. G3: IDS with adhesive resin mixed with...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2011.07.005
更新日期：2011-10-01 00:00:00
abstract：OBJECTIVES:The study evaluated the antibacterial effect of VIOlight (VL) Personal Travel Toothbrush Sanitizer on biofilms after toothbrush exposure to human saliva compared to Listerine Antiseptic (LA), 3% hydrogen peroxide (3%HP) and water. METHODS:Twenty toothbrush heads (n=5/Gp) were immersed in saliva and to allow...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2009.08.011
更新日期：2010-08-01 00:00:00
abstract：OBJECTIVES:To evaluate the effect of application method on immediate and 6-month resin-dentin microtensile bond strength (microTBS) and nanoleakage (NL) of three one-step self-etch adhesives systems (Clearfil S(3) Bond (S3), Xeno III (XE) and Adper Prompt L-Pop (AD)). METHODS:The oclusal enamel of 30 human molar was r...
journal_title：Journal of dentistry
pub_type： 杂志文章
doi：10.1016/j.jdent.2008.09.010
更新日期：2009-01-01 00:00:00
