Ficoll-separated mononuclear cells from sepsis patients are contaminated with granulocytes.


OBJECTIVE:To determine the cell content and purity of Ficoll-separated peripheral blood mononuclear cells and granulocyte isolates in sepsis patients compared to healthy controls. DESIGN AND SETTING:Prospective study in the adult and pediatric intensive care departments of the Erasmus University Medical Center in the Netherlands. PATIENTS:Three sepsis patients (two adults, one child) and four healthy controls. MEASUREMENTS AND RESULTS:Blood leukocytes were separated by Ficoll into an interface and a bottom fraction. The cell content and purity was analyzed by cytospin and flow-cytometric immunofluorescence. In sepsis patients, the interface consisted of 11-52% mononuclear cells only, due to high contamination with granulocytes (48-89%). This was in contrast to a high proportion of mononuclear cells (88-100%) in healthy controls. The bottom fraction showed a cell purity of >or=92% polymorphonuclear granulocytes in sepsis patients as well as in healthy controls. CONCLUSIONS:Ficoll-separated leukocytes of sepsis patients are not suitable for studying mononuclear cells but can be used for studying granulocytes with high purity. The mononuclear cell fraction is highly contaminated with granulocytes. Additional separation techniques are necessary to obtain a pure cell fraction.


Intensive Care Med


Intensive care medicine


van den Akker EL,Baan CC,van den Berg B,Russcher H,Joosten K,Hokken-Koelega AC,Lamberts SW,Koper JW




Has Abstract


2008-05-01 00:00:00












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    abstract:: ...

    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: Hernandez G,Bellomo R,Bakker J

    更新日期:2019-01-01 00:00:00

  • Incidence and circumstances of serum creatinine increase after abdominal aortic surgery.

    abstract:OBJECTIVE:To evaluate the incidence and the circumstances of a moderate increase in serum creatinine early after elective abdominal aortic surgery. DESIGN:Prospective clinical observational study. SETTING:Surgical intensive care unit in a university hospital. PATIENTS:Two hundred and fifteen consecutive adult patien...

    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: Ryckwaert F,Alric P,Picot MC,Djoufelkit K,Colson P

    更新日期:2003-10-01 00:00:00

  • Improvement of cardiac output estimation by the thermodilution method during mechanical ventilation.

    abstract::The reliability of cardiac output estimation by thermodilution during artificial ventilation was studied in anesthetized pigs at the right side of the heart. The estimates exhibited a cyclic modulation related to the ventilation. The amplitude of the modulation was independent of the level of positive end-expiratory p...

    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: Jansen JR,Versprille A

    更新日期:1986-01-01 00:00:00

  • Epidemiological markers of coagulase-negative staphylococci.

    abstract::Several different epidemiological typing methods have been used in studies of coagulase-negative staphylococci; these include biotyping, antibiotic susceptibility pattern analysis, serological typing, phage typing, slime production detection, protein profile analysis, immunoblot fingerprinting and DNA typing. The most...

    journal_title:Intensive care medicine

    pub_type: 杂志文章,评审


    authors: Vandenesch F,Lina B,Lebeau Ch,Greenland TB,Etienne J

    更新日期:1993-01-01 00:00:00

  • Thoracic ultrasonography: a narrative review.

    abstract::This narrative review focuses on thoracic ultrasonography (lung and pleural) with the aim of outlining its utility for the critical care clinician. The article summarizes the applications of thoracic ultrasonography for the evaluation and management of pneumothorax, pleural effusion, acute dyspnea, pulmonary edema, pu...

    journal_title:Intensive care medicine

    pub_type: 杂志文章,评审


    authors: Mayo PH,Copetti R,Feller-Kopman D,Mathis G,Maury E,Mongodi S,Mojoli F,Volpicelli G,Zanobetti M

    更新日期:2019-09-01 00:00:00

  • Dosing of medications in morbidly obese patients in the intensive care unit setting.

    abstract:OBJECTIVE:To derive recommendations for the dosing of commonly used medications in the morbidly obese patient in the ICU. DATA SOURCES:Articles were obtained through computerized searches involving MEDLINE. The bibliographies of retrieved publications and textbooks were reviewed for additional references. STUDY SELEC...

    journal_title:Intensive care medicine

    pub_type: 杂志文章,评审


    authors: Erstad BL

    更新日期:2004-01-01 00:00:00

  • Intensive care management of children following heart and heart-lung transplantation.

    abstract::We report the intensive care management of 23 children (age 3-15 years) following orthotopic heart (HT) and combined heart and lung transplantation (HLT) performed at our 2 institutes between February 1985 and August 1989. Cyclosporin A, azathioprine and steroids were given as routine immunosuppression, whilst anti-th...

    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: Whitehead B,James I,Helms P,Scott JP,Smyth R,Higenbottam TW,McGoldrick J,English TA,Wallwork J,Elliott M

    更新日期:1990-01-01 00:00:00

  • Relation of echocardiographic preload indices to stroke volume in critically ill patients with normal and low cardiac index.

    abstract:OBJECTIVE:To examine the usefulness of preload indices obtained by transoesophageal echocardiography (TOE) for estimating stroke volume at various levels of cardiac index. DESIGN:Prospective clinical study. SETTING:Intensive care unit with surgical patients. PATIENTS:16 ventilated patients monitored via Swan-Ganz ca...

    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: Greim CA,Roewer N,Apfel C,Laux G,Schulte am Esch J

    更新日期:1997-04-01 00:00:00

  • Critical incidents related to invasive mechanical ventilation in the ICU: preliminary descriptive study.

    abstract:OBJECTIVES:To establish a preliminary list of critical incidents (CIs) associated with mechanical ventilation and to describe a CI reporting method. DESIGN:A list of CIs was established based on a consensus among ICU caregivers. The list was compared to CIs collected prospectively during a predefined study period. SE...

    journal_title:Intensive care medicine

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Auriant I,Reignier J,Pibarot ML,Bachat S,Tenaillon A,Raphael JC

    更新日期:2002-04-01 00:00:00

  • Accuracy of plasma neutrophil gelatinase-associated lipocalin in the early diagnosis of contrast-induced acute kidney injury in critical illness.

    abstract:PURPOSE:Neutrophil gelatinase-associated lipocalin (NGAL) is a promising biomarker for acute kidney injury (AKI). We evaluated the diagnostic and prognostic accuracies of plasma NGAL (pNGAL) for contrast-induced AKI (CI-AKI) in critically ill patients. METHODS:In a prospective observational study in two adult intensiv...

    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: Valette X,Savary B,Nowoczyn M,Daubin C,Pottier V,Terzi N,Seguin A,Fradin S,Charbonneau P,Hanouz JL,du Cheyron D

    更新日期:2013-05-01 00:00:00

  • Biomarkers for prediction of renal replacement therapy in acute kidney injury: a systematic review and meta-analysis.

    abstract:PURPOSE:Acute kidney injury (AKI) frequently occurs in critically ill patients and often precipitates use of renal replacement therapy (RRT). However, the ideal circumstances for whether and when to start RRT remain unclear. We performed evidence synthesis of the available literature to evaluate the value of biomarkers...

    journal_title:Intensive care medicine

    pub_type: 杂志文章,评审


    authors: Klein SJ,Brandtner AK,Lehner GF,Ulmer H,Bagshaw SM,Wiedermann CJ,Joannidis M

    更新日期:2018-03-01 00:00:00

  • Measurement of lung volume in mechanically ventilated monkeys with an ultrasonic flow meter and the nitrogen washout method.

    abstract:OBJECTIVE:Measurement of functional residual capacity (FRC) during mechanical ventilation is important to standardise respiratory system compliance and adjust the ventilator settings to optimise lung recruitment. In the present study we compared three methods to measure FRC. DESIGN:The bias flow nitrogen washout techn...

    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: Schibler A,Hammer J,Isler R,Buess C,Newth CJ

    更新日期:2004-01-01 00:00:00

  • Does age affect liver function and the hepatic acute phase response after major abdominal surgery?

    abstract:OBJECTIVE:The liver plays a key role in the inflammatory response during major surgery or infection. The influence of age on liver function and hepatic acute phase protein (APP) synthesis should be serially studied in elderly patients undergoing major abdominal surgery. DESIGN AND SETTING:Prospective and descriptive s...

    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: Suttner SW,Sürder C,Lang K,Piper SN,Kumle B,Boldt J

    更新日期:2001-11-01 00:00:00

  • Long-term mortality in patients with chronic obstructive pulmonary disease following extracorporeal membrane oxygenation for cardiac assist after cardiovascular surgery.

    abstract:PURPOSE:Information on predisposing risk factors influencing long-term survival after extracorporeal membrane oxygenation (ECMO) support remains scarce. In critically ill patients chronic obstructive pulmonary disease (COPD) is an independent risk factor for mortality and morbidity. We assessed the influence of COPD on...

    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: Distelmaier K,Niessner A,Haider D,Lang IM,Heinz G,Maurer G,Koinig H,Steinlechner B,Goliasch G

    更新日期:2013-08-01 00:00:00

  • Lung water assessment by lung ultrasonography in intensive care: a pilot study.

    abstract:OBJECTIVE:To investigate the accuracy of lung ultrasonography (LUS) in the quantification of lung water in critically ill patients by using quantitative computed tomography (CT) as the gold standard for the determination of lung weight. METHODS:Twenty consecutive patients admitted to an intensive care unit who underwe...

    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: Baldi G,Gargani L,Abramo A,D'Errico L,Caramella D,Picano E,Giunta F,Forfori F

    更新日期:2013-01-01 00:00:00

  • The impact of early goal-directed fluid management on survival in an experimental model of severe acute pancreatitis.

    abstract:PURPOSE:Severe acute pancreatitis (SAP) remains a life-threatening disease with classic etiology of systemic inflammatory response and mortality between 30 and 50 %. The aim of the present study is to compare two different treatment strategies of goal-directed hemodynamic management and evaluate their impact on surviva...

    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: Trepte CJ,Bachmann KA,Stork JH,Friedheim TJ,Hinsch A,Goepfert MS,Mann O,Izbicki JR,Goetz AE,Reuter DA

    更新日期:2013-04-01 00:00:00

  • Vitamin therapy in critically ill patients: focus on thiamine, vitamin C, and vitamin D.

    abstract:: ...

    journal_title:Intensive care medicine

    pub_type: 杂志文章,评审


    authors: Amrein K,Oudemans-van Straaten HM,Berger MM

    更新日期:2018-11-01 00:00:00

  • The application of potassium selective electrodes in the intensive care unit.

    abstract::Recent advances in analytical electrochemistry have provided highly selective potassium ion exchangers and robust polymeric membranes that can be used to measure the potassium activity in plasma or whole blood. The relationship between the activity measurement and the more familiar concentration measurement is explore...

    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: Treasure T

    更新日期:1978-04-01 00:00:00

  • Torsade de pointes as a complication of brainstem encephalitis.

    abstract::A case of brainstem encephalitis complicated by torsade de pointes is described. The possible occurrence of ventricular arrhythmias may contribute to the mortality in this condition. We recommend the admission of patients with brainstem encephalitis to an intensive care unit, for a period of electrocardiographic monit...

    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: De Keyser J,De Boel S,Ceulemans L,Ebinger G

    更新日期:1987-01-01 00:00:00

  • A brief history of tracheostomy and tracheal intubation, from the Bronze Age to the Space Age.

    abstract:OBJECTIVE:To present a concise history of tracheostomy and tracheal intubation for the approximately forty centuries from their earliest description around 2000 BC until the middle of the twentieth century, at which time a proliferation of advances marked the beginning of the modern era of anesthesiology. DATA SOURCES...

    journal_title:Intensive care medicine

    pub_type: 历史文章,杂志文章


    authors: Szmuk P,Ezri T,Evron S,Roth Y,Katz J

    更新日期:2008-02-01 00:00:00

  • Nosocomial sinusitis with isolation of anaerobic bacteria in ICU patients.

    abstract:OBJECTIVE:To determine the frequency and the eventual clinical characteristics of nosocomial sinusitis with anaerobic bacteria isolation in patients in the intensive care unit (ICU). DESIGN:Retrospective study. SETTING:A 12-bed medical ICU in a teaching hospital. PATIENTS:30 adult patients with documented nosocomial...

    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: Le Moal G,Lemerre D,Grollier G,Desmont C,Klossek JM,Robert R

    更新日期:1999-10-01 00:00:00

  • Ventilator-associated pneumonia in children after cardiac surgery in The Netherlands.

    abstract:PURPOSE:We conducted a retrospective cohort study in an academic tertiary care center to characterize ventilator-associated pneumonia (VAP) in pediatric patients after cardiac surgery in The Netherlands. METHODS:All patients following cardiac surgery and mechanically ventilated for ≥24 h were included. The primary out...

    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: Roeleveld PP,Guijt D,Kuijper EJ,Hazekamp MG,de Wilde RB,de Jonge E

    更新日期:2011-10-01 00:00:00

  • The influence of infection sites on development and mortality of ARDS.

    abstract:OBJECTIVE:Infection is the most frequent cause of acute respiratory distress syndrome (ARDS). However, little is known about the influence of infection sites on ARDS. This study aimed to assess the associations of infection sites with ARDS development and mortality in critically ill infected patients. DESIGN:Prospecti...

    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: Sheu CC,Gong MN,Zhai R,Bajwa EK,Chen F,Thompson BT,Christiani DC

    更新日期:2010-06-01 00:00:00

  • Autoregulation of ventilation with neurally adjusted ventilatory assist on extracorporeal lung support.

    abstract:PURPOSE:Extracorporeal membrane oxygenation (ECMO) can support oxygenation and carbon dioxide elimination in severe lung failure. Usually it is accompanied by controlled mechanical ventilation. Neurally adjusted ventilatory assist (NAVA) is a new mode of ventilation triggered by the diaphragmatic electrical activity an...

    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: Karagiannidis C,Lubnow M,Philipp A,Riegger GA,Schmid C,Pfeifer M,Mueller T

    更新日期:2010-12-01 00:00:00

  • Decontamination of the digestive tract and oropharynx: hospital acquired infections after discharge from the intensive care unit.

    abstract:OBJECTIVE:To determine the incidence rates of hospital acquired infections (HAI) during the first 14 days after ICU discharge after treatment during ICU-stay with Selective Decontamination of the Digestive tract (SDD), Selective Oropharyngeal Decontamination (SOD) or Standard Care (SC). DESIGN:Prospective observationa...

    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: de Smet AM,Hopmans TE,Minderhoud AL,Blok HE,Gossink-Franssen A,Bernards AT,Bonten MJ

    更新日期:2009-09-01 00:00:00

  • Procalcitonin for early diagnosis and differentiation of SIRS, sepsis, severe sepsis, and septic shock.

    abstract:OBJECTIVE:To determine the value of procalcitonin (PCT) in the early diagnosis (and differentiation) of patients with SIRS, sepsis, severe sepsis, and septic shock in comparison to C-reactive protein (CRP), white blood cell and thrombocyte count, and APACHE-II score (AP-II). DESIGN:Prospective cohort study including a...

    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: Brunkhorst FM,Wegscheider K,Forycki ZF,Brunkhorst R

    更新日期:2000-03-01 00:00:00

  • Describing ICU data with tables.

    abstract:INTRODUCTION:The purpose of a scientific paper is to communicate results and within the paper this applies especially to the presentation of data. It is the universal practice in medical journals to present statistical results using tables. Good tables are an integral part of the manuscript. To help researchers communi...

    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: Alberti C,Boulkedid R

    更新日期:2014-05-01 00:00:00

  • Research priorities in critical care medicine in the UK.

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    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: Goldfrad C,Vella K,Bion JF,Rowan KM,Black NA

    更新日期:2000-10-01 00:00:00

  • Non-operative management of gastric perforation secondary to cardiopulmonary resuscitation.

    abstract::We report the case of a 72-year-old male who suffered a cardiac arrest during an early positive treadmill stress test. After successful resuscitation the patient had evidence of a gastric perforation. Because of his hemodynamic stability, lack of peritoneal signs, and prohibitively high surgical risk, a non-operative ...

    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: Low LL,Ripple GR,Bruderer BP,Harrington GR

    更新日期:1994-07-01 00:00:00

  • Acute kidney injury in critically ill patients with 2009 influenza A (H1N1) viral pneumonia: an observational study.

    abstract:OBJECTIVE:To describe the incidence, risk factors, and impact on mortality of acute kidney injury (AKI) in patients with 2009 influenza A (H1N1) viral pneumonia requiring mechanical ventilation. DESIGN:Observational cohort study. PATIENTS AND METHODS:AKI was defined as risk, injury or failure, according to the RIFLE ...

    journal_title:Intensive care medicine

    pub_type: 杂志文章


    authors: Nin N,Lorente JA,Soto L,Ríos F,Hurtado J,Arancibia F,Ugarte S,Echevarría E,Cardinal P,Saldarini F,Bagnulo H,Cortés I,Bujedo G,Ortega C,Frutos F,Esteban A

    更新日期:2011-05-01 00:00:00