Dermal matrix as a carrier for in vivo delivery of human adipose-derived stem cells.


:The aim of the present study was to evaluate the potential of acellular dermal matrix as a carrier for delivery of stem cells to the site of soft tissue defect in a murine skin injury model and to determine the potential of stem cells delivered via such an approach to successfully engraft, survive and differentiate locally. We showed that adipose-derived stem cells delivered via this matrix survived after in vivo engraftment, spontaneously differentiated along vascular endothelial, fibroblastic and epidermal epithelial lineages and significantly improved wound healing. Furthermore, an organ survey for transplanted cells showed no evidence of a systemic distribution beyond the cutaneous wound site, indicating that the adipose-derived stem cell-dermal matrix construct provides a novel and effective method for anatomically focused cellular therapy. In conclusion, stem cell-seeded dermal matrix is an effective means for targeted in vivo cell delivery for enhanced soft tissue regeneration.






Altman AM,Matthias N,Yan Y,Song YH,Bai X,Chiu ES,Slakey DP,Alt EU




Has Abstract


2008-04-01 00:00:00














  • Development of a two-part biomaterial adhesive strategy for annulus fibrosus repair and ex vivo evaluation of implant herniation risk.

    abstract::Intervertebral disc (IVD) herniation causes pain and disability, but current discectomy procedures alleviate pain without repairing annulus fibrosus (AF) defects. Tissue engineering strategies seal AF defects by utilizing hydrogel systems to prevent recurrent herniation, however current biomaterials are limited by poo...


    pub_type: 杂志文章


    authors: DiStefano TJ,Shmukler JO,Danias G,Di Pauli von Treuheim T,Hom WW,Goldberg DA,Laudier DM,Nasser PR,Hecht AC,Nicoll SB,Iatridis JC

    更新日期:2020-11-01 00:00:00

  • Long-term stability in vivo of a thromboresistant heparinized surface.

    abstract::A surface with covalently bonded heparin was tested in an in vivo model. Heparinized and non-heparinized tubings were inserted in the pig aorta. They were left in place for up to 16 wk. After removal they were analysed for their capacity to take up and inhibit thrombin. A comparison was also made with tubings that had...


    pub_type: 杂志文章


    authors: Arnander C,Bagger-Sjöbäck D,Frebelius S,Larsson R,Swedenborg J

    更新日期:1987-11-01 00:00:00

  • The effect of surface coating on the bond strength of machinable ceramics.

    abstract::This study examined the nature of the surface presented by a machinable feldspathic porcelain and by a machinable glass-ceramic for bonding to conventional composite luting agents and measured the bond strengths which may be achieved with various surface treatments. There was no significant difference between the bond...


    pub_type: 杂志文章


    authors: Jedynakiewicz NM,Martin N

    更新日期:2001-04-01 00:00:00

  • Reversible opening of the blood-brain barrier by claudin-5-binding variants of Clostridium perfringens enterotoxin's claudin-binding domain.

    abstract::The blood-brain barrier (BBB) prevents entry of neurotoxic substances but also that of drugs into the brain. Here, the paracellular barrier is formed by tight junctions (TJs) with claudin-5 (Cldn5) being the main sealing constituent. Transient BBB opening by targeting Cldn5 could improve paracellular drug delivery. Th...


    pub_type: 杂志文章


    authors: Neuhaus W,Piontek A,Protze J,Eichner M,Mahringer A,Subileau EA,Lee IM,Schulzke JD,Krause G,Piontek J

    更新日期:2018-04-01 00:00:00

  • Ceria nanocrystals decorated mesoporous silica nanoparticle based ROS-scavenging tissue adhesive for highly efficient regenerative wound healing.

    abstract::Restoration of tissue integrity and tissue function of wounded skin are both essential for wound repair and regeneration, while synergistic promotion of the two remains elusive. Since elevated reactive oxygen species (ROS) production in the injured site has been implicated in triggering a set of deleterious effects su...


    pub_type: 杂志文章


    authors: Wu H,Li F,Wang S,Lu J,Li J,Du Y,Sun X,Chen X,Gao J,Ling D

    更新日期:2018-01-01 00:00:00

  • BMP10 as a potent inducer of trophoblast differentiation in human embryonic and induced pluripotent stem cells.

    abstract::Bone morphogenetic proteins (BMPs) are known to induce diverse differentiation fates in human embryonic stem cells (hESCs). In the present study, we compared the potency at which BMP5, BMP10 and BMP13, which are members of distinct BMP subgroups due to differences in sequential and structural homology, induce differen...


    pub_type: 杂志文章


    authors: Lichtner B,Knaus P,Lehrach H,Adjaye J

    更新日期:2013-12-01 00:00:00

  • Prodrug-embedded angiogenic vessel-targeting nanoparticle: A positive feedback amplifier in hypoxia-induced chemo-photo therapy.

    abstract::Photodynamic therapy (PDT) induced hypoxia can significantly upregulate the expression of vascular endothelial growth factor (VEGF) at the tumor-stromal interface, resulting in a promoted angiogenesis. Thus, an angiogenesis vessel-targeting nanoparticle (AVT-NP) consisting of photosensitizer, angiogenic vessel-targeti...


    pub_type: 杂志文章


    authors: Guo D,Xu S,Wang N,Jiang H,Huang Y,Jin X,Xue B,Zhang C,Zhu X

    更新日期:2017-11-01 00:00:00

  • Autonomous assembly of ordered metastable DNA nanoarchitecture and in situ visualizing of intracellular microRNAs.

    abstract::Facile assembly of intelligent DNA nanoobjects with the ability to exert in situ visualization of intracellular microRNAs (miRNAs) has long been concerned in the fields of DNA nanotechnology and basic medical study. Here, we present a driving primer (DP)-triggered polymerization-mediated metastable assembly (PMA) stra...


    pub_type: 杂志文章


    authors: Xu J,Wu ZS,Wang Z,Le J,Zheng T,Jia L

    更新日期:2017-03-01 00:00:00

  • Rat costochondral cell characteristics on poly (L-lactide-co-epsilon-caprolactone) scaffolds.

    abstract::This study was designed to examine the adhesion, proliferation, and morphology of chondrocytes on new scaffolds; and to examine these cells histologically for the ability of the chondrocytes to maintain chondrogenic properties after subcutaneous implantation into nude mice. Both 75:25 poly (L-lactide-co-epsilon-caprol...


    pub_type: 杂志文章


    authors: Honda M,Morikawa N,Hata K,Yada T,Morita S,Ueda M,Kimata K

    更新日期:2003-09-01 00:00:00

  • Trilayer micelles for combination delivery of rapamycin and siRNA targeting Y-box binding protein-1 (siYB-1).

    abstract::A three layer (trilayer) polymeric micelle system based on the self-association of the triblock polymer poly(ethylene glycol)-b-poly{N-[N-(2-aminoethyl)-2-aminoethyl] aspartamide}-b-poly(ε-caprolactone) (PEG-b-PAsp(DET)-b-PCL) has been synthesized and investigated for combination delivery of rapamycin (RAP) and siRNA ...


    pub_type: 杂志文章


    authors: Zeng S,Xiong MP

    更新日期:2013-09-01 00:00:00

  • Porous collagen-apatite nanocomposite foams as bone regeneration scaffolds.

    abstract::We have created a porous bioresorbable nanocomposite bone scaffold that chemically, structurally and mechanically matched natural bone so that it could be recognized and remodeled by natural bone. Containing collagen fibers and synthetic apatite nanocrystals, our scaffold has high strength for supporting the surroundi...


    pub_type: 杂志文章


    authors: Pek YS,Gao S,Arshad MS,Leck KJ,Ying JY

    更新日期:2008-11-01 00:00:00

  • Investigation of surface properties of some polymers by a thermodynamic and mechanical approach: possibility of predicting mucoadhesion and biocompatibility.

    abstract::Mucoadhesive properties of several polymers, such as sodium alginate, hydroxypropylmethyl cellulose, scleroglucan, xanthan gum, polyacrylic acid (Carbopol), and poly co-(methyl vinyl ether-maleic anhydride) (Gantrez), have been investigated by comparing a thermodynamical and a mechanical approach. Surface properties o...


    pub_type: 杂志文章


    authors: Esposito P,Colombo I,Lovrecich M

    更新日期:1994-02-01 00:00:00

  • Cell recruitment and transfection in gene activated collagen matrix.

    abstract::Realization of systems able to both recruit cells and influence their fate (affecting their processes) represents a new approach for tissue regeneration. We investigated the potency of gene activated matrix (GAM) and implemented the GAM strategy in order to achieve a control of gene expression, as well as a specific c...


    pub_type: 杂志文章


    authors: Orsi S,De Capua A,Guarnieri D,Marasco D,Netti PA

    更新日期:2010-01-01 00:00:00

  • Selective dissolution of AuCu and AuAg alloys in vitro and in vivo.

    abstract::AuCu and AuAg alloys (30 at. % Au) have been immersed in aqueous solutions of chloride and sulphide with additions of protein (mucin). The surfaces of the alloys were subsequently analysed by means of ESCA and compared with previous and similar tests without addition of mucin. No noticeable effect of the mucin additio...


    pub_type: 杂志文章


    authors: Herø H,Hultquist G,Odén A

    更新日期:1985-11-01 00:00:00

  • Ultraviolet light crosslinking of poly(trimethylene carbonate) for elastomeric tissue engineering scaffolds.

    abstract::A practical method of photocrosslinking high molecular weight poly(trimethylene carbonate)(PTMC) is presented. Flexible, elastomeric and biodegradable networks could be readily prepared by UV irradiating PTMC films containing pentaerythritol triacrylate (PETA) and a photoinitiator. The network characteristics, mechani...


    pub_type: 杂志文章


    authors: Bat E,Kothman BH,Higuera GA,van Blitterswijk CA,Feijen J,Grijpma DW

    更新日期:2010-11-01 00:00:00

  • Influence of nanoporous alumina membranes on long-term osteoblast response.

    abstract::A major goal of bone tissue engineering is to design better scaffold configuration and materials to better control osteoblast behavior. Nanoporous architecture has been shown to significantly affect cellular response. In this work, nanoporous alumina membranes were fabricated by a two-step anodization method to invest...


    pub_type: 杂志文章


    authors: Popat KC,Leary Swan EE,Mukhatyar V,Chatvanichkul KI,Mor GK,Grimes CA,Desai TA

    更新日期:2005-08-01 00:00:00

  • The isolation and differentiation of human adipose-derived stem cells using membrane filtration.

    abstract::Human adipose-derived stem cells (hADSCs) were purified from a suspension of human adipose tissue cells (stromal vascular fraction) by the conventional culture method and by membrane filtration through polyurethane (PU) foam membranes. hADSCs can be obtained from a suspension of human adipose tissue cells using the me...


    pub_type: 杂志文章


    authors: Wu CH,Lee FK,Suresh Kumar S,Ling QD,Chang Y,Chang Y,Wang HC,Chen H,Chen DC,Hsu ST,Higuchi A

    更新日期:2012-11-01 00:00:00

  • Coating of VEGF-releasing scaffolds with bioactive glass for angiogenesis and bone regeneration.

    abstract::Bioactive glasses are potentially useful as bone defect fillers, and vascular endothelial growth factor (VEGF) has demonstrated benefit in bone regeneration as well. We hypothesized that the specific combination of prolonged localized VEGF presentation from a matrix coated with a bioactive glass may enhance bone regen...


    pub_type: 杂志文章


    authors: Leach JK,Kaigler D,Wang Z,Krebsbach PH,Mooney DJ

    更新日期:2006-06-01 00:00:00

  • Temperature sensitive contact lenses for triggered ophthalmic drug delivery.

    abstract::Ophthalmic drug delivery through eye drops is inefficient because of low corneal bioavailability and short residence time in tears. Contact lenses are ideally suited for extended and targeted drug delivery to cornea, but commercial contact lenses release ophthalmic drugs for only 1-2 h. This study focuses on dispersin...


    pub_type: 杂志文章


    authors: Jung HJ,Chauhan A

    更新日期:2012-03-01 00:00:00

  • When stem cells meet graphene: Opportunities and challenges in regenerative medicine.

    abstract::Recent advances in stem cell research and nanotechnology have significantly influenced the landscape of tissue engineering and regenerative medicine. Precise and reproducible control of the fate of stem cells and their lineage specification have, therefore, become more crucial than ever for the success of stem cell-ba...


    pub_type: 杂志文章,评审


    authors: Kenry,Lee WC,Loh KP,Lim CT

    更新日期:2018-02-01 00:00:00

  • Effect of oligonucleotide mediated immobilization of bone morphogenic proteins on titanium surfaces.

    abstract::The aim of the present study was to test the hypothesis that oligonucleotides can be used for anchorage and slow release of osteogenic growth factors such as BMP to enhance the osteogenic activity of a titanium implant surface. Strands of 60-mer non-coding DNA oligonucleotides (ODN) were bound to an acid-etched sandbl...


    pub_type: 杂志文章


    authors: Schliephake H,Bötel C,Förster A,Schwenzer B,Reichert J,Scharnweber D

    更新日期:2012-02-01 00:00:00

  • Injectable, scalable 3D tissue-engineered model of marrow hematopoiesis.

    abstract::Modeling the interaction between the supportive stroma and the hematopoietic stem and progenitor cells (HSPC) is of high interest in the regeneration of the bone marrow niche in blood disorders. In this work, we present an injectable co-culture system to study this interaction in a coherent in vitro culture and in viv...


    pub_type: 杂志文章


    authors: Tavakol DN,Tratwal J,Bonini F,Genta M,Campos V,Burch P,Hoehnel S,Béduer A,Alessandrini M,Naveiras O,Braschler T

    更新日期:2020-02-01 00:00:00

  • An improvement in sintering property of beta-tricalcium phosphate by addition of calcium pyrophosphate.

    abstract::The sintering behavior of calcium pyrophosphate (CPP, Ca2P2O7)-doped beta-tricalcium phosphate [TCP, Ca3(PO4)2], prepared by solid state reaction, was investigated in-situ, using dilatometry. Pure beta-TCP undergoes phase transition to alpha-TCP at about 1200 degrees C; hence pure beta-TCP ceramics should be sintered ...


    pub_type: 杂志文章


    authors: Ryu HS,Youn HJ,Hong KS,Chang BS,Lee CK,Chung SS

    更新日期:2002-02-01 00:00:00

  • Enhancement of PDGF-BB mitogenic activity on human dermal fibroblasts by biospecific dextran derivatives.

    abstract::Dextran derivatives are biosynthetic polyanionic polymers which exert some of the heparin properties such as regulating the activity of several heparin-binding growth factors. Based on a reproducible synthetic procedure, we have synthesized a new generation of dextran derivatives named dextran methylcarboxylate benzyl...


    pub_type: 杂志文章


    authors: Vercoutter-Edouart AS,Dubreucq G,Vanhoecke B,Rigaut C,Renaux F,Dahri-Correia L,Lemoine J,Bracke M,Michalski JC,Correia J

    更新日期:2008-05-01 00:00:00

  • Mutual effect of subcutaneously transplanted human adipose-derived stem cells and pancreatic islets within fibrin gel.

    abstract::While subcutaneous tissue has been proposed as a potential site for pancreatic islet transplantation, concern remains that the microvasculature of subcutaneous tissue is too poor to support transplanted islets. In an effort to overcome this limitation, we evaluated whether fibrin gel with human adipose-derived stem ce...


    pub_type: 杂志文章


    authors: Bhang SH,Jung MJ,Shin JY,La WG,Hwang YH,Kim MJ,Kim BS,Lee DY

    更新日期:2013-10-01 00:00:00

  • Porous polymer implant for repair of meniscal lesions: a preliminary study in dogs.

    abstract::Artificial meniscal lesions extending into the avascular part of the meniscus, which do not heal by any other means, were repaired by suturing either a porous polymer implant or a synovial flap into the defect. The implant guided the ingrowth of vascular repair tissue into the defect. This fibrous tissue later on tran...


    pub_type: 杂志文章


    authors: Klompmaker J,Jansen HW,Veth RP,de Groot JH,Nijenhuis AJ,Pennings AJ

    更新日期:1991-11-01 00:00:00

  • Tissue engineering scaffolds using superstructures.

    abstract::Here, scaffolds as cell and tissue carriers are approached from an engineering point of view, emphasizing material superstructuring in the design of supports. Superstructure engineering provides optimal spatial and nutritional conditions for cell maintenance by the arrangement of structural elements (e.g. pores or fib...


    pub_type: 杂志文章


    authors: Wintermantel E,Mayer J,Blum J,Eckert KL,Lüscher P,Mathey M

    更新日期:1996-01-01 00:00:00

  • Mesenchymal stem cell-derived extracellular vesicles and retinal ischemia-reperfusion.

    abstract::Retinal ischemia is a major cause of vision loss and impairment and a common underlying mechanism associated with diseases such as glaucoma, diabetic retinopathy, and central retinal artery occlusion. The regenerative capacity of the diseased human retina is limited. Our previous studies have shown the neuroprotective...


    pub_type: 杂志文章


    authors: Mathew B,Ravindran S,Liu X,Torres L,Chennakesavalu M,Huang CC,Feng L,Zelka R,Lopez J,Sharma M,Roth S

    更新日期:2019-03-01 00:00:00

  • Phase transformation of plasma-sprayed hydroxyapatite coating with preferred crystalline orientation.

    abstract::Highly oriented hydroxyapatite coatings (HACs) were obtained on titanium substrates through a radio-frequency thermal plasma spraying (TPS) method. XRD patterns showed that the HACs had crystallites with [001] preferred orientation vertical to the coating's surface. XRD results also indicated that tetracalcium phospha...


    pub_type: 杂志文章


    authors: Inagaki M,Kameyama T

    更新日期:2007-07-01 00:00:00

  • Biologic response to passive dissolution of titanium craniofacial microplates.

    abstract::The effect of anodization on passive dissolution of titanium was studied by measuring titanium levels in peritoneal leukocytes and tissues of laboratory animals with titanium plates implanted into the peritoneal cavity. Fifteen Sprague-Dawley rats were assigned randomly to three treatment groups of five animals. One g...


    pub_type: 杂志文章


    authors: Jorgenson DS,Centeno JA,Mayer MH,Topper MJ,Nossov PC,Mullick FG,Manson PN

    更新日期:1999-04-01 00:00:00