当前位置： SCI文献检索 > EUROPEAN UROLOGY期刊下所有文献 > Hydrogen sulphide is involved in testosterone vascular effect.

Hydrogen sulphide is involved in testosterone vascular effect.

Abstract：

BACKGROUND:Testosterone (T) induces a rapid relaxation in vascular tissues of different species due to a nongenomic effect of this steroid on vessels. Different mechanisms have been proposed to explain T-induced vasodilatation but the effective mechanism(s) and the mediators involved are still a matter of debate. OBJECTIVES:We have evaluated if H(2)S pathway is involved in T vascular effects. DESIGN AND SETTING:Male Wistar rats were sacrificed and thoracic aorta was rapidly dissected and cleaned from fat and connective tissue. Rings of 2-3 mm length were cut and placed in organ baths filled with oxygenated Krebs solution at 37 degrees C and mounted to isometric force transducers. H(2)S determination was performed on thoracic aortic rings incubated with T or vehicle and in presence of inhibitors. H2S concentration was calculated against a calibration curve of NaHS (3-250 microM). Results were expressed as nmoles/mg protein. MEASUREMENTS:Vascular reactivity was evaluated by using isometric transducers. H(2)S determination was performed by using a cystathionine beta-synthetase (CBS) and cystathionine gamma lyase (CSE) activity assay. CSE and CBS protein levels were assessed by Western blot analysis. Statistical analysis was performed by using two-way ANOVA and unpaired Student's t-test where appropriate. RESULTS:T significantly increased conversion of L-cysteine to H(2)S. This effect was significantly reduced by PGG and BCA, two specific inhibitors of CSE. T (10 nM-10 microM) induced a concentration-dependent vasodilatation of rat aortic rings in vitro that was significantly and concentration-dependent inhibited by PGG, BCA, and glybenclamide. Incubation of aorta with T up to 1 h did not change CBS/CSE expression, suggesting that T modulates enzymatic activity. CONCLUSIONS:Here we demonstrate that T vasodilator effect involves H(2)S, a novel gaseous mediator. T modulates H(2)S levels by increasing the enzymatic conversion of L-cysteine to H(2)S.

journal_name

Eur Urol

journal_title

European urology

authors

Bucci M,Mirone V,Di Lorenzo A,Vellecco V,Roviezzo F,Brancaleone V,Ciro I,Cirino G

doi

10.1016/j.eururo.2008.05.014

subject

Has Abstract

pub_date

2009-08-01 00:00:00

pages

378-83

issue

eissn

0302-2838

issn

1873-7560

pii

S0302-2838(08)00621-0

journal_volume

pub_type

杂志文章

在线工具

Hydrogen sulphide is involved in testosterone vascular effect.