Abstract：
OBJECTIVE:To determine the cell content and purity of Ficoll-separated peripheral blood mononuclear cells and granulocyte isolates in sepsis patients compared to healthy controls. DESIGN AND SETTING:Prospective study in the adult and pediatric intensive care departments of the Erasmus University Medical Center in the Netherlands. PATIENTS:Three sepsis patients (two adults, one child) and four healthy controls. MEASUREMENTS AND RESULTS:Blood leukocytes were separated by Ficoll into an interface and a bottom fraction. The cell content and purity was analyzed by cytospin and flow-cytometric immunofluorescence. In sepsis patients, the interface consisted of 11-52% mononuclear cells only, due to high contamination with granulocytes (48-89%). This was in contrast to a high proportion of mononuclear cells (88-100%) in healthy controls. The bottom fraction showed a cell purity of >or=92% polymorphonuclear granulocytes in sepsis patients as well as in healthy controls. CONCLUSIONS:Ficoll-separated leukocytes of sepsis patients are not suitable for studying mononuclear cells but can be used for studying granulocytes with high purity. The mononuclear cell fraction is highly contaminated with granulocytes. Additional separation techniques are necessary to obtain a pure cell fraction.
journal_name
Intensive Care Medjournal_title
Intensive care medicineauthors
van den Akker EL,Baan CC,van den Berg B,Russcher H,Joosten K,Hokken-Koelega AC,Lamberts SW,Koper JWdoi
10.1007/s00134-007-0989-0subject
Has Abstractpub_date
2008-05-01 00:00:00pages
912-6issue
5eissn
0342-4642issn
1432-1238journal_volume
34pub_type
杂志文章abstract：:The original version of this article unfortunately contained a mistake. ...
journal_title：Intensive care medicine
pub_type： 杂志文章,已发布勘误
doi：10.1007/s00134-020-06009-2
更新日期：2020-05-01 00:00:00
abstract：OBJECTIVE:Erythrocytes influence the magnitude of hypoxia-induced pulmonary artery pressure increase. It is, however, unknown to what extent haemoconcentration and haemodilution affect this response and whether intrapulmonary blood volume (and thus vessel dimensions) alters the magnitude of pressure increase. Furthermo...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s001340051327
更新日期：2000-08-01 00:00:00
abstract：PURPOSE:Hypoxic hepatitis (HH) is a form of hepatic injury following arterial hypoxemia, ischemia, and passive congestion of the liver. We investigated the incidence and the prognostic implications of HH in the medical intensive care unit (ICU). METHODS:A total of 1,066 consecutive ICU admissions at three medical ICUs...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-011-2248-7
更新日期：2011-08-01 00:00:00
abstract：OBJECTIVE:To evaluate the extent to which respiratory changes in inferior vena cava (IVC) diameter can be used to predict fluid responsiveness. DESIGN:Prospective clinical study. SETTING:Hospital intensive care unit. PATIENTS:Twenty-three patients with acute circulatory failure related to sepsis and mechanically ven...
journal_title：Intensive care medicine
pub_type： 临床试验,杂志文章
doi：10.1007/s00134-004-2259-8
更新日期：2004-09-01 00:00:00
abstract：OBJECTIVES:(a). To describe the glucose content of normal human airways secretions; (b). to observe the effects of hyperglycemia and airways inflammation on airways glucose. DESIGN:Observational studies. SETTINGS:(a). St George's Hospital Medical School; (b). diabetes mellitus outpatient clinics; (c). adult general i...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-003-1961-2
更新日期：2003-12-01 00:00:00
abstract：OBJECTIVE:To investigate the sources of hemorrhage and use of endoscopic hemostasis in patients with clinically significant upper gastrointestinal (UGI) hemorrhage after admission to the intensive care unit (ICU). DESIGN AND SETTING:Prospective study, 123 beds of ICU in a 1,629-bed medical center. MEASUREMENTS AND RE...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-003-1921-x
更新日期：2003-10-01 00:00:00
abstract：OBJECTIVE:To test the hypothesis that the rate of degradation of exogenously administered albumin is faster with bolus administration than with continuous infusion and thus that a bolus administration is less efficacious in restoring blood albumin concentration (BAC) in the hypoalbuminemic critically ill pediatric pati...
journal_title：Intensive care medicine
pub_type： 临床试验,杂志文章
doi：10.1007/BF01712175
更新日期：1996-05-01 00:00:00
abstract：OBJECTIVE:To determine the presence of tricuspid regurgitation (TR) in patients affected by acute lung injury (ALI) and the adult respiratory distress syndrome (ARDS) during mechanical ventilation with positive end-expiratory pressure (PEEP). DESIGN:A prospective clinical study. SETTING:10-bed general intensive care ...
journal_title：Intensive care medicine
pub_type： 临床试验,杂志文章
doi：10.1007/s001340050419
更新日期：1997-08-01 00:00:00
abstract：OBJECTIVE:To investigate the hemodynamic and metabolic effects of the peroxisome proliferator-activated receptor (PPAR)-gamma ligand and nuclear-factor (NF)-kappa B inhibitor 15-deoxy-Delta12,14-prostaglandin-J2 (15d-PGJ2) during long-term, hyperdynamic porcine endotoxemia. DESIGN:Prospective, randomized, controlled e...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-006-0107-8
更新日期：2006-05-01 00:00:00
abstract：OBJECTIVE:This study compared the recently introduced Microcuff endotracheal tube HVLP ICU featuring an ultrathin (7-microm) polyurethane cuff membrane with endotracheal tubes from different manufacturers regarding fluid leakage past the tube cuff. DESIGN:In vitro setup. MEASUREMENTS AND RESULTS:The following endotra...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-003-1933-6
更新日期：2003-10-01 00:00:00
abstract：PURPOSE:We compared 1 versus 0.5 microg/kg bolus remifentanil versus placebo in alleviating pain due to chest drain removal. Effects on sedation, respiratory rate (RR), oxygen saturation, heart rate (HR) and blood pressure were also evaluated. METHODS:Sixty patients following cardiac surgery were enrolled in this pros...
journal_title：Intensive care medicine
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00134-010-1836-2
更新日期：2010-08-01 00:00:00
abstract：OBJECTIVE:To assess if the observed respiratory cycle-related variation in intra-abdominal pressure is reliably quantifiable and a possible indirect indicator of abdominal compliance. Secondary issues were to assess the roles played by respiratory parameters in determining this oscillation and by patients' position in ...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-008-1155-z
更新日期：2008-09-01 00:00:00
abstract：OBJECTIVE:To evaluate trends in mortality and related factors among trauma patients who developed acute respiratory distress syndrome (ARDS). STUDY:Observational study based on data prospectively gathered in computerized trauma registry. SETTING:Trauma intensive care unit (ICU) of 48 beds in level I trauma center. P...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s001340100955
更新日期：2001-07-01 00:00:00
abstract：OBJECTIVE:Five commercially available oesophageal balloon catheters (OBCs) were tested to evaluate the accuracy in transmitting fast-changing pressure signals which can be observed, for example, during phrenic nerve stimulation. SETTING:Research laboratory of a university hospital. METHOD:The OBCs tested varied in le...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s001340051182
更新日期：2000-04-01 00:00:00
abstract：:Several different epidemiological typing methods have been used in studies of coagulase-negative staphylococci; these include biotyping, antibiotic susceptibility pattern analysis, serological typing, phage typing, slime production detection, protein profile analysis, immunoblot fingerprinting and DNA typing. The most...
journal_title：Intensive care medicine
pub_type： 杂志文章,评审
doi：10.1007/BF01694703
更新日期：1993-01-01 00:00:00
abstract：:The process of surgical decision making is based on both general principles that are amenable to evaluation using rigorous techniques of clinical research and the intangible element of surgical judgment that seeks to apply those principles to the care of an individual patient. The role of surgical judgment is inescapa...
journal_title：Intensive care medicine
pub_type： 指南,杂志文章,实务指引,评审
doi：10.1007/pl00003797
更新日期：2001-01-01 00:00:00
abstract：OBJECTIVE:Aspiration of dead space (ASPIDS) improves carbon dioxide (CO2) elimination by replacing dead space air rich in CO2 with fresh gas during expiration. The hypothesis was that ASPIDS allows normocapnia to be maintained at low tidal volumes (VT). DESIGN:Prospective study. SETTING:Adult intensive care unit in a...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s001340050929
更新日期：1999-07-01 00:00:00
abstract：OBJECTIVE:To compare the total plasma cortisol values obtained from three widely used immunoassays and a high pressure liquid chromatography (HPLC) technique on samples obtained from patients with sepsis. DESIGN AND SETTING:Observational interventional in the general intensive care unit of a metropolitan hospital PAT...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-006-0389-x
更新日期：2006-11-01 00:00:00
abstract：BACKGROUND:Posterior reversible encephalopathy syndrome (PRES) is a well-recognized clinico-neuroradiological transient condition. Early recognition is of paramount importance for prompt control of blood pressure or removal of precipitating factors and treatment of epileptic seizures or status epilepticus. Delay in the...
journal_title：Intensive care medicine
pub_type： 杂志文章,评审
doi：10.1007/s00134-006-0459-0
更新日期：2007-02-01 00:00:00
abstract：:The recording of intratracheal pressure in patients breathing through a tracheostomy tube is marred by methodological problems. In model experiments it has been shown that the introduction of a recording catheter into the tracheostomy tube alters the recorded pressure by as much as 20-40%. To avoid such inaccuracy, a ...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF01735176
更新日期：1990-01-01 00:00:00
abstract：OBJECTIVES:To examine whether urgent orotracheal intubation (OI) can induce bacteremia. To find predictive factors for post-intubation bacteremia. DESIGN:Prospective observational study. SETTING:Seventeen-bed medical intensive care unit in a university hospital. PATIENTS:Sixty-eight adult intensive care patients und...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s001340000841
更新日期：2001-02-01 00:00:00
abstract：OBJECTIVE:To evaluate the efficacy of different lung recruitment maneuvers using electric impedance tomography. DESIGN AND SETTING:Experimental study in animal model of acute lung injury in an animal research laboratory. SUBJECTS:Fourteen pigs with saline lavage induced lung injury. INTERVENTIONS:Lung volume, region...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-005-2799-6
更新日期：2005-12-01 00:00:00
abstract：:A veno-venous to-and-fro bypass method through a single blood access for extracorporeal lung assist with an artificial membrane lung is introduced. A premature newborn with severe respiratory distress was treated with this method. A 12 Fr. single lumen catheter with a spiral-embedded thin-wall, 0.25 mm in wall thickne...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF00271065
更新日期：1989-01-01 00:00:00
abstract：:Paediatric admissions to a general ITU are briefly reviewed. Although it may not be an ideal arrangement it is suggested that children can be safely cared for in such a unit. The problems created by the child with acute upper airway obstruction are discussed to demonstrate the way in which the unit is organized to rec...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF00260356
更新日期：1985-01-01 00:00:00
abstract：OBJECTIVE:The role of haemofiltration as an adjunctive treatment of sepsis remains a contentious issue. To address the role of dose and to explore the biological effects of haemofiltration we compared the effects of standard and high-volume haemofiltration (HVHF) in a peritonitis-induced model of porcine septic shock. ...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-008-1318-y
更新日期：2009-02-01 00:00:00
abstract：OBJECTIVE:Although several advantages are attributed to tracheotomy in ICU patients requiring mechanical ventilation (MV), true benefits and the optimal timing of tracheotomy remain controversial. In this study, we compared early tracheotomy (ET) with prolonged intubation (PI) in severely ill patients requiring prolong...
journal_title：Intensive care medicine
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1007/s00134-008-1195-4
更新日期：2008-10-01 00:00:00
abstract：OBJECTIVE:To test the hypothesis whether PMN-Elastase in bronchoalveolar lavage fluid (BALF) could reflect neutrophil activity in the lower respiratory tract. DESIGN:Prospectively, morphologic findings of acute and chronic inflammation in the transbronchial lung biopsy specimens were compared with the numbers of neutr...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF01720908
更新日期：1994-05-01 00:00:00
abstract：PURPOSE:Patients undergoing cardiac surgery often require transfusions of red blood cells, plasma and platelets. These components differ widely in both indications for use and composition. However, from a statistical point of view there is a significant colinearity between the components. This study explores the relati...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-012-2723-9
更新日期：2013-03-01 00:00:00
abstract：OBJECTIVE:To investigate the relationship between cytokines and the inflammatory responses in patients with out-of-hospital cardiac arrest, we examined the changes of cytokines as well as alterations in the markers of neutrophil activation, platelet and endothelial activation, and endothelial injury. DESIGN:Prospectiv...
journal_title：Intensive care medicine
pub_type： 临床试验,杂志文章
doi：10.1007/s001340050907
更新日期：1999-06-01 00:00:00
abstract：OBJECTIVE:To investigate the effectiveness of an enhanced software Model Predictive Control (eMPC) algorithm for intravenous insulin infusion, targeted at tight glucose control in critically ill patients, over 72 h, in two intensive care units with different management protocols. DESIGN:Comparison with standard care i...
journal_title：Intensive care medicine
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1007/s00134-008-1236-z
更新日期：2009-01-01 00:00:00
