当前位置： SCI文献检索 > OSTEOARTHRITIS AND CARTILAGE期刊下所有文献 > Methylation of the OP-1 promoter: potential role in the age-related decline in OP-1 expression in cartilage.

Methylation of the OP-1 promoter: potential role in the age-related decline in OP-1 expression in cartilage.

Abstract：

OBJECTIVE:An age-related decline in chondrocyte production of osteogenic protein-1 (OP-1) (Bone Morphogenetic Protein-7) may contribute to cartilage loss in osteoarthritis. This study was designed to determine if increased methylation of the OP-1 promoter might serve as a mechanism for the age-related decline in OP-1 expression. METHODS:Human articular chondrocytes were isolated from cartilage obtained after death from tissue donors (ages 19-86 years) without a known history of arthritis. DNA was obtained from isolated chondrocytes in primary culture and analyzed for OP-1 promoter methylation by polymerase chain reaction (PCR) after bisulfite treatment. Cultured cells were treated with the DNA methyltransferase inhibitor 5-azacytidine and OP-1 production was measured in the media by enzyme-linked immunosorbent assay (ELISA). RNA was isolated to measure expression of insulin-like growth factor-1 (IGF-1), the IGF-1 receptor (IGF-1R), aggrecan, and OP-1 by real-time PCR. RESULTS:Methylation of the OP-1 promoter was detected in chondrocytes isolated from tissue obtained from older adults and there was a positive correlation between age and OP-1 methylation status (n=22, R(2)=0.277, P=0.014). Inhibition of methylation in cultured cells with 5-azacytidine increased chondrocyte production of OP-1 protein and increased the expression of the IGF-1, the IGF-1R, aggrecan, and OP-1 genes but not GAPDH. CONCLUSION:Age-related methylation of the OP-1 promoter may contribute to a decrease in OP-1 production in cartilage and a decrease in expression of OP-1 responsive genes such as IGF-1, the IGF-1R, and aggrecan.

journal_name

Osteoarthritis Cartilage

journal_title

Osteoarthritis and cartilage

authors

Loeser RF,Im HJ,Richardson B,Lu Q,Chubinskaya S

doi

10.1016/j.joca.2008.08.003

subject

Has Abstract

pub_date

2009-04-01 00:00:00

pages

513-7

issue

4

eissn

1063-4584

issn

1522-9653

pii

S1063-4584(08)00292-6

journal_volume

17

pub_type

杂志文章

相关文献

OSTEOARTHRITIS AND CARTILAGE文献大全
  • Cartilage loss in radiographically normal knees depends on radiographic status of the contralateral knee - data from the Osteoarthritis Initiative.

    abstract：OBJECTIVE:To test whether radiographically normal knees with contralateral radiographic knee osteoarthritis (OA), but without contralateral trauma history, display greater cartilage thickness loss than knees from subjects with bilaterally radiographically normal knees. METHODS:828 radiographically normal knees (Kellgr...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2018.10.006

    authors： Eckstein F,Maschek S,Roemer FW,Duda GN,Sharma L,Wirth W

    更新日期：2019-02-01 00:00:00

  • A dominant TRPV4 variant underlies osteochondrodysplasia in Scottish fold cats.

    abstract：OBJECTIVE:Scottish fold cats, named for their unique ear shape, have a dominantly inherited osteochondrodysplasia involving malformation in the distal forelimbs, distal hindlimbs and tail, and progressive joint destruction. This study aimed to identify the gene and the underlying variant responsible for the osteochondr...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2016.03.019

    authors： Gandolfi B,Alamri S,Darby WG,Adhikari B,Lattimer JC,Malik R,Wade CM,Lyons LA,Cheng J,Bateman JF,McIntyre P,Lamandé SR,Haase B

    更新日期：2016-08-01 00:00:00

  • Gene expression profiling following BMP-2 induction of mesenchymal chondrogenesis in vitro.

    abstract：OBJECTIVE:This study aims to apply gene expression profiling technology to gain insight into the molecular regulation of mesenchymal chondrogenesis. METHODS:The experimental system consists of micromass cultures of C3H10T1/2 cells, a murine multipotential embryonic cell line, treated with the chondroinductive growth f...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1053/joca.2001.0478

    authors： Izzo MW,Pucci B,Tuan RS,Hall DJ

    更新日期：2002-01-01 00:00:00

  • Dimensionality and clinical importance of pain and disability in hand osteoarthritis: Development of the Australian/Canadian (AUSCAN) Osteoarthritis Hand Index.

    abstract：OBJECTIVE:To develop a reliable, valid, and responsive self-administered questionnaire to probe pain, stiffness and physical disability in patients with osteoarthritis (OA) of the hand. DESIGN:In order to assess the dimensionality of the symptomatology of hand OA, a self-administered questionnaire was developed to pro...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1053/joca.2002.0837

    authors： Bellamy N,Campbell J,Haraoui B,Buchbinder R,Hobby K,Roth JH,MacDermid JC

    更新日期：2002-11-01 00:00:00

  • Identification and pharmacological characterization of a novel inhibitor of autotaxin in rodent models of joint pain.

    abstract：OBJECTIVE:Autotaxin is a secreted lysophospholipase that mediates the conversion of lysophosphatidyl choline (LPC) to lysophosphatidic acid (LPA), a bioactive lipid mediator. Autotaxin levels in plasma and synovial fluid correlate with disease severity in patients with knee osteoarthritis (OA). The goal of this study w...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2016.09.006

    authors： Thirunavukkarasu K,Swearingen CA,Oskins JL,Lin C,Bui HH,Jones SB,Pfeifer LA,Norman BH,Mitchell PG,Chambers MG

    更新日期：2017-06-01 00:00:00

  • The response of elderly human articular cartilage to mechanical stimuli in vitro.

    abstract：OBJECTIVE:To investigate the biosynthetic response of elderly human femoral head articular cartilage to mechanical stimulation in vitro and its variation with site. METHOD:Full-depth cartilage biopsies of articular cartilage were removed from defined sites on 10 femoral heads from patients aged 68-95 years. Cartilage ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2005.07.002

    authors： Plumb MS,Aspden RM

    更新日期：2005-12-01 00:00:00

  • Premature osteoarthritis in the Disproportionate micromelia (Dmm) mouse.

    abstract：OBJECTIVE:Degeneration of articular cartilage leads to the development of osteoarthritis (OA), but the molecular pathology of the disease is poorly understood. The Disproportionate micromelia (Dmm) mouse has a deletion mutation in the C-propeptide encoding region of Col2a1, which leads to a defective cartilage matrix. ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2005.11.011

    authors： Bomsta BD,Bridgewater LC,Seegmiller RE

    更新日期：2006-05-01 00:00:00

  • Association between smoking and risk of knee osteoarthritis: a systematic review and meta-analysis.

    abstract：OBJECTIVE:To investigate the association between smoking and the risk for knee osteoarthritis (OA). DESIGN:Cohort, case-control, and cross-sectional studies were obtained from the Medline, Embase, and Web of Science databases. Estimates were calculated using a random-effects model. Subgroup analyses and meta-regressio...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,meta分析,评审

    doi：10.1016/j.joca.2016.12.020

    authors： Kong L,Wang L,Meng F,Cao J,Shen Y

    更新日期：2017-06-01 00:00:00

  • Phase-sensitive X-ray imaging of synovial joints.

    abstract：OBJECTIVE:To test the efficacy of phase-sensitive X-ray imaging for intact synovial joints, whereby refraction effects, along with the attenuation of conventional radiography, can be exploited. DESIGN:Intact cadaveric human knee joints were imaged, in the computed tomographic mode, using an analyzer-based X-ray system...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2009.03.005

    authors： Li J,Zhong Z,Connor D,Mollenhauer J,Muehleman C

    更新日期：2009-09-01 00:00:00

  • Novel biomarkers of intervertebral disc cells and evidence of stem cells in the intervertebral disc.

    abstract：OBJECTIVE:Rat intervertebral disc (IVD) is one of the most commonly used and cost-effective alternative models for human IVD. Many IVD related clinical studies need to be pre-tested on rat IVDs. However, studies on the heterogeneous cell clusters of the rat IVD are inadequate, and a further understanding of the marker ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2020.12.005

    authors： Wang J,Huang Y,Huang L,Shi K,Wang J,Zhu C,Li L,Zhang L,Feng G,Liu L,Song Y

    更新日期：2020-12-15 00:00:00

  • The rs4238326 polymorphism in ALDH1A2 gene potentially associated with non-post traumatic knee osteoarthritis susceptibility: a two-stage population-based study.

    abstract：OBJECTIVE:A recent genome-wide association study reported significant associations of genetic variants within the ALDH1A2 gene with osteoarthritis (OA) of the hand in European populations. However, these findings have not been well generalized to other joints, or to other populations. METHODS:We performed a two-stage ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2017.01.003

    authors： Chu M,Zhu X,Wang C,Rong J,Wang Y,Wang S,Xing B,Tao Y,Zhuang X,Jiang L

    更新日期：2017-07-01 00:00:00

  • The Bristol 'OA500' study: progression of osteoarthritis (OA) over 3 years and the relationship between clinical and radiographic changes at the knee joint.

    abstract：:Five hundred patients with established, symptomatic limb joint OA have been recruited into an on-going prospective study of the natural history of the condition. Four hundred and fifteen patients (mean age 65.6 years, female to male ratio 2.05:1) were available for a full clinical and radiographic review 3 years after...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/s1063-4584(97)80002-7

    authors： Dieppe PA,Cushnaghan J,Shepstone L

    更新日期：1997-03-01 00:00:00

  • Detection of changes in cartilage water content using MRI T2-mapping in vivo.

    abstract：OBJECTIVES:Osteoarthritis (OA) is the most prevalent chronic disease in the elderly, and it is generally diagnosed at an advanced state when treatment is difficult if not impossible. The early form of OA is characterized by an elevated water content in the cartilage tissue. The purpose of this study was to verify in vi...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1053/joca.2002.0847

    authors： Liess C,Lüsse S,Karger N,Heller M,Glüer CC

    更新日期：2002-12-01 00:00:00

  • Human autologous culture expanded bone marrow mesenchymal cell transplantation for repair of cartilage defects in osteoarthritic knees.

    abstract：OBJECTIVE:There is no widely accepted method to repair articular cartilage defects. Bone marrow mesenchymal cells have the potential to differentiate into bone, cartilage, fat and muscle. Bone marrow mesenchymal cell transplantation is easy to use clinically because cells can be easily obtained and can be multiplied wi...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1053/joca.2001.0504

    authors： Wakitani S,Imoto K,Yamamoto T,Saito M,Murata N,Yoneda M

    更新日期：2002-03-01 00:00:00

  • The groove model of osteoarthritis applied to the ovine fetlock joint.

    abstract：OBJECTIVE:Until now there have been no appropriate models for metacarpophalangeal osteoarthritis (OA), even though OA in this joint is a significant medical and economic problem in horses. A good model would be useful to evaluate progression and treatment of OA, particularly in this joint. Therefore, we translated the ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2007.11.010

    authors： Mastbergen SC,Pollmeier M,Fischer L,Vianen ME,Lafeber FP

    更新日期：2008-08-01 00:00:00

  • Brace treatment for osteoarthritis of the knee: a prospective randomized multi-centre trial.

    abstract：OBJECTIVE:To evaluate the effect of a brace intended to reduce load in patients with medial or lateral compartmental osteoarthritis (OA) and concurrent varus or valgus alignment, respectively. DESIGN:This multi-centre randomized controlled trial (performed 2001-2003) studies the additive effect of a brace intended to ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,多中心研究,随机对照试验

    doi：10.1016/j.joca.2006.02.004

    authors： Brouwer RW,van Raaij TM,Verhaar JA,Coene LN,Bierma-Zeinstra SM

    更新日期：2006-08-01 00:00:00

  • The chemokine receptor CCR5 plays a role in post-traumatic cartilage loss in mice, but does not affect synovium and bone.

    abstract：OBJECTIVE:C-C chemokine receptor type 5 (CCR5) has been implicated in rheumatoid arthritis and several inflammatory diseases, where its blockade resulted in reduced joint destruction. However, its role in modulating cartilage and bone changes in post-traumatic osteoarthritis (OA) has not yet been investigated. In this ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2014.12.002

    authors： Takebe K,Rai MF,Schmidt EJ,Sandell LJ

    更新日期：2015-03-01 00:00:00

  • Imaging atlas for eligibility and on-study safety of potential shoulder adverse events in anti-NGF studies (Part 3).

    abstract：:Despite promising results, the U.S. Food and Drug Administration (FDA) put on hold trials assessing anti-nerve growth factor (a-NGF) compounds due to concerns over accelerated rates of OA progression. The mechanism of these events is unclear but joint adverse events were observed particularly in patients using a-NGFs ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2014.09.018

    authors： Roemer FW,Hayes CW,Miller CG,Hoover K,Guermazi A

    更新日期：2015-01-01 00:00:00

  • Measurement properties of performance-based outcome measures to assess physical function in young and middle-aged people known to be at high risk of hip and/or knee osteoarthritis: a systematic review.

    abstract：OBJECTIVES:To systematically appraise the evidence on measurement properties of performance-based outcome measures to assess physical function in young and middle-aged people known to be at high risk of hip and/or knee osteoarthritis (OA). METHODS:Electronic searches were performed in MEDLINE, CINAHL, Scopus and SPORT...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,评审

    doi：10.1016/j.joca.2013.10.021

    authors： Kroman SL,Roos EM,Bennell KL,Hinman RS,Dobson F

    更新日期：2014-01-01 00:00:00

  • Sustained efficacy of a single intra-articular dose of FX006 in a rat model of repeated localized knee arthritis.

    abstract：OBJECTIVE:To evaluate the efficacy of a single intra-articular (IA) dose of FX006, an extended-release formulation of triamcinolone acetonide (TCA) in poly(lactic-co-glycolic acid) (PLGA) microspheres, on the sequelae of repeated episodes of synovitis. DESIGN:Three flares of localized synovitis in the right knee of ra...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2014.09.019

    authors： Kumar A,Bendele AM,Blanks RC,Bodick N

    更新日期：2015-01-01 00:00:00

  • Evaluation of the therapeutic effect of treatment with intra-articular hyaluronic acid in knees for Kashin-Beck disease: a meta-analysis.

    abstract：OBJECTIVE:To assess the efficacy and safety of intra-articular hyaluronic acid (IAHA) injection in knee joints of patients with Kashin-Beck disease (KBD). METHODS:We searched nine electronic databases as well as unpublished data from inception until November 30th 2013 using a combination of search terms for KBD and hy...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,meta分析,评审

    doi：10.1016/j.joca.2014.04.012

    authors： Yu FF,Xia CT,Fang H,Han J,Younus MI,Guo X

    更新日期：2014-06-01 00:00:00

  • Effect of glucosamine sulphate on joint space narrowing, pain and function in patients with hip osteoarthritis; subgroup analyses of a randomized controlled trial.

    abstract：OBJECTIVE:Recently we reported that glucosamine sulphate (GS) did not have an effect on the symptoms and progression of primary care patients with hip osteoarthritis (OA). The aim of this present study was to investigate whether there are subgroups of patients with hip OA for whom GS might be an effective therapy. MET...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.joca.2008.05.022

    authors： Rozendaal RM,Uitterlinden EJ,van Osch GJ,Garling EH,Willemsen SP,Ginai AZ,Verhaar JA,Weinans H,Koes BW,Bierma-Zeinstra SM

    更新日期：2009-04-01 00:00:00

  • The association between reduced knee joint proprioception and medial meniscal abnormalities using MRI in knee osteoarthritis: results from the Amsterdam osteoarthritis cohort.

    abstract：BACKGROUND:Osteoarthritis (OA) of the knee is characterized by pain and activity limitations. In knee OA, proprioceptive accuracy is reduced and might be associated with pain and activity limitations. Although causes of reduced proprioceptive accuracy are divergent, medial meniscal abnormalities, which are highly preva...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2013.02.002

    authors： van der Esch M,Knoop J,Hunter DJ,Klein JP,van der Leeden M,Knol DL,Reiding D,Voorneman RE,Gerritsen M,Roorda LD,Lems WF,Dekker J

    更新日期：2013-05-01 00:00:00

  • Differential induction and regulation of matrix metalloproteinases in osteoarthritic tissue and fluid synovial fibroblasts.

    abstract：OBJECTIVES:To investigate the secretion profiles of matrix metalloproteinases (MMP) and their inhibitors (TIMP) in synovial fluid-derived fibroblasts and to compare them with those of tissue-derived fibroblasts. METHODS:Fibroblast cultures established from synovial tissues (TSC) and fluids (FSC) of the same OA patient...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2004.02.005

    authors： Fuchs S,Skwara A,Bloch M,Dankbar B

    更新日期：2004-05-01 00:00:00

  • Modulation of inflammation by chondroitin sulfate.

    abstract：OBJECTIVE AND METHODS:To evaluate the immune-modulator effect of chondroitin sulfate (CS) by means of the review of the literature. RESULTS:Inflammatory reactions are primarily originated by infectious agents, immune reactions and by sterile tissue lesions that activate membrane receptors by means of pathogen-associat...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,评审

    doi：10.1016/j.joca.2010.02.017

    authors： Vallières M,du Souich P

    更新日期：2010-06-01 00:00:00

  • Osteoarthritis year in review 2017: updates on imaging advancements.

    abstract：OBJECTIVE:This narrative review covers original research publications related to imaging advancements in osteoarthritis (OA) published in the English language between 1st April 2016 and 30th April 2017. METHODS:Relevant human studies (excluding pre-clinical and in vitro studies), were searched and selected from PubMed...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,评审

    doi：10.1016/j.joca.2018.01.007

    authors： Hafezi-Nejad N,Demehri S,Guermazi A,Carrino JA

    更新日期：2018-03-01 00:00:00

  • Optical clearing in collagen- and proteoglycan-rich osteochondral tissues.

    abstract：OBJECTIVE:Recent developments in optical clearing and microscopy technology have enabled the imaging of intact tissues at the millimeter scale to characterize cells via fluorescence labeling. While these techniques have facilitated the three-dimensional (3D) cellular characterization within brain and heart, study of de...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2014.11.021

    authors： Neu CP,Novak T,Gilliland KF,Marshall P,Calve S

    更新日期：2015-03-01 00:00:00

  • Automated segmentation and analysis of normal and osteoarthritic knee menisci from magnetic resonance images--data from the Osteoarthritis Initiative.

    abstract：OBJECTIVE:To validate an automatic scheme for the segmentation and quantitative analysis of the medial meniscus (MM) and lateral meniscus (LM) in magnetic resonance (MR) images of the knee. METHOD:We analysed sagittal water-excited double-echo steady-state MR images of the knee from a subset of the Osteoarthritis Init...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2014.06.029

    authors： Paproki A,Engstrom C,Chandra SS,Neubert A,Fripp J,Crozier S

    更新日期：2014-09-01 00:00:00

  • Tribological rehydration of cartilage and its potential role in preserving joint health.

    abstract：OBJECTIVE:During exercise, cartilage recovers interstitial fluid lost during inactivity, which explains how tissue thickness and joint space are maintained over time. This recovery phenomenon is currently explained by a combination of osmotic swelling during intermittent bath exposure and sub-ambient pressurization dur...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2016.09.018

    authors： Moore AC,Burris DL

    更新日期：2017-01-01 00:00:00

  • Magnetic resonance imaging (MRI) of articular cartilage in knee osteoarthritis (OA): morphological assessment.

    abstract：OBJECTIVE:Magnetic resonance imaging (MRI) is a three-dimensional imaging technique with unparalleled ability to evaluate articular cartilage. This report reviews the current status of morphological assessment of cartilage with quantitative MRI (qMRI), and its relevance for identifying disease status, and monitoring pr...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,评审

    doi：10.1016/j.joca.2006.02.026

    authors： Eckstein F,Cicuttini F,Raynauld JP,Waterton JC,Peterfy C

    更新日期：2006-01-01 00:00:00