Abstract:
:Vibrio parahaemolyticus is a leading cause of seafood-borne gastroenteritis; however, its virulence mechanisms are not well understood. The identification of type III secreted proteins has provided candidate virulence factors whose functions are still being elucidated. Genotypic strain variability contributes a level of complexity to understanding the role of different virulence factors. The ability of V. parahaemolyticus to inhibit Rho family GTPases and cause cytoskeletal disruption was examined with HeLa cells. After HeLa cells were infected, intracellular Rho activation was inhibited in response to external stimuli. In vitro activation of Rho, Rac, and Cdc42 isolated from infected HeLa cell lysates was also inhibited, indicating that the bacteria were specifically targeting GTPase activation. The inhibition of Rho family GTPase activation was retained for clinical and environmental isolates of V. parahaemolyticus and was dependent on a functional chromosome I type III secretion system (CI-T3SS). GTPase inhibition was independent of hemolytic toxin genotype and the chromasome II (CII)-T3SS. Rho inhibition was accompanied by a shift in the total actin pool to its monomeric form. These phenotypes were abrogated in a mutant strain lacking the CI-T3S effector Vp1686, suggesting that the inhibiting actin polymerization may be a downstream effect of Vp1686-dependent GTPase inhibition. Although Vp1686 has been previously characterized as a potential virulence factor in macrophages, our findings reveal an effect on cultured HeLa cells. The ability to inhibit Rho family GTPases independently of the CII-T3SS and the hemolytic toxins may provide insight into the mechanisms of virulence used by strains lacking these virulence factors.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Casselli T,Lynch T,Southward CM,Jones BW,DeVinney Rdoi
10.1128/IAI.01704-07subject
Has Abstractpub_date
2008-05-01 00:00:00pages
2202-11issue
5eissn
0019-9567issn
1098-5522pii
IAI.01704-07journal_volume
76pub_type
杂志文章abstract::An attenuated Salmonella typhi Ty21a vaccine was administered to 18 infants and toddlers (less than or equal to 24 months old) to determine its safety and immunogenicity. The vaccination (10(9) CFU per dose, three doses) was well tolerated. However, after the vaccination there was no evidence of a humoral or cellular ...
journal_title:Infection and immunity
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1128/IAI.59.11.4291-4293.1991
更新日期:1991-11-01 00:00:00
abstract::Exposure of mononuclear cells from the mouse peritoneal cavity to interferon (IF)-containing mouse sera enhanced phagocytosis of colloidal carbon particles by the cells. The same effect was observed when the cells were exposed to IF-containing cell culture harvest free of serum. The magnitude of this effect of IF-cont...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.5.581-588.1971
更新日期:1971-11-01 00:00:00
abstract::Salmonella is a widespread zoonotic enteropathogen that causes gastroenteritis and fatal typhoidal disease in mammals. During systemic infection of mice, Salmonella enterica serovar Typhimurium resides and replicates in macrophages within the "Salmonella-containing vacuole" (SCV). It is surprising that the substrates ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00093-09
更新日期:2009-07-01 00:00:00
abstract::Development of an effective vaccine against Leishmania infection is a priority of tropical disease research. We have recently demonstrated protection against Leishmania major in the murine and nonhuman primate models with individual or combinations of purified leishmanial recombinant antigens delivered as plasmid DNA ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.8.4215-4225.2002
更新日期:2002-08-01 00:00:00
abstract::When inoculated orally, Escherichia coli strain 1056 caused acute enteritis in 7 of 22 weanling hamsters. E. coli strain 1056 was isolated from the ileum of a hamster with proliferative ileitis. It was lactose negative, nonmotile, and anaerogenic. By electron microscopy and indirect fluorescent-antibody techniques, E....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.3.1232-1238.1981
更新日期:1981-03-01 00:00:00
abstract::The mechanism of complement-mediated killing of seven serum-sensitive Pseudomonas aeruginosa strains was examined. All seven strains were sensitive to the bactericidal activity of 20% pooled normal human serum (PNHS) containing magnesium EGTA, which blocks the classical complement pathway (CCP), or 20% PNHS preheated ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.3.632-639.1988
更新日期:1988-03-01 00:00:00
abstract::The intravenous administration of hydrogen peroxide has been reported to benefit patients with pneumonia and to reduce Plasmodium parasitemia in experimentally infected mice. We assessed the antibacterial activity of intravenously infused hydrogen peroxide against hydrogen peroxide-susceptible Escherichia coli (MBC of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.3.607-610.1985
更新日期:1985-06-01 00:00:00
abstract::Bacillary angiomatosis (BA), one of the many clinical manifestations resulting from infection with the facultative intracellular bacterium Bartonella henselae, is characterized by angiogenic lesions. Macrophages have been identified as important effector cells contributing to the angiogenic process during B. henselae ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.9.5735-5742.2005
更新日期:2005-09-01 00:00:00
abstract::A group of insertion mutants was used to define the genes of plasmid pDC5 required for the expression of mannose-resistant fimbriae. Minicell experiments identified four polypeptides (71,000, 45,000, 27,000, and 17,000 daltons) concerned with fimbrial production, the smallest of these being the fimbrial subunit. The a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.3.900-906.1983
更新日期:1983-12-01 00:00:00
abstract::Neisseria gonorrhoeae, the etiologic agent of gonorrhea, is frequently asymptomatic in women, often leading to chronic infections. One factor contributing to this may be biofilm formation. N. gonorrhoeae can form biofilms on glass and plastic surfaces. There is also evidence that biofilm formation may occur during nat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00036-09
更新日期:2009-09-01 00:00:00
abstract::Borrelia burgdorferi produces a mitogen for murine B lymphocytes which can be measured in vitro by polyclonal stimulation of proliferation and immunoglobulin production (R. Schoenfeld, B. Araneo, Y. Ma, L. Yang, and J. J. Weis, Infect. Immun. 60:455-464, 1992). Sonicated B. burgdorferi cells also stimulated IL-6 produ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.8.3033-3041.1992
更新日期:1992-08-01 00:00:00
abstract::Incubation of INT407 cells with various clinical isolates of Campylobacter jejuni resulted in secretion of interleukin-8 (IL-8) at levels ranging from 96 to 554 pg/ml at 24 h. The strains which produced the highest levels of IL-8 secretion were 81-176 and BT44. Induction of IL-8 secretion required live cells of 81-176...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.1.88-93.1999
更新日期:1999-01-01 00:00:00
abstract::One mechanism by which interferons (IFNs) can inhibit chlamydial infection is by the induction of the enzyme indoleamine 2,3-dioxygenase (IDO), which restricts the availability of tryptophan, which is required for chlamydial growth. Other immunomodulating agents, including interleukin-1 (IL-1), can interact synergisti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.5.1870-1875.1995
更新日期:1995-05-01 00:00:00
abstract::In previous studies, we reported that fibronectin (FN) markedly enhances phagocytic uptake of antibody-coated group B streptococci (GBS) by human polymorphonuclear leukocytes. Furthermore, administration of FN along with a GBS type-specific monoclonal or polyclonal antibody to infected neonatal rats significantly enha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.6.2334-2339.1993
更新日期:1993-06-01 00:00:00
abstract::The gonococcal pilus is a primary virulence factor, providing the initial attachment of the bacterial cell to human mucosal tissues. Pilin, the major subunit of the pilus, can carry a wide spectrum of primary amino acid sequences which are generated by the action of a complex antigenic variation system. Changes in the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.5.1918-1927.1998
更新日期:1998-05-01 00:00:00
abstract::Humans are the sole reservoir of Neisseria gonorrhoeae, an organism which undergoes a marked increase in metabolic rate after exposure to a low-molecular-weight, heat-stable component(s) of human serum. Further studies on the effect of serum on gonococcal metabolism were undertaken. Gonococcal broth (GCB) is commonly ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.3.738-744.1985
更新日期:1985-12-01 00:00:00
abstract::Immunization with avirulent Salmonella typhimurium strain SL3235, a smooth, aroA- derivative, was shown to induce high levels of resistance to challenge with virulent S. typhimurium in innately hypersusceptible C3H/HeJ mice and inherently resistant C3H/HeNCrlBR mice. Strain SL3235 is one of a class of avirulent aroA- ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.3.605-612.1985
更新日期:1985-03-01 00:00:00
abstract::Shiga toxin type 1 (Stx1) belongs to the Shiga family of bipartite AB toxins that inactivate eukaryotic 60S ribosomes. The A subunit of Stxs are N-glycosidases that share structural and functional features in their catalytic center and in an internal hydrophobic region that shows strong transmembrane propensity. Both ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.11.5252-5259.1998
更新日期:1998-11-01 00:00:00
abstract::Cytolethal distending toxins (CDT) constitute an emerging heterogeneous family of bacterial toxins whose common biological property is to inhibit the proliferation of cells in culture by blocking their cycle at G2/M phase. In this study, we investigated the molecular mechanisms underlying the block caused by CDT from ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.12.5088-5095.1997
更新日期:1997-12-01 00:00:00
abstract::The route of pathogen inoculation by needle has been shown to influence the outcome of infection. Employing needle inoculation of the obligately intracellular parasite Leishmania major, which is transmitted in nature following intradermal (i.d.) deposition of parasites by the bite of an infected sand fly, we identifie...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01600-13
更新日期:2014-07-01 00:00:00
abstract::The mucosal humoral immune response elicited following Shigella flexneri infection in patients living in Antananarivo districts (Madagascar Island) was evaluated by measuring the gut-derived, circulating immunoglobulin A (IgA) antibody-secreting cells (ASC) specific for the major bacterial antigen lipopolysaccharide (...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.69.9.5230-5234.2001
更新日期:2001-09-01 00:00:00
abstract::Two weeks after mice had been infected in the footpad with cells of Nocardia brasiliensis, immunoglobulin and C3 deposits were observed in tissue from the inoculation site. Both immunoglobulin and C3 were found in the actinomycotic granules that characterize the mycetoma and in the surrounding inflammatory zones, in w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.3.1218-1222.1983
更新日期:1983-06-01 00:00:00
abstract::Smoking is associated with increased susceptibility to tuberculosis and influenza. However, little information is available on the mechanisms underlying this increased susceptibility. Mice were left unexposed or were exposed to cigarette smoke and then infected with Mycobacterium tuberculosis by aerosol or influenza A...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00709-10
更新日期:2011-01-01 00:00:00
abstract::Cells of the marine fish pathogen Vibrio anguillarum 775 harboring a plasmid associated with virulence can grow unaffected in the presence of iron-binding compounds such as transferrin. In contrast, the growth of isogenic plasmidless derivatives is inhibited by the presence of iron chelators. Radioactive from (55Fe3+)...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.1.223-227.1981
更新日期:1981-01-01 00:00:00
abstract::We have performed microarray hybridization studies on 40 clinical isolates from 12 common serovars within Salmonella enterica subspecies I to identify the conserved chromosomal gene pool. We were able to separate the core invariant portion of the genome by a novel mathematical approach using a decision tree based on g...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.12.7894-7905.2005
更新日期:2005-12-01 00:00:00
abstract::ExoU is a potent effector protein that causes rapid host cell death upon injection by the type III secretion system of Pseudomonas aeruginosa. The N-terminal half of ExoU contains a patatin-like phospholipase A(2) (PLA(2)) domain that requires the host cell cofactor superoxide dismutase 1 (SOD1) for activation, while ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00223-10
更新日期:2010-08-01 00:00:00
abstract::Changes in cell viability and in factors affecting metabolic integrity were examined after exposure of Escherichia coli LP1092 to human serum. Antibody-dependent classical pathway activity accounted for the rapid killing of strain LP1092 by complement. Removal of serum lysozyme by bentonite absorption or by neutraliza...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.1.122-131.1983
更新日期:1983-01-01 00:00:00
abstract::The P8 proteoglycolipid complex (P8 PGLC) is a glyconjugate expressed by Leishmania mexicana complex parasites. We previously have shown that vaccination with P8 PGLC provides protection against cutaneous leishmaniasis in susceptible BALB/c mice. However, the biological importance of this complex remains unknown. Here...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01528-07
更新日期:2008-05-01 00:00:00
abstract::The data presented indicate that in experimental Pseudomonas aeruginosa infection of mice, protease enhances the virulence of the organism. Anesthetized CBA/Lü mice were subjected to a 15-s flame burn and infected with a wild-type protease-producing strain and two of its protease-deficient mutants. The average bacteri...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.1.181-187.1979
更新日期:1979-04-01 00:00:00
abstract::Most children and adults living in areas where the endemicity of Plasmodium falciparum malaria is high have significantly elevated levels of both total immunoglobulin E (IgE) and IgE antimalarial antibodies in blood. This elevation is highest in patients with cerebral malaria, suggesting a pathogenic role for this imm...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.1.116-121.1997
更新日期:1997-01-01 00:00:00