Avocado soybean unsaponifiables (ASU) suppress TNF-alpha, IL-1beta, COX-2, iNOS gene expression, and prostaglandin E2 and nitric oxide production in articular chondrocytes and monocyte/macrophages.


OBJECTIVE:To evaluate the effects of avocado soybean unsaponifiables (ASU) on proinflammatory mediators in chondrocytes and monocyte/macrophage-like cells. DESIGN:To determine the dose response of ASU, chondrocytes (5 x 10(5) cells/well) were incubated at 5% CO(2), 37 degrees C for 72 h with (1) control media alone or (2) ASU at concentrations of 0.3, 0.9, 2.7, 8.3, and 25 microg/ml. Cells were activated with 20 ng/ml lipopolysaccharide (LPS) for 24 h and cell supernatants were analyzed for prostaglandin E(2) (PGE(2)) and nitrite content. Chondrocytes and THP-1 monocyte/macrophages (5 x 10(5) cells/well) were incubated at 5% CO(2), 37 degrees C for 72 h with (1) control media alone or (2) ASU (25 mug/ml). One set of cells was activated for 1 h with LPS (20 ng/ml) for both reverse-transcriptase PCR and real-time PCR analysis of tumor necrosis factor-alpha (TNF-alpha), interleukin-1-beta (IL-1beta), cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) expression. One set of cells was activated for 24 h to analyze secreted PGE(2) and nitrite levels in the cellular supernatant. RESULTS:ASU reduced TNF-alpha, IL-1beta, COX-2, and iNOS expression in LPS-activated chondrocytes to levels similar to nonactivated control levels. The suppression of COX-2 and iNOS expression was paralleled by a significant reduction in PGE(2) and nitrite, respectively, in the cellular supernatant. ASU also reduced TNF-alpha and IL-1beta expression in LPS-activated monocyte/macrophage-like cells. CONCLUSION:The present study demonstrates that the anti-inflammatory activity of ASU is not restricted to chondrocytes, but also affects monocyte/macrophage-like cells that serve as a prototype for macrophages in the synovial membrane. These observations provide a scientific rationale for the pain-reducing and anti-inflammatory effects of ASU observed in osteoarthritis patients.


Au RY,Al-Talib TK,Au AY,Phan PV,Frondoza CG




Has Abstract


2007-11-01 00:00:00














  • The impact of early intra-articular administration of interleukin-1 receptor antagonist on lubricin metabolism and cartilage degeneration in an anterior cruciate ligament transection model.

    abstract:OBJECTIVE:Study the impact of intra-articular interleukin-1 receptor antagonist (IL-1 ra) treatment on lubricin biosynthesis following anterior cruciate ligament transection (ACLT) in the rat and evaluate the effect of combined IL-1 ra and recombinant human lubricin (rhPRG4) treatments on chondrocyte apoptosis. METHOD...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Elsaid KA,Zhang L,Shaman Z,Patel C,Schmidt TA,Jay GD

    更新日期:2015-01-01 00:00:00

  • Upregulation of lipocalin-2 (LCN2) in osteoarthritic cartilage is not necessary for cartilage destruction in mice.

    abstract:OBJECTIVE:Lipocalin-2 (LCN2) is a recently characterized adipokine that is upregulated in chondrocytes treated with pro-inflammatory mediators and in the synovial fluid of osteoarthritis (OA) patients. Here, we explored the in vivo functions of LCN2 in OA cartilage destruction in mice. METHODS:The expression levels of...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Choi WS,Chun JS

    更新日期:2017-03-01 00:00:00

  • Studies of pain management in osteoarthritis: bedside to policy.

    abstract:OBJECTIVE:Osteoarthritis (OA) is a debilitating chronic condition requiring long-term treatment of pain and functional impairment. Our objective was to characterize studies addressing management of OA-related pain with respect to the breadth of interventions, trial duration and size, outcome measures, and funding sourc...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,meta分析


    authors: Losina E,Dervan EE,Daigle ME,Katz JN

    更新日期:2013-09-01 00:00:00

  • Establishment of a bipotent cell line CL-1 which differentiates into chondrocytes and adipocytes from adult mouse.

    abstract:OBJECTIVE:Establishment of a clonal bipotent chondroprogenitor cell line from adult mouse to provide a new tool for the elucidation of chondrogenesis in adult animal. DESIGN:A clonal cell line CL-1 was established from tibia of adult mouse. Differentiation of CL-1 was characterized in monolayer culture. Effects of gro...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Kitamura H

    更新日期:2004-01-01 00:00:00

  • Diffusion of Gd-DTPA²⁻ into articular cartilage.

    abstract:OBJECTIVES:The delayed Gadolinium-Enhanced MRI of Cartilage (dGEMRIC) technique is a method proposed for non-invasive measurement of cartilage glycosaminoglycan (GAG) content. In this method, gadopentetate (Gd-DTPA²⁻) is assumed to distribute in cartilage in inverse relation to the GAG distribution, thus allowing quant...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Salo EN,Nissi MJ,Kulmala KA,Tiitu V,Töyräs J,Nieminen MT

    更新日期:2012-02-01 00:00:00

  • Osteoarthritis year 2010 in review: pathomechanisms.

    abstract::Osteoarthritis is characterized by progressive breakdown of articular cartilage. This review summarizes findings of the last year, which shed new light on mechanisms and factors involved in cartilage loss. Evidence is accumulating that the transcription factor hypoxia-inducible factor-2α (HIF-2α) is highly enhanced in...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: van den Berg WB

    更新日期:2011-04-01 00:00:00

  • C-reactive protein as a biomarker of emergent osteoarthritis.

    abstract:OBJECTIVE:We evaluated C-reactive protein (C-RP), a quantitative marker of the acute phase response, as a potential biomarker of prevalent and incident osteoarthritis of the knee (OAK). METHODS:Serum C-reactive protein concentrations were characterized with ultrasensitive rate nephelometry in a population-based sample...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Sowers M,Jannausch M,Stein E,Jamadar D,Hochberg M,Lachance L

    更新日期:2002-08-01 00:00:00

  • Cartilage degeneration in different human joints.

    abstract::Variations among joints in the initiation and progression of degeneration may be explained, in part, by metabolic, biochemical and biomechanical differences. Compared to the cartilage in the knee joint, ankle cartilage has a higher content of proteoglycans and water, as well as an increased rate of proteoglycan turnov...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Kuettner KE,Cole AA

    更新日期:2005-02-01 00:00:00

  • Reinforcement of articular cartilage with a tissue-interpenetrating polymer network reduces friction and modulates interstitial fluid load support.

    abstract:OBJECTIVE:Osteoarthritis (OA) is associated with increased articular cartilage hydraulic permeability and decreased maintenance of high interstitial fluid load support (IFLS) during articulation, resulting in increased friction on the cartilage solid matrix. This study assesses frictional response following in situ syn...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Cooper BG,Lawson TB,Snyder BD,Grinstaff MW

    更新日期:2017-07-01 00:00:00

  • Articular cartilage repair using a tissue-engineered cartilage-like implant: an animal study.

    abstract:OBJECTIVE:Because articular cartilage has limited ability to repair itself, treatment of (osteo)chondral lesions remains a clinical challenge. We aimed to evaluate how well a tissue-engineered cartilage-like implant, derived from chondrocytes cultured in a novel patented, scaffold-free bioreactor system, would perform ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Mainil-Varlet P,Rieser F,Grogan S,Mueller W,Saager C,Jakob RP

    更新日期:2001-01-01 00:00:00

  • Differential expression of interleukin-17 and interleukin-22 in inflamed and non-inflamed synovium from osteoarthritis patients.

    abstract:OBJECTIVE:Synovitis associated with osteoarthritis (OA) is directly responsible for several clinical symptoms and reflects OA's structural progression. This study sought to analyze the expression of proinflammatory mediations, including Interleukin (IL)-17 and IL-22, which play key roles in regulating inflammatory proc...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Deligne C,Casulli S,Pigenet A,Bougault C,Campillo-Gimenez L,Nourissat G,Berenbaum F,Elbim C,Houard X

    更新日期:2015-11-01 00:00:00

  • Should subchondral bone turnover be targeted when treating osteoarthritis?

    abstract:OBJECTIVE:Osteoarthritis (OA) is the most common form of arthritic disease, and it is a major cause of disability and impaired quality of life in the elderly. OA is a complex disease of the entire joint, including bone and cartilage, thereby presenting alternative approaches for treatment. This review summarizes emergi...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,评审


    authors: Karsdal MA,Leeming DJ,Dam EB,Henriksen K,Alexandersen P,Pastoureau P,Altman RD,Christiansen C

    更新日期:2008-06-01 00:00:00

  • Histopathology atlas of animal model systems - overview of guiding principles.

    abstract::Animal model systems represent an important adjunct and surrogate for studies of osteoarthritis (OA) in humans. They provide a means to study OA pathophysiology as well as aid in the development of therapeutic agents and biological markers for diagnosing and prognosing the disease. Thus, it is of great importance for ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Aigner T,Cook JL,Gerwin N,Glasson SS,Laverty S,Little CB,McIlwraith W,Kraus VB

    更新日期:2010-10-01 00:00:00

  • Dynamic knee loading is related to cartilage defects and tibial plateau bone area in medial knee osteoarthritis.

    abstract:OBJECTIVE:To evaluate the relationship between dynamic mechanical loading, as indicated by external knee adduction moment (KAM) measures during walking, and measures of articular cartilage morphology and subchondral bone size in people with medial knee osteoarthritis (OA). DESIGN:180 individuals with radiographic medi...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Creaby MW,Wang Y,Bennell KL,Hinman RS,Metcalf BR,Bowles KA,Cicuttini FM

    更新日期:2010-11-01 00:00:00

  • In vivo serial joint space measurements during dynamic loading in a canine model of osteoarthritis.

    abstract:OBJECTIVE:To devise a reliable, sensitive method to measure joint space in vivo during dynamic loading. Additionally, to determine if dynamic joint space changes were related to the severity of long-term cartilage damage. DESIGN:Subjects were 23 adult foxhounds (18 experimental, 5 control). Experimental subjects had s...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Anderst WJ,Les C,Tashman S

    更新日期:2005-09-01 00:00:00

  • Articular damage caused by metal plugs in a rabbit model for treatment of localized cartilage defects.

    abstract:OBJECTIVE:Currently, the surgical treatment of localized cartilage defects has limitations. Alternatively, localized cartilage defects may be treated with small biocompatible metal cartilage tacks. Our purpose was to investigate the applicability of defect-size femoral implants. Different bearing materials, cobalt-chro...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Custers RJ,Dhert WJ,van Rijen MH,Verbout AJ,Creemers LB,Saris DB

    更新日期:2007-08-01 00:00:00

  • Femoral trabecular bone of osteoarthritic and normal subjects in an age and sex matched group.

    abstract:OBJECTIVE:To describe changes to the cancellous structure of femoral bone from patients with severe primary osteoarthritis by comparison with age and sex matched controls. METHOD:Specimens were taken from 18 male and 18 female pairs. One of each pair was a normal control, the other having severe primary osteoarthritis...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Fazzalari NL,Parkinson IH

    更新日期:1998-11-01 00:00:00

  • Optical clearing in collagen- and proteoglycan-rich osteochondral tissues.

    abstract:OBJECTIVE:Recent developments in optical clearing and microscopy technology have enabled the imaging of intact tissues at the millimeter scale to characterize cells via fluorescence labeling. While these techniques have facilitated the three-dimensional (3D) cellular characterization within brain and heart, study of de...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Neu CP,Novak T,Gilliland KF,Marshall P,Calve S

    更新日期:2015-03-01 00:00:00

  • Increased rate of hysterectomy in women undergoing surgery for osteoarthritis of the knee.

    abstract::We performed a case-controlled study to determine whether previous gynecological surgery is associated with severe osteoarthritis (OA) of the knee, the hip or the spine, as well as mild OA of the knee. Patients who underwent surgical treatment for OA were defined as having severe OA. Patients with knee pain and radiog...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Inoue K,Ushiyama T,Kim Y,Shichikawa K,Nishioka J,Hukuda S

    更新日期:1995-09-01 00:00:00

  • A randomized trial of patellofemoral bracing for treatment of patellofemoral osteoarthritis.

    abstract:PURPOSE:The number of effective knee osteoarthritis (OA) interventions, especially those tailored to specific compartmental involvement, are small. The objective of this study was to determine the efficacy of a realigning patellofemoral (PF) brace in improving pain and function among persons with symptomatic lateral PF...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,随机对照试验


    authors: Hunter DJ,Harvey W,Gross KD,Felson D,McCree P,Li L,Hirko K,Zhang B,Bennell K

    更新日期:2011-07-01 00:00:00

  • Oxygen and pH-sensitivity of human osteoarthritic chondrocytes in 3-D alginate bead culture system.

    abstract:OBJECTIVE:To identify the effect of alterations in physical parameters such as oxygen and pH on processes associated with cellular redox balance in osteoarthritic chondrocytes. METHOD:Human osteoarthritic chondrocytes (HOAC) were isolated from total knee arthroplasty samples and cultured in 3-D alginate beads in four ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Collins JA,Moots RJ,Winstanley R,Clegg PD,Milner PI

    更新日期:2013-11-01 00:00:00

  • Culture expanded primary chondrocytes have potent immunomodulatory properties and do not induce an allogeneic immune response.

    abstract:OBJECTIVE:Allogeneic cell therapies, such as mesenchymal stromal cells (MSC), which have potent regenerative and anti-inflammatory potential are being investigated as a therapy for osteoarthritis (OA) and cartilage injury. Here we describe another potential source of regenerative and anti-inflammatory allogeneic cells,...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Lohan P,Treacy O,Lynch K,Barry F,Murphy M,Griffin MD,Ritter T,Ryan AE

    更新日期:2016-03-01 00:00:00

  • Relationship between weight loss in obese knee osteoarthritis patients and serum biomarkers of cartilage breakdown: secondary analyses of a randomised trial.

    abstract:OBJECTIVE:To explore effects of weight loss and maintenance on serum cartilage biomarkers denaturation neoepitope for Collagen2 (Coll2-1) and Fibulin3 fragment (Fib3-2), as well as correlations between Coll2-1 and Fib3-2 and symptomatic improvement, in a knee osteoarthritis (KOA) population. DESIGN:192 obese KOA patie...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章,随机对照试验


    authors: Bartels EM,Henrotin Y,Bliddal H,Centonze P,Henriksen M

    更新日期:2017-10-01 00:00:00

  • Correlation between the MR T2 value at 4.7 T and relative water content in articular cartilage in experimental osteoarthritis induced by ACL transection.

    abstract:OBJECTIVE:Both animal and human studies using magnetic resonance imaging (MRI) show that cartilage degeneration increases the T2 value. However, it is unclear whether the T2 value correlates linearly with water content in cartilage with osteoarthritis. The purpose of this study was to investigate the relationship betwe...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Chou MC,Tsai PH,Huang GS,Lee HS,Lee CH,Lin MH,Lin CY,Chung HW

    更新日期:2009-04-01 00:00:00

  • Reliability, reproducibility and variability of the traditional Histologic/Histochemical Grading System vs the new OARSI Osteoarthritis Cartilage Histopathology Assessment System.

    abstract:OBJECTIVE:For many years, the Histologic/Histochemical Grading System (HHGS) for osteoarthritis monitoring has been used as a histological scoring system for the quality of cartilage. There are, however, some limitations using this grading system. The goal of the investigation presented in this paper was to examine the...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Custers RJ,Creemers LB,Verbout AJ,van Rijen MH,Dhert WJ,Saris DB

    更新日期:2007-11-01 00:00:00

  • Cross-sectional and longitudinal associations between radiographic features and measures of pain and physical function in hand osteoarthritis.

    abstract:OBJECTIVE:Previous longitudinal studies have shown no associations between increasing amount of radiographic hand osteoarthritis (OA) and levels of hand pain/disability. In this longitudinal study, we aimed to study whether radiographic hand OA was related to pain/disability in cross-sectional and longitudinal settings...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Haugen IK,Slatkowsky-Christensen B,Bøyesen P,van der Heijde D,Kvien TK

    更新日期:2013-09-01 00:00:00

  • Knee osteoarthritis patients with severe nocturnal pain have altered proximal tibial subchondral bone mineral density.

    abstract:OBJECTIVE:Our objective was to investigate relationships between proximal tibial subchondral bone mineral density (BMD) and nocturnal pain in patients with knee osteoarthritis (OA). METHODS:The preoperative knee of 42 patients booked for knee arthroplasty was scanned using quantitative computed tomography (QCT). Pain ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Burnett WD,Kontulainen SA,McLennan CE,Hazel D,Talmo C,Hunter DJ,Wilson DR,Johnston JD

    更新日期:2015-09-01 00:00:00

  • Effect of hyaluronic acid (MW 500-730 kDa) on proteoglycan and nitric oxide production in human osteoarthritic chondrocyte cultures exposed to hydrostatic pressure.

    abstract:OBJECTIVE:This study investigated the in vitro effects of hyaluronic acid (HA) of molecular weight (MW) 500-730 kDa on human articular chondrocytes cultivated for 48 h in the presence of interleukin-1beta (IL-1beta) with and without hydrostatic cyclical pressure. DESIGN:The effects of 10 and 100 microg/ml HA with and ...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Fioravanti A,Cantarini L,Chellini F,Manca D,Paccagnini E,Marcolongo R,Collodel G

    更新日期:2005-08-01 00:00:00

  • Bisphosphonates reduce bone mineral loss at ligament entheses after joint injury.

    abstract:OBJECTIVE:To examine the effects of anterior cruciate ligament (ACL) insufficiency, and subsequent bisphosphonate (BP) antiresorptive therapy, on the bone mineral interface at the enthesis of remaining ligamentous restraints. METHODS:We measured bone mineral geometry (and subsequent adaptation) at the medial collatera...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Doschak MR,LaMothe JM,Cooper DM,Hallgrimsson B,Hanley DA,Bray RC,Zernicke RF

    更新日期:2005-09-01 00:00:00

  • Altered cartilage mechanics and histology in knee osteoarthritis: relation to clinical assessment (ICRS Grade).

    abstract:OBJECTIVE:Substantial changes in articular cartilage composition and mechanical properties occur during the development of osteoarthritis (OA). While softening in the initial stage is reported and sometimes used as an indicator of early OA, there is a lack of data relating the macroscopic appearance of cartilage to its...

    journal_title:Osteoarthritis and cartilage

    pub_type: 杂志文章


    authors: Kleemann RU,Krocker D,Cedraro A,Tuischer J,Duda GN

    更新日期:2005-11-01 00:00:00