Abstract:
AIMS:To compare the sensitivity and specificity of new rabbit monoclonal antibody SP3 with those of mouse monoclonal and rabbit polyclonal antibodies using HER2 amplification defined by chromogenic in situ hybridisation (CISH) as the gold standard. METHODS:Serial sections from tissue microarrays (TMAs) containing 84 breast carcinomas were submitted to CISH (Zymed HER2 Spot-Light kit) and immunohistochemistry, using NeoMarkers SP3 (rabbit monoclonal), DAKO A0485 and DAKO HercepTest (polyclonal), Novocastra NCL-CB11, Cell Marque CM-CB11, and Genentech 4D5 (mouse monoclonal). RESULTS:The best antibody concordance was between SP3 and HercepTest (kappa = 0.74). SP3, A0485 and HercepTest detected all HER2 amplified tumours, but were less specific than mouse monoclonal antibodies. 3/38 (7.9%) and 8/38 (21.0%) non-amplified tumours were scored as 3+ using SP3 and A0485, respectively. 3/46 (6.5%) amplified tumours were negative for NCL-CB11. SP3, HercepTest and A0485 showed no gene amplification on 55%, 62.5% and 92.3% of the 2+ scored tumours, but most of the 2+ scored tumours using monoclonal antibodies were amplified by CISH (80-92.3%). CONCLUSIONS:SP3 is more sensitive than mouse monoclonal antibodies for Her2 assessment. However, HercepTest, CB11 and 4D5 show higher specificity than SP3 for the identification of HER2 gene amplification. Mouse monoclonal antibodies show less Her2 2+ tumours; most are amplified by CISH.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Nunes CB,Rocha RM,Reis-Filho JS,Lambros MB,Rocha GF,Sanches FS,Oliveira FN,Gobbi Hdoi
10.1136/jcp.2007.053892subject
Has Abstractpub_date
2008-08-01 00:00:00pages
934-8issue
8eissn
0021-9746issn
1472-4146pii
jcp.2007.053892journal_volume
61pub_type
杂志文章abstract::The sensitivity of an indirect immunofluorescence antibody test (IFAT) and a rapid microagglutination test (RMAT) for the diagnosis of Legionnaires' disease caused by Legionella pneumophila serogroup 1 was evaluated using serum specimens from 119 patients with bacteriologically confirmed infections. The sensitivity of...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.40.1.77
更新日期:1987-01-01 00:00:00
abstract::A quantitative method of estimating heparin co-factor in terms of percentage activity is presented. The activity in sera from 75 normal subjects varied little from 100%. Low results were obtained in sera from 50 cases of varying types of malignancy. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.16.2.115
更新日期:1963-03-01 00:00:00
abstract::Idoxuridine-treated McCoy cells grown as monolayers in 96 well microplates provide a convenient method for the isolation of Chlamydia trachomatis. Staining of infected monolayers with periodic acid-Schiff reagent (PAS) allows easy recognition of C trachomatis inclusions without the need for dark-ground microscopy. By ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.7.712
更新日期:1981-07-01 00:00:00
abstract::Sperm-agglutinating antibodies were present in three (2-6%) of 117 fertile men and in 19 (33-9%) of 56 men who had been vasectomized. Twenty-four of the 56 vasectomized men had been studied before vasectomy; sperm-agglutinating antibodies were present in one (4-3%) compared with eight (33-3%) after vasectomy. No sperm...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.29.12.1127
更新日期:1976-12-01 00:00:00
abstract::Methods are described for the estimation of plasma and urine alpha-amino nitrogen by the colorimetric ninhydrin reaction; in the method for urine, both ammonia and urea are separated by a rapid ion-exchange resin procedure. Two methods of estimation are given; one has the advantage of great simplicity, but involves th...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.17.2.150
更新日期:1964-03-01 00:00:00
abstract::Foveolar dysplasia is an uncommon form of dysplasia that is encountered in the stomach and oesophagus in the context of Barrett’s oesophagus. Glands displaying foveolar dysplasia also show architectural abnormalities that are similar to those encountered in adenomatous dysplasia. However, from a cytological point of v...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jclinpath-2014-202600
更新日期:2014-10-01 00:00:00
abstract::Group-specific polysaccharides of Neisseria meningitidis groups A and C have been demonstrated by means of a rapid, sensitive slide agglutination test with latex particles coated with antibodies. In this manner, the diagnosis can be made from the cerebrospinal fluid of patients with meningococcal meningitis caused by ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.25.12.1079
更新日期:1972-12-01 00:00:00
abstract::Antibodies against the group polysaccharide of group A streptococci were estimated by means of a haemagglutination reaction. In this reaction human erythrocytes of blood group O were sensitized with polysaccharide esterified with myristoylchloride. The optimal conditions of the reactions were determined by varying the...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.27.11.891
更新日期:1974-11-01 00:00:00
abstract:AIMS:To compare the efficacy of and tolerance to oral fluconazole and intraconazole in preventing fungal infection in neutropenic patients with haematological malignancies. PATIENTS:213 consecutive, afebrile adult patients treated with or without autologous stem cell transplantation for haematological malignancies. M...
journal_title:Journal of clinical pathology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1136/jcp.52.5.376
更新日期:1999-05-01 00:00:00
abstract::During each of the four-year periods 1971-1975 and 1975-1979, the annual incidence of hepatitis B infection has been assessed in 56 patients with haemophilia A by measuring plasma HBsAg, anti-HBs and anti-HBc levels. Infection rates of 7% and 9.5% per annum respectively were observed for each four-year period despite ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.5.577
更新日期:1983-05-01 00:00:00
abstract::This 14th best practice review is the second of a pair that examines tumour marker requesting primary care situations. This review considers carbohydrate antigen 125, α-fetoprotein and human chorionic gonadotropin. It is presented in question-answer format, referenced for each question. The recommendations represent a...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jclinpath-2011-200293
更新日期:2012-02-01 00:00:00
abstract:OBJECTIVES:Intraduct papillary mucinous neoplasm (IPMN) is a pancreatic tumour that is often associated with chronic pancreatitis (CP) in the surrounding pancreas. Type 1 autoimmune pancreatitis (AIP) is a fibro-inflammatory condition with characteristic histological features and prominent IgG4+ plasma cells and is par...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2013-201516
更新日期:2013-08-01 00:00:00
abstract::Metabolic balance studies for iron have been carried out on 24 apparently healthy elderly people (11 men and 13 women) aged 69.7 to 85.5 years living in their own homes and eating self selected diets. Several biochemical and haematological indices of iron state were also measured. The mean daily iron intake was 176 mu...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.37.12.1353
更新日期:1984-12-01 00:00:00
abstract::Antibiotic discs from Oxoid, Mast, AB Biodisk, Difco, and Baltimore Biological Laboratories were compared, where discs of similar antibiotic content were available, in diffusion-sensitivity tests against organisms of known sensitivity. Discs from Oxoid and Mast gave zones 1-5 mm larger than discs from other manufactur...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.28.10.779
更新日期:1975-10-01 00:00:00
abstract:AIMS:To study the localisation of the endothelial nitric oxide synthase (eNOS) in the normal placenta, with special emphasis on the implantation site in the first trimester of pregnancy, and in the different subtypes of trophoblastic cells in gestational trophoblastic disease. METHODS:The immunoperoxidase technique wi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.51.6.427
更新日期:1998-06-01 00:00:00
abstract:BACKGROUND:Malignant deciduoid mesothelioma is a rare variant of epithelioid mesothelioma. This tumour generally has poor prognosis, and can be asbestos related. AIM:To identify peculiar genetic changes responsible for critical phases in pathogenesis of malignant deciduoid mesothelioma and their prognostic relevance. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.026435
更新日期:2006-07-01 00:00:00
abstract::Histological examination of the lower margin of the internal sphincter has demonstrated an increased amount of fibrous tissue in patients with haemorrhoids compared with controls. This finding may be of relevance to the treatment of haemorrhoids by maximal anal dilatation. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.31.3.268
更新日期:1978-03-01 00:00:00
abstract::Cancer lineage/tissue-of-origin assignment in cancers of unknown primary remains a challenge even when aided by massively parallel sequencing. The stakes are high for patients as many contemporary therapeutic strategies are disease-specific, and the biological differences can influence the patients' responses. Herein,...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2020-206895
更新日期:2020-08-27 00:00:00
abstract::The calcium, vitamin D, and osteocalcin concentrations were investigated in 17 patients with anorexia nervosa. Serum 25-hydroxyvitamin D (25 OHD) concentrations below normal were observed in 15 (88%); only two patients has serum 1,25 dihydroxycholecalciferol (1,25(OH)2D) concentrations below normal. Serum parathyroid ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.41.2.195
更新日期:1988-02-01 00:00:00
abstract::Guinea-pigs, rats, dogs, rabbits, monkeys, sheep, and goats were screened for G-6PD activity in erythrocytes by the methaemoglobin reduction test, the brilliant cresyl blue decolorization test, and the spectrophotometric determination of the NADPH generation rate. Guinea-pigs, rats, dogs, rabbits, and monkeys were fou...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.19.6.614
更新日期:1966-11-01 00:00:00
abstract::Gastrointestinal cell proliferation plays an important role in the maintenance of the integrity of the gastrointestinal system. The study of gastrointestinal proliferation kinetics allows a better understanding of the complexity of the system, and also has important implications for the study of gastrointestinal carci...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.52.5.321
更新日期:1999-05-01 00:00:00
abstract::Eight strains of Moraxella phenylpyruvica have been isolated from clinical material in the United Kingdom, the first to be reported from this country. They were characterized, together with three strains of M. phenylpyruvica of the National Collection of Type Cultures (NCTC), and compared with NCTC strains of eight ot...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.25.11.959
更新日期:1972-11-01 00:00:00
abstract::A middle aged man developed very high fever, status epilepticus, and terminal acute renal failure with myoglobinuria after surgery. A post mortem examination showed widespread muscle necrosis with hypercontraction bands. Muscle enzyme studies and electron microscopic examination disclosed central core disease, a condi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.45.4.361
更新日期:1992-04-01 00:00:00
abstract::A case of a 20 year old Japanese woman who developed thyroid cancer exhibiting unusual cribriform structures while being followed up for familial adenomatous polyposis/Gardner's syndrome is reported. The patient presented with osteomas, pigmented retinal lesions, and adenomas of the duodenum and the papilla of Vater, ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.49.7.611
更新日期:1996-07-01 00:00:00
abstract::A simple, cheap and readily available test system for biological safety cabinets is described. It depends on the containment of an aerosol of Bacillus subtilis spores generated in a BIRD micronebulizer and the measurement of air flows with an anemometer. The system was set up to survey new equipment but equally valuab...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.27.7.585
更新日期:1974-07-01 00:00:00
abstract:AIMS:This study was aimed to investigate the role of erythrocyte, platelet and reticulocyte (RET) parameters, measured by new haematological analyser Sysmex XN and C reactive protein (CRP), for early diagnosis of sepsis during intensive care unit (ICU) stay. METHODS:The study population consisted of 62 ICU patients, 2...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2017-204643
更新日期:2018-04-01 00:00:00
abstract::Candida spp are the fourth most common group of nosocomial pathogens isolated from patients on medical, surgical, and intensive care wards. Polymicrobial candidaemia has rarely been described. The diagnosis of candidaemia from peripheral blood smears has not been widely reported. This report describes the case of a yo...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2003.9340
更新日期:2004-02-01 00:00:00
abstract:AIM:To determine the size of a cervical biopsy specimen with human papillomavirus (HPV) infection required to enable in situ hybridisation to be carried out with a guarantee of a reliable result. METHODS:In situ hybridisation was carried out in 142 cervical uterine biopsy specimens classified histologically as low gra...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.1.57
更新日期:1995-01-01 00:00:00
abstract::Serum lysozyme levels were determined by a turbidometric method using egg white lysozyme as standard in 100 patients with Crohn's disease, 86 with ulcerative colitis, 31 with coeliac disease, and in 38 normal control subjects. Though the levels in Crohn's disease were significantly higher than those in ulcerative coli...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.29.7.598
更新日期:1976-07-01 00:00:00
abstract::Of 30 children with bleeding disorders exposed to an index tuberculosis case, six developed primary pulmonary tuberculosis and two others became positive skin reactors. There was a significant correlation between the amount of replacement treatment received and the development of evidence of tuberculous infection. Onl...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.10.1163
更新日期:1985-10-01 00:00:00