Tumoricidal activity and cytokine secretion by tumor-infiltrating macrophages.


:Murine macrophages from different anatomical sites were compared for their ability to become tumoricidal and to secrete interleukin-1 (IL-1) and tumor necrosis factor (TNF) following stimulation in vitro by several biological response modifiers (BRM). Peritoneal macrophages (PM), alveolar macrophages (AM), and tumor-infiltrating-macrophages (TIM), isolated from B16F10 melanoma colonies in the lung, were incubated overnight with BRM [recombinant murine interferon gamma (rMulFN-gamma), lipopolysaccharide (LPS), muramyl dipeptide (MDP)], either alone or in combination. PM exhibited an increased cytotoxic response following incubation with LPS or rMuIFN-gamma but not with MDP. Both AM and TIM were induced to become tumoricidal following incubation with rMuIFN-gamma plus LPS or rMuIFN-gamma plus MDP but not after stimulation with any BRM alone; the level of cytotoxicity obtained with TIM incubated with rMuIFN-gamma plus LPS was slightly lower than that observed with PM or AM, while with rMuIFN-gamma plus MDP both AM and TIM had lower cytotoxicity than PM. Secretion of IL-I and TNF was observed in PM stimulated with LPS or MDP but not with rMuIFN-gamma. Likewise, secretion of IL-I by AM or TIM was also induced with LPS, although less than that obtained with PM. AM stimulated with LPS secreted larger amounts of TNF than PM while TIM secreted very low amounts of TNF. However, this result may be a consequence of the enzymatic isolation procedure used to obtain TIM since TNF secretion was also impaired in LPS-stimulated normal lung macrophages isolated by a similar enzymatic procedure, or enzyme-treated PM. Our results suggest that TIM obtained from lung metastases share certain functional characteristics with normal AM and respond to BRM in like manner with respect to induction of tumoricidal activity and cytokine secretion.


Int J Cancer


Brunda MJ,Sulich V,Wright RB,Palleroni AV




Has Abstract


1991-07-09 00:00:00












  • Prospective study of oral microbiome and colorectal cancer risk in low-income and African American populations.

    abstract::Oral microbiome may play an important role in cancer pathogenesis. However, no study has prospectively investigated the association of the oral microbiome with subsequent risk of developing colorectal cancer (CRC). We conducted a nested case-control study including 231 incident CRC cases and 462 controls within the So...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Yang Y,Cai Q,Shu XO,Steinwandel MD,Blot WJ,Zheng W,Long J

    更新日期:2019-05-15 00:00:00

  • BYL719, a new α-specific PI3K inhibitor: single administration and in combination with conventional chemotherapy for the treatment of osteosarcoma.

    abstract::It has been established that disturbances in intracellular signaling pathways play a considerable part in the oncologic process. Phosphatidylinositol-3-kinase (PI3K) has become of key interest in cancer therapy because of its high mutation frequency and/or gain in function of its catalytic subunits in cancer cells. We...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Gobin B,Huin MB,Lamoureux F,Ory B,Charrier C,Lanel R,Battaglia S,Redini F,Lezot F,Blanchard F,Heymann D

    更新日期:2015-02-15 00:00:00

  • New pharmacological effect of fulvestrant to prevent oxaliplatin-induced neurodegeneration and mechanical allodynia in rats.

    abstract::Oxaliplatin, which is widely used as chemotherapy for certain solid cancers, frequently causes peripheral neuropathy. Commonly described neuropathic symptoms include aberrant sensations such as mechanical allodynia (hypersensitivity to normally innocuous stimuli). Although oxaliplatin neuropathy is a dose-limiting tox...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Yamamoto S,Yamashita T,Ito M,Caaveiro JMM,Egashira N,Tozaki-Saitoh H,Tsuda M

    更新日期:2019-10-15 00:00:00

  • Novel ovarian cancer maintenance therapy targeted at mortalin and mutant p53.

    abstract::Current ovarian cancer maintenance therapy is limited by toxicity and no proven impact on overall survival. To study a maintenance strategy targeted at missense mutant p53, we hypothesized that the release of mutant p53 from mortalin inhibition by the SHetA2 drug combined with reactivation of mutant p53 with the PRIMA...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ramraj SK,Elayapillai SP,Pelikan RC,Zhao YD,Isingizwe ZR,Kennedy AL,Lightfoot SA,Benbrook DM

    更新日期:2020-08-15 00:00:00

  • Cysteine proteinases produced by cultured rabbit V2 carcinoma cells and rabbit skin fibroblasts.

    abstract::Rabbit V2 carcinoma cells and normal rabbit skin fibroblasts produced cysteine proteinases with properties similar to those of purified rabbit liver cathepsin B. Both cell types secreted into the culture medium enzymes with an apparent Mr of 43,000, which reacted with synthetic substrates commonly used for cathepsin B...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Baici A,Knöpfel M

    更新日期:1986-11-15 00:00:00

  • Intra-tumoral application of a heregulin-exotoxin-a fusion protein causes rapid tumor regression without adverse systemic or local effects.

    abstract::Tumor toxins are recombinant, bifunctional proteins which comprise a tumor-cell-specific recognition domain and an enzymatic toxin domain. We have evaluated the in vivo effects of a tumor toxin that specifically recognizes the erbB-3 and erbB-4 receptors (HRG beta 1-ETA). High doses of HRG beta 1-ETA administered syst...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Groner B,Wick B,Jeschke M,Fiebig HH,Dengler W,Runau T,Mihatsch M,Kahl R,Schmidt M,Wels W,Stöcklin E

    更新日期:1997-03-17 00:00:00

  • Relationship between ornithine decarboxylase levels in anaplastic gliomas and progression-free survival in patients treated with DFMO-PCV chemotherapy.

    abstract::The purpose of this study was to assess the relationship between progression-free survival (PFS) in patients treated with DFMO + PCV (procarbazine, CCNU, vincristine) chemotherapy for malignant gliomas with tumor cell ornithine decarboxylase (ODC) activity. Formalin-fixed slides were obtained for study patients with a...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Levin VA,Jochec JL,Shantz LM,Aldape KD

    更新日期:2007-11-15 00:00:00

  • Loss of heterozygosity and tumor suppressor activity of Bin1 in prostate carcinoma.

    abstract::The genetic events underlying the development of prostate cancer are poorly defined. c-Myc is often activated in tumors that have progressed to metastatic status, so events that promote this process may be important. Bin1 is a nucleocytoplasmic adaptor protein with features of a tumor suppressor that was identified th...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ge K,Minhas F,Duhadaway J,Mao NC,Wilson D,Buccafusca R,Sakamuro D,Nelson P,Malkowicz SB,Tomaszewski J,Prendergast GC

    更新日期:2000-04-15 00:00:00

  • A prospective cohort study of red wine consumption and risk of prostate cancer.

    abstract::In light of recent, strong inverse findings between lifetime red wine consumption and prostate cancer among younger men, we revisited our previous cohort analysis to more thoroughly investigate red wine consumption and prostate cancer in the Health Professionals Follow-up Study (HPFS). In 1986, HPFS participants repor...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Sutcliffe S,Giovannucci E,Leitzmann MF,Rimm EB,Stampfer MJ,Willett WC,Platz EA

    更新日期:2007-04-01 00:00:00

  • IGFBP7 downregulation is associated with tumor progression and clinical outcome in hepatocellular carcinoma.

    abstract::Insulin-like growth factor-binding protein 7 (IGFBP7) functions in several cellular processes including proliferation, senescence and apoptosis. This study analyzed IGFBP7 function in hepatocellular carcinoma (HCC) cells by gene manipulation and investigated the prognostic significance of IGFBP7 expression in clinical...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Tomimaru Y,Eguchi H,Wada H,Kobayashi S,Marubashi S,Tanemura M,Umeshita K,Kim T,Wakasa K,Doki Y,Mori M,Nagano H

    更新日期:2012-01-15 00:00:00

  • Deficiency in surface expression of E-selectin ligand promotes lung colonization in a mouse model of breast cancer.

    abstract::Expression of sialyl Lewis(x) (sLe(x)) and sLe(a) on tumor cells is thought to facilitate metastasis by promoting cell adhesion to selectins on vascular endothelial cells. Experiments supporting this concept usually bypass the early steps of the metastatic process by employing tumor cells that are injected directly in...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Monzavi-Karbassi B,Whitehead TL,Jousheghany F,Artaud C,Hennings L,Shaaf S,Slaughter A,Korourian S,Kelly T,Blaszczyk-Thurin M,Kieber-Emmons T

    更新日期:2005-11-10 00:00:00

  • Differential utilization of the SV40 enhancer and early promoter in various human lung carcinoma cell lines.

    abstract::Cell lines derived from the major morphological types of human lung cancer were tested for their ability to utilize the SV40 enhancer/early promoter. These cell lines were transfected with a recombinant plasmid containing a reporter gene, coding for chloramphenicol acetyltransferase (CAT), under the control of the SV4...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Wong YC,Bernal SD

    更新日期:1990-05-15 00:00:00

  • The mechanism of exogenous B7.1-enhanced IL-12-mediated complete regression of tumors by a single electroporation delivery.

    abstract::Electroporation-based mono-gene therapy has received great interest in recent years but coadministration of different therapeutic genes for treatment of tumors has not been well explored. We hypothesize that electroporation is capable of delivering multiple genes that induce an additive or synergistic antitumor effect...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Liu J,Xia X,Torrero M,Barrett R,Shillitoe EJ,Li S

    更新日期:2006-11-01 00:00:00

  • The first study on therapeutic efficacies of a vascular disrupting agent CA4P among primary hepatocellular carcinomas with a full spectrum of differentiation and vascularity: Correlation of MRI-microangiography-histopathology in rats.

    abstract::To better inform the next clinical trials of vascular disrupting agent combretastatin-A4-phosphate (CA4P) in patients with hepatic malignancies, this preclinical study aimed at evaluating CA4P therapeutic efficacy in rats with primary hepatocellular carcinomas (HCCs) of a full spectrum of differentiation and vasculari...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Liu Y,De Keyzer F,Wang Y,Wang F,Feng Y,Chen F,Yu J,Liu J,Song S,Swinnen J,Bormans G,Oyen R,Huang G,Ni Y

    更新日期:2018-10-01 00:00:00

  • The 4th nation-wide study of adult T-cell leukemia/lymphoma (ATL) in Japan: estimates of risk of ATL and its geographical and clinical features. The T- and B-cell Malignancy Study Group.

    abstract::To estimate the annual incidence of adult T-cell leukemia/lymphoma (ATL) by district in Japan, a large-scale nationwide survey of ATL and of non-Hodgkin's lymphoma was performed in 1988. Questionnaires for the registration of ATL and of T-cell and non-T-cell lymphoma were distributed to the physicians in charge of thi...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Tajima K

    更新日期:1990-02-15 00:00:00

  • Activation of protein kinase C modulates cell-cell and cell-substratum adhesion of a human colorectal carcinoma cell line and restores 'normal' epithelial morphology.

    abstract::Abnormal cell adhesion is an important contributing factor in invasion and metastasis. Here, we show that morphologically 'normal' cell-cell and cell-substratum adhesion can be restored to a poorly differentiated carcinoma cell line by activation of protein kinase C (PKC). This cell line, VACO 10MS, grows as multicell...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Cowell HE,Garrod DR

    更新日期:1999-01-29 00:00:00

  • alpha-fetoprotein-containing cells in the early stages of liver carcinogenesis induced by 3'-methyl-4-dimethyl-aminoazobenzene and 2-acetylaminofluorene.

    abstract::The morphology of rat liver cells containing alpha-feto-protein (AFP) during early stages of carcinogenesis induced by 3'methyl-4-dimethylaminoazobenzene and 2-acetylaminofluorene was investigated. Indirect immunofluorescence was used to detect AFP. AFP was not found in the cells of hyperplastic nodules but was presen...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Tchipysheva TA,Guelstein VI,Bannikov GA

    更新日期:1977-09-15 00:00:00

  • Human papillomavirus prevalence and type distribution in invasive cervical cancer in sub-Saharan Africa.

    abstract::In sub-Saharan Africa, invasive cervical cancer (ICC) incidence and mortality are among the highest in the world. This cross-sectional epidemiological study assessed human papillomavirus (HPV) prevalence and type distribution in women with ICC in Ghana, Nigeria, and South Africa. Cervical biopsy specimens were obtaine...

    journal_title:International journal of cancer

    pub_type: 杂志文章,多中心研究


    authors: Denny L,Adewole I,Anorlu R,Dreyer G,Moodley M,Smith T,Snyman L,Wiredu E,Molijn A,Quint W,Ramakrishnan G,Schmidt J

    更新日期:2014-03-15 00:00:00

  • Folate intake and colorectal cancer risk: a meta-analytical approach.

    abstract::Adequate consumption of folate may reduce the risk of colorectal cancer. We performed a meta-analysis of 7 cohort and 9 case-control studies that examined the association between folate consumption and colorectal cancer risk. In cohort studies, the association between folate consumption and colorectal cancer risk was ...

    journal_title:International journal of cancer

    pub_type: 杂志文章,meta分析


    authors: Sanjoaquin MA,Allen N,Couto E,Roddam AW,Key TJ

    更新日期:2005-02-20 00:00:00

  • Key signaling pathways in the muscle-invasive bladder carcinoma: Clinical markers for disease modeling and optimized treatment.

    abstract::In this review, we evaluate key molecular pathways and markers of muscle-invasive bladder cancer (MIBC). Overexpression and activation of EGFR, p63, and EMT genes are suggestive of basal MIBC subtype generally responsive to chemotherapy. Alterations in PPARγ, ERBB2/3, and FGFR3 gene products and their signaling along ...

    journal_title:International journal of cancer

    pub_type: 杂志文章,评审


    authors: Kiselyov A,Bunimovich-Mendrazitsky S,Startsev V

    更新日期:2016-06-01 00:00:00

  • Novel senescence associated gene, YPEL3, is repressed by estrogen in ER+ mammary tumor cells and required for tamoxifen-induced cellular senescence.

    abstract::Estrogen signaling plays an important role in breast carcinogenesis. An increased understanding of estrogen gene targets and their effects will allow for more directed and effective therapies for individuals with breast cancer, particularly those with estrogen receptor positive tumors resistant to tamoxifen therapy. H...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Tuttle R,Miller KR,Maiorano JN,Termuhlen PM,Gao Y,Berberich SJ

    更新日期:2012-05-15 00:00:00

  • Upregulation of matrix metalloproteinases (MMPs) in breast cancer xenografts: a major induction of stromal MMP-13.

    abstract::In human breast cancer (HBC), as with many carcinoma systems, most matrix metalloproteinases (MMPs) are largely expressed by the stromal cells, whereas the tumour cells are relatively silent in MMP expression. To determine the tissue source of the most relevant MMPs, we xenografted HBC cell lines and HBC tissues into ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Lafleur MA,Drew AF,de Sousa EL,Blick T,Bills M,Walker EC,Williams ED,Waltham M,Thompson EW

    更新日期:2005-04-20 00:00:00

  • Transactivation of MCP-1/CCL2 by beta-catenin/TCF-4 in human breast cancer cells.

    abstract::The loss of E-cadherin expression and the translocation of beta-catenin to the nucleus are frequently associated with the metastatic conversion of epithelial cells. In the nucleus, beta-catenin binds to the TCF/LEF-1 (T-cell factor/ lymphoid enhancer factor) transcription factor family resulting in the activation of s...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Mestdagt M,Polette M,Buttice G,Noël A,Ueda A,Foidart JM,Gilles C

    更新日期:2006-01-01 00:00:00

  • Cell-mediated immune reactivity of Sinclair melanoma-bearing swine to 3 M KCl extracts of swine and human melanoma.

    abstract::Leukocyte migration inhibition (LMI) assays were performed using 3 M KCI extracts to detect cell-mediated immune reactions against tumor-associated antigens (TAA) of swine melanoma. Positive LMI reactivity was 70% or greater in melanoma-bearing swine compared to 10% reactivity or less in normal swine tested with extra...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hook RR Jr,Hamby CV,Millikan LE,Berkelhammer J,Stills HF Jr,Oxenhandler RW

    更新日期:1983-05-15 00:00:00

  • Evaluation of genetic melanoma vaccines in cdk4-mutant mice provides evidence for immunological tolerance against authochthonous melanomas in the skin.

    abstract::We evaluated the efficacy of a candidate melanoma vaccine approach in mice genetically prone to develop melanoma due to the introduction of an oncogenic mutation (R24C) in the germline sequence of the cyclin-dependent kinase 4 (cdk4), a protein critically involved in cell cycle regulation. Melanomas were induced in cd...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Steitz J,Büchs S,Tormo D,Ferrer A,Wenzel J,Huber C,Wölfel T,Barbacid M,Malumbres M,Tüting T

    更新日期:2006-01-15 00:00:00

  • Scatter factor modulates the metastatic phenotype of the EMT6 mouse mammary tumor.

    abstract::EMT6 is a transplantable mouse mammary tumor cell line that has been utilized widely as a model system to study the effects of various treatments on local tumor growth and pulmonary metastasis. In this study, we examined the cellular mechanisms by which scatter factor (SF), a fibroblast-derived cytokine that stimulate...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Rosen EM,Knesel J,Goldberg ID,Jin L,Bhargava M,Joseph A,Zitnik R,Wines J,Kelley M,Rockwell S

    更新日期:1994-06-01 00:00:00

  • Birth order and risk of childhood cancer: a pooled analysis from five US States.

    abstract::The causes of childhood cancers are largely unknown. Birth order has been used as a proxy for prenatal and postnatal exposures, such as frequency of infections and in utero hormone exposures. We investigated the association between birth order and childhood cancers in a pooled case-control dataset. The subjects were d...

    journal_title:International journal of cancer

    pub_type: 杂志文章,多中心研究


    authors: Von Behren J,Spector LG,Mueller BA,Carozza SE,Chow EJ,Fox EE,Horel S,Johnson KJ,McLaughlin C,Puumala SE,Ross JA,Reynolds P

    更新日期:2011-06-01 00:00:00

  • Serum concentrations of squamous cell carcinoma antigen in patients with vulvar intraepithelial neoplasia and vulvar cancer.

    abstract::Our aim was to evaluate whether serum concentrations of squamous cell carcinoma antigen (SCC-Ag) are an independent prognostic factor in patients with vulvar cancer. We measured SCC-Ag in pretreatment serum samples of 55 patients with squamous cell vulvar cancer, 30 patients with vulvar intraepithelial neoplasia (VIN)...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hefler L,Obermair A,Tempfer C,van Houte M,Maenner G,Reinthaller A,Leodolter S,Kainz C

    更新日期:1999-06-21 00:00:00

  • Identification of a rhabdomyosarcoma targeting peptide by phage display with sequence similarities to the tumour lymphatic-homing peptide LyP-1.

    abstract::Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in children. To improve existing therapies and broaden the spectrum of cytotoxic agents that can be used in RMS treatment, we performed a phage-display-based screening for peptides that bind specifically to RMS cells. Two peptides binding to RMS and to othe...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Witt H,Hajdin K,Iljin K,Greiner O,Niggli FK,Schäfer BW,Bernasconi M

    更新日期:2009-05-01 00:00:00

  • Losses of the tumor suppressor BIN1 in breast carcinoma are frequent and reflect deficits in programmed cell death capacity.

    abstract::Oncogenic activation of MYC occurs often in breast carcinoma and is associated with poor prognosis. Loss or inactivation of mechanisms that restrain MYC may therefore be involved in tumor progression. In this study, we show that the MYC-interacting adaptor protein BIN1 is frequently missing in malignant breast cells a...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ge K,Duhadaway J,Sakamuro D,Wechsler-Reya R,Reynolds C,Prendergast GC

    更新日期:2000-02-01 00:00:00