Abstract:
:Failed fertilization after intracytoplasmic sperm injection (ICSI) can occur due to an oocyte activation defect. In these cases assisted oocyte activation (AOA) may help but efficiency is still unknown. Prior to AOA, the mouse oocyte activation test (MOAT) can be carried out by injecting human spermatozoa into mouse oocytes to evaluate their activating capacity. According to the MOAT activation percentage achieved, patients were classified into three groups: 0-20% (16 patients); 20-85% (seven patients); 85-100% (seven patients). For AOA, CaCl(2) was injected together with spermatozoa followed by a double Ca(2+) ionophore treatment. The fertilization rates before application of AOA in 50 cycles were 6%, 22% and 14% in, respectively, groups 1, 2 and 3 without any pregnancy. Fertilization and pregnancy rates after AOA in 61 cycles were significantly increased to 75% and 34% for group 1, 73% and 43% for group 2, and 75% and 17% for group 3 (P < 0.0001 and P < 0.004, respectively). Application of AOA results in normal fertilization and pregnancy rates in patients whose spermatozoa show deficient activation. When MOAT reveals no activation deficiency in spermatozoa, AOA still allows for high fertilization and acceptable pregnancy rates. The obstetric and neonatal outcomes after AOA were normal as no malformations were observed.
journal_name
Reprod Biomed Onlinejournal_title
Reproductive biomedicine onlineauthors
Heindryckx B,De Gheselle S,Gerris J,Dhont M,De Sutter Pdoi
10.1016/s1472-6483(10)60313-6subject
Has Abstractpub_date
2008-11-01 00:00:00pages
662-8issue
5eissn
1472-6483issn
1472-6491pii
S1472-6483(10)60313-6journal_volume
17pub_type
杂志文章abstract::Polycystic ovary syndrome (PCOS) is a complex and heterogeneous disease that involves menstrual dysfunction and reproductive difficulty, as well as metabolic problems. The aim of this study was to assess the effectiveness of myo-inositol (MYO) and d-chiro-inositol (DCI) on improving oocyte or embryo quality and pregna...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.rbmo.2017.07.005
更新日期:2017-11-01 00:00:00
abstract::The predictive value of pre-ovulatory hormones for achievement of pregnancy has been extensively evaluated in assisted reproductive technologies. Currently, no high-quality evidence exists to support or deny an association between oestradiol levels on the day of human chorionic gonadotrophin (HCG) administration and p...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章,评审
doi:10.1016/s1472-6483(10)60447-6
更新日期:2009-01-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2016.06.005
更新日期:2016-09-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/s1472-6483(10)60223-4
更新日期:2008-09-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::This study investigated the effects of long-term (24h) in-vitro sperm incubation at room temperature (RT; 23°C) versus testis temperature (35°C) on various sperm-quality parameters. Semen samples (n=41) were prepared both by density-gradient centrifugation (DGC) and the swim-up technique in order to compare the influe...
journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2013.12.005
更新日期:2014-04-01 00:00:00
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journal_title:Reproductive biomedicine online
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更新日期:2019-07-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2018.11.034
更新日期:2019-06-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2014.01.018
更新日期:2014-06-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章,评审
doi:10.1016/s1472-6483(10)62016-0
更新日期:2006-07-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2018.10.007
更新日期:2019-02-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/s1472-6483(10)61591-x
更新日期:2002-07-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章,评审
doi:10.1016/s1472-6483(12)60121-7
更新日期:2002-01-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章,随机对照试验
doi:10.1016/s1472-6483(10)60466-x
更新日期:2008-04-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章,评审
doi:10.1016/s1472-6483(10)60530-5
更新日期:2007-12-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2019.06.014
更新日期:2019-11-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2010.12.005
更新日期:2011-04-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2009.06.003
更新日期:2009-10-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
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更新日期:2010-06-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章,评审
doi:10.1016/s1472-6483(10)61182-0
更新日期:2006-05-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章,随机对照试验
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更新日期:2017-08-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2012.03.002
更新日期:2012-06-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
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更新日期:2014-02-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
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更新日期:2019-08-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2012.01.013
更新日期:2012-06-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2017.10.107
更新日期:2018-01-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.rbmo.2011.06.015
更新日期:2011-10-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type:
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更新日期:2008-02-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/j.rbmo.2011.03.009
更新日期:2011-06-01 00:00:00
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journal_title:Reproductive biomedicine online
pub_type: 杂志文章
doi:10.1016/s1472-6483(10)61284-9
更新日期:2004-10-01 00:00:00