Abstract：
:In the present study, cellular tropism and relative transduction efficiency of AAV2/6, AAV2/8 and AAV2/9 vectors have been tested for the cornea using primary cultures of human corneal fibroblasts. The AAV6, AAV8 and AAV9 serotypes having AAV2 ITR plasmid encoding for alkaline phosphatase (AP) gene were generated by transfecting HEK293 cell line with pHelper, pARAP4 and pRep/Cap plasmids. Primary cultures of human corneal fibroblasts were exposed to AAV infectious particles at two different doses (1 x 10(5) and 2 x 10(5) MOI). Cytochemistry and enzyme assays were used to measure delivered transgene expression in samples collected at 4 and 30 h after AAV infection by counting AP-stained cells or quantifying AP enzyme activity. Cellular toxicity of AAVs was evaluated with TUNEL and trypan blue assays. All three AAV serotypes transduced human corneal fibroblasts. The order of transduction efficiency was AAV2/6>AAV2/9>AAV2/8. The transduction efficiency of AAV2/6 was 30-50-fold higher (p < 0.001) for the human corneal fibroblasts compared to the AAV2/8 or AAV2/9 at two tested doses. The level of transgene expression at 4h was considerably low compared to 30 h suggesting that the transgene delivery did not reach its peak at 4h. Cultures exposed to any of the three AAV serotypes showed more than 97% cellular viability and less than 5 TUNEL positive cells suggesting that tested AAV serotypes do not induce significant cell death and are safe for corneal gene therapy.
journal_name
Brain Res Bulljournal_title
Brain research bulletinauthors
Sharma A,Ghosh A,Hansen ET,Newman JM,Mohan RRdoi
10.1016/j.brainresbull.2009.07.005subject
Has Abstractpub_date
2010-02-15 00:00:00pages
273-8issue
2-3eissn
0361-9230issn
1873-2747pii
S0361-9230(09)00213-5journal_volume
81pub_type
杂志文章abstract：:The elevated plus-maze is an animal model used to study anxiety. In a second session, rats show a reduction in the exploratory behavior even when the two sessions are separated by intervals as large as 7 days. The aim of the present study was to investigate whether the reduction in the exploratory behavior is maintain...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2010.01.007
更新日期：2010-04-05 00:00:00
abstract：:Intractable scratching is the characteristic of chronic itch, which represents a great challenge in clinical practice. However, the mechanism underlying chronic itch development is largely unknown. In the present study, we investigated the role of NMDA receptor in acute itch and in development of chronic itch. A mouse...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2020.02.011
更新日期：2020-05-01 00:00:00
abstract：:Intracerebroventricular (IVT) administration of renin (R) to conscious male hydrated rats induces an increase in sodium excretion. The involvement of brain dopaminergic neurons in the renal action of IVT-R was investigated. Renin-induced natriuretic action was prevented by domperidone and by inhibition of tyrosine hyd...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(96)00032-9
更新日期：1996-01-01 00:00:00
abstract：:Methoxetamine (MXE) is a novel psychoactive compound (NPS) that emerged in 2010 as a substitute for the dissociative anaesthetic ketamine. MXE has a reputation of carrying a lower risk of harm than ketamine, however a number of deaths have been reported. Currently very little is known about the psychopharmacological e...
journal_title：Brain research bulletin
pub_type： 杂志文章,评审
doi：10.1016/j.brainresbull.2016.05.002
更新日期：2016-09-01 00:00:00
abstract：BACKGROUND:Nitric oxide (NO) is a gaseous molecule that modulates several physiological processes, including signal transmission in the central nervous system. There is evidence supporting NO as a major neurotransmitter involved in motor and emotion/behavior control. We investigated the distribution and morphology of n...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2016.04.003
更新日期：2016-06-01 00:00:00
abstract：:The conditions under which amyloid is toxic to primary rat hippocampal neurons were investigated. Synthetic A beta (1-42) peptide elicited neurotoxic activity following "aging" for 7 to 14 days at 37 degrees C in Modified Eagles Media. Neurotoxicity included decreases in neurite length, cell number, and a loss in 3-[4...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(95)02034-x
更新日期：1995-01-01 00:00:00
abstract：:Gamma-aminobutyric acid (GABA) is an important inhibitory neurotransmitter in the mature brain. At an early developmental period, it acts in an excitatory manner that influences many processes of proliferation, migration, and differentiation of the neurons. Previous evidence indicated that manipulation of the GABAergi...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2021.01.006
更新日期：2021-01-10 00:00:00
abstract：:Sleepiness and driving is a dangerous combination that causes thousands of crashes each year resulting in injury and death. In the last few years, driving simulators have been used to study the performance decrements associated with drowsiness. We correlated performances of a driving simulation task in healthy volunte...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2003.12.015
更新日期：2004-06-30 00:00:00
abstract：:Developmental malformations of cortex have been shown to co-occur with language, learning, and other cognitive deficits in humans. Rodent models have repeatedly shown that animals with such developmental malformations have deficits related to auditory processing and learning. More specifically, freeze-lesion induced m...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2006.11.005
更新日期：2007-03-15 00:00:00
abstract：:The effects of a low protein diet during gestation, lactation, and after weaning on the anatomical development of a subcortical nucleate formation, the neostriatum, and a reticulate formation, the diagonal band of Broca, were studied. At 10, 30, and 90 days the volume of the neostriatum was decreased in the experiment...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(78)90073-4
更新日期：1978-09-01 00:00:00
abstract：OBJECTIVE:Cerebral ischemic stroke (IS) threatens the daily life of individuals, with high mortality. Emerging studies have deciphered the independent roles of the long non-coding RNA growth-arrest-specific transcript 5 (GAS5), microRNA (miR)-455-5p and phosphatase and tensin homolog (PTEN) in IS, but the understanding...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2020.12.002
更新日期：2021-02-01 00:00:00
abstract：:The present study examined whether the induction of scent marking behavior and grooming of the flank gland region by injection of arginine-vasopressin (AVP) or oxytocin (OXY) into the anterior hypothalamus-medial preoptic area (AH-MPOA) required the presence of the flank glands. The flank glands were surgically remove...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(86)90242-x
更新日期：1986-09-01 00:00:00
abstract：:The microdialysis method was utilized to study the effects of N omega-nitro-L-arginine (L-NNA) and N omega-nitro-D-arginine (D-NNA), an inhibitor of nitric oxide (NO) synthesis from L-arginine and its D-enantiomer, on spontaneous and N-methyl-D-aspartate (NMDA)-evoked overflow of striatal extracellular dopamine (DA) i...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(94)90021-3
更新日期：1994-01-01 00:00:00
abstract：:The central GABAergic system is associated with normal blood pressure regulation, but the role of GABA receptors in genetic hypertension remains unclear. This study was conducted to investigate GABAA receptor binding in several brain regions of spontaneously hypertensive (SHR) rats during development of hypertension. ...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(94)00164-v
更新日期：1995-01-01 00:00:00
abstract：:The objective of this study was to determine the organization of coactivation in the arm and shoulder muscles. Normal human subjects made alternate movements of a joint in the horizontal plane, either adduction-abduction of the second finger and shoulder, ulnar-radial deviation of the wrist, or extension-flexion of th...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(02)00952-8
更新日期：2003-02-15 00:00:00
abstract：:Extracellular matrix (ECM) molecules in the central nervous system form highly organized ECM structures around cell somata, axon initial segments, and synapses and play prominent roles in early development by guiding cell migration, neurite outgrowth and synaptogenesis, and by regulating closure of the critical period...
journal_title：Brain research bulletin
pub_type： 杂志文章,评审
doi：10.1016/j.brainresbull.2017.03.003
更新日期：2018-01-01 00:00:00
abstract：:We quantitated the activity of ornithine decarboxylase (ODC) in homogenates and subcellular fractions of inner ear tissues from the rat and guinea pig and demonstrate inhibition of cochlear ODC by the aminoglycoside neomycin. Subcellular fractionation showed the enzyme associated with the post-mitochondrial supernatan...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(87)90056-6
更新日期：1987-12-01 00:00:00
abstract：:This study is a summary of investigations in the last decade with several collaborators on representatives of different vertebrate stocks. The results suggest that in the main vertebrate stocks (Agnathi, Chondrichthyes, Actinopterygii, Sarcopterygii-Amniotes), which had their parallel brain evolutions from the laminar...
journal_title：Brain research bulletin
pub_type： 杂志文章,评审
doi：10.1016/s0361-9230(01)00713-4
更新日期：2002-02-01 00:00:00
abstract：:The localization of CGRP mRNA in neurons of the rat brain and spinal cord was assessed by in situ hybridization histochemistry (ISH) using a radiolabeled synthetic 57-mer oligodeoxynucleotide probe complementary to the rat prepro CGRP mRNA. Results were compared with previously published findings of CGRP-immunoreactiv...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(94)00201-b
更新日期：1995-01-01 00:00:00
abstract：:Spinal astrocytes have emerged as important mechanistic contributors to pathological and chronic pain. Recently, we have demonstrated that injection of diluted bee venom (DBV) into the Zusanli (ST36) acupoint produces a potent anti-nociceptive effect via the activation of spinal alpha-2 adrenoceptors. However, it is u...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2011.08.012
更新日期：2011-11-25 00:00:00
abstract：:Binary mixtures of acid with salt were observed to produce enhancing effects on oral chemoreceptor responses by recording the activity of the whole glossopharyngeal nerve of the clawed toad (Xenopus laevis). The mixtures of HCl with various inorganic salts elicited responses larger than the sum of the responses to ind...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(96)00326-7
更新日期：1997-01-01 00:00:00
abstract：:Adaptation of the frog, Rana temporaria, to a white background for 12 hr has resulted in an intense formaldehyde-induced fluorescence (FIF) in the neurons of the preoptic recess organ (PRO), paraventricular organ (PVO), nucleus infundibularis dorsalis (NID) and their basal processes permitting visualization of the PRO...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(82)90184-8
更新日期：1982-07-01 00:00:00
abstract：:The serotonin(2A) (5HT(2A)) receptors have been shown to play an important role in several psychiatric disorders, including depression, schizophrenia, and alcoholism. This immunohistochemical study examined the cellular localization of 5HT(2A) receptors in various rat brain structures (olfactory, striatum, cortex, hip...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(99)00278-6
更新日期：2000-04-01 00:00:00
abstract：:The thiol reagent cysteamine was administered to adult male rats with the aim of investigating its effect on different neural and pineal components. As expected, immunoreactive somatostatin decreased in the median eminence (ME) (p less than 0.05) and gastric antrum (p less than 0.05) after cysteamine; however, no sign...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(86)90051-1
更新日期：1986-03-01 00:00:00
abstract：:Fractal nature of the human sleep EEG was revealed recently. In the literature there are some attempts to relate fractal features to spectral properties. However, a comprehensive assessment of the relationship between fractal and power spectral measures is still missing. Therefore, in the present study we investigated...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2010.12.005
更新日期：2011-04-05 00:00:00
abstract：:Prenatal cocaine exposure increases mu-opioid receptor binding in dopaminergic terminal areas and enhances behavioral responsiveness to mu-opioid agonists. We investigated the influence of early postnatal cocaine treatment on in vitro mu- and delta-opioid receptor activation in male and female weanling rats. Pups rece...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(00)00323-3
更新日期：2000-09-15 00:00:00
abstract：:The present study was conducted to evaluate plasma levels of monoamine neurotransmitter metabolites in spontaneous recurrences of methamphetamine psychosis (i.e., flashbacks). The subjects were 50 physically healthy females comprised of 25 who experienced flashbacks (flashbackers), 18 who did not experienced methamphe...
journal_title：Brain research bulletin
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0361-9230(96)00340-1
更新日期：1997-01-01 00:00:00
abstract：:The effects of streptozotocin-induced (STZ) diabetes on the release of gonadotropins was studied in female rats. In the first experiment, rats were ovariectomized and 2 days later were injected with STZ. Three weeks later rats were treated with estrogen and progesterone and blood samples were taken via intraatrial can...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(93)90207-r
更新日期：1993-01-01 00:00:00
abstract：:We recently reported that human recombinant interleukin-6 (hrIL-6) microinjected into the preoptic area (POA) of guinea pigs induced fever at high doses, suggesting that IL-6 may be another endogenous pyrogen. This study was undertaken to determine whether hrIL-6 affects the single-unit activity of thermosensitive and...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(92)90005-i
更新日期：1992-07-01 00:00:00
abstract：:To elucidate the role of the medial basal hypothalamus (MBH) in stress-induced prolactin (Prl) release, adult male rats which had received monosodium glutamate (MSG 4 mg/g) on alternate days for the first 10 days of life were subjected to ether stress for 2 min. Blood samples were drawn at 0, 15, 30 and 60 min after e...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(83)90068-0
更新日期：1983-01-01 00:00:00
