Oxidized low density lipoproteins induce a pathologic response by retinal pigmented epithelial cells.


:The accumulation of apolipoprotein B100 lipoproteins in Bruch membrane is an early event thought to promote age-related macular degeneration (AMD). Immunohistochemistry using an anti-oxidized low density lipoprotein antibody on 10 AMD specimens showed staining in Bruch membrane including basal deposits, a marker of AMD. To determine whether retinal pigmented epithelial cells develop a pathologic phenotype after interaction with lipoproteins, ARPE-19 cells were exposed to low density lipoproteins (LDL) or oxidized LDLs (oxLDL). Analysis using the Affymetrix U133 Plus 2.0 (Affymetrix, Inc., Santa Clara, CA, USA) gene chip showed physiological and pathological transcriptional responses after LDL and oxLDL treatment, respectively. LDL induced a down-regulation of cholesterol biosynthesis genes while oxLDL induced transcriptional alterations in genes related to lipid metabolism, oxidative stress, inflammation and apoptosis. Electrospray mass spectrometry showed that oxLDL, but not LDL induced large cellular increases of sphingomyelin, ceramides, and cholesteryl esters. With TUNEL labeling, oxLDL caused 14.6% apoptosis compared to <1% after LDL. Addition of an inhibitor of sphingomyelin synthase inhibited this apoptosis by 41%. These data support the hypothesis that oxidized lipoproteins are one trigger for initiating early events in the pathogenesis of AMD.


J Neurochem


Yamada Y,Tian J,Yang Y,Cutler RG,Wu T,Telljohann RS,Mattson MP,Handa JT




Has Abstract


2008-05-01 00:00:00














  • Muscarinic and nicotinic cholinergic binding sites in the terminal abdominal ganglion of the cricket (Acheta domesticus).

    abstract::Cricket (Acheta domesticus) terminal abdominal ganglia (TG) contain high concentrations (approximately 2 pmol/mg protein) of muscarinic and nicotinic cholinergic binding sites, based on the capacity of TG to bind specifically the labelled ligands L-[3H]quinuclidinyl benzilate ([3H]QNB) and [125I]alpha-bungarotoxin ([1...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Meyer MR,Reddy GR

    更新日期:1985-10-01 00:00:00

  • Trk is a calmodulin-binding protein: implications for receptor processing.

    abstract::The tyrosine kinase receptors for the neurotrophins (Trk) are a family of transmembrane receptors that regulate the differentiation and survival of different neuronal populations. Neurotrophin binding to Trk leads to the activation of several signalling pathways including a rapid, but moderate, increase in intracellul...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Llovera M,de Pablo Y,Egea J,Encinas M,Peiró S,Martín-Zanca D,Rocamora N,Comella JX

    更新日期:2004-01-01 00:00:00

  • Nuclear calmodulin-binding proteins in rat neurons.

    abstract::By using a 125I-calmodulin overlay assay, three major high-affinity calmodulin-binding proteins, showing apparent molecular masses of 135, 60, and 50 kDa, have been detected in purified nuclear fractions isolated from rat neurons. It has been shown that after extraction of the nuclei with nucleases and high salt, all ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Pujol MJ,Bosser R,Vendrell M,Serratosa J,Bachs O

    更新日期:1993-04-01 00:00:00

  • Phorbol ester-induced upregulation of angiotensin II receptors in neuronal cultures is potentiated by a calcium ionophore.

    abstract::Previous studies have suggested that protein kinase C is important in the regulation of angiotensin II receptors in neuronal cultures, because the C-kinase agonists, phorbol esters, are able to increase the number of these receptors. In the present study, we have further investigated the role of protein kinase C in an...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Sumners C,Rueth SM,Myers LM,Kalberg CJ,Crews FT,Raizada MK

    更新日期:1988-07-01 00:00:00

  • Characterization and comparison of neurofilament proteins from rat and mouse CNS.

    abstract::Rat and mouse CNS neurofilament proteins (NFPs) were characterized and compared, in terms of electrophoretic properties on polyacrylamide gels and by peptide mapping, with one another and with other co-purifying lower-molecular-weight CNS proteins, including alpha and beta tubulin. NFPs were partially purified by modi...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Brown BA,Nixon RA,Strocchi P,Marotta CA

    更新日期:1981-01-01 00:00:00

  • Expression of the choline acetyltransferase gene depends on protein kinase A activity.

    abstract::Choline acetyltransferase activity is barely detectable in a mutant pheochromocytoma PC12 cell line, A123.7, which is deficient in protein kinase A activity. Northern blot and polymerase chain reaction analyses showed that this mutant cell line has dramatically reduced levels of choline acetyltransferase mRNA, which c...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Inoue H,Li YP,Wagner JA,Hersh LB

    更新日期:1995-03-01 00:00:00

  • Potentiation by the tetraphenylboron anion of the effects of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and its pyridinium metabolite.

    abstract::The 1-methyl-4-phenylpyridinium species (MPP+) is the four-electron oxidation product of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and is widely assumed to be the actual neurotoxic species responsible for the MPTP-induced destruction of dopaminergic neurons. MPTP is oxidized by the enzyme monoamine oxidase-B...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Heikkila RE,Hwang J,Ofori S,Geller HM,Nicklas WJ

    更新日期:1990-03-01 00:00:00

  • Bdnf gene is a downstream target of Nurr1 transcription factor in rat midbrain neurons in vitro.

    abstract::The transcription factor Nurr1 is essential for the generation of midbrain dopaminergic neurons (mDA). Only a few Nurr1-regulated genes have so far been identified and it remains unclear how Nurr1 influences the development and function of dopaminergic neurons. To identify novel Nurr1 target genes we have used genome-...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Volpicelli F,Caiazzo M,Greco D,Consales C,Leone L,Perrone-Capano C,Colucci D'Amato L,di Porzio U

    更新日期:2007-07-01 00:00:00

  • Dynamic aspects of glucocorticoid receptors in the spinal cord of the rat.

    abstract::In spite of biochemical and autoradiographic evidence for glucocorticoid binding sites in the spinal cord (SC), events occurring after the preliminary step of hormone binding were not studied. In this investigation, we have examined the transformation (activation) of the cytosolic receptor coupled to [3H]dexamethasone...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Ortí E,Tornello S,De Nicola AF

    更新日期:1985-12-01 00:00:00

  • Enhanced behavioral sensitivity to the competitive GABA agonist, gaboxadol, in transgenic mice over-expressing hippocampal extrasynaptic alpha6beta GABA(A) receptors.

    abstract::The behavioral and functional significance of the extrasynaptic inhibitory GABA(A) receptors in the brain is still poorly known. We used a transgenic mouse line expressing the GABA(A) receptor alpha6 subunit gene in the forebrain under the Thy-1.2 promoter (Thy1alpha6) mice ectopically expressing alpha6 subunits espec...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Saarelainen KS,Ranna M,Rabe H,Sinkkonen ST,Möykkynen T,Uusi-Oukari M,Linden AM,Lüddens H,Korpi ER

    更新日期:2008-04-01 00:00:00

  • Intra- and interchain disulfide bond generation in S100b protein.

    abstract::Disulfide-bridged S100b protein formation, aircatalyzed and induced by thiol/disulfide exchange, was studied under various ionic conditions. As native, physiological disulfide-bridged proteins are obtained easily from their reduced counterparts under appropriate redox conditions, this work was performed to determine w...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Mely Y,Gérard D

    更新日期:1990-10-01 00:00:00

  • RCAN1 regulates vesicle recycling and quantal release kinetics via effects on calcineurin activity.

    abstract::We have previously shown that Regulator of Calcineurin 1 (RCAN1) regulates multiple stages of vesicle exocytosis. However, the mechanisms by which RCAN1 affects secretory vesicle exocytosis and quantal release kinetics remain unknown. Here, we use carbon fibre amperometry to detect exocytosis from chromaffin cells and...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Zanin MP,Mackenzie KD,Peiris H,Pritchard MA,Keating DJ

    更新日期:2013-02-01 00:00:00

  • Histamine stimulation of cyclic AMP accumulation in astrocyte-enriched and neuronal primary cultures from rat brain.

    abstract::Histamine stimulates cyclic AMP accumulation in astrocyte-enriched and neuronal primary cultures from rat brain in the presence of the phosphodiesterase inhibitor isobutylmethylxanthine. The response in the astrocyte cultures (Emax = 304 +/- 44% over basal, EC50 = 43 +/- 5 microM) was much higher than in neuronal cult...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Agulló L,Picatoste F,García A

    更新日期:1990-11-01 00:00:00

  • Regulation of myelin-associated glycoprotein binding by sialylated cis-ligands.

    abstract::Myelin-associated glycoprotein (MAG) and Schwann cell myelin protein (SMP) are highly glycosylated members of a newly defined family of cell adhesion molecules belonging to the immunoglobulin superfamily that recognize terminal sialic acid residues on N- and O-linked oligosaccharides. The importance of the N-linked ol...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Tropak MB,Roder JC

    更新日期:1997-04-01 00:00:00

  • Dopamine D2 receptor signaling modulates mutant ataxin-1 S776 phosphorylation and aggregation.

    abstract::Spinocerebellar ataxia 1 (SCA1) is a dominantly inherited neurodegenerative disease associated with progressive ataxia resulting from the loss of cerebellar Purkinje cells (PCs) and neurons in the brainstem. In PCs of SCA1 transgenic mice, the disease causing ataxin-1 protein mediates the formation of S100B containing...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Hearst SM,Lopez ME,Shao Q,Liu Y,Vig PJ

    更新日期:2010-08-01 00:00:00

  • Delayed genomic responses to transient middle cerebral artery occlusion in the rat.

    abstract::Ischemic stress in the brain induces acute and massive neuronal death in the targeted area, which is followed by a second round of detrimental processes, called delayed neuronal death, in the neighboring areas. To test the hypothesis that transcriptional control plays a role in the pathophysiology of the postischemic ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Kim JB,Piao CS,Lee KW,Han PL,Ahn JI,Lee YS,Lee JK

    更新日期:2004-06-01 00:00:00

  • The effect of Cyclin-dependent kinase 5 on voltage-dependent calcium channels in PC12 cells varies according to channel type and cell differentiation state.

    abstract::Cyclin-dependent kinase 5 (Cdk5) is a Ser/Thr kinase that plays an important role in the release of neurotransmitter from pre-synaptic terminals triggered by Ca(2+) influx into the pre-synaptic cytoplasm through voltage-dependent Ca(2+) channels (VDCCs). It is reported that Cdk5 regulates L-, P/Q-, or N-type VDCC, but...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Furusawa K,Asada A,Saito T,Hisanaga S

    更新日期:2014-08-01 00:00:00

  • Mitochondrial free radical signal in ceramide-dependent apoptosis: a putative mechanism for neuronal death in Parkinson's disease.

    abstract::Activation of the apoptogenic sphingomyelin-dependent signaling pathway in neuronally differentiated PC12 cells with cell-permeant C2-ceramide resulted in a transient and short-lived emission of reactive oxygen species that was maximal 6 h after the beginning of treatment, followed immediately by nuclear translocation...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: France-Lanord V,Brugg B,Michel PP,Agid Y,Ruberg M

    更新日期:1997-10-01 00:00:00

  • Effects of free fatty acids on synaptosomal amino acid uptake systems.

    abstract::The Na+-dependent synaptosomal uptakes of proline, aspartic acid, glutamic acid, and gamma-aminobutyric acid were strong inhibited by monounsaturated fatty acids. With oleic acid, half-maximal inhibition was observed at about 15 microM. The Na+-independent uptakes of leucine, phenylalanine, histidine, and valine were ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Rhoads DE,Kaplan MA,Peterson NA,Raghupathy E

    更新日期:1982-05-01 00:00:00

  • Pituitary adenylate cyclase-activating polypeptide (PACAP) stimulates the expression and the release of tissue plasminogen activator (tPA) in neuronal cells: involvement of tPA in the neuroprotective effect of PACAP.

    abstract::Pituitary adenylate cyclase-activating polypeptide (PACAP) and tissue plasminogen activator (tPA) play important roles in neuronal migration and survival. However, a direct link between the neurotrophic effects of PACAP and tPA has never been investigated. In this study, we show that, in PC12 cells, PACAP induced a 9....

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Raoult E,Roussel BD,Bénard M,Lefebvre T,Ravni A,Ali C,Vivien D,Komuro H,Fournier A,Vaudry H,Vaudry D,Galas L

    更新日期:2011-12-01 00:00:00

  • Neurochemical properties of enkephalinergic neurons in lumbar spinal dorsal horn revealed by preproenkephalin-green fluorescent protein transgenic mice.

    abstract::Enkephalin (ENK) has been implicated in nociceptive transmission in the spinal cord while its functional role is not clear because of difficulties in ideally visualizing ENKergic neurons. We thus developed preproenkephalin-green fluorescent protein transgenic mice to better identify ENKergic neurons. Real-time reverse...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Huang J,Chen J,Wang W,Wang W,Koshimizu Y,Wei YY,Kaneko T,Li YQ,Wu SX

    更新日期:2010-06-01 00:00:00

  • Acetyl-CoA and acetylcholine metabolism in nerve terminal compartment of thiamine deficient rat brain.

    abstract::The decrease of pyruvate and ketoglutarate dehydrogenase complex activities is the main cause of energy and acetyl-CoA deficits in thiamine deficiency-evoked cholinergic encephalopathies. However, disturbances in pathways of acetyl-CoA metabolism leading to appearance of cholinergic deficits remain unknown. Therefore,...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Jankowska-Kulawy A,Bielarczyk H,Pawełczyk T,Wróblewska M,Szutowicz A

    更新日期:2010-10-01 00:00:00

  • Chronic electroconvulsive seizures down-regulate expression of the immediate-early genes c-fos and c-jun in rat cerebral cortex.

    abstract::Acute seizures and other stimuli that increase neuronal activity cause a rapid induction of the immediate-early genes c-fos and c-jun, also referred to as nuclear proto-oncogenes, in the nervous system. In the present study, rats were administered one or more electroconvulsive seizures (ECS) and the responsiveness of ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Winston SM,Hayward MD,Nestler EJ,Duman RS

    更新日期:1990-06-01 00:00:00

  • Microtubule dynamics and the neurodegenerative triad of Alzheimer's disease: The hidden connection.

    abstract::Alzheimer's disease (AD) is the most common neurodegenerative disorder and is, on a histopathological level, characterized by the presence of extracellular amyloid plaques composed of the protein fragment Aβ, and intracellular neurofibrillary tangles, which contain the microtubule-associated protein tau in a hyperphos...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Brandt R,Bakota L

    更新日期:2017-11-01 00:00:00

  • Synaptotagmin I is a molecular target for lead.

    abstract::Lead poisoning can cause a wide range of symptoms with particularly severe clinical effects on the CNS. Lead can increase spontaneous neurotransmitter release but decrease evoked neurotransmitter release. These effects may be caused by an interaction of lead with specific molecular targets involved in neurotransmitter...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Bouton CM,Frelin LP,Forde CE,Arnold Godwin H,Pevsner J

    更新日期:2001-03-01 00:00:00

  • Characterization of [3H]quipazine binding to 5-hydroxytryptamine3 receptors in rat brain membranes.

    abstract::[3H]Quipazine was used to label binding sites in rat brain membranes that display characteristics of a 5-hydroxytryptamine3 (5-HT3) receptor. The radioligand binds with high affinity (KD, 1.2 +/- 0.1 nM) to a saturable population of sites (Bmax, 3.0 +/- 0.4 pmol/g of tissue) that are differentially located in the brai...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Milburn CM,Peroutka SJ

    更新日期:1989-06-01 00:00:00

  • MEK and ERK protect hypoxic cortical neurons via phosphorylation of Bad.

    abstract::We investigated the role of mitogen-activated protein kinase (MAPK) pathways in hypoxic neuronal injury using primary cultures from murine cerebral cortex. Hypoxia caused the death of approximately 50% of neurons at 16 h and approximately 65% of neurons at 24 h. This was associated with phospho-activation of the MAPK/...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Jin K,Mao XO,Zhu Y,Greenberg DA

    更新日期:2002-01-01 00:00:00

  • Local stimulation of the adenosine A2B receptors induces an increased release of IL-6 in mouse striatum: an in vivo microdialysis study.

    abstract::Both adenosine and interleukin-6 (IL-6) have been implicated in the pathophysiology of, e.g., epileptic seizures, traumatic brain injury, and affective disorders. Stimulation of adenosine A2B receptors on astrocytes in vitro leads to the increased synthesis and secretion of IL-6. We investigated whether or not activat...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Vazquez JF,Clement HW,Sommer O,Schulz E,van Calker D

    更新日期:2008-05-01 00:00:00

  • Shift in the ratio of three-repeat tau and four-repeat tau mRNAs in individual cholinergic basal forebrain neurons in mild cognitive impairment and Alzheimer's disease.

    abstract::Molecular mechanisms underlying tauopathy remain undetermined. In the current study, single cell gene expression profiling was coupled with custom-designed cDNA array analysis to evaluate tau expression and other cytoskeletal elements within individual neuronal populations in patients with no cognitive impairment (NCI...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Ginsberg SD,Che S,Counts SE,Mufson EJ

    更新日期:2006-03-01 00:00:00

  • Nicotine trapping causes the persistent desensitization of alpha4beta2 nicotinic receptors expressed in oocytes.

    abstract::To determine whether prolonged nicotine exposure persistently inactivates rat alpha4beta2 nicotinic receptors expressed in Xenopus oocytes, we measured the voltage-clamped alpha4beta2 response to acetylcholine (ACh) before and 24 h after, 1-h or 12-h incubations in 10 microm nicotine. A 12-h incubation in 10 microm ni...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Jia L,Flotildes K,Li M,Cohen BN

    更新日期:2003-02-01 00:00:00