Abstract：
:A new method is presented for the quantification of cell viability based on densitometry with computerized image analysis. Neuronal cells were stained with crystal violet and densitometric analysis was performed with an IBAS 2.0 image analyzer (Kontron/ Zeiss), using specially implemented dedicated software which integrates the optical density of the culture in each well with the area covered by the stained cells. To test the reliability of the densitometric method cortical cells were plated at different concentrations (5 x 10(4)-10(6)/ml); the standard curve obtained by analysis of crystal violet staining showed a linear proportion between cell number and optical density signal. The validation and accuracy of the method were assessed and compared with other methods using rat cortical cells cultured in vitro for 10 days and exposed to kainic acid (250 microM) for 24 h. Neuronal viability was reduced by 40-50% and comparison with direct cell counting, MTT assay, and spectrophotometric analysis confirmed that the method is simple, quick, and reliable.
journal_name
Exp Neuroljournal_title
Experimental neurologyauthors
Andreoni G,Angeretti N,Lucca E,Forloni Gdoi
10.1006/exnr.1997.6647subject
Has Abstractpub_date
1997-11-01 00:00:00pages
281-7issue
1eissn
0014-4886issn
1090-2430pii
S0014-4886(97)96647-4journal_volume
148pub_type
杂志文章abstract：:Axons are the anatomical link between neuronal cell bodies and their target organs, and thus axonal degeneration is the pathological substrate that underlies neurological dysfunction in a large number of neurological conditions. Recent advances in the field of axonal biology demonstrate that axons possess programs for...
journal_title：Experimental neurology
pub_type： 杂志文章,评审
doi：10.1016/j.expneurol.2013.05.001
更新日期：2013-08-01 00:00:00
abstract：:When antibodies specific to fast-twitch, slow-twitch, and slow-tonic myosins were used to stain the clasp muscle, m. sternoradialis, of Xenopus laevis, three predominant fiber types were identified in both males and females. Three fiber types can also be distinguished by the diameters and conduction velocities of thei...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(83)90414-4
更新日期：1983-11-01 00:00:00
abstract：:Neuronal populations projecting to a common target may compete for neurotrophic substances. To determine if competition impairs target reinnervation, we examined the effect of capsaicin-induced sensory denervation on sympathetic nerve ingrowth to the sympathectomized rat superior tarsal smooth muscle. In tarsal muscle...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(92)90025-l
更新日期：1992-10-01 00:00:00
abstract：:Wistar rats (n = 58) were injected subcutaneously during the lactation period with fluoxetine (5, 10, 20 or 40 mg/kg/day) and cortical spreading depression (SD) was recorded immediately after weaning (25-30 days of life). An additional group (10 mg/kg; n = 8) was SD-recorded at 60-70 days. As compared to the saline-in...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2006.02.014
更新日期：2006-08-01 00:00:00
abstract：:Levodopa or short-acting dopamine (DA) agonist treatment of advanced parkinsonian patients exposes striatal DA receptors to non-physiologic intermittent stimulation that contributes to the development of dyskinesias and other motor complications. To determine whether continuous dopaminergic stimulation can delay or pr...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2004.11.013
更新日期：2005-03-01 00:00:00
abstract：:Neurons from cerebral neocortex and hippocampus exhibit a striking difference in vulnerability to transient global ischemia. In order to study the contribution of neuronal connections and neuron-glia interactions to this variation in neuronal vulnerability, we used hippocampal and neocortical cultures submitted to var...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1997.6728
更新日期：1998-03-01 00:00:00
abstract：:Temporomandibular disorders (TMDs) are orofacial pains within the trigeminal distribution, which involve the masticatory musculature, the temporomandibular joint or both. Their pathophysiology remains unclear, as inflammatory mediators are thought to be involved, and clinically TMD presents pain and sometimes limitati...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2015.05.005
更新日期：2015-09-01 00:00:00
abstract：:Spinal cord injury results in cell loss, disruption of neural circuitry and chronic functional impairment. Several different cell types generate progeny in response to injury, which participate in scar formation and remyelination. Work over the last few years has identified neural stem cells and delineated the stem ce...
journal_title：Experimental neurology
pub_type： 杂志文章,评审
doi：10.1016/j.expneurol.2013.01.026
更新日期：2014-10-01 00:00:00
abstract：:Electrical stimulation of the trigeminal ganglion has been widely used as a model of nociception, characterizing migraine. This treatment is known to evoke release of neuropeptides and neurotransmitters from nerve fibers of the dura mater. On the basis of immunocytochemical investigations, we found that under normal c...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2003.12.001
更新日期：2004-04-01 00:00:00
abstract：:Astrocytes are relatively resistant to injury compared to neurons and oligodendrocytes. Here, we report transient region-specific loss of astrocytes in mice early after pilocarpine-induced status epilepticus (SE). In the dentate hilus, immunoreactivity for glial acidic fibrillary protein (GFAP) was decreased, and the ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2006.04.031
更新日期：2006-10-01 00:00:00
abstract：:Outcome following stroke is difficult to measure because the behavioral response to infarction is variable. We hypothesized that cognitive function, such as spatial learning, may be a reproducible and sensitive outcome variable. We developed an animal model of multifocal cerebral ischemia in order to study the effects...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(92)90160-r
更新日期：1992-05-01 00:00:00
abstract：BACKGROUND:Intracerebral hemorrhage (ICH) is the common brain diseases in middle-aged and elderly people, with high disability and/or mortality rate, and is a serious public health concern. Both WNK3 kinase and the WNK3/SPAK/NKCC1 signaling pathway play an integral role in maintaining normal cell homeostasis. However, ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2020.113386
更新日期：2020-10-01 00:00:00
abstract：:Rat cortical explants were cultured in the presence or absence of the mitotic inhibitor cytosine arabinoside to determine whether or not it affects the composition of the nonneuronal cell population within the outgrowth zone. Ultrastructural morphometric analysis of the incidence of fibroblasts, fibrous astrocytes, an...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(86)90092-0
更新日期：1986-05-01 00:00:00
abstract：:HIV-associated neurocognitive disorders (HAND) continue to persist despite effective control of viral replication. Although the mechanisms underlying HAND are poorly understood, recent attention has focused on altered neuronal population activity as a correlate of impaired cognition. However, while alterations in neur...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2019.113077
更新日期：2020-01-01 00:00:00
abstract：:We have previously found that fetal cortex taken from 16- to 18-day-old donors survives grafting to the infarcted cortex 5-7 days after middle cerebral artery occlusion. The present study was undertaken to examine the effect on graft survival of varying the age of the fetal donor tissue and the time between vessel occ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1994.1086
更新日期：1994-05-01 00:00:00
abstract：:Traumatic axonal injury (TAI) evolves within minutes to hours following traumatic brain injury (TBI). Previous studies have identified axolemmal disruption and impaired axonal transport (AxT) as key mechanisms in the evolution of TAI. While initially hypothesized that axolemmal disruption culminates in impaired AxT, p...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2004.05.022
更新日期：2004-11-01 00:00:00
abstract：:Recent evidence indicates that protein aggregation and in particular the formation of toxic protein oligomers is a key mechanism in synucleinopathies such as Parkinson's disease (PD). Post mortem brain tissue studies as well as animal studies furthermore suggest that matrix metalloproteinases (MMPs) are also involved ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2008.10.010
更新日期：2009-01-01 00:00:00
abstract：:Neuropathic pain is common after traumatic injuries to the cauda equina/conus medullaris and brachial plexus. Clinically, this pain is difficult to treat and its mechanisms are not well understood. Lesions to the ventral roots are common in these injuries, but are rarely considered as potential contributors to pain. W...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2006.11.003
更新日期：2007-03-01 00:00:00
abstract：:Mitochondria play a pivotal role in the development of pathology associated with Parkinson's disease (PD), traumatic brain injury (TBI), and following exposure to the environmental toxin trichloroethylene (TCE). Evidence from humans indicates that both TBI and TCE can play a role in the development of PD and that each...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2011.12.012
更新日期：2012-03-01 00:00:00
abstract：:SCI is followed by dramatic upregulation of chondroitin sulfate proteoglycans (CSPGs) which limit axonal regeneration, oligodendrocyte replacement and remyelination. The recent discovery of the specific CSPGs signaling receptor protein tyrosine phosphatase sigma (RPTPσ) provided an opportunity to refine the therapeuti...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2018.08.003
更新日期：2018-11-01 00:00:00
abstract：:Ghrelin has been shown to regulate neurogenesis in the hippocampus. The aim of this study was to investigate the possible influence of ghrelin on cell proliferation and neuroblast formation in the subventricular zone (SVZ) and rostral migratory system (RMS) and generation of interneurons in the olfactory bulb (OB). We...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2013.11.021
更新日期：2014-02-01 00:00:00
abstract：:Spectral analysis is a mathematical method which contains and generalizes frequency analysis. The form of spectral analysis most applicable to EEG is tutorially presented, using both algebraic formulae and four simplified illustrative examples. The examples are each converted into an autocorrelogram and auto-spectrogr...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(63)90042-6
更新日期：1963-08-01 00:00:00
abstract：:Nerve injury frequently results in development of chronic, dysesthetic pain and allodynia (painful sensation in response to benign stimulation). Following nerve injury, spinal cord glia become activated and secrete a number of inflammatory cytokines, including interleukin-1 (IL-1), which exists as two genetically dist...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2009.03.022
更新日期：2009-06-01 00:00:00
abstract：:In a model of Parkinson's disease (PD), amphetamine, a dopamine (DA)-releasing drug, fails to induce ipsilateral drug rotations in a proportion of rats with complete unilateral 6-hydroxydopamine (6-OHDA) lesions of the medial forebrain bundle and DA neurons of the substantia nigra. To investigate this phenomenon, indi...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.2001.7823
更新日期：2001-12-01 00:00:00
abstract：:Purkinje neurons are a sensitive and specialised cell type important for fine motor movement and coordination. Purkinje cell damage manifests as motor incoordination and ataxia - a prominent feature of many human disorders including spinocerebellar ataxia and Huntington's disease. A correlation between Purkinje degene...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2013.08.001
更新日期：2013-11-01 00:00:00
abstract：:Olfactory ensheathing cells (OEC) constitute a specialized population of glia that accompany primary olfactory axons and have been reported to facilitate axonal regeneration after spinal cord injury in vivo. In the present report we describe OEC neurotrophic factor expression and neurotrophic properties of OECs in vit...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/s0014-4886(02)00058-4
更新日期：2003-04-01 00:00:00
abstract：:Transplantation of genetically engineered cells can provide sustained focal delivery of naturally occurring molecules, including neurotransmitters and growth factors. We have engineered immortalized mouse cortical neurons and glia to deliver GABA by driving GAD(65) expression. Engineered cell lines showed GAD(65) mRNA...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1999.7305
更新日期：2000-02-01 00:00:00
abstract：:It is controversial whether levodopa (L-DOPA) therapy in the early stages of Parkinson's disease (PD) predisposes to later complications. Partial lesions of the substantia nigra (SN) in rat provide a model of the early stages of PD. We have investigated the potential effects of L-DOPA in early PD by analyzing its infl...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1993.1057
更新日期：1993-04-01 00:00:00
abstract：:To ascertain whether signaling due to peripheral inflammation affects motoneuron vulnerability, we examined in adult rats the reaction to axonal injury of facial motoneurons primed by muscle inflammation. In this double-hit paradigm, preconditioning was achieved by injections into the facial muscles of the T cell mito...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.2002.7908
更新日期：2002-07-01 00:00:00
abstract：:Falls are a major source of hospitalization, long-term institutionalization, and death in older adults and patients with Parkinson's disease (PD). Limited attentional resources are a major risk factor for falls. In this review, we specify cognitive-behavioral mechanisms that produce falls and map these mechanisms onto...
journal_title：Experimental neurology
pub_type： 杂志文章,评审
doi：10.1016/j.expneurol.2014.04.032
更新日期：2014-07-01 00:00:00
