Abstract:
:As in women receiving oestrogens the administration of 17beta-oestradiol to ovariectomized female rats caused a rise in fasting plasma triglycerides and a fall in plasma glucose. Progesterone, on the other hand, had no significant effects. In the oestradiol treated rats, the portal vein basal insulin levels were slightly reduced. Oestradiol, however, had a marked suppressive effect on the alpha cells of the pancreas resulting in a greater reduction in basal glucagon and impaired glucagon response to alanine infusions. The relative insulin to glucagon (I/G) molar concentration ratio in portal vein blood was increased. Oestradiol also produced a dose dependent increase in the activity of the liver lipogenic enzymes, acetyl CoA carboxylase and fatty acid synthetase. On the other hand, the activity of the gluconeogenic rate limiting enzyme phosphoenol-pyruvate carboxykinase (PEPCK) was inhibited. The cross-over pattern of gluconeogenic intermediates confirmed inhibition of gluconeogenesis at this step, an effect which is similar to that induced by relative insulin 'excess'. Progesterone produced an increase in the portal vein insulin concentrations. Both the basal and the alanine-stimulated glucagon levels were also increased. The I/G molar ratio in portal vein blood of progesterone treated rats remained unaltered and the hepatic lipogenic and gluconeogenic enzyme activities were similar to control animals. These data suggest that insulin activity is increased relative to glucagon in the liver of oestradiol-treated rats due to the rise in portal vein I/G ratio. The changes in liver lipogenic and gluconeogenic enzymes and the alterations in fasting plasma triglycerides and glucose in response to oestrogens could be secondary to this effect. :The effects of 17beta-estradiol (E2) and progesterone (P) on the portal vein blood levels of insulin and glucagon in female ovariectomized (OVX) rats were studied and the simultaneous status of both the rate-limiting enzymes and metabolic intermediates of hepatic lipogenesis and gluconeogenesis were examined. Administration of E2 to OVX rats caused a rise in plasma triglycerides and a fall in plasma glucose. P was without this effect. E2-treated rats had slightly reduced portal vein basal insulin levels and a marked suppression in basal glucagon response with impaired glucagon response to alanine infusions. E2 caused an increase in the relative insulin to glucagon (I/G) molar concentration in portal vein blood and a dose-dependent increase in the activity of acetyl CoA carboxylase and fatty acid synthetase. The activity of the gluconeogenic rate limiting enzyme phosphoenal-pyruvate carboxykinase was inhibited. The inhibition of gluconeogenesis at this point is similar to what occurs with insulin excess. P produced insulin increases in the portal vein and increases in both basal and alanine-stimulated glucagon levels. The I/G ratio remained unchanged, and hepatic lipogenic and gluconeogenic activity were similar to controls. These results suggest that in the liver of E2-treated rats, insulin is increased relative to glucagon due to the rise in portal vein I/G. Other changes could be secondary to this effect.
journal_name
Eur J Clin Investjournal_title
European journal of clinical investigationauthors
Mandour T,Kissebah AH,Wynn Vdoi
10.1111/j.1365-2362.1977.tb01595.xsubject
Has Abstractpub_date
1977-06-01 00:00:00pages
181-7issue
3eissn
0014-2972issn
1365-2362journal_volume
7pub_type
杂志文章abstract::The effect of renal function on the cyclic AMP (cAMP) response to exogenous parathyroid hormone (PTH) was examined in patients with chronic renal failure (n = 22) and primary hyperparathyroidism (n = 19). In the patients with chronic renal failure there was marked resistance to the effect of exogenous PTH. In primary ...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1985.tb00141.x
更新日期:1985-02-01 00:00:00
abstract::During chronic high-altitude (HA) exposure, basal and exercise-induced noradrenaline (NA) increases do not parallel blood pressure (BP) changes observed; unlike beta-adrenergic receptors, to our knowledge no data are available on alpha-receptors. We studied platelet alpha 2- and leucocyte beta-receptors and basal cate...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1046/j.1365-2362.1997.1150663.x
更新日期:1997-04-01 00:00:00
abstract::To assess the renal tubular damage in liver cirrhosis the fractional clearances of beta-2-microglobulin (B2m-fr.cl) and malate-dehydrogenase (MDH-fr.cl) were measured respectively in sixty-four and in forty-six out of seventy-nine patients with liver cirrhosis of different aetiology; furthermore the fractional excreti...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1981.tb01847.x
更新日期:1981-06-01 00:00:00
abstract::The existence of transporters for bile acids (BA) in liver and intestine has been well documented, but information is still needed as to their respective transport capacity. In the present investigation, we compared the hepatic and intestinal transport rates for BA, using perfused livers and intestines. The livers and...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1994.tb01062.x
更新日期:1994-10-01 00:00:00
abstract::Resealed erythrocytes are potential slow release carriers for drugs and enzymes. We have investigated carrier erythrocyte survival in human volunteers using gentamicin (G) as encapsulated cell marker; G was readily incorporated into red cells by hypo-osmotic dialysis (87% efficiency of incorporation) and did not exit ...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1986.tb01305.x
更新日期:1986-02-01 00:00:00
abstract::Postural changes in plasma renin activity were studied in three groups of age and duration-matched male diabetics (potent, impotent and with postural hypotension) and in non-diabetic control subjects. Those diabetic subjects with postural hypotension due to automatic neuropathy had no increase in plasma renin activity...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1976.tb00532.x
更新日期:1976-09-10 00:00:00
abstract:BACKGROUND:We investigated whether plasma ferritin levels through the pro-inflammatory effects of free iron are associated with adipose tissue dysfunction in a relevant population of patients with manifest vascular disease who would potentially benefit the most from further aetiological insights. MATERIALS AND METHODS...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/eci.12165
更新日期:2013-12-01 00:00:00
abstract:BACKGROUND:Complement fragment C5a and neutrophils have been implicated in the pathogenesis of renal disease and C5a has also been shown to delay apoptosis of human neutrophils via a transcription-independent pathway. However, transcription-dependent pathways have not been well described. The present study examined whe...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.2006.01637.x
更新日期:2006-05-01 00:00:00
abstract:BACKGROUND:Bleeding time has been reported to increase during gaseous nitric oxide (NO) inhalation in healthy volunteers and patients, and it has been speculated that inhaled NO inhibits platelet function. However, results have not been unanimous, and we have been unable to document any effects of inhaled NO on circula...
journal_title:European journal of clinical investigation
pub_type: 临床试验,杂志文章
doi:10.1046/j.1365-2362.1999.00560.x
更新日期:1999-11-01 00:00:00
abstract::Antibodies against prostaglandins (PG)F2alpha, E1 and E2 were obtained inrabbits immunized with respectively PG F2alpha, PG E1 and PG E2 conjugated to bovine serum albumin by carbodiimide. A radioimmunoassay capable of measuring 7 pg of PG Falpha, 2 pg of PG E2 and 14 pg of PG E1 in human peripheral plasma is describe...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1975.tb00459.x
更新日期:1975-07-29 00:00:00
abstract::In systemic lupus erythematosus (SLE) patients, most of the clinical manifestation share a vascular component triggered by endothelial dysfunction. Endothelial cells (ECs) activation occurs both on the arterial and venous side, and the high vascular density of kidneys accounts for the detrimental outcomes of SLE throu...
journal_title:European journal of clinical investigation
pub_type: 杂志文章,评审
doi:10.1111/eci.13441
更新日期:2021-02-01 00:00:00
abstract:BACKGROUND:Hyperlipidemia is a common feature of the nephrotic syndrome (NS). From retrospective studies, it has been suggested that aggressive lipid-lowering with low-density lipoprotein apheresis (LDL-A) may not only improve dyslipidemia but also decrease urinary albumin excretion and increase serum levels of albumin...
journal_title:European journal of clinical investigation
pub_type: 临床试验,杂志文章
doi:10.1046/j.1365-2362.2000.00716.x
更新日期:2000-10-01 00:00:00
abstract::The effect of infusions of recombinant insulin-like growth factor-I (IGF-I) (34, 103 or 688 pmol min-1 kg-1), insulin (3.4, 10.3 or 68.8 pmol min-1 kg-1) or combined infusions (34 pmol IGF-I + 3.4 pmol min-1 kg-1 insulin or 103 pmol IGF-I + 3.4 pmol min-1 kg-1 insulin) on protein metabolism, using an infusion of [1-14...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1994.tb01094.x
更新日期:1994-05-01 00:00:00
abstract:METHODS:Ten samples of decidua basalis from pre-eclamptic women and 10 from healthy primigravid women subjected to caesarean section (control tissues) were investigated immunohistochemically for changes in angiogenesis and expression of tenascin, an extracellular matrix protein thought to modulate angiogenesis. In addi...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1046/j.1365-2362.1998.00294.x
更新日期:1998-05-01 00:00:00
abstract::The collagenolytic activities were assayed in fibroblast cultures established from ten keratoconus and six control corneal explants. The assays included those for both type I and type IV collagenase. The amount of free, directly assayable type I collagenase was highly increased in the media of keratoconus primary cell...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1986.tb01311.x
更新日期:1986-02-01 00:00:00
abstract:BACKGROUND:It is well known that space travel cause post-flight orthostatic hypotension and it was assumed that autonomic cardiovascular control deteriorates in space. Lower body negative pressure (LBNP) was used to assess autonomic function of the cardiovascular system. METHODS:LBNP tests were performed on six crew-m...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1046/j.1365-2362.2000.00750.x
更新日期:2000-12-01 00:00:00
abstract::In contrast to bile salts, which undergo a highly efficient enterohepatic circulation with multiple regulatory and physiologic functions, glucuronic acid conjugates of bilirubin are biliary excretory molecules that in health do not have a continuing biologic life. Intestinal absorptive cells are devoid of recapture tr...
journal_title:European journal of clinical investigation
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2362.2003.01214.x
更新日期:2003-09-01 00:00:00
abstract::With advancing age both sexes have an increased incidence of osteoporotic fractures, although fractures are more common in women than in men. Whereas in women several potential risk factors have been identified, less is known about osteoporosis in men. A total of 27 Austrian men (mean age: 65 +/- 2 years) with atrauma...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1992.tb01503.x
更新日期:1992-08-01 00:00:00
abstract:BACKGROUND:Changes in bile acid (BA) pool, such as the reappearance of typically foetal-type molecular species with a 'flat' structure at the steroid ring, occur during hepatocarcinogenesis, both in humans and rodents. Moreover flat-BAs also appear during rat liver regeneration. These changes can be detected in urine. ...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.2008.02059.x
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:CD33 (Siglec-3) is becoming increasingly important as a target of antibody-mediated therapy in acute myeloid leukaemia (AML). In normal myelopoiesis, expression of CD33 is restricted to advanced stages of differentiation, whereas primitive stem cells do not express CD33. In the present study, we asked whethe...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.2007.01746.x
更新日期:2007-01-01 00:00:00
abstract::The aim of this study was to assess the effect of intravenous dextran on bacterial translocation and intestinal vascular endothelial and epithelial barrier function after experimental partial hepatectomy. We determined systemic arterial pressure, enteric bacterial growth (proximal and distal small intestine and colon)...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1046/j.1365-2362.1997.2030750.x
更新日期:1997-11-01 00:00:00
abstract:BACKGROUND:Alcohol may have a cardioprotective effect. One possible mechanism is by modifying insulin resistance/secretion. The aims of this study were: (i) to examine the effect of short-term alcohol consumption on the metabolic control of glucose tolerance; (ii) to study the influence of short-term alcohol consumptio...
journal_title:European journal of clinical investigation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1365-2362.2002.00970.x
更新日期:2002-03-01 00:00:00
abstract:OBJECTIVE:N(ε)-(carboxymethyl)lysine (CML) is one of the major advanced glycation end products in both diabetics and nondiabetics. CML depositions in the microvasculature have recently been linked to the aetiology of acute myocardial infarction and cognitive impairment in Alzheimer's disease, possibly related to local ...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/eci.12599
更新日期:2016-04-01 00:00:00
abstract::Although in therapeutic use for more than a century, the mode of cellular action of organic nitrates remains incompletely understood. Despite ample experimental evidence from animal studies to show that nitrates are metabolized to NO in the vascular smooth muscle, direct demonstration of such an activity in human vasc...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1995.tb01952.x
更新日期:1995-10-01 00:00:00
abstract::To diagnose hypolactasia, determination of lactase enzyme activity in small intestinal biopsy material is considered to be the golden standard. Because of its strongly invasive character and the sampling problems, alternative methods have been looked for. We analysed the 13C-glucose response in serum after consumption...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1046/j.1365-2362.2001.00791.x
更新日期:2001-03-01 00:00:00
abstract::The aim of this research was to study exogenous prostacyclin effect on urinary kallikrein excretion (UKK) in man, to define whether prostacyclin-induced renin release and/or endogenously released cyclooxygenase products were responsible for prostacyclin-induced enhancement of UKK, to determine furosemide effect on UKK...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1986.tb01334.x
更新日期:1986-06-01 00:00:00
abstract:BACKGROUND:Bone marrow (BM) histology/immunohistochemistry, KIT D816V mutation analysis and serum tryptase measurements are mandatory tools for diagnosis of systemic mastocytosis (SM). MATERIALS AND METHODS:Within the 'German Registry of Disorders on Eosinophils and Mast Cells', we identified 65 patients with SM who h...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/eci.12607
更新日期:2016-05-01 00:00:00
abstract::Metabolic alterations related to resting energy expenditure (REE) and fat metabolism have been noted during sepsis, and often depend on the causative agent and the stage and severity of the illness. We studied the effect of IL-1, the protein mediator of the 'acute phase' response during infection, on REE, respiratory ...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1987.tb01149.x
更新日期:1987-12-01 00:00:00
abstract:BACKGROUND:Adiponectin is an insulin-sensitizing, antiatherogenic and anti-inflammatory adipocytokine that circulates in three isoforms: a trimer [low-molecular weight (LMW)], a hexamer (trimer-dimer) of medium molecular weight (MMW) and a multimeric high molecular weight (HMW) isoform. Evidence is accumulating that HM...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.2008.02028.x
更新日期:2008-11-01 00:00:00
abstract::We have minimized methodological errors in the isotope dilution technique by using stable isotope, [6,6-2H2]glucose, thus avoiding the problem of contamination of tritiated glucose tracers and, by maintaining a constant plasma tracer enrichment have reduced error due to mixing transients. Using these modifications we ...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1992.tb01458.x
更新日期:1992-04-01 00:00:00