当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > R2R3-type MYB transcription factor, CmMYB1, is a central nitrogen assimilation regulator in Cyanidioschyzon merolae.

R2R3-type MYB transcription factor, CmMYB1, is a central nitrogen assimilation regulator in Cyanidioschyzon merolae.

Abstract：

:Plant cells sense environmental nitrogen levels and alter their gene expression accordingly to survive; however, the underlying regulatory mechanisms still remains to be elucidated. Here, we identified and characterized a transcription factor that is responsible for expression of nitrogen assimilation genes in a unicellular red alga Cyanidioschyzon merolae. DNA microarray and Northern blot analyses revealed that transcript of the gene encoding CmMYB1, an R2R3-type MYB transcription factor, increased 1 h after nitrogen depletion. The CmMYB1 protein started to accumulate after 2 h and reached a peak after 4 h after nitrogen depletion, correlating with the expression of key nitrogen assimilation genes, such as CmNRT, CmNAR, CmNIR, CmAMT, and CmGS. Although the transcripts of these nitrogen assimilation genes were detected in nitrate-grown cells, they disappeared upon the addition of preferred nitrogen source such as ammonium or glutamine, suggesting the presence of a nitrogen catabolite repression (NCR) mechanism. The nitrogen depletion-induced gene expression disappeared in a CmMYB1-null mutant, and the mutant showed decreased cell viability after exposure to the nitrogen-depleted conditions compared with the parental strain. Chromatin immunoprecipitation analysis demonstrated that CmMYB1 specifically occupied these nitrogen-responsive promoter regions only under nitrogen-depleted conditions, and electrophoretic mobility shift assays using crude cell extract revealed specific binding of CmMYB1, or a complex containing CmMYB1, to these promoters. Thus, the presented results indicated that CmMYB1 is a central nitrogen regulator in C. merolae.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Imamura S,Kanesaki Y,Ohnuma M,Inouye T,Sekine Y,Fujiwara T,Kuroiwa T,Tanaka K

doi

10.1073/pnas.0902790106

subject

Has Abstract

pub_date

2009-07-28 00:00:00

pages

12548-53

issue

30

eissn

0027-8424

issn

1091-6490

pii

0902790106

journal_volume

106

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • A note on statistical analysis of shape through triangulation of landmarks.

    abstract：:In an earlier paper, the author jointly with S. Suryawanshi proposed statistical analysis of shape through triangulation of landmarks on objects. It was observed that the angles of the triangles are invariant to scaling, location, and rotation of objects. No distinction was made between an object and its reflection. T...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.97.7.2995

    authors： Rao CR

    更新日期：2000-03-28 00:00:00

  • Fibroblast growth factor receptor influences primary cilium length through an interaction with intestinal cell kinase.

    abstract：:Vertebrate primary cilium is a Hedgehog signaling center but the extent of its involvement in other signaling systems is less well understood. This report delineates a mechanism by which fibroblast growth factor (FGF) controls primary cilia. Employing proteomic approaches to characterize proteins associated with the F...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1800338116

    authors： Kunova Bosakova M,Nita A,Gregor T,Varecha M,Gudernova I,Fafilek B,Barta T,Basheer N,Abraham SP,Balek L,Tomanova M,Fialova Kucerova J,Bosak J,Potesil D,Zieba J,Song J,Konik P,Park S,Duran I,Zdrahal Z,Smajs D,Jans

    更新日期：2019-03-05 00:00:00

  • Localization of atherosclerosis susceptibility loci to chromosomes 4 and 6 using the Ldlr knockout mouse model.

    abstract：:Atherosclerosis is a complex disease resulting from the interaction of multiple genes. We have used the Ldlr knockout mouse model in an interspecific genetic cross to map atherosclerosis susceptibility loci. A total of 174 (MOLF/Ei x B6.129S7-Ldlr(tm1Her)) x C57BL/6J-Ldlr(tm1Her) backcross mice, homozygous for the Ldl...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.141239098

    authors： Welch CL,Bretschger S,Latib N,Bezouevski M,Guo Y,Pleskac N,Liang CP,Barlow C,Dansky H,Breslow JL,Tall AR

    更新日期：2001-07-03 00:00:00

  • Differences in spatial versus temporal reaction norms for spring and autumn phenological events.

    abstract：:For species to stay temporally tuned to their environment, they use cues such as the accumulation of degree-days. The relationships between the timing of a phenological event in a population and its environmental cue can be described by a population-level reaction norm. Variation in reaction norms along environmental ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2002713117

    authors： Delgado MDM,Roslin T,Tikhonov G,Meyke E,Lo C,Gurarie E,Abadonova M,Abduraimov O,Adrianova O,Akimova T,Akkiev M,Ananin A,Andreeva E,Andriychuk N,Antipin M,Arzamascev K,Babina S,Babushkin M,Bakin O,Barabancova A,Bas

    更新日期：2020-12-08 00:00:00

  • Transglutaminase 2 is essential for adherence of Porphyromonas gingivalis to host cells.

    abstract：:Porphyromonas gingivalis is the major causative agent of periodontitis, and it may also be involved in the development of systemic diseases (atherosclerosis, rheumatoid arthritis). P. gingivalis is found on and within oral and gingival epithelial cells following binding to surface components of host cells, which serve...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1402740111

    authors： Boisvert H,Lorand L,Duncan MJ

    更新日期：2014-04-08 00:00:00

  • Specific association between an endoribonucleolytic sequence from a satellite RNA and a substrate analogue containing a 2'-5' phosphodiester.

    abstract：:Both polarities of the satellite RNA of tobacco ringspot virus are sources of self-cleaving sequences. RNA of the less abundant, negative polarity, designated sTobRV-(-)RNA, has cleaving activity that was mapped previously to two noncontiguous regions of the polyribonucleotide chain. Endoribonucleolytic oligoribonucle...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.7.2623

    authors： Feldstein PA,Buzayan JM,van Tol H,deBear J,Gough GR,Gilham PT,Bruening G

    更新日期：1990-04-01 00:00:00

  • Multiplexed variation scanning for 1,000 amplicons in hundreds of patients using mismatch repair detection (MRD) on tag arrays.

    abstract：:Identification of the genetic basis of common disease may require comprehensive sequence analysis of coding regions and regulatory elements in patients and controls to find genetic effects caused by rare or heterogeneous mutations. In this study, we demonstrate how mismatch repair detection on tag arrays can be applie...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0506677102

    authors： Faham M,Zheng J,Moorhead M,Fakhrai-Rad H,Namsaraev E,Wong K,Wang Z,Chow SG,Lee L,Suyenaga K,Reichert J,Boudreau A,Eberle J,Bruckner C,Jain M,Karlin-Neumann G,Jones HB,Willis TD,Buxbaum JD,Davis RW

    更新日期：2005-10-11 00:00:00

  • Biphasic regulation by dibutyryl cyclic AMP of tubulin and actin mRNA levels in neuroblastoma cells.

    abstract：:Blot hybridization analysis that used labeled tubulin cDNA probes revealed that N6,O2'-dibutyryladenosine 3',5'-cyclic monophosphate [dibutyryl cyclic AMP (Bt2cAMP)] initially increases and later decreases the level of tubulin mRNA in a neuroblastoma-glioma hybrid cell line as well as in the parent cells. A significan...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.80.14.4243

    authors： Ginzburg I,Rybak S,Kimhi Y,Littauer UZ

    更新日期：1983-07-01 00:00:00

  • Minus-strand origin of filamentous phage versus transcriptional promoters in recognition of RNA polymerase.

    abstract：:Replication of complementary-strand DNA in filamentous phages is initiated by a primer RNA that is synthesized at the minus-strand origin on the viral single-stranded DNA by Escherichia coli RNA polymerase holoenzyme containing the sigma70 subunit. We have demonstrated that the affinity of RNA polymerase in vitro to t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.7.2909

    authors： Higashitani A,Higashitani N,Horiuchi K

    更新日期：1997-04-01 00:00:00

  • Affinity of IDPs to their targets is modulated by ion-specific changes in kinetics and residual structure.

    abstract：:Intrinsically disordered proteins (IDPs) are characterized by a lack of defined structure. Instead, they populate ensembles of rapidly interconverting conformations with marginal structural stabilities. Changes in solution conditions such as temperature and crowding agents consequently affect IDPs more than their fold...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1705105114

    authors： Wicky BIM,Shammas SL,Clarke J

    更新日期：2017-09-12 00:00:00

  • Regulation of acetate metabolism and coordination with the TCA cycle via a processed small RNA.

    abstract：:Bacterial regulatory small RNAs act as crucial regulators in central carbon metabolism by modulating translation initiation and degradation of target mRNAs in metabolic pathways. Here, we demonstrate that a noncoding small RNA, SdhX, is produced by RNase E-dependent processing from the 3'UTR of the sdhCDAB-sucABCD ope...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1815288116

    authors： De Mets F,Van Melderen L,Gottesman S

    更新日期：2019-01-15 00:00:00

  • Invariant representations of visual patterns in a temporal population code.

    abstract：:Mammalian visual systems are characterized by their ability to recognize stimuli invariant to various transformations. Here, we investigate the hypothesis that this ability is achieved by the temporal encoding of visual stimuli. By using a model of a cortical network, we show that this encoding is invariant to several...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0136977100

    authors： Wyss R,Konig P,Verschure PF

    更新日期：2003-01-07 00:00:00

  • Protacs: chimeric molecules that target proteins to the Skp1-Cullin-F box complex for ubiquitination and degradation.

    abstract：:The intracellular levels of many proteins are regulated by ubiquitin-dependent proteolysis. One of the best-characterized enzymes that catalyzes the attachment of ubiquitin to proteins is a ubiquitin ligase complex, Skp1-Cullin-F box complex containing Hrt1 (SCF). We sought to artificially target a protein to the SCF ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.141230798

    authors： Sakamoto KM,Kim KB,Kumagai A,Mercurio F,Crews CM,Deshaies RJ

    更新日期：2001-07-17 00:00:00

  • Sparse production but preferential incorporation of recently produced naive T cells in the human peripheral pool.

    abstract：:In mice, recent thymic emigrants (RTEs) make up a large part of the naïve T cell pool and have been suggested to be a distinct short-lived pool. In humans, however, the life span and number of RTEs are unknown. Although (2)H(2)O labeling in young mice showed high thymic-dependent daily naïve T cell production, long te...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0709713105

    authors： Vrisekoop N,den Braber I,de Boer AB,Ruiter AF,Ackermans MT,van der Crabben SN,Schrijver EH,Spierenburg G,Sauerwein HP,Hazenberg MD,de Boer RJ,Miedema F,Borghans JA,Tesselaar K

    更新日期：2008-04-22 00:00:00

  • Inactivation of cardiotrophin-like cytokine, a second ligand for ciliary neurotrophic factor receptor, leads to cold-induced sweating syndrome in a patient.

    abstract：:Ciliary neurotrophic factor (CNTF) receptor controls a pathway supporting the differentiation and survival of a wide range of neural cell types during development and in adulthood. Cardiotrophin-like cytokine (CLC)-cytokine-like factor 1 (CLF) composite cytokine is a second ligand for the CNTF alpha-component receptor...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0509598103

    authors： Rousseau F,Gauchat JF,McLeod JG,Chevalier S,Guillet C,Guilhot F,Cognet I,Froger J,Hahn AF,Knappskog PM,Gascan H,Boman H

    更新日期：2006-06-27 00:00:00

  • Protective role for the N-terminal domain of α-dystroglycan in Influenza A virus proliferation.

    abstract：:α-Dystroglycan (α-DG) is a highly glycosylated basement membrane receptor that is cleaved by the proprotein convertase furin, which releases its N-terminal domain (α-DGN). Before cleavage, α-DGN interacts with the glycosyltransferase LARGE1 and initiates functional O-glycosylation of the mucin-like domain of α-DG. Not...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1904493116

    authors： de Greef JC,Slütter B,Anderson ME,Hamlyn R,O'Campo Landa R,McNutt EJ,Hara Y,Pewe LL,Venzke D,Matsumura K,Saito F,Harty JT,Campbell KP

    更新日期：2019-06-04 00:00:00

  • Cardiac hypertrophy and sudden death in mice with a genetically clamped renin transgene.

    abstract：:Several mouse models have already proved valuable for investigating hypertrophic responses to cardiac stress. Here, we characterize one caused by a well defined single copy transgene, RenTgMK, that genetically clamps plasma renin and thence angiotensin II at high levels. All of the transgenic males develop concentric ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0307333101

    authors： Caron KM,James LR,Kim HS,Knowles J,Uhlir R,Mao L,Hagaman JR,Cascio W,Rockman H,Smithies O

    更新日期：2004-03-02 00:00:00

  • Initial electron-transfer in the reaction center from Rhodobacter sphaeroides.

    abstract：:The initial electron transfer steps in the photosynthetic reaction center of the purple bacterium Rhodobacter sphaeroides have been investigated by femtosecond time-resolved spectroscopy. The experimental data taken at various wavelengths demonstrate the existence of at least four intermediate states within the first ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.13.5168

    authors： Holzapfel W,Finkele U,Kaiser W,Oesterhelt D,Scheer H,Stilz HU,Zinth W

    更新日期：1990-07-01 00:00:00

  • The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis.

    abstract：:We report here the reconstitution of a pathway that leads to the apoptotic changes in nuclei by using recombinant DNA fragmentation factor (DFF), a heterodimeric protein of 40 and 45 kDa. Coexpression of DFF40 and DFF45 is required to generate recombinant DFF, which becomes activated when DFF45 is cleaved by caspase-3...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.15.8461

    authors： Liu X,Li P,Widlak P,Zou H,Luo X,Garrard WT,Wang X

    更新日期：1998-07-21 00:00:00

  • A gradient of auxin and auxin-dependent transcription precedes tropic growth responses.

    abstract：:Plants, although sessile, can reorient growth axes in response to changing environmental conditions. Phototropism and gravitropism represent adaptive growth responses induced by changes in light direction and growth axis orientation relative to gravitational direction, respectively. The nearly 80-year-old Cholodny-Wen...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0507127103

    authors： Esmon CA,Tinsley AG,Ljung K,Sandberg G,Hearne LB,Liscum E

    更新日期：2006-01-03 00:00:00

  • In vitro expression in eukaryotic cells of a prion protein gene cloned from scrapie-infected mouse brain.

    abstract：:It has been proposed that the causative agent of scrapie represents a class of infectious particle that is devoid of nucleic acid and that an altered form of the endogenous prion protein (PrP) is the agent. However, it has been difficult to exclude the possibility that PrP purified from scrapie tissues might be contam...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.13.4657

    authors： Caughey B,Race RE,Vogel M,Buchmeier MJ,Chesebro B

    更新日期：1988-07-01 00:00:00

  • Prokaryotic algae associated with Australian proterozoic stromatolites.

    abstract：:Five instances of association between distinctive stromatolites and blue-green algal nannofossils are recorded from a 100-m sequence of carbonate rocks about 1.6 x 10(9) years old, along the south side of Paradise Creek, northwestern Queensland, Australia. No eukaryotes were identified in any of these systematically l...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.69.9.2500

    authors： Licari GR,Cloud P

    更新日期：1972-09-01 00:00:00

  • Catalysis and rotation of F1 motor: cleavage of ATP at the catalytic site occurs in 1 ms before 40 degree substep rotation.

    abstract：:F1, a water-soluble portion of FoF1-ATP synthase, is an ATP hydrolysis-driven rotary motor. The central gamma-subunit rotates in the alpha 3 beta 3 cylinder by repeating the following four stages of rotation: ATP-binding dwell, rapid 80 degrees substep rotation, interim dwell, and rapid 40 degrees substep rotation. At...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2434983100

    authors： Shimabukuro K,Yasuda R,Muneyuki E,Hara KY,Kinosita K Jr,Yoshida M

    更新日期：2003-12-09 00:00:00

  • Distribution and activity of endogenous lectin during myogenesis as measured with antilectin antibody.

    abstract：:Antibodies to electrolectin, a lectin endogenous to embryonic skeletal muscle, have been used to study the distribution of electrolectin during myogenesis in L6 cells and rat primary muscle cultures. Antibody binding is highest to mononucleated cells and is low to myotubes in both systems. Binding is much lower to fib...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.77.2.1054

    authors： Podleski TR,Greenberg I

    更新日期：1980-02-01 00:00:00

  • Granulocyte colony-stimulating factor enhances interleukin 3-dependent proliferation of multipotential hemopoietic progenitors.

    abstract：:In cultures of spleen cells from normal mice, recombinant human granulocyte colony-stimulating factor (G-CSF) supported the formation of multipotential blast cell colonies. Serial replating of the blast cell colonies in the presence of G-CSF, however, failed to demonstrate any direct effect of G-CSF on murine multipot...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.10.3445

    authors： Ikebuchi K,Clark SC,Ihle JN,Souza LM,Ogawa M

    更新日期：1988-05-01 00:00:00

  • Natal dispersal driven by environmental conditions interacting across the annual cycle of a migratory songbird.

    abstract：:Natal dispersal, the process through which immature individuals permanently depart their natal area in search of new sites, is integral to the ecology and evolution of animals. Insights about the underlying causes of natal dispersal arise mainly from research on species whose short dispersal distances or restricted di...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0710732105

    authors： Studds CE,Kyser TK,Marra PP

    更新日期：2008-02-26 00:00:00

  • Inferring the shape of global epistasis.

    abstract：:Genotype-phenotype relationships are notoriously complicated. Idiosyncratic interactions between specific combinations of mutations occur and are difficult to predict. Yet it is increasingly clear that many interactions can be understood in terms of global epistasis. That is, mutations may act additively on some under...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1804015115

    authors： Otwinowski J,McCandlish DM,Plotkin JB

    更新日期：2018-08-07 00:00:00

  • Homeostatic imbalance of regulatory and effector T cells due to IL-2 deprivation amplifies murine lupus.

    abstract：:The origins and consequences of a regulatory T cell (Treg) disorder in systemic lupus erythematosus (SLE) are poorly understood. In the (NZBxNZW) F(1) mouse model of lupus, we found that CD4(+)Foxp3(+) Treg failed to maintain a competitive pool size in the peripheral lymphoid organs resulting in a progressive homeosta...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0903158107

    authors： Humrich JY,Morbach H,Undeutsch R,Enghard P,Rosenberger S,Weigert O,Kloke L,Heimann J,Gaber T,Brandenburg S,Scheffold A,Huehn J,Radbruch A,Burmester GR,Riemekasten G

    更新日期：2010-01-05 00:00:00

  • The MCK mouse heart model of Friedreich's ataxia: Alterations in iron-regulated proteins and cardiac hypertrophy are limited by iron chelation.

    abstract：:There is no effective treatment for the cardiomyopathy of the most common autosomal recessive ataxia, Friedreich's ataxia (FA). The identification of potentially toxic mitochondrial (MIT) iron (Fe) deposits in FA suggests that Fe plays a role in its pathogenesis. This study used the muscle creatine kinase conditional ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0804261105

    authors： Whitnall M,Suryo Rahmanto Y,Sutak R,Xu X,Becker EM,Mikhael MR,Ponka P,Richardson DR

    更新日期：2008-07-15 00:00:00

  • A "public" T-helper epitope of the E7 transforming protein of human papillomavirus 16 provides cognate help for several E7 B-cell epitopes from cervical cancer-associated human papillomavirus genotypes.

    abstract：:We have identified a major T-cell epitope, amino acids 48-54 (DRAHYNI, in one-letter code) in the E7 open reading frame protein of human papillomavirus (HPV) type 16. Lymph node cells from mice immunized with synthetic peptides containing DRAHYNI proliferated and produced interleukin when challenged in vitro with pept...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.13.5887

    authors： Tindle RW,Fernando GJ,Sterling JC,Frazer IH

    更新日期：1991-07-01 00:00:00