Abstract：
:Systematic protein expression studies in the brain of exercising and sedentary animals have not been carried out so far and it was therefore decided to determine differences in metabolic protein levels in rat hippocampus of sedentary, voluntary and involuntary exercising rats by a proteomic approach. Aged, male Sprague-Dawley rats, 23 months old, were used for the study: the first group consisted of sedentary rats, the second of rats with voluntary exercise from five to 23 months and the third group was performing involuntary exercise on a treadmill from five to 23 months. Two-dimensional gel electrophoresis with subsequent mass spectrometrical identification of spots followed by quantification of spots was carried out. Identification of significantly differential proteins was validated by the determination of the corresponding enzyme activity. Five individual metabolic proteins showed differential protein levels in the three groups: mitochondrial precursors of ornithine aminotransferase, isocitrate dehydrogenase [NAD] subunit alpha, malate dehydrogenase, ubiquinol-cytochrome-c reductase complex core protein 1, and ubiquitin carboxyl-terminal hydrolase isozyme L1. The unambiguously identified metabolic proteins were mainly of mitochondrial localization and fit the expectations of altered mitochondrial activity in exercise. Reduced ubiquitin carboxyl-terminal hydrolase isoenzyme L1 levels in treadmill (forced) exercise show the involvement of the proteasomal pathway as a novel finding. These results not only form the basis for functional studies elucidating mechanisms and differences between voluntary and forced exercise in hippocampal metabolism but also highlight the most intriguing aspect that exercise is affecting the brain at the protein level.
journal_name
Exp Neuroljournal_title
Experimental neurologyauthors
Kirchner L,Chen WQ,Afjehi-Sadat L,Viidik A,Skalicky M,Höger H,Lubec Gdoi
10.1016/j.expneurol.2008.03.014subject
Has Abstractpub_date
2008-07-01 00:00:00pages
145-51issue
1eissn
0014-4886issn
1090-2430pii
S0014-4886(08)00119-2journal_volume
212pub_type
杂志文章abstract：:It is not yet clear how L-dopa, that is the most effective drug for the treatment of Parkinson's disease, enters into the dopaminergic neurons to be transformed into dopamine. It is suggested that L-dopa is mainly transported into cells by a group of L-amino acid carriers named "System L". Since these carriers are sel...
journal_title：Experimental neurology
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journal_title：Experimental neurology
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pub_type： 杂志文章
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journal_title：Experimental neurology
pub_type： 杂志文章
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更新日期：2010-11-01 00:00:00
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journal_title：Experimental neurology
pub_type： 杂志文章
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journal_title：Experimental neurology
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journal_title：Experimental neurology
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journal_title：Experimental neurology
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pub_type： 杂志文章
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journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1999.7037
更新日期：1999-05-01 00:00:00
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journal_title：Experimental neurology
pub_type： 杂志文章
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更新日期：2003-08-01 00:00:00
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journal_title：Experimental neurology
pub_type： 杂志文章
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更新日期：1987-03-01 00:00:00
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journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.2002.7989
更新日期：2002-09-01 00:00:00
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journal_title：Experimental neurology
pub_type： 杂志文章
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更新日期：2010-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.expneurol.2009.04.007
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journal_title：Experimental neurology
pub_type： 杂志文章
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journal_title：Experimental neurology
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pub_type： 杂志文章,评审
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pub_type： 杂志文章
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journal_title：Experimental neurology
pub_type： 杂志文章
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更新日期：2004-09-01 00:00:00
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journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1997.6647
更新日期：1997-11-01 00:00:00
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journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1996.0004
更新日期：1996-01-01 00:00:00
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journal_title：Experimental neurology
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journal_title：Experimental neurology
pub_type： 杂志文章,评审
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更新日期：2010-09-01 00:00:00
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journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(89)90040-x
更新日期：1989-06-01 00:00:00
