Changes in mitochondrial gene and protein expression in grass shrimp, Palaemonetes pugio, exposed to chronic hypoxia.


:Spatial and temporal increases of hypoxia in estuaries are of major environmental concern. Since mitochondria consume most of the oxygen in the cell, we examined the potential role of mitochondrial gene and protein expression in adaptation to chronic hypoxia in the grass shrimp Palaemonetes pugio. Grass shrimp were exposed to DO levels slightly above and below the critical pO(2), 1.8 mg/L, for P. pugio, and hypoxia-induced alterations in gene expression were screened using custom cDNA macroarrays. Mitochondrial gene expression was not affected by exposure to moderate hypoxia (2.5mg/L DO). However, chronic exposure to severe hypoxia (1.5mg/L DO) for 7 days resulted in an increase of transcription of genes present in the mitochondrial genome (including 16S rRNA and Ccox 1), together with up-regulation of genes involved in Fe/heme metabolism. This pattern was completely reversed by day 14, when a significant down-regulation of these genes was observed. Separating mitochondrial proteins in two dimensions by IEF and reverse phase chromatography, followed by LC/MS/MS of differentially expressed proteins, showed cytochrome c oxidase subunit 2, encoded by Ccox 2, was down-regulated after 12d exposure to severe hypoxia. It appears therefore that decreases in mitochondrial Ccox gene transcription result in decreased mitochondrial Ccox protein synthesis. These results suggest that mitochondrial genes and proteins show promise as molecular indicators of exposure to hypoxia.


Mar Environ Res


Brouwer M,Brown-Peterson NJ,Hoexum-Brouwer T,Manning S,Denslow N




Has Abstract


2008-07-01 00:00:00














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    pub_type: 杂志文章


    authors: Greenstein D,Bay S,Jirik A,Brown J,Alexander C

    更新日期:2003-07-01 00:00:00

  • Sea urchin larvae show resilience to ocean acidification at the time of settlement and metamorphosis.

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    journal_title:Marine environmental research

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    authors: Espinel-Velasco N,Agüera A,Lamare M

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  • Induction of vitellogenin in vivo and in vitro in the model teleost medaka (Oryzias latipes): comparison of gene expression and protein levels.

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    journal_title:Marine environmental research

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    authors: Scholz S,Kordes C,Hamann J,Gutzeit HO

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  • Effects of benzo[a]pyrene on DNA damage and histological alterations in gonad of scallop Chlamys farreri.

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    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Jing-Jing M,Lu-qing P,Jing L,Lin Z

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    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Pastore AS,Santacroce MP,Narracci M,Cavallo RA,Acquaviva MI,Casalino E,Colamonaco M,Crescenzo G

    更新日期:2014-09-01 00:00:00

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    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Almroth BC,Sturve J,Stephensen E,Holth TF,Förlin L

    更新日期:2008-08-01 00:00:00

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    pub_type: 杂志文章


    authors: Cabrita MT,Brito P,Caçador I,Duarte B

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    pub_type: 杂志文章


    authors: Burt J,Al-Harthi S,Al-Cibahy A

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    journal_title:Marine environmental research

    pub_type: 杂志文章,评审


    authors: Beyer J,Green NW,Brooks S,Allan IJ,Ruus A,Gomes T,Bråte ILN,Schøyen M

    更新日期:2017-09-01 00:00:00

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    authors: Ruus A,Hjermann DØ,Beylich B,Schøyen M,Øxnevad S,Green NW

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    authors: Moreland JJ,Arrieta DE,Washburn BS

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    journal_title:Marine environmental research

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    authors: Elberling B,Knudsen KL,Kristensen PH,Asmund G

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    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Boitsov S,Petrova V,Jensen HK,Kursheva A,Litvinenko I,Klungsøyr J

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Coelho MR,Bebianno MJ,Langston WJ

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    authors: Reopanichkul P,Carter RW,Worachananant S,Crossland CJ

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    authors: Broeg K,Kaiser W,Bahns S,Koehler A

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    journal_title:Marine environmental research

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    authors: Manyin T,Rowe CL

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  • Trace element bioaccumulation in reef fish from New Caledonia: influence of trophic groups and risk assessment for consumers.

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    authors: Metian M,Warnau M,Chouvelon T,Pedraza F,Rodriguez y Baena AM,Bustamante P

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Laurent MC,Gros O,Brulport JP,Gaill F,Bris NL

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  • Cetacean response to summer maritime traffic in the Western Mediterranean Sea.

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    pub_type: 杂志文章


    authors: Campana I,Crosti R,Angeletti D,Carosso L,David L,Di-Méglio N,Moulins A,Rosso M,Tepsich P,Arcangeli A

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    pub_type: 杂志文章


    authors: Faksness LG,Altin D,Størseth TR,Nordtug T,Hansen BH

    更新日期:2020-05-01 00:00:00