Abstract：
OBJECTIVE:To determine the cell content and purity of Ficoll-separated peripheral blood mononuclear cells and granulocyte isolates in sepsis patients compared to healthy controls. DESIGN AND SETTING:Prospective study in the adult and pediatric intensive care departments of the Erasmus University Medical Center in the Netherlands. PATIENTS:Three sepsis patients (two adults, one child) and four healthy controls. MEASUREMENTS AND RESULTS:Blood leukocytes were separated by Ficoll into an interface and a bottom fraction. The cell content and purity was analyzed by cytospin and flow-cytometric immunofluorescence. In sepsis patients, the interface consisted of 11-52% mononuclear cells only, due to high contamination with granulocytes (48-89%). This was in contrast to a high proportion of mononuclear cells (88-100%) in healthy controls. The bottom fraction showed a cell purity of >or=92% polymorphonuclear granulocytes in sepsis patients as well as in healthy controls. CONCLUSIONS:Ficoll-separated leukocytes of sepsis patients are not suitable for studying mononuclear cells but can be used for studying granulocytes with high purity. The mononuclear cell fraction is highly contaminated with granulocytes. Additional separation techniques are necessary to obtain a pure cell fraction.
journal_name
Intensive Care Medjournal_title
Intensive care medicineauthors
van den Akker EL,Baan CC,van den Berg B,Russcher H,Joosten K,Hokken-Koelega AC,Lamberts SW,Koper JWdoi
10.1007/s00134-007-0989-0subject
Has Abstractpub_date
2008-05-01 00:00:00pages
912-6issue
5eissn
0342-4642issn
1432-1238journal_volume
34pub_type
杂志文章abstract：: ...
journal_title：Intensive care medicine
pub_type： 社论
doi：10.1007/s00134-018-5401-8
更新日期：2018-12-01 00:00:00
abstract：PURPOSE:In critically ill pediatric patients, fluid overload (FO) >10% has been identified as a threshold for possible interventions, including initiation of continuous renal replacement therapy (CRRT). However, multiple definitions have been reported, and there remains no consensus method for FO calculation. The goal ...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-012-2503-6
更新日期：2012-04-01 00:00:00
abstract：:In the past two years several guidelines for suggested components of emergency medical systems for children have been suggested, and for the first time, specific standards of pediatric trauma care have also been formulated. The American Academy of Pediatrics new Provisional Committee on Emergency Medicine has been cha...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF00260888
更新日期：1989-01-01 00:00:00
abstract：:Induced hypothermia can be used to protect the brain from post-ischemic and traumatic neurological injury. Potential clinical applications and the available evidence are discussed in a separate paper. This review focuses on the practical aspects of cooling and physiological changes induced by hypothermia, as well as t...
journal_title：Intensive care medicine
pub_type： 杂志文章,评审
doi：10.1007/s00134-003-2151-y
更新日期：2004-05-01 00:00:00
abstract：:Of 28 consecutive patients over 64 years old, in whom endoscopy revealed gastro-duodenal ulcers with signs of recent hemorrhage (active bleeding, non-bleeding vessel or adherent clot), 14 were randomly assigned to receive endoscopic alcoholization of the lesions preceded by intragastric instillation of norepinephrine,...
journal_title：Intensive care medicine
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/BF01724799
更新日期：1990-01-01 00:00:00
abstract：OBJECTIVE:To examine the attitudes of the general public regarding who should decide about the withdrawal of life support and to compare these attitudes with those of intensive care personnel. DESIGN:Nationwide postal questionnaire survey. SETTING:Sweden. PARTICIPANTS:One thousand one hundred ninety-six randomly sel...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s001340050987
更新日期：1999-09-01 00:00:00
abstract：OBJECTIVE:To evaluate the Datex Deltatrac II for measurements in neonates requiring mechanical ventilation. DESIGN:Prospective laboratory evaluation, using a ventilated lung model and gas injection. During simulation of 79 neonatal respiratory settings, assessment of oxygen consumption (VO2), carbon dioxide production...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s001340000708
更新日期：2001-01-01 00:00:00
abstract：OBJECTIVE:Evaluation of the distribution and antibiotic susceptibility of the aerobic gram-negative bacilli (AGNB) isolated from patients in intensive care units (ICU study). DESIGN AND SETTING:Microbiological study carried out in 1991 in 39 teaching hospitals. A standardized method was used to determine the minimum i...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF01699228
更新日期：1996-10-01 00:00:00
abstract：OBJECTIVE:The study was undertaken to determine if critically ill patients under mechanical ventilation could reactivate latent cytomegalovirus (CMV) in either lung or blood. DESIGN:Prospective study in critically ill patients was performed in a multidisciplinary intensive care unit in a university hospital. PATIENTS...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF01709343
更新日期：1996-11-01 00:00:00
abstract：PURPOSE:Because non-invasive mechanical ventilation (NIV) is increasingly used, new devices, both ventilators and interfaces, have been continuously proposed for clinical use in recent years. To provide the clinicians with valuable information about ventilators and interfaces for NIV, several bench studies evaluating a...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-011-2416-9
更新日期：2012-01-01 00:00:00
abstract：OBJECTIVE:To describe Enterococcus faecalis colonisation and endocarditis in 5 intensive care patients after treatment with selective decontamination (SDD). SETTING:Intensive care unit (ICU) in a general hospital. PATIENTS:The patients were admitted to the ICU because of adult respiratory distress syndrome, polytraum...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF01701478
更新日期：1995-03-01 00:00:00
abstract：OBJECTIVE:Percutaneous dilatational tracheostomy is increasingly practiced in intensive care units and has a low incidence of early complications. The late effects of this procedure are still poorly known and were the focus of this study. DESIGN:Prospective descriptive clinical study. SETTING:Interdisciplinary intens...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF01706200
更新日期：1995-06-01 00:00:00
abstract：:A young woman with long-standing systemic lupus erythematosus and rheumatic fever presented with deteriorating renal function. She had severe gastritis treated with cimetidine and received methylprednisolone when her blood cultures were repeatedly negative. She developed spontaneous bruising and bleeding from venepunc...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF01724803
更新日期：1990-01-01 00:00:00
abstract：PURPOSE:To examine the consequences of administration of norepinephrine on muscle tissue oxygenation in severely hypotensive septic shock patients. METHODS:This was a prospective observational study conducted in a medical intensive care unit of a university hospital. We included 28 septic shock patients that received ...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-010-2013-3
更新日期：2010-11-01 00:00:00
abstract：RATIONALE:Acute respiratory distress syndrome (ARDS) is frequently associated with hemodynamic instability which appears as the main factor associated with mortality. Shock is driven by pulmonary hypertension, deleterious effects of mechanical ventilation (MV) on right ventricular (RV) function, and associated-sepsis. ...
journal_title：Intensive care medicine
pub_type： 杂志文章,评审
doi：10.1007/s00134-016-4326-3
更新日期：2016-05-01 00:00:00
abstract：:Results of the relationships between oxygen supply, demand, and uptake can be used to interpret cardiac output values, identify types of acute circulatory failure, guide attempts to improve cellular function, and thus prevent the development of multiple organ failure and death. Five steps in the interpretation of card...
journal_title：Intensive care medicine
pub_type： 杂志文章,评审
doi：10.1007/BF01785244
更新日期：1990-01-01 00:00:00
abstract：OBJECTIVE:To investigate the effects of some acid-base abnormalities on blood capacity of transporting CO(2). DESIGN:Prospective study. SETTING:General and Cardiosurgical ICUs of a University hospital. PATIENTS:Six groups of ten patients characterized by: metabolic alkalosis; respiratory alkalosis; absence of acid-b...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-002-1259-9
更新日期：2002-05-01 00:00:00
abstract：PURPOSE:To evaluate the efficacy and safety of intravenous sildenafil for immediate postoperative pulmonary hypertension (PH) in pediatric patients undergoing congenital heart surgery. METHODS:A double-blind, multicenter, placebo-controlled, dose-ranging, parallel-group trial was conducted. Patients were randomized to...
journal_title：Intensive care medicine
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1007/s00134-010-2065-4
更新日期：2011-03-01 00:00:00
abstract：:A 42 year old pregnant woman was admitted in acute respiratory failure. Viral pneumonia was suspected and oxygen therapy, CPAP, water restriction and diuretics were started with good response. She remained febrile and had an abnormal chest X-ray, a diagnosis of miliary tuberculosis was confirmed by transbronchial fibr...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF01902549
更新日期：1978-11-01 00:00:00
abstract：OBJECTIVE:To determine the incidence of Clostridium difficile (CD) diarrhoea in feed-intolerant, critically ill patients who received erythromycin-based prokinetic therapy. DESIGN AND SETTING:Prospective observational study in a mixed intensive care unit. METHODS:The development of diarrhoea (> 3 loose, liquid stool ...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-007-0834-5
更新日期：2008-01-01 00:00:00
abstract：BACKGROUND:It remains uncertain why immunocompetent patients with bacterial community-acquired pneumonia (CAP) die, in spite of adequate antibiotics. METHODS:This is a secondary analysis of the CAPUCI database which was a prospective observational multicentre study. Two hundred and twelve immunocompetent patients admi...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-008-1363-6
更新日期：2009-03-01 00:00:00
abstract：OBJECTIVES:Critical illness increases the tendency to both coagulation and bleeding, complicating anticoagulation for continuous renal replacement therapy (CRRT). We analyzed strategies for anticoagulation in CRRT concerning implementation, efficacy and safety to provide evidence-based recommendations for clinical prac...
journal_title：Intensive care medicine
pub_type： 杂志文章,meta分析,评审
doi：10.1007/s00134-005-0044-y
更新日期：2006-02-01 00:00:00
abstract：OBJECTIVE:To compare available instruments for assessing delirium in critically ill adults that have undergone validity testing and provide clinicians with strategies to incorporate these instruments into clinical practice. DESIGN:Medline (1966-September 2006) was searched using the key words: delirium, cognitive dysf...
journal_title：Intensive care medicine
pub_type： 杂志文章,评审
doi：10.1007/s00134-007-0603-5
更新日期：2007-06-01 00:00:00
abstract：OBJECTIVE:To determine whether health-related quality of life (HRQL) in survivors of acute renal failure (ARF) occurring in intensive care is predictable from data available at the time of acute illness. DESIGN AND SETTING:Prospective pilot study in academic intensive care units. PATIENTS AND METHODS:Patients requiri...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-003-1837-5
更新日期：2003-09-01 00:00:00
abstract：OBJECTIVE:Capillary leakage syndrome (CLS) is a frequent complication in sepsis, characterized by loss of intravasal fluids leading to generalized edema and hemodynamic instability despite massive fluid therapy. In spite of its importance no standardized diagnostic criteria are available for CLS. DESIGN:Prospective cl...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s001340000601
更新日期：2000-09-01 00:00:00
abstract：OBJECTIVES:Previous studies on therapeutic interventions in sepsis have assumed stability of the measure of splanchnic blood flow throughout the study. We assessed the variability of splanchnic blood flow during stable global hemodynamics in eight patients with sepsis requiring treatment with dobutamine and/or norepine...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s001340100980
更新日期：2001-08-01 00:00:00
abstract：:Acetazolamide (Diamox) induced carbonic anhydrase inhibition is an efficient means of eliminating surplus water and bicarbonate in the overhydrated and alkalotic patient. Previous studies have demonstrated an unexpected and unexplained increase in arterial and venous oxygenation during acute carbonic anhydrase inhibit...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF00255787
更新日期：1987-01-01 00:00:00
abstract：OBJECTIVE:To evaluate clinical and microbiologic characteristics, modalities of treatment and outcome of patients with cervical necrotizing fasciitis admitted to our institution. DESIGN:Retrospective clinical investigation. PATIENTS AND METHODS:We reviewed the charts of 20 consecutive patients hospitalized in our Int...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s001340050959
更新日期：1999-08-01 00:00:00
abstract：OBJECTIVE:The Brugada syndrome is a clinical and electrocardiographic familial entity, which may lead to sudden cardiac death. A Brugada pattern ECG may occasionally be caused by conditions such as an overdose of tricyclic antidepressants (TCA). Toxicity of TCA frequently results in the need for critical care support. ...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/s00134-005-0007-3
更新日期：2006-02-01 00:00:00
abstract：OBJECTIVE:The purpose of the study was do describe the architecture of accretions occurring on the tips of central venous catheters (CVC). DESIGN:A conservative procedure was used followed by two different techniques of electron microscopy. SETTING AND PATIENTS:the study included 19 catheters which have been used on ...
journal_title：Intensive care medicine
pub_type： 杂志文章
doi：10.1007/BF01708582
更新日期：1992-01-01 00:00:00
