Abstract：
:Cell-extracellular cell matrix (ECM) interactions play a vital role in development including cytoskeletal structure, growth and differentiation. A 15 amino acid, non-RGD containing, peptide that is derived from type I collagen was tested for its ability to confer cell survival. Two routinely utilized bone graft materials, anorganic bone matrix (ABM) and ABM carrying the cell binding peptide P-15 were compared for cell viability and apoptosis. Anchorage-dependent human foreskin fibroblasts (HFF) or osteogenic MC3T3-E1 cells were seeded on ABM or ABM/P-15. After serum withdrawal, viability and level of apoptosis were significantly (p<0.05) improved for cells on ABM/P-15 compared to cells on ABM. Scanning electron microscopy confirmed large, spread cells on ABM/P-15 and smaller cells with apoptotic cellular blebs on ABM. In addition, viable cell attachment was significantly greater on cells cultured on ABM/P-15 compared with demineralized freeze-dried bone allograft. This is the first report that the P-15 cell binding peptide, when complexed with ABM, promotes cell survival. The P-15 peptide may modulate cell number and tissue structure by the enhancement of viable cell attachment and via the regulation of apoptosis.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Hanks T,Atkinson BLdoi
10.1016/j.biomaterials.2003.12.007keywords:
subject
Has Abstractpub_date
2004-08-01 00:00:00pages
4831-6issue
19eissn
0142-9612issn
1878-5905pii
S0142961203011529journal_volume
25pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract：:An ideal scaffold that provides a combination of suitable mechanical properties along with biological signals is required for successful ligament/tendon regeneration in mesenchymal stem cell-based tissue engineering strategies. Among the various fibre-based scaffolds that have been used, hybrid fibrous scaffolds compr...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2010.01.004
更新日期：2010-04-01 00:00:00
abstract：:We report here the development of a chitosan/beta-glycerophosphate(Ch/beta-GP) thermo-sensitive gel to deliver ellagic acid (EA) for cancer treatment. The properties of the Ch/beta-GP gels were characterized regarding chemical structure, surface morphology, and viscoelasticity. In vitro EA release rate from the EA loa...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2010.01.139
更新日期：2010-05-01 00:00:00
abstract：:The effects of phosphorylated chitin (P-chitin) on the tissue responses to two kinds of calcium phosphate cements (CPCs) were investigated using experimental rabbits. One of them consisting of monocalcium phosphate monohydrate, calcium oxide, 1 M phosphate buffer (pH: 7.4) and different amounts of P-chitin (CPC-I or P...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(02)00205-3
更新日期：2002-12-01 00:00:00
abstract：:Efficient and site-specific delivery of anticancer drugs to tumors is important in the development of effective cancer chemotherapy. As an undecapeptide of the tachykinin neuropeptide family, the substance P (SP)/neurokinin-1 receptor (NK1R) system has been identified as a promising ligand-receptor pair in tumor-speci...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2016.11.011
更新日期：2017-03-01 00:00:00
abstract：:Fibrosis affects most organs, it results in replacement of normal parenchymal tissue with collagen-rich extracellular matrix, which compromises tissue architecture and ultimately causes loss of function of the affected organ. Biochemical pathways that contribute to fibrosis have been extensively studied, but the role ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2014.07.048
更新日期：2014-12-01 00:00:00
abstract：:The continuous release of nitric oxide (NO) by the native endothelium of blood vessels plays a substantial role in the cardiovascular physiology, as it influences important pathways of cardiovascular homeostasis, inhibits vascular smooth muscle cell (VSMC) proliferation, inhibits platelet activation and aggregation, a...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2015.06.016
更新日期：2015-09-01 00:00:00
abstract：:A major obstacle in the survival and efficacy of tissue engineered transplants is inadequate oxygenation, whereby unsupportive oxygen tensions result in significant cellular dysfunction and death within the implant. In a previous report, we developed an innovative oxygen generating biomaterial, termed OxySite, to prov...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2017.03.018
更新日期：2017-06-01 00:00:00
abstract：:The potential for base poly(ethylene glycol) graft poly(acrylic acid) PEG-g-PA copolymers and surface-modified PEG-g-PA materials to inhibit random protein fouling and bacterial adhesion are investigated. PEG-g-PA co-polymers were synthesized that inhibited non-specific protein and cellular adhesion. PEG-g-PA co-polym...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2003.09.020
更新日期：2004-05-01 00:00:00
abstract：:The ligand-tethered poly(ethylene oxide-propylene oxide-ethylene oxide) (PEO-PPO-PEO) triblock copolymer was explored to engineer poly(DL-lactic acid) (PDL-LA) material to promote cell attachment and growth. The PEO-PPO-PEO was activated by methyl sulfonyl chloride and the amino acid, and peptide were attached. By ble...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2003.08.031
更新日期：2004-05-01 00:00:00
abstract：:The study is focused on the evaluation of the potential bioadhesive behaviour of chitosan and thiolated chitosan (chitosan-TBA)-coated poly(isobutyl cyanoacrylates) (PIBCA) nanoparticles. Nanoparticles were obtained by radical emulsion polymerisation with chitosan of different molecular weight and with different propo...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2007.01.005
更新日期：2007-04-01 00:00:00
abstract：:We investigated the hypothesis that immobilizing TGF-β1 within fibrin hydrogels may act in synergy with cyclic mechanical stimulation to enhance the properties of vascular grafts. To this end, we engineered a fusion TGF-β1 protein that can covalently anchor to fibrin during polymerization upon the action of factor XII...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2013.05.073
更新日期：2013-10-01 00:00:00
abstract：:The present work assesses the potential of three-dimensional porous titanium scaffolds produced by a novel powder metallurgy process for applications in bone engineering through in vitro experimentation. Mouse MC3T3-E1 pre-osteoblasts were used to investigate the proliferation (DNA content), differentiation (alkaline ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2005.05.046
更新日期：2005-12-01 00:00:00
abstract：:Valvular interstitial cells (VICs) actively maintain and repair heart valve tissue; however, persistent activation of VICs to a myofibroblast phenotype can lead to aortic stenosis. To better understand and quantify how microenvironmental cues influence VIC phenotype and myofibroblast activation, we compared expression...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2015.09.035
更新日期：2016-01-01 00:00:00
abstract：:Stimuli-responsive nanoparticles hold great promise for drug delivery to improve the safety and efficacy of cancer therapy. One of the most investigated stimuli-responsive strategies is to induce drug release by heating with laser, ultrasound, or electromagnetic field. More recently, cryosurgery (also called cryothera...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2018.07.021
更新日期：2018-10-01 00:00:00
abstract：:Sodium alginate hydrogel, stabilized with gelatin, is a suitable, biologically inert matrix that can be used for encapsulating and 3D bioprinting of bone-related SaOS-2 cells. However, the cells, embedded in this matrix, remain in a non-proliferating state. Here we show that addition of an overlay onto the bioprinted ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2014.07.002
更新日期：2014-10-01 00:00:00
abstract：:As synergistic photothermal immunotherapy has developed as one of the most attractive strategies for cancer therapy, it is crucial to design an effective photothermal immunotherapy system to enhance the synergistic anti-tumor effect and reveal the essential role of each treatment. In this study, we designed CpG self-c...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2019.04.024
更新日期：2019-07-01 00:00:00
abstract：:Adipose-derived stem cells (ASCs) have valuable applications in regenerative medicine, but maintaining the stemness of ASCs during in vitro culture is still a challenging issue. In this study, human ASCs spontaneously formed three-dimensional spheroids on chitosan films. Most ASCs within the spheroid were viable, and ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.11.049
更新日期：2012-02-01 00:00:00
abstract：:Eye drops producing long-acting ocular anesthesia would be desirable for corneal pain management. Here we present liposome-based formulations to achieve very long ocular anesthetic effect after a single eye drop instillation. The liposomes were functionalized with succinyl-Concanavalin A (sConA-Lip), which can bind co...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2018.07.054
更新日期：2018-10-01 00:00:00
abstract：:Resurfacing of cartilage defects using cell-seeded, biomaterial grafts is a promising approach for articular cartilage repair and in this study we investigated the ability of human chondrocytes from osteoarthritic joints to generate cartilage tissue under standard conditions in cultured over 21 days on Hyalograft matr...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2008.09.064
更新日期：2009-02-01 00:00:00
abstract：:A general solution of the diffusion equation for gas in polymers is developed by a numerical method. This solution allows diffusion to be calculated in all cases, by gas desorption after an incomplete adsorption, which is not the case for analytical solutions. When the analytical solutions are valid, both types of sol...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(87)90004-4
更新日期：1987-09-01 00:00:00
abstract：:This study was undertaken to investigate the relative importance of a hydroxyapatite (HA) coating and the macrotexture of titanium implants to the quality of bony ingrowth and fixation. Various types of titanium cylinders were implanted into the cancellous bone of the intercondylar region of the distal femur of the do...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(98)00011-8
更新日期：1999-01-01 00:00:00
abstract：:The limited intrinsic repair capacity of articular cartilage has stimulated continuing efforts to develop tissue engineered analogues. Matrices composed of type II collagen and chondroitin sulfate (CS), the major constituents of hyaline cartilage, may create an appropriate environment for the generation of cartilage-l...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(02)00067-4
更新日期：2002-08-01 00:00:00
abstract：:Three critical aspects of searching for and understanding how to find highly resistant surfaces to protein adhesion are addressed here with specific application to synthetic membrane filtration. They include the (i) discovery of a series of previously unreported monomers from a large library of monomers with high prot...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.11.001
更新日期：2012-02-01 00:00:00
abstract：:Using the material technique recently developed by us, we prepared a micropattern on poly(ethylene glycol) (PEG) hydrogel to keep background resistant to cell adhesion for a long time, which made examination of differentiation of localized stem cells available. Our micropattern designed in this paper prevented or ensu...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2009.12.006
更新日期：2010-03-01 00:00:00
abstract：:Water uptake characteristics of BisGMA-based composites incorporating untreated and surface-treated hydroxyapatite (HA) with a silane coupling agent have been investigated. The water absorption and desorption behaviour of these composites obeyed the classical diffusion theory. The diffusion coefficients of the composi...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(01)00331-3
更新日期：2002-04-01 00:00:00
abstract：:To fully understand biological behavior in vitro often dictates that oxygen be reported at either a local or a cellular level. Oxygen sensors based on the luminescent quenching of a specific form of electrospun fiber were developed for measurement of both gaseous and dissolved oxygen concentrations. Electrospinning wa...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2015.10.055
更新日期：2016-01-01 00:00:00
abstract：:Elucidation of relationship among cellular uptake, localization and biological activities of metal complexes could make great breakthrough in the understanding of their action mechanisms and provide useful information for rational design of metal-based anticancer drugs. Iron(II) complexes have emerged as potential ant...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2015.08.031
更新日期：2015-12-01 00:00:00
abstract：:For women diagnosed with leukemia, transplantation of cryopreserved ovarian tissue after disease remission is not advisable. Therefore, to restore fertility in these patients, we aim to develop a biodegradable artificial ovary that offers an environment where isolated follicles and ovarian cells (OCs) can survive and ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2012.05.015
更新日期：2012-09-01 00:00:00
abstract：:Docetaxel (DOC) produces anti-tumor effects by inducing apoptosis and inhibiting cell growth. However, its clinical application is limited by its hydrophobicity and low biocompatibility. Therefore, improving DOC's water solubility, biocompatibility, and anti-tumor effects are important goals that will improve its clin...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2015.02.108
更新日期：2015-01-01 00:00:00
abstract：:Interest in constructing a reliable 3-dimensional (3D) collagen culture platform in microfabricated systems is increasing as researchers strive to investigate reciprocal interaction between extracellular matrix (ECM) and cells under various conditions. However, in comparison to conventional 2-dimensional (2D) cell cul...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2009.05.043
更新日期：2009-09-01 00:00:00
