Abstract:
:Neuroglobin (Ngb) is a tissue globin specifically expressed in neurons. Our laboratory and others have shown that Ngb overexpression protects neurons against hypoxia/ischemia, but the underlying mechanisms remain poorly understood. Recent studies demonstrate that hypoxia/ischemia induces a multitude of spatially and temporally regulated responses in gene expression, and initial evidence suggested that Ngb might function in altering biological processes of gene expression. In this study, we asked how Ngb may help regulate genes responsive to hypoxia. Expression of hypoxic response genes following oxygen-glucose deprivation (OGD) was examined using mRNA arrays in neuroglobin-overexpressing transgenic (Ngb-Tg) and wild type (WT) mouse neurons. From a total of 113 genes on the microarray, mRNA expression of 65 genes was detected. Under rest condition, 14 genes were downregulated in Ngb-Tg neurons compared to WT. In WT neurons, after 4-h OGD followed by 4-h reoxygenation (O4/R4), 20 genes were significantly downregulated, and only Fos mRNA was significantly increased. However, out of the 20 downregulated genes in WT neurons, 12 of them were no longer significantly changed in Ngb-Tg neurons: Add1, Arnt2, Camk2g, Cstb, Dr1, Epas1, Gna11, Hif1a, Il6st, Khsrp, Mars and Rara. Among these 12 genes, 8 (Add1, Camk2g, Cstb, Dr1, Epas1, Gna11, Hif1a, Khsrp) were already reduced in Ngb-Tg neurons compared to WT under rest conditions. Additionally, three genes that initially showed no changes in WT neurons (Ctgf, Egfr and Pea15) were downregulated after OGD in the Ngb-Tg neurons. These findings suggest that Ngb overexpression modulates mRNA expression of multiple hypoxic response genes in the early phase after OGD/reoxygenation. Further studies on these gene networks and interactions may lead to better understanding of Ngb in signaling pathways that contribute to neuroprotection.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Yu Z,Liu J,Guo S,Xing C,Fan X,Ning M,Yuan JC,Lo EH,Wang Xdoi
10.1016/j.neuroscience.2009.04.055subject
Has Abstractpub_date
2009-08-18 00:00:00pages
396-403issue
2eissn
0306-4522issn
1873-7544pii
S0306-4522(09)00671-Xjournal_volume
162pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::Chronic Mountain Sickness (CMS) occurs in high-altitude residents with major neurological symptoms such as migraine headaches, dizziness and cognitive deficits. Recent work demonstrated that highlanders have increased intracellular pH (pHi) in their brain cells, perhaps for the sake of adaptation to hypoxemia and help...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2018.02.008
更新日期:2018-04-01 00:00:00
abstract::Excessive elevation of intracellular Ca2+ levels and, subsequently, hyperactivation of Ca2+/calmodulin-dependent processes might play an important role in the pathologic events following cerebral ischemia. PEP-19 is a neuronally expressed polypeptide that acts as an endogenous negative regulator of calmodulin by inhib...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.03.044
更新日期:2008-06-23 00:00:00
abstract::The difference in visual object recognition by males and females suggests a sex-specific function in the medial prefrontal cortex (mPFC). In the present study, we performed an in vivo microdialysis study in three groups of rats (males, diestrous females, and proestrous females) to examine the potential sex difference ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.12.039
更新日期:2009-03-03 00:00:00
abstract::In IMR32 neuroblastoma cells, the two adenosine receptor agonists N6-R-phenylisopropyladenosine and 5'-N-ethylcarboxamidoadenosine dose-dependently stimulated membrane adenylate cyclase activity with potencies consistent with the presence of adenosine receptors of the A2-subtype. The S enantiomer of N6-R-phenylisoprop...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(89)90173-5
更新日期:1989-01-01 00:00:00
abstract::The nucleotide oligomerization domain (NOD)-like receptor (NLR) pyrin domain-containing protein 1 (NLRP1) inflammasome has been shown to contribute to brain injury after ischemic stroke. Our previous study showed that microRNA-9a-5p (miR-9a-5p) ameliorates ischemic injury by regulating neuronal autophagy in rats subje...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2020.01.008
更新日期:2020-09-15 00:00:00
abstract::Central blockade of mineralocorticoid receptors (MRs) or angiotensin II type 1 receptors (AT1Rs) attenuates aldosterone (aldo)-salt induced hypertension. We examined the role of the subfornical organ (SFO), aldo synthesized locally in the brain, and MR and AT1R specifically in the paraventricular nucleus (PVN) in aldo...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.11.055
更新日期:2016-02-09 00:00:00
abstract::The Ca(2+)-dependent release of the neurotransmitter glutamate from purified nerve terminals (synaptosomes) of the rat hippocampus was studied in a rapid perfusion apparatus. The response of the terminals was investigated with respect to the kinetics and duration of the release of endogenous glutamate upon brief and s...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(91)90417-m
更新日期:1991-01-01 00:00:00
abstract::The anatomical localization and pharmacology of alpha5 subunit-containing GABA type-A receptors in the human hippocampal formation of Alzheimer's disease patients were studied with an alpha5 receptor selective ligand, [3H]L-655,708 and compared to age-matched human controls. Autoradiographic analyses revealed a hetero...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(00)00163-9
更新日期:2000-01-01 00:00:00
abstract::In spite of the existence of pedunculopontine tegmental nucleus (PPTg) projections to cerebellar nuclei, their nature and functional role is unknown. These fibers may play a crucial role in postural control and may be involved in the beneficial effects induced by deep-brain stimulation (DBS) of brainstem structures in...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.12.055
更新日期:2016-03-11 00:00:00
abstract::The effect of pairing two vibrissa stimulations on unit responses of the barrel field of the somatosensory cortex were studied in partially restrained but awake and undrugged rats. Before pairing, one of the stimulations (S2) evoked a stable, short-latency and excitatory response from the recorded unit. Depending on t...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(87)90271-5
更新日期:1987-10-01 00:00:00
abstract::We have recently identified a third subtype of glutamate vesicular transporter (VGLUT) named VGLUT3. In the present study, we provide a detailed account of the regional and cellular distributions of VGLUT3 in the rat brain, using specific nucleotide probes and antisera. The distribution of VGLUT3 protein was compared ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2003.10.039
更新日期:2004-01-01 00:00:00
abstract::The cholinergic agonist nicotine facilitates detection of invalidly cued trials in location-cueing paradigms and reduces the associated neural activity in human inferior parietal cortex. By using functional magnetic resonance imaging we test the hypothesis that the nicotinic modulation of attentional reorienting may r...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2007.10.061
更新日期:2008-03-18 00:00:00
abstract::The phenomenon of synesthesia has received a great deal of interest recently in the scientific literature. Many previous studies stressed the unidirectional nature of this phenomenon. For example, color-grapheme synesthetes automatically perceive achromatic numbers as colored (e.g. 7 is turquoise). Conversely, colors ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2005.08.057
更新日期:2006-01-01 00:00:00
abstract::Intracellular microelectrode recordings were used to examine the effects of the potassium channel blockers: 4-aminopyridine, a selective blocker of fast potassium conductances gKf1 and gKf2,13 and tetraethylammonium, a blocker of gKf1, gKf2 and the slow conductance gKs,13 on the repetitive activity of large myelinated...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(95)00139-a
更新日期:1995-09-01 00:00:00
abstract::The majority of work examining the nucleus accumbens core (NAc) has focused on functions pertaining to behaviors guided by appetitive outcomes. These studies have pointed to the NAc as being critical for motivating behavior toward desirable outcomes. For example, we have recently shown that lesions of the NAc impaired...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.11.028
更新日期:2014-01-31 00:00:00
abstract::The rostral ventromedial medulla contains three physiologically defined classes of pain-modulating neuron that project to the spinal and trigeminal dorsal horns. OFF cells contribute to anti-nociceptive processes, ON cells contribute to pro-nociceptive processes (i.e. hyperalgesia) and neutral cells tonically modulate...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(00)00329-8
更新日期:2000-01-01 00:00:00
abstract::Hippocampal anatomy and network organization are capable to generate drug-resistant temporal lobe epilepsy (TLE) in humans and particularly vulnerable to segmental neuronal cell loss. Surgical hippocampectomy has been proven successful in treatment and available human tissue specimens allow systematic clinico-patholog...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.08.003
更新日期:2015-11-19 00:00:00
abstract::When the neural tracer Fluoro-Gold is used to retrogradely label a population of axotomized neurons, cellular labeling can persist in the axotomized nucleus even when Nissl staining indicates that the injured neurons have degenerated. In order to determine the identity of the labeled cells that remain, this study comb...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(91)90096-7
更新日期:1991-01-01 00:00:00
abstract::Considerable evidence suggests that a dysfunction of the dopamine and serotonin (5-hydroxytryptamine or 5-HT) neurotransmitter systems contributes to a diverse range of pathological conditions including schizophrenia, depression and drug abuse. Recent electrophysiological and behavioral studies suggest that 5-HT modul...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(01)00593-0
更新日期:2002-01-01 00:00:00
abstract::The hypocretin/orexin system regulates, among other things, sleep and energy homeostasis. The system is likely regulated by both homeostatic and circadian mechanisms. Little is known about local differences in the regulation of hypocretin activity. The aim of this study was to establish an optimized peptide quantifica...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.09.050
更新日期:2015-12-03 00:00:00
abstract::In this study, using an in vitro superfusion technique for the first time, we provide direct neurochemical evidence of the transmitter role of dopamine at the level of lateral olivocochlear efferent fibres of the guinea-pig cochlea. Our results revealed that nerve terminals are able to take up and release dopamine upo...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00461-8
更新日期:1999-04-01 00:00:00
abstract::Iron-overload can cause cognitive impairment due to blood-brain barrier (BBB) breakdown and brain mitochondrial dysfunction. Although deferiprone (DFP) has been shown to exert neuroprotection, the head-to-head comparison among iron chelators used clinically on brain iron-overload has not been investigated. Moreover, s...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2016.07.003
更新日期:2016-09-22 00:00:00
abstract::To elucidate the role and the mechanism of action of nerve growth factor in the adult central nervous system, we investigated the localization of nerve growth factor-like immunoreactivity in adult rat brain, both quantitatively and immunohistochemically, using polyclonal anti-nerve growth factor immunoglobulin G. We r...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)90204-6
更新日期:1994-05-01 00:00:00
abstract::Superior olivary complex (SOC) neurons receive excitatory and inhibitory inputs from both ears. We determined the nature of such inputs to the main SOC nuclei with an optical imaging system. To do so, brainstem slices of postnatal (P) rats (P3-13) were treated with the fast voltage-sensitive dye RH795, and ipsilateral...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2004.06.012
更新日期:2004-01-01 00:00:00
abstract::Patients with dysphagia can have higher risks of aspiration after repetitive swallowing activity due to the "fatigue effect". However, it is still unknown how consecutive swallows affect brain activity. Therefore, we sought to investigate differences in swallowing brain networks formed during consecutive swallows usin...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2016.11.047
更新日期:2017-03-06 00:00:00
abstract::Depolarization-induced transmitter release in synaptosomes prepared from the hippocampus of aged rats is decreased compared with release from young animals. Although the underlying cause of this deficit is not known, some evidence suggests that increased membrane rigidity may contribute to these age-related synaptic c...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(99)00219-5
更新日期:1999-01-01 00:00:00
abstract::The release of chemical transmitter from nerve terminals is critically dependent on a transient increase in intracellular Ca2+. The increase in Ca2+ may be due to influx of Ca2+ from the extracellular fluid or release of Ca2+ from intracellular stores such as mitochondria. Whether Ca2+ utilized in transmitter release ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(99)00509-6
更新日期:2000-01-01 00:00:00
abstract::Cultured neurons offer many advantages over a slice preparation for whole-cell patch-clamp studies, such as better control over the environment and space clamp control. However, heterogeneous cultures of neurons present problems in distinguishing the cell type from which recordings are made. The present study uses cor...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(99)00153-0
更新日期:1999-01-01 00:00:00
abstract::D2 receptor null mutant (Drd2(-/-)) mice have altered responses to the rewarding and locomotor effects of psychostimulant drugs, which is evidence of a necessary role for D2 receptors in these behaviors. Furthermore, work with mice that constitutively express only the D2 receptor short form (D2S), as a result of genet...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.06.035
更新日期:2013-09-17 00:00:00
abstract::In three rhesus monkeys (Macaca mulatta), the inferior motor cortex was explored by electrical stimulation for sites yielding vocal fold adduction. The retrograde tracer wheat germ-agglutinin-conjugated horseradish peroxidase was injected into the effective sites. Within the forebrain, retrogradely labeled cells were ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2004.06.071
更新日期:2005-01-01 00:00:00