Abstract：
OBJECTIVE:To determine the cell content and purity of Ficoll-separated peripheral blood mononuclear cells and granulocyte isolates in sepsis patients compared to healthy controls. DESIGN AND SETTING:Prospective study in the adult and pediatric intensive care departments of the Erasmus University Medical Center in the Netherlands. PATIENTS:Three sepsis patients (two adults, one child) and four healthy controls. MEASUREMENTS AND RESULTS:Blood leukocytes were separated by Ficoll into an interface and a bottom fraction. The cell content and purity was analyzed by cytospin and flow-cytometric immunofluorescence. In sepsis patients, the interface consisted of 11-52% mononuclear cells only, due to high contamination with granulocytes (48-89%). This was in contrast to a high proportion of mononuclear cells (88-100%) in healthy controls. The bottom fraction showed a cell purity of >or=92% polymorphonuclear granulocytes in sepsis patients as well as in healthy controls. CONCLUSIONS:Ficoll-separated leukocytes of sepsis patients are not suitable for studying mononuclear cells but can be used for studying granulocytes with high purity. The mononuclear cell fraction is highly contaminated with granulocytes. Additional separation techniques are necessary to obtain a pure cell fraction.
journal_name
Intensive Care Medjournal_title
Intensive care medicineauthors
van den Akker EL,Baan CC,van den Berg B,Russcher H,Joosten K,Hokken-Koelega AC,Lamberts SW,Koper JWdoi
10.1007/s00134-007-0989-0subject
Has Abstractpub_date
2008-05-01 00:00:00pages
912-6issue
5eissn
0342-4642issn
1432-1238journal_volume
34pub_type
杂志文章abstract：: ...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-018-5213-x
更新日期：2019-01-01 00:00:00
abstract：OBJECTIVE:To evaluate the incidence and the circumstances of a moderate increase in serum creatinine early after elective abdominal aortic surgery. DESIGN:Prospective clinical observational study. SETTING:Surgical intensive care unit in a university hospital. PATIENTS:Two hundred and fifteen consecutive adult patien...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-003-1958-x
更新日期：2003-10-01 00:00:00
abstract：:The reliability of cardiac output estimation by thermodilution during artificial ventilation was studied in anesthetized pigs at the right side of the heart. The estimates exhibited a cyclic modulation related to the ventilation. The amplitude of the modulation was independent of the level of positive end-expiratory p...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF00254515
更新日期：1986-01-01 00:00:00
abstract：:Several different epidemiological typing methods have been used in studies of coagulase-negative staphylococci; these include biotyping, antibiotic susceptibility pattern analysis, serological typing, phage typing, slime production detection, protein profile analysis, immunoblot fingerprinting and DNA typing. The most...
journal_title：Intensive care medicine
pub_type： 杂志文章,评审
doi：10.1007/BF01694703
更新日期：1993-01-01 00:00:00
abstract：:This narrative review focuses on thoracic ultrasonography (lung and pleural) with the aim of outlining its utility for the critical care clinician. The article summarizes the applications of thoracic ultrasonography for the evaluation and management of pneumothorax, pleural effusion, acute dyspnea, pulmonary edema, pu...
journal_title：Intensive care medicine
pub_type： 杂志文章,评审
doi：10.1007/s00134-019-05725-8
更新日期：2019-09-01 00:00:00
abstract：OBJECTIVE:To derive recommendations for the dosing of commonly used medications in the morbidly obese patient in the ICU. DATA SOURCES:Articles were obtained through computerized searches involving MEDLINE. The bibliographies of retrieved publications and textbooks were reviewed for additional references. STUDY SELEC...
journal_title：Intensive care medicine
pub_type： 杂志文章,评审
doi：10.1007/s00134-003-2059-6
更新日期：2004-01-01 00:00:00
abstract：:We report the intensive care management of 23 children (age 3-15 years) following orthotopic heart (HT) and combined heart and lung transplantation (HLT) performed at our 2 institutes between February 1985 and August 1989. Cyclosporin A, azathioprine and steroids were given as routine immunosuppression, whilst anti-th...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF01711219
更新日期：1990-01-01 00:00:00
abstract：OBJECTIVE:To examine the usefulness of preload indices obtained by transoesophageal echocardiography (TOE) for estimating stroke volume at various levels of cardiac index. DESIGN:Prospective clinical study. SETTING:Intensive care unit with surgical patients. PATIENTS:16 ventilated patients monitored via Swan-Ganz ca...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s001340050349
更新日期：1997-04-01 00:00:00
abstract：OBJECTIVES:To establish a preliminary list of critical incidents (CIs) associated with mechanical ventilation and to describe a CI reporting method. DESIGN:A list of CIs was established based on a consensus among ICU caregivers. The list was compared to CIs collected prospectively during a predefined study period. SE...
journal_title：Intensive care medicine
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/s00134-002-1251-4
更新日期：2002-04-01 00:00:00
abstract：PURPOSE:Neutrophil gelatinase-associated lipocalin (NGAL) is a promising biomarker for acute kidney injury (AKI). We evaluated the diagnostic and prognostic accuracies of plasma NGAL (pNGAL) for contrast-induced AKI (CI-AKI) in critically ill patients. METHODS:In a prospective observational study in two adult intensiv...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-013-2826-y
更新日期：2013-05-01 00:00:00
abstract：PURPOSE:Acute kidney injury (AKI) frequently occurs in critically ill patients and often precipitates use of renal replacement therapy (RRT). However, the ideal circumstances for whether and when to start RRT remain unclear. We performed evidence synthesis of the available literature to evaluate the value of biomarkers...
journal_title：Intensive care medicine
pub_type： 杂志文章,评审
doi：10.1007/s00134-018-5126-8
更新日期：2018-03-01 00:00:00
abstract：OBJECTIVE:Measurement of functional residual capacity (FRC) during mechanical ventilation is important to standardise respiratory system compliance and adjust the ventilator settings to optimise lung recruitment. In the present study we compared three methods to measure FRC. DESIGN:The bias flow nitrogen washout techn...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-003-2028-0
更新日期：2004-01-01 00:00:00
abstract：OBJECTIVE:The liver plays a key role in the inflammatory response during major surgery or infection. The influence of age on liver function and hepatic acute phase protein (APP) synthesis should be serially studied in elderly patients undergoing major abdominal surgery. DESIGN AND SETTING:Prospective and descriptive s...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-001-1126-0
更新日期：2001-11-01 00:00:00
abstract：PURPOSE:Information on predisposing risk factors influencing long-term survival after extracorporeal membrane oxygenation (ECMO) support remains scarce. In critically ill patients chronic obstructive pulmonary disease (COPD) is an independent risk factor for mortality and morbidity. We assessed the influence of COPD on...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-013-2931-y
更新日期：2013-08-01 00:00:00
abstract：OBJECTIVE:To investigate the accuracy of lung ultrasonography (LUS) in the quantification of lung water in critically ill patients by using quantitative computed tomography (CT) as the gold standard for the determination of lung weight. METHODS:Twenty consecutive patients admitted to an intensive care unit who underwe...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-012-2694-x
更新日期：2013-01-01 00:00:00
abstract：PURPOSE:Severe acute pancreatitis (SAP) remains a life-threatening disease with classic etiology of systemic inflammatory response and mortality between 30 and 50 %. The aim of the present study is to compare two different treatment strategies of goal-directed hemodynamic management and evaluate their impact on surviva...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-012-2775-x
更新日期：2013-04-01 00:00:00
abstract：: ...
journal_title：Intensive care medicine
pub_type： 杂志文章,评审
doi：10.1007/s00134-018-5107-y
更新日期：2018-11-01 00:00:00
abstract：:Recent advances in analytical electrochemistry have provided highly selective potassium ion exchangers and robust polymeric membranes that can be used to measure the potassium activity in plasma or whole blood. The relationship between the activity measurement and the more familiar concentration measurement is explore...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF01684390
更新日期：1978-04-01 00:00:00
abstract：:A case of brainstem encephalitis complicated by torsade de pointes is described. The possible occurrence of ventricular arrhythmias may contribute to the mortality in this condition. We recommend the admission of patients with brainstem encephalitis to an intensive care unit, for a period of electrocardiographic monit...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF00263564
更新日期：1987-01-01 00:00:00
abstract：OBJECTIVE:To present a concise history of tracheostomy and tracheal intubation for the approximately forty centuries from their earliest description around 2000 BC until the middle of the twentieth century, at which time a proliferation of advances marked the beginning of the modern era of anesthesiology. DATA SOURCES...
journal_title：Intensive care medicine
pub_type： 历史文章,杂志文章
doi：10.1007/s00134-007-0931-5
更新日期：2008-02-01 00:00:00
abstract：OBJECTIVE:To determine the frequency and the eventual clinical characteristics of nosocomial sinusitis with anaerobic bacteria isolation in patients in the intensive care unit (ICU). DESIGN:Retrospective study. SETTING:A 12-bed medical ICU in a teaching hospital. PATIENTS:30 adult patients with documented nosocomial...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s001340051013
更新日期：1999-10-01 00:00:00
abstract：PURPOSE:We conducted a retrospective cohort study in an academic tertiary care center to characterize ventilator-associated pneumonia (VAP) in pediatric patients after cardiac surgery in The Netherlands. METHODS:All patients following cardiac surgery and mechanically ventilated for ≥24 h were included. The primary out...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-011-2349-3
更新日期：2011-10-01 00:00:00
abstract：OBJECTIVE:Infection is the most frequent cause of acute respiratory distress syndrome (ARDS). However, little is known about the influence of infection sites on ARDS. This study aimed to assess the associations of infection sites with ARDS development and mortality in critically ill infected patients. DESIGN:Prospecti...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-010-1851-3
更新日期：2010-06-01 00:00:00
abstract：PURPOSE:Extracorporeal membrane oxygenation (ECMO) can support oxygenation and carbon dioxide elimination in severe lung failure. Usually it is accompanied by controlled mechanical ventilation. Neurally adjusted ventilatory assist (NAVA) is a new mode of ventilation triggered by the diaphragmatic electrical activity an...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-010-1982-6
更新日期：2010-12-01 00:00:00
abstract：OBJECTIVE:To determine the incidence rates of hospital acquired infections (HAI) during the first 14 days after ICU discharge after treatment during ICU-stay with Selective Decontamination of the Digestive tract (SDD), Selective Oropharyngeal Decontamination (SOD) or Standard Care (SC). DESIGN:Prospective observationa...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-009-1554-9
更新日期：2009-09-01 00:00:00
abstract：OBJECTIVE:To determine the value of procalcitonin (PCT) in the early diagnosis (and differentiation) of patients with SIRS, sepsis, severe sepsis, and septic shock in comparison to C-reactive protein (CRP), white blood cell and thrombocyte count, and APACHE-II score (AP-II). DESIGN:Prospective cohort study including a...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF02900728
更新日期：2000-03-01 00:00:00
abstract：INTRODUCTION:The purpose of a scientific paper is to communicate results and within the paper this applies especially to the presentation of data. It is the universal practice in medical journals to present statistical results using tables. Good tables are an integral part of the manuscript. To help researchers communi...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-014-3248-1
更新日期：2014-05-01 00:00:00
abstract：OBJECTIVES:To establish priorities for research in critical care medicine in the UK using survey and nominal group (NG) techniques. DESIGN:The senior doctor and nurse from 325 intensive care units (ICUs) in the UK were invited to contribute up to ten research questions relevant to intensive care organisation, practice...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s001340000628
更新日期：2000-10-01 00:00:00
abstract：:We report the case of a 72-year-old male who suffered a cardiac arrest during an early positive treadmill stress test. After successful resuscitation the patient had evidence of a gastric perforation. Because of his hemodynamic stability, lack of peritoneal signs, and prohibitively high surgical risk, a non-operative ...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF01710656
更新日期：1994-07-01 00:00:00
abstract：OBJECTIVE:To describe the incidence, risk factors, and impact on mortality of acute kidney injury (AKI) in patients with 2009 influenza A (H1N1) viral pneumonia requiring mechanical ventilation. DESIGN:Observational cohort study. PATIENTS AND METHODS:AKI was defined as risk, injury or failure, according to the RIFLE ...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-011-2167-7
更新日期：2011-05-01 00:00:00
