Campylobacter jejuni glycosylation island important in cell charge, legionaminic acid biosynthesis, and colonization of chickens.

Abstract:

:Previously, we identified five genes (Cj1321 to Cj1326, of which Cj1325 and Cj1326 are a single gene) in the O-linked flagellin glycosylation island that are highly prevalent in Campylobacter jejuni isolates from chickens. We report mutagenesis, functional, and structural data to confirm that this locus, and Cj1324 in particular, has a significant contributory role in the colonization of chickens by C. jejuni. A motile DeltaCj1324 mutant with intact flagella was considerably less hydrophobic and less able to autoagglutinate and form biofilms than the parent strain, 11168H, suggesting that the surface charge of flagella of Cj1324-deficient strains was altered. The physical and functional attributes of the parent were restored upon complementation. Structural analysis of flagellin protein purified from the DeltaCj1324 mutant revealed the absence of two legionaminic acid glycan modifications that were present in the parent strain, 11168H. These glycoform modifications were shown to be prevalent in chicken isolates and confirm that differences in the highly variable flagellin glycosylation locus can relate to the strain source. The discovery of molecular mechanisms influencing the persistence of C. jejuni in poultry aids the rational design of approaches to control this problematic pathogen in the food chain.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Howard SL,Jagannathan A,Soo EC,Hui JP,Aubry AJ,Ahmed I,Karlyshev A,Kelly JF,Jones MA,Stevens MP,Logan SM,Wren BW

doi

10.1128/IAI.01425-08

subject

Has Abstract

pub_date

2009-06-01 00:00:00

pages

2544-56

issue

6

eissn

0019-9567

issn

1098-5522

pii

IAI.01425-08

journal_volume

77

pub_type

杂志文章
  • Hemolytically active (acylated) alpha-hemolysin elicits interleukin-1beta (IL-1beta) but augments the lethality of Escherichia coli by an IL-1- and tumor necrosis factor-independent mechanism.

    abstract::Many pathogenic Escherichia coli produce the toxin alpha-hemolysin (Hly), and lipopolysaccharide (LPS), interleukin-1 (IL-1), and tumor necrosis factor (TNF) have all been recognized as important effector molecules during infections by gram-negative organisms. Despite the characterization of many in vitro effects of h...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:

    authors: Gleason TG,Houlgrave CW,May AK,Crabtree TD,Sawyer RG,Denham W,Norman JG,Pruett TL

    更新日期:1998-09-01 00:00:00

  • Interaction of Bartonella henselae with endothelial cells promotes monocyte/macrophage chemoattractant protein 1 gene expression and protein production and triggers monocyte migration.

    abstract::Bacillary angiomatosis (BA), one of the many clinical manifestations resulting from infection with the facultative intracellular bacterium Bartonella henselae, is characterized by angiogenic lesions. Macrophages have been identified as important effector cells contributing to the angiogenic process during B. henselae ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.9.5735-5742.2005

    authors: McCord AM,Burgess AW,Whaley MJ,Anderson BE

    更新日期:2005-09-01 00:00:00

  • Infection-induced airway fibrosis in two rat strains with differential susceptibility.

    abstract::Chronic infections play a significant role in the morbidity and mortality of patients with chronic airflow limitation. By stimulating airway inflammation, persistent infection has the potential to cause airway fibrosis. However, in patient this condition is most typically found in lungs damaged by other factors, such ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.60.7.2936-2942.1992

    authors: McIntosh JC,Simecka JW,Ross SE,Davis JK,Miller EJ,Cassell GH

    更新日期:1992-07-01 00:00:00

  • Molecular characterization of glycoprotein antigens on surface of Treponema pallidum: comparison with nonpathogenic Treponema phagedenis biotype Reiter.

    abstract::Four glycoproteins of Treponema pallidum were identified by intrinsic [14C]glucosamine labeling. Only two glycoproteins were demonstrated in T. phagedenis biotype Reiter with the same technique. Glycoproteins of both treponemes were characterized as antigens and shown to be localized within the outer membranes of the ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.46.3.867-869.1984

    authors: Moskophidis M,Müller F

    更新日期:1984-12-01 00:00:00

  • In vitro response of human T cells to Pseudomonas aeruginosa.

    abstract::Pseudomonas aeruginosa is a gram-negative bacillus that is a major cause of morbidity and mortality in immunosuppressed patients, burn patients, and patients with cystic fibrosis. Although immunity to these bacteria has been associated with serum antibody, more recent evidence suggests that T-cell-mediated immunity ma...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.40.2.670-674.1983

    authors: Porwoll JM,Gebel HM,Rodey GE,Markham RB

    更新日期:1983-05-01 00:00:00

  • Periodontal pathogens invade gingiva and aortic adventitia and elicit inflammasome activation in αvβ6 integrin-deficient mice.

    abstract::The American Heart Association supports an association between periodontal diseases and atherosclerosis but not a causal association. This study explores the use of the integrin β6(-/-) mouse model to study the causality. We investigated the ability of a polymicrobial consortium of Porphyromonas gingivalis, Treponema ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01077-15

    authors: Velsko IM,Chukkapalli SS,Rivera-Kweh MF,Zheng D,Aukhil I,Lucas AR,Larjava H,Kesavalu L

    更新日期:2015-12-01 00:00:00

  • Experimental infection with Trypanosoma cruzi increases the population of CD8(+), but not CD4(+), immunoglobulin G Fc receptor-positive T lymphocytes.

    abstract::It is well established that activating-type Fc receptors for immunoglobulin G (FcgammaR), such as FcgammaRI and FcgammaRIII, are essential for inducing inflammatory responses. On the other hand, a unique inhibitory FcgammaR, FcgammaRIIB, inhibits intracellular signaling upon engagement of immunoglobulin G-immune compl...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.8.5048-5052.2005

    authors: Henriques-Pons A,Olivieri BP,Oliveira GM,Daëron M,de Araújo-Jorge TC

    更新日期:2005-08-01 00:00:00

  • The sigma B regulon influences internalization of Staphylococcus aureus by osteoblasts.

    abstract::Individual strains of Staphylococcus aureus have different capacities to become internalized by osteoblasts. Here we report that the levels of sigma(B) expressed by S. aureus correlate with the capacity of this bacterium to be internalized by osteoblasts. However, sigma(B) is not essential for internalization and does...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.71.7.4167-4170.2003

    authors: Nair SP,Bischoff M,Senn MM,Berger-Bächi B

    更新日期:2003-07-01 00:00:00

  • GNA33 of Neisseria meningitidis is a lipoprotein required for cell separation, membrane architecture, and virulence.

    abstract::GNA33 is a membrane-bound lipoprotein with murein hydrolase activity that is present in all Neisseria species and well conserved in different meningococcal isolates. The protein shows 33% identity to a lytic transglycolase (MltA) from Escherichia coli and has been shown to be involved in the degradation of both insolu...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.72.4.1914-1919.2004

    authors: Adu-Bobie J,Lupetti P,Brunelli B,Granoff D,Norais N,Ferrari G,Grandi G,Rappuoli R,Pizza M

    更新日期:2004-04-01 00:00:00

  • Molecular cloning of an Onchocerca volvulus extracellular Cu-Zn superoxide dismutase.

    abstract::Onchocerca volvulus, a human parasitic nematode, is the third leading cause of preventable blindness worldwide. This study describes the molecular cloning of a novel superoxide dismutase (SOD) from the parasite. This putative O. volvulus extracellular SOD (OvEcSOD) is 628 nucleotides (nt) long, including a 22-nt 5' sp...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.62.2.713-716.1994

    authors: James ER,McLean DC Jr,Perler F

    更新日期:1994-02-01 00:00:00

  • Meningococcal outer membrane vesicle vaccine given intranasally can induce immunological memory and booster responses without evidence of tolerance.

    abstract::We have studied the ability of outer membrane vesicle (OMV) vaccines from Neisseria meningitidis serogroup B to induce vaccine-specific antibody and spleen cell proliferative responses in mice after being administered intranasally (i.n.) and/or subcutaneously (s.c.). A series of four weekly i.n. doses (25 microg) with...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.69.8.5010-5015.2001

    authors: Bakke H,Lie K,Haugen IL,Korsvold GE,Høiby EA,Naess LM,Holst J,Aaberge IS,Oftung F,Haneberg B

    更新日期:2001-08-01 00:00:00

  • Multistage multiantigen heterologous prime boost vaccine for Plasmodium knowlesi malaria provides partial protection in rhesus macaques.

    abstract::A nonhuman primate model for malaria vaccine development allowing reliable, stringent sporozoite challenge and evaluation of both cellular and antibody responses is needed. We therefore constructed a multicomponent, multistage DNA vaccine for the simian malaria species Plasmodium knowlesi including two preerythrocytic...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.69.9.5565-5572.2001

    authors: Rogers WO,Baird JK,Kumar A,Tine JA,Weiss W,Aguiar JC,Gowda K,Gwadz R,Kumar S,Gold M,Hoffman SL

    更新日期:2001-09-01 00:00:00

  • Campylobacter jejuni-induced cytokine responses in avian cells.

    abstract::Campylobacter jejuni is a major cause of human inflammatory enteritis. During the course of human disease numerous proinflammatory cytokines are produced. Little is known, however, about the cytokine responses produced during the interaction of this bacterium with the avian host. Campylobacter has been considered a co...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.4.2094-2100.2005

    authors: Smith CK,Kaiser P,Rothwell L,Humphrey T,Barrow PA,Jones MA

    更新日期:2005-04-01 00:00:00

  • Antibody to virus components in volunteers experimentally infected with human coronavirus 229E group viruses.

    abstract::Antibody rises to various virus subcomponents were measured by enzyme-linked immunosorbent assay in the paired sera of volunteers experimentally infected with human coronavirus 229E group viruses. Most of the antibody made during infection was directed against the virus surface projections, with only small amounts of ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.31.3.845-849.1981

    authors: Macnaughton MR,Hasony HJ,Madge MH,Reed SE

    更新日期:1981-03-01 00:00:00

  • Ultrastructural observations on cellular and subcellular aspects of experimental Mycoplasma pneumoniae disease.

    abstract::The ultrastructural organization of Mycoplasma pneumoniae membranes and spatial relationships of this pathogen to epithelial cells in tracheal organ cultures were examined ultrastructurally by freeze-fracture techniques. Areas of morphologically distinct cell membrane variability characterized by membrane blebs and al...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:

    authors: Carson JL,Collier AM,Hu SC

    更新日期:1980-09-01 00:00:00

  • Anti-Pneumococcal Capsular Polysaccharide Antibody Response and CD5 B Lymphocyte Subsets.

    abstract::The role of CD19(+) CD5(+) and CD19(+) CD5(-) B cell subpopulations in the antibody response to pneumococcal capsular polysaccharides (caps-PSs) is controversial. In the present study, we evaluated the role of human CD19(+) CD5(+) and CD19(+) CD5(-) cell populations in the serotype-specific antibody response to caps-P...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00068-15

    authors: Moens L,Verbinnen B,Covens K,Wuyts G,Johnson M,Roalfe L,Goldblatt D,Meyts I,Bossuyt X

    更新日期:2015-07-01 00:00:00

  • Primary murine Chlamydia trachomatis pneumonia in B-cell-deficient mice.

    abstract::Mice were rendered deficient in B-cell activity by treatment with anti-mu antibody from birth. These animals were then infected intranasally with murine Chlamydia trachomatis (murine pneumonitis agent [MoPn]). They produced neither local nor systemic antibody to MoPn but had intact delayed-type hypersensitivity to MoP...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.55.10.2387-2390.1987

    authors: Williams DM,Grubbs B,Schachter J

    更新日期:1987-10-01 00:00:00

  • Mycobacterium-inducible Nramp in striped bass (Morone saxatilis).

    abstract::In mammals, the natural resistance-associated macrophage protein 1 gene, Nramp1, plays a major role in resistance to mycobacterial infections. Chesapeake Bay striped bass (Morone saxatilis) is currently experiencing an epizootic of mycobacteriosis that threatens the health of this ecologically and economically importa...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.72.3.1626-1636.2004

    authors: Burge EJ,Gauthier DT,Ottinger CA,Van Veld PA

    更新日期:2004-03-01 00:00:00

  • Immunochemical analysis of streptococcal group A, B, and C carbohydrates, with emphasis on group A.

    abstract::Streptococcal group A, B, and C carbohydrates were analyzed by counterimmunoelectrophoresis, immunoelectrophoresis, and inhibition of immunoprecipitation. Extracts of streptococci group A or C were shown by counterimmunoelectrophoresis to contain both anodic and cathodic migrating components. In immunoelectrophoresis,...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.37.1.209-215.1982

    authors: Fung JC,Wicher K,McCarty M

    更新日期:1982-07-01 00:00:00

  • Requirement for an additional serum factor essential for the antibody-independent activation of the classical complement sequence by Gram-negative bacteria.

    abstract::Killing of Salmonella minnesota and Salmonella typhimurium S and R strains in serum of nonimmune humans and guinea pigs was drastically reduced in the selective absence of C1q, C1r, Ca2+, C4, or C2, the components of the classical complement pathway. Binding of C1 and C1q to the S form and six different core-deficient...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.37.3.935-939.1982

    authors: Clas F,Loos M

    更新日期:1982-09-01 00:00:00

  • Lipopolysaccharide-induced biliary factors enhance invasion of Salmonella enteritidis in a rat model.

    abstract::In this study, the role of the hepatobiliary system in the early pathogenesis of Salmonella enteritidis infection was investigated in a rat model. Intravenous (i.v.) challenge with lipopolysaccharide (LPS) has previously been shown to enhance the translocation of normal gut flora. We first confirmed that LPS can simil...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.68.1.1-5.2000

    authors: Islam AF,Moss ND,Dai Y,Smith MS,Collins AM,Jackson GD

    更新日期:2000-01-01 00:00:00

  • Uropathogenic Escherichia coli flagella aid in efficient urinary tract colonization.

    abstract::In the murine model of urinary tract infections (UTI), cystitis by uropathogenic Escherichia coli (UPEC) occurs through an intimate relationship with the bladder superficial umbrella cell entailing cycles of adherence, invasion, intracellular bacterial community (IBC) formation, and dispersal (fluxing) from the intrac...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.11.7657-7668.2005

    authors: Wright KJ,Seed PC,Hultgren SJ

    更新日期:2005-11-01 00:00:00

  • The AraC family transcriptional regulator Rv1931c plays a role in the virulence of Mycobacterium tuberculosis.

    abstract::A Mycobacterium tuberculosis strain disrupted in the AraC homologue Rv1931c was isolated. The mutant strain exhibited reduced survival both in macrophages and in a mouse infection model, with survival being restored on complementation with the Rv1931c gene. These results suggest that Rv1931c regulates genes important ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.72.9.5483-5486.2004

    authors: Frota CC,Papavinasasundaram KG,Davis EO,Colston MJ

    更新日期:2004-09-01 00:00:00

  • Detection of fibrils associated with Rickettsia rickettsii.

    abstract::The ultrastructural appearance of the "halozone" formed at the interface between the spotted fever agent Rickettsia rickettsii and the cytoplasm of persistently infected cultured vole cells (Microtus pennsylvanicus) was studied by transmission electron microscopy. In sections of epoxy-embedded specimens stained with u...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.41.3.1252-1260.1983

    authors: Todd WJ,Burgdorfer W,Wray GP

    更新日期:1983-09-01 00:00:00

  • Serum immune response to Shigella protein antigens in rhesus monkeys and humans infected with Shigella spp.

    abstract::The serum antibody response to proteins encoded by the virulence-associated plasmid of Shigella flexneri was determined in monkeys challenged with virulent S. flexneri serotype 2a. With water-extractable antigen in an enzyme-linked immunosorbent assay, a significant increase in antibody titer against proteins from a p...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.53.1.57-63.1986

    authors: Oaks EV,Hale TL,Formal SB

    更新日期:1986-07-01 00:00:00

  • Survival of Staphylococcus epidermidis in Fibroblasts and Osteoblasts.

    abstract::Staphylococcus epidermidis is a leading cause of infections associated with indwelling medical devices, including prosthetic joint infection. While biofilm formation is assumed to be the main mechanism underlying the chronic infections S. epidermidis causes, we hypothesized that S. epidermidis also evades immune killi...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00237-18

    authors: Perez K,Patel R

    更新日期:2018-09-21 00:00:00

  • Human immune responses to oral microorganisms: patterns of systemic antibody levels to Bacteroides species.

    abstract::Human systemic antibody levels to oral members of the Bacteroides genus were assessed with an enzyme-linked immunosorbent assay. Antibody levels to B. gingivalis, two homology groups of B. intermedius, B. melaninogenicus, B. denticola, B. loescheii, B. corporis, B. oralis, B. buccae, and B. gracilis were determined in...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.51.2.507-513.1986

    authors: Ebersole JL,Taubman MA,Smith DJ,Frey DE

    更新日期:1986-02-01 00:00:00

  • Escherichia coli O157:H7 colonization in cattle following systemic and mucosal immunization with purified H7 flagellin.

    abstract::Escherichia coli O157:H7 is an important pathogen of humans. Cattle are most frequently identified as the primary source of infection, and therefore, reduction in E. coli O157:H7 prevalence in cattle by vaccination represents an attractive strategy for reducing the incidence of human disease. H7 flagella have been imp...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01452-07

    authors: McNeilly TN,Naylor SW,Mahajan A,Mitchell MC,McAteer S,Deane D,Smith DG,Low JC,Gally DL,Huntley JF

    更新日期:2008-06-01 00:00:00

  • Identification and characterization of an autolysin-encoding gene of Streptococcus mutans.

    abstract::We identified a gene (atlA) encoding autolytic activity from Streptococcus mutans Xc. The AtlA protein predicted to be encoded by atlA is composed of 979 amino acids with a molecular weight of 107,279 and has a conserved beta-1,4-N-acetylmuramidase (lysozyme) domain in the C-terminal portion. Sodium dodecyl sulfate ex...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.6.3512-3520.2005

    authors: Shibata Y,Kawada M,Nakano Y,Toyoshima K,Yamashita Y

    更新日期:2005-06-01 00:00:00

  • Tegumental phosphodiesterase SmNPP-5 is a virulence factor for schistosomes.

    abstract::The intravascular trematode Schistosoma mansoni is a causative agent of schistosomiasis, a disease that constitutes a major health problem globally. In this study we cloned and characterized the schistosome tegumental phosphodiesterase SmNPP-5 and evaluated its role in parasite virulence. SmNPP-5 is a 52.5-kDa protein...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.05431-11

    authors: Bhardwaj R,Krautz-Peterson G,Da'dara A,Tzipori S,Skelly PJ

    更新日期:2011-10-01 00:00:00