Biofilm formation and release of fluoride from dental restorative materials in relation to their surface properties.

Abstract:

OBJECTIVES:To elucidate the impact of surface properties and the release of fluoride from different glass ionomer cements on biofilm formation. METHODS:Standardized specimens manufactured from various classes of glass ionomer cements (GICs), a resin-based composite (RBC), and human enamel were subjected to surface analyses. Subsequent to simulation of salivary pellicle formation, Streptococcus mutans biofilm formation was initiated using a drip flow reactor for 48h and 96h. Biofilms were characterized by determining viable bacterial biomass and 3D biofilm architecture using SEM and CLSM; the release of fluoride from the specimens was measured using the ion selective micro method in dependence on various experimental conditions (incubation with sterile broth/bacteria/acid). RESULTS:Surface properties and biofilm formation correlated poorly, while the release of fluoride correlated well with viable streptococcal biomass and SEM/CLSM analyses. For all investigated materials, biofilm formation was lower than on enamel. The release of fluoride showed a significant dependency on the experimental conditions applied; the presence of biofilms reduced fluoride release in comparison to sterile incubation conditions. CONCLUSIONS:Within the limitations of a laboratory study, the results suggest that biofilm formation on GICs cannot be easily predicted as a function of substratum surface parameters. The release of fluoride from glass ionomer cements contributes to control biofilm formation particularly in its early phases. CLINICAL SIGNIFICANCE:Glass ionomer cements can actively control microbial biofilm formation, while biofilms modulate the release of fluoride from GIC materials.

journal_name

J Dent

journal_title

Journal of dentistry

authors

Hahnel S,Ionescu AC,Cazzaniga G,Ottobelli M,Brambilla E

doi

10.1016/j.jdent.2017.02.005

subject

Has Abstract

pub_date

2017-05-01 00:00:00

pages

14-24

eissn

0300-5712

issn

1879-176X

pii

S0300-5712(17)30033-7

journal_volume

60

pub_type

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