Abstract:
:Salmonella enterica, a gram-negative pathogen, causes a spectrum of human infections including enterocolitis and typhoid fever. We previously showed that Salmonella flagellin played a role in suppressing intestinal mucosal inflammation in a murine model of acute enterocolitis. In this study, we examined the role of flagellin in the typhoid-like systemic murine Salmonella infection by measuring bacterial proliferation, inflammation, leukocyte recruitment, and cellular apoptosis in Peyer's patches (PPs), mesenteric lymph node (MLN), and spleen. We found that relative to an isogenic wild-type (WT) strain, aflagellate Salmonella exhibited increased proliferation at 4 days postinfection in PPs and MLN but not spleen. The aflagellate mutant also elicited increased local and systemic secretion of inflammatory cytokines such as interleukin-1beta, gamma interferon, and tumor necrosis factor alpha and enhanced surface expression of ICAM-1 on macrophages and dendritic cells (DCs). Furthermore, the recruitment of macrophages and DCs in PPs and MLN, but not spleen, was enhanced upon infection with aflagellate Salmonella. The relative differences between WT and aflagellate Salmonella were highly attenuated in Toll-like receptor 5-deficient (TLR5(-/-)) mice, indicating involvement of TLR5-dependent signaling. Interestingly, infection with the aflagellate mutant also resulted in decreased levels of T-cell apoptosis in PPs relative to infection with WT Salmonella. We postulate that the initial lack of detection of the aflagellate mutant in the mucosa permits increased proliferation within the host and enhances inflammatory signaling in nonepithelial cell types, which subsequently promotes leukocyte recruitment. In contrast, lack of difference in any disease parameter measured in the spleen likely reflects that Salmonella expression of flagellin is downregulated in this organ. Thus, the characteristic inflammatory pathology of Salmonella infection occurs only in PPs and to a lesser extent in MLN during the initial phases of infection and these early responses are dependent on TLR5.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Fournier B,Williams IR,Gewirtz AT,Neish ASdoi
10.1128/IAI.00656-09subject
Has Abstractpub_date
2009-09-01 00:00:00pages
4121-9issue
9eissn
0019-9567issn
1098-5522pii
IAI.00656-09journal_volume
77pub_type
杂志文章abstract::The adipocytokine leptin links nutritional status to immune function. Leptin signaling protects from amebiasis, but the molecular mechanism is not understood. We developed an in vitro model of ameba-host cell interaction to test the hypothesis that leptin prevents ameba-induced apoptosis in host epithelial cells. We d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06140-11
更新日期:2012-05-01 00:00:00
abstract::Antigen-presenting cells (APCs) are key players in the induction and regulation of immune responses. In Plasmodium falciparum malaria, determination of which cells and pathways are activated in the network of APCs remains elusive. We therefore investigated the effects of a controlled human malaria infection in healthy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00473-15
更新日期:2015-09-01 00:00:00
abstract::Immunoglobulin binding proteins are one of several pathogenicity factors which have been associated with invasive disease caused by group A streptococci. The surface-bound M and M-like proteins of Streptococcus pyogenes are the most characterized of these immunoglobulin binding proteins, and in most cases they bind on...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.8.4851-4857.2001
更新日期:2001-08-01 00:00:00
abstract::Lipopolysaccharide (LPS) structure impacts the bactericidal action of cationic peptides, such as polymyxin B (PMB), and sensitivity to killing by normal human serum (NHS). Cultivation of different subspecies strains of Yersinia pestis isolated from unrelated geographic origins at various temperatures (mammals, 37 degr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.11.7324-7331.2005
更新日期:2005-11-01 00:00:00
abstract::The protective immunity conferred by T-cell subsets against infection with Treponema pallidum subsp. pertenue was studied. We demonstrated that hamster T cells can be separated into two subsets by monoclonal antibody (MAb) GK 1.5 (anti-L3T4) and MAb 38. Eighty-five percent of hamster thymocytes were L3T4+ and 87% were...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.2.529-536.1991
更新日期:1991-02-01 00:00:00
abstract::Potassium thiocyanate extracts of a virulent Pasteurella multocida 3:A rabbit isolate were prepared and used as a vaccine in rabbits. The extract contained protein, carbohydrate, hyaluronic acid, lipopolysaccharide, DNA, and RNA. The protein and lipopolysaccharide profiles of the extract were similar to those of the P...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.12.2967-2976.1987
更新日期:1987-12-01 00:00:00
abstract::Borrelia burgdorferi elicits a potent cytokine response through activation of multiple signaling receptors on innate immune cells. Spirochetal lipoproteins initiate expression of NF-κB-dependent cytokines primarily via TLR2, whereas type I interferon (IFN) production is induced through the endosomal receptors TLR7 and...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01617-14
更新日期:2014-06-01 00:00:00
abstract::Avian pathogenic Escherichia coli (APEC) cause extraintestinal disease in avian species via respiratory tract infection. Virulence factors associated with APEC include type 1 and P fimbriae, curli, aerobactin, lipopolysaccharide (LPS), K1 capsular antigen, temperature-sensitive hemagglutinin (Tsh), and an uncharacteri...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.1.494-503.2003
更新日期:2003-01-01 00:00:00
abstract::We have determined the nucleotide sequence of the 1.4-kilobase DNA fragment containing the plasminogen activator gene (pla) of Yersinia pestis, which determines both plasminogen activator and coagulase activities of the species. The sequence revealed the presence of a 936-base-pair open reading frame that constitutes ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.5.1517-1523.1989
更新日期:1989-05-01 00:00:00
abstract::The recent finding that high numbers of strict anaerobes are present in the respiratory tract of cystic fibrosis (CF) patients has drawn attention to the pathogenic contribution of the CF microbiome to airway disease. In this study, we investigated the specific interactions of the most dominant bacterial CF pathogen, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02234-14
更新日期:2015-01-01 00:00:00
abstract::Tularemia is caused by the Gram-negative bacterial pathogen Francisella tularensis Infection of macrophages and their subsequent death are believed to play important roles in the progression of disease. Because complement is a particularly effective opsonin for Francisella, we asked whether complement-dependent uptake...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00424-17
更新日期:2017-09-20 00:00:00
abstract::Antibiotics suppressed development of aster yellows (AY) disease symptoms in plants of china aster [Callistephus chinensis (L.) Nees.] and annual chrysanthemum (Chrysanthemum carinatum, Schousb.). When inoculated chrysanthemum plants were treated by any of several techniques with tetracycline antibiotics or chloramphe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.2.201-208.1970
更新日期:1970-08-01 00:00:00
abstract::Type 1 fimbrial phase-locked mutants of uropathogenic Escherichia coli cystitis isolate F11 were used to assess the role of the invertible element during urinary tract infection. Compared to the wild type, the phase-locked off mutant was attenuated, and constitutive production of type 1 fimbriae by the phase-locked on...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.2.1387-1393.2006
更新日期:2006-02-01 00:00:00
abstract::The immune mechanism by which hamsters acquire resistance to infection with Treponema pertenue, the causative agent of frambesia, or yaws, has not been elucidated. Serum or cells (spleen or lymph node) obtained from hamsters resistant to frambesial infection were transferred to normal syngenic recipients, who are subs...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.2.430-435.1978
更新日期:1978-08-01 00:00:00
abstract::Pyolysin (PLO), a cholesterol-dependent cytolysin expressed by Arcanobacterium pyogenes, is an important host-protective antigen. However, this molecule is toxic and requires inactivation prior to its use as a vaccine. Three genetically toxoided, nonhemolytic PLO molecules, HIS-PLO.F(497), HIS-PLO.Delta P(499), and HI...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.5.2966-2969.2003
更新日期:2003-05-01 00:00:00
abstract::Neisseria gonorrhoeae is generally considered to be an obligate aerobe; it can, however, grow in the absence of oxygen by anaerobic respiration by using nitrite as a terminal electron acceptor. The outer membrane protein compositions of aerobically and anaerobically grown N. gonorrhoeae strains were compared by one- a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.6.1359-1364.1987
更新日期:1987-06-01 00:00:00
abstract::The outer membrane protein OmpU of Vibrio cholerae O1 strain 86B3 was characterized with reference to colonization of the intestine by the organism. The purified OmpU exhibited a pI of 3.6. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, it migrated to 38, 32, and 110 kDa when the sample was heated at ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.10.4726-4728.1998
更新日期:1998-10-01 00:00:00
abstract::The addition of a sonic extract of Capnocytophaga sputigena to the culture fluid to human fibroblasts resulted in an inhibition of cell proliferation. The inhibition was dose-related (200 micrograms/ml caused a 90% inhibition, and 1,000 micrograms/ml caused a complete cessation of growth). The growth inhibition was no...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.1.271-275.1980
更新日期:1980-01-01 00:00:00
abstract::Malaria elimination efforts would benefit from vaccines that block transmission of Plasmodium falciparum gametocytes from humans to mosquitoes. A clear understanding of gametocyte-specific antibody responses in exposed populations could help determine whether transmission-blocking vaccines (TBV) would be boosted by na...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00644-15
更新日期:2015-11-01 00:00:00
abstract::The antigenically conserved proteins of Treponema pallidum subsp. pallidum and four nonpathogenic cultivatable treponemes were investigated by phase partitioning with the nonionic detergent Triton X-114 and immunoblot analysis. None of the T. pallidum integral membrane proteins identified by phase partitioning (deterg...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.7.1825-1828.1988
更新日期:1988-07-01 00:00:00
abstract::Human immunodeficiency virus (HIV)-infected individuals are predisposed to recurrent oral candidiasis, and, although it has been assumed that this is because of deficient mucosal immune responses, this has not been properly established. The present study aimed to compare the concentrations and secretion rates of immun...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.3.892-896.1994
更新日期:1994-03-01 00:00:00
abstract::Transgenic tobacco plants stably expressing recombinant FaeG, which is the major subunit and adhesin of K88ad fimbriae, were obtained. Analysis of sera from immunized mice indicates that in mice, the immunogenicity induced by plant-derived FaeG protein is comparable to that generated with traditional approaches. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.9.5436-5439.2003
更新日期:2003-09-01 00:00:00
abstract::Changes in cell viability and in factors affecting metabolic integrity were examined after exposure of Escherichia coli LP1092 to human serum. Antibody-dependent classical pathway activity accounted for the rapid killing of strain LP1092 by complement. Removal of serum lysozyme by bentonite absorption or by neutraliza...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.1.122-131.1983
更新日期:1983-01-01 00:00:00
abstract::Streptococcus pneumoniae remains a major causative agent of serious human diseases. The worldwide increase of antibiotic resistant strains revealed the importance of horizontal gene transfer in this pathogen, a scenario that results in the modulation of the species-specific gene pool. We investigated genomic variation...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.2477-2486.2001
更新日期:2001-04-01 00:00:00
abstract::The Pseudomonas aeruginosa quorum-sensing systems, las and rhl, control the production of numerous virulence factors. In this study, we have used the burned-mouse model to examine the contribution of quorum-sensing systems to the pathogenesis of P. aeruginosa infections in burn wounds. Different quorum-sensing mutants...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.5854-5862.1999
更新日期:1999-11-01 00:00:00
abstract::Twenty-six Pseudomonas aeruginosa strains from patients with cystic fibrosis were typed by the Fisher immunotyping scheme. Only 6 strains were agglutinated by a single typing serum, whereas 15 strains were agglutinated with more than one serum and 5 were not agglutinated by any serum. Neither the polyagglutinable nor ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.1.170-177.1983
更新日期:1983-10-01 00:00:00
abstract::A nasal vaccine, consisting of outer membrane vesicles (OMVs) from group B Neisseria meningitidis, was given to 12 volunteers in the form of nose drops or nasal spray four times at weekly intervals, with a fifth dose 5 months later. Each nasal dose consisted of 250 microg of protein, equivalent to 10 times the intramu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1334-1341.1998
更新日期:1998-04-01 00:00:00
abstract::Herein we report an important role for the ferric uptake regulator (Fur) in the resistance of Salmonella enterica serovar Typhimurium to the reactive nitrogen species produced by inducible nitric oxide (NO) synthase in an NRAMP1(r) murine model of acute systemic infection. The expression of fur protected Salmonella gr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01201-13
更新日期:2014-01-01 00:00:00
abstract::The 120-kDa rickettsial outer membrane protein (rOmpB) is encoded by a gene with the capacity to encode a protein of approximately 168 kDa. The carboxy-terminal end of the molecule is apparently cleaved to yield 120- and 32-kDa products. Both polypeptides are surface exposed and remain associated with the outer membra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.1.159-165.1992
更新日期:1992-01-01 00:00:00
abstract::Endotoxemia in rats is associated with the accumulation of neutrophils (polymorphonuclear leukocytes) within the airspaces of the lung. Polymorphonuclear leukocyte influx appears to be regulated by the intrapulmonary accumulation of chemotactic activity. Since alveolar macrophages (AMS) are prevalent cells in the airs...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.3.810-816.1989
更新日期:1989-03-01 00:00:00