Abstract：
OBJECTIVE:To investigate the biosynthetic response of elderly human femoral head articular cartilage to mechanical stimulation in vitro and its variation with site. METHOD:Full-depth cartilage biopsies of articular cartilage were removed from defined sites on 10 femoral heads from patients aged 68-95 years. Cartilage explants were subjected to either static or cyclic (2s on/2s off) loading in unconfined compression at a stress of 1MPa for 24h, or no load. Metabolic activity was assessed by adding medium containing (35)S-sulphate and (3)H-leucine during the last 4h of loading and measuring the incorporated radioisotope. Matrix composition was measured in terms of the amounts of collagen, sulphated glycosaminoglycans (GAG) and water content. RESULTS:Loading of elderly human articular cartilage at 1MPa significantly inhibited incorporation of (35)S-sulphate (P=0.023) into cartilage explants. Pairwise comparisons showed that the difference in incorporation was only for static loading (43% decrease compared to unloaded) (P<0.05). (3)H-leucine incorporation appeared to follow the same trends but neither static nor cyclic load was significantly different from control (P=0.31). Significant topographical variation was found for % GAG wet and GAG:collagen but not water content, % GAG dry or collagen. Isotope incorporation rates were in the order anterior>superior>posterior. CONCLUSION:Static loading inhibits matrix biosynthesis in elderly human cartilage, and cyclic loading is not stimulatory. This is in contrast to previous studies on young bovine tissue where cyclic loading is stimulatory.
journal_name
Osteoarthritis Cartilagejournal_title
Osteoarthritis and cartilageauthors
Plumb MS,Aspden RMdoi
10.1016/j.joca.2005.07.002keywords:
subject
Has Abstractpub_date
2005-12-01 00:00:00pages
1084-91issue
12eissn
1063-4584issn
1522-9653pii
S1063-4584(05)00184-6journal_volume
13pub_type
杂志文章abstract：OBJECTIVES:Femoroacetabular impingement is proposed to cause early osteoarthritis (OA) in the non-dysplastic hip. We previously reported on the prevalence of femoral deformities in a young asymptomatic male population. The aim of this study was to determine the prevalence of both femoral and acetabular types of impinge...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2013.01.003
更新日期：2013-04-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to investigate the ability of full-field optical coherence tomography (FFOCT) to qualitatively and quantitatively evaluate cartilage degeneration using the qualitative evaluation of histology sections as the reference. DESIGN:Thirty-three human knee cartilage samples of variable...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2017.11.008
更新日期：2018-02-01 00:00:00
abstract：OBJECTIVE:The goal of this study was to develop an algorithm to semi-automatically segment the meniscus in a series of magnetic resonance (MR) images to use for normal knees and those with moderate osteoarthritis (OA). METHOD:The segmentation method was developed then evaluated on 10 baseline MR images obtained from s...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2009.10.004
更新日期：2010-03-01 00:00:00
abstract：:Five hundred patients with established, symptomatic limb joint OA have been recruited into an on-going prospective study of the natural history of the condition. Four hundred and fifteen patients (mean age 65.6 years, female to male ratio 2.05:1) were available for a full clinical and radiographic review 3 years after...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/s1063-4584(97)80002-7
更新日期：1997-03-01 00:00:00
abstract：OBJECTIVE:The relative differences in effectiveness of subchondral stimulation, osteochondral grafts, and autologous chondrocyte implantation (ACI) are still unclear. It is the objective of this study to systematically review the literature on ACI compared to other treatments by clinical outcome and the quality of the ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,评审
doi：10.1016/j.joca.2010.03.005
更新日期：2010-06-01 00:00:00
abstract：OBJECTIVE:Chondrocyte death, a notable feature of osteoarthritis, may play a role in the initiation of cartilage degeneration. The present study was aimed at uncovering the nature and involvement of cell death in the initiation of cartilage degeneration induced by mechanical trauma. METHODS:Articular cartilage discs o...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2006.03.012
更新日期：2006-10-01 00:00:00
abstract：OBJECTIVE:The purpose of this paper is to review changes in the biomechanical properties of tendons and ligaments from joint disuse. METHOD:We have reviewed 37 experimental studies on joint disuse, which have been carried out with various models of disuse and with various animals. RESULTS:Immobilization of joints has...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1053/joca.1998.0167
更新日期：1999-01-01 00:00:00
abstract：OBJECTIVE:This study investigated the in vitro effects of hyaluronic acid (HA) of molecular weight (MW) 500-730 kDa on human articular chondrocytes cultivated for 48 h in the presence of interleukin-1beta (IL-1beta) with and without hydrostatic cyclical pressure. DESIGN:The effects of 10 and 100 microg/ml HA with and ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2005.03.006
更新日期：2005-08-01 00:00:00
abstract：:Variations among joints in the initiation and progression of degeneration may be explained, in part, by metabolic, biochemical and biomechanical differences. Compared to the cartilage in the knee joint, ankle cartilage has a higher content of proteoglycans and water, as well as an increased rate of proteoglycan turnov...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,评审
doi：10.1016/j.joca.2004.11.006
更新日期：2005-02-01 00:00:00
abstract：UNLABELLED:Injury to articular cartilage leads to degenerative changes resulting in a loss of mechanical and biochemical properties. In engineered cartilage, the injury response of developing constructs is unclear. OBJECTIVE:To characterize the cellular response of tissue-engineered constructs cultured in chemically-d...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2010.09.003
更新日期：2010-12-01 00:00:00
abstract：OBJECTIVE:This narrative review covers original research publications related to imaging advancements in osteoarthritis (OA) published in the English language between 1st April 2016 and 30th April 2017. METHODS:Relevant human studies (excluding pre-clinical and in vitro studies), were searched and selected from PubMed...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,评审
doi：10.1016/j.joca.2018.01.007
更新日期：2018-03-01 00:00:00
abstract：BACKGROUND:The functional index for hand osteoarthritis (FIHOA), a 10-item investigator-administered questionnaire, was validated in 1995. It is relevant, reliable and has good external and internal validities. OBJECTIVE:To assess the sensitivity to change over time of the FIHOA. PATIENTS/METHODS: PATIENTS:symptomat...
journal_title：Osteoarthritis and cartilage
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1053/joca.2000.0332
更新日期：2000-01-01 00:00:00
abstract：OBJECTIVE:Articular cartilage is separated from subchondral bone by the tidemark and a calcified cartilage zone. Advancement of the calcified region and tidemark duplication are both hallmarks of osteoarthritis (OA). Currently the mechanisms controlling post-natal articular cartilage mineralization are poorly understoo...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2007.05.014
更新日期：2008-01-01 00:00:00
abstract：:The efficacy and the safety of intra-articular injections of sodium hyaluronate were studied in patients with osteoarthritis of the knee in a randomized multicenter double-blind study. Two hundred and nine patients received five injections of either 25 mg hyaluronate/2.5 ml (verum, N = 102) or 0.25 mg hyaluronate/2.5 ...
journal_title：Osteoarthritis and cartilage
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/s1063-4584(05)80329-2
更新日期：1993-10-01 00:00:00
abstract：OBJECTIVE:To quantify the precision and accuracy of measurements of joint space width (JSW) and joint space narrowing (JSN) from the medial tibiofemoral compartment of knee radiographs using a simple and easily adaptable protocol. METHODS:Radiographs of a caliper (a surrogate for JSW) were obtained to determine the pr...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/s1063-4584(03)00158-4
更新日期：2003-10-01 00:00:00
abstract：OBJECTIVE:Subchondral bone attrition (SBA) is defined as flattening or depression of the osseous articular surface. The causes of attrition are unknown, but remodeling processes due to chronic overload that are reflected as bone marrow edema-like lesions (BMLs) on magnetic resonance imaging (MRI) might predispose the s...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,多中心研究
doi：10.1016/j.joca.2009.08.018
更新日期：2010-01-01 00:00:00
abstract：OBJECTIVE:This study is to investigate the functions and underlying mechanisms of mesenchymal stem cells (MSCs) underwent stepwise preconditioning in chondrogenic medium before expansion, then further explore their therapeutic effects in a surgically induced osteoarthritis (OA) model. METHODS:MSCs isolated from the ad...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2017.05.008
更新日期：2017-09-01 00:00:00
abstract：OBJECTIVE:To examine the clinical impact of coexisting lateral osteoarthritis (OA) in knees with mild medial OA. DESIGN:In patients with Kellgren/Lawrence (K/L) grade 2 OA in the medial compartment (n = 100; age: 56-89 years; 80.0% female), anteroposterior knee radiography was used to assess the presence of lateral OA...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2017.02.801
更新日期：2017-08-01 00:00:00
abstract：OBJECTIVE:An age-related decline in chondrocyte production of osteogenic protein-1 (OP-1) (Bone Morphogenetic Protein-7) may contribute to cartilage loss in osteoarthritis. This study was designed to determine if increased methylation of the OP-1 promoter might serve as a mechanism for the age-related decline in OP-1 e...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2008.08.003
更新日期：2009-04-01 00:00:00
abstract：OBJECTIVE:To determine the within- and between-patient variability in the concentrations of synovial fluid, serum and urine markers of joint tissue metabolism in a cohort of patients with knee pain and cartilage changes consistent with early-stage knee osteoarthritis. DESIGN:Samples of synovial fluid, serum, and urine...
journal_title：Osteoarthritis and cartilage
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1053/joca.1998.0134
更新日期：1998-09-01 00:00:00
abstract：OBJECTIVE:To determine whether smoking affects knee cartilage in healthy adults by examining the association of tobacco use with tibial cartilage volume and tibiofemoral cartilage defects. METHODS:Two hundred and ninety-seven healthy adult subjects were recruited from an existing cohort examining healthy aging, the Me...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2006.12.005
更新日期：2007-05-01 00:00:00
abstract：OBJECTIVE:To analyze knee trabecular bone structure and spatial cartilage T(1ρ) and T(2) relaxation times using 3-T magnetic resonance imaging (MRI) in subjects with and without tears of posterior horn of the medial meniscus (PHMM). DESIGN:3-T MRI from 59 subjects (>18 years), were used to evaluate PHMM tears based on...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2012.09.014
更新日期：2013-01-01 00:00:00
abstract：OBJECTIVE:The quantitative interpretation of hip cartilage magnetic resonance imaging (MRI) has been limited by the difficulty of identifying and delineating the cartilage in a three-dimensional (3D) dataset, thereby reducing its routine usage. In this paper a solution is suggested by unfolding the cartilage to planar ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2014.08.012
更新日期：2014-10-01 00:00:00
abstract：OBJECTIVE:Notch signaling has been identified as a critical regulator in cartilage development and joint maintenance, and loss of Notch signaling in all joint tissues results in an early and progressive osteoarthritis (OA)-like pathology. This study investigated the targeted cell population within the knee joint in whi...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2015.10.015
更新日期：2016-04-01 00:00:00
abstract：OBJECTIVE:To evaluate the efficacy and safety of chondroitin sulfate and/or glucosamine hydrochloride in alleviating symptoms and improving the dysfunction of Kashin-Beck disease (KBD) patients. METHODS:We undertook a cluster-randomized, placebo-controlled trial in 251 patients with KBD. Participants were randomly all...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joca.2012.03.013
更新日期：2012-07-01 00:00:00
abstract：OBJECTIVE:Extracellular inorganic pyrophosphate (ePPi) plays a key role in the regulation of normal and pathologic mineralization. The purpose of this work was to evaluate the role of P1 and P2 purine receptors in modulating ePPi production by articular chondrocytes. METHODS:Porcine cartilage explants and chondrocyte ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2010.08.004
更新日期：2010-11-01 00:00:00
abstract：OBJECTIVE:Cam hip shape morphology is a recognised cause of femoroacetabular impingement (FAI) and is associated with hip osteoarthritis. Our aim was to systematically review the available epidemiological evidence assessing the prevalence of cam hip shape morphology in the general population and any studied subgroups i...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,评审
doi：10.1016/j.joca.2015.12.020
更新日期：2016-06-01 00:00:00
abstract：OBJECTIVE:Scottish fold cats, named for their unique ear shape, have a dominantly inherited osteochondrodysplasia involving malformation in the distal forelimbs, distal hindlimbs and tail, and progressive joint destruction. This study aimed to identify the gene and the underlying variant responsible for the osteochondr...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2016.03.019
更新日期：2016-08-01 00:00:00
abstract：OBJECTIVE:To elucidate variations in tissue ultrastructure and incidence of pathology between fibromodulin (FM)-null mice and wild-type (WT) animals. DESIGN:FM-null and WT siblings from different age groups were compared. Serial sections were made through paraffin-embedded whole knees and investigated histologically. ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1053/joca.2002.0527
更新日期：2002-10-01 00:00:00
abstract：OBJECTIVE:To test the efficacy of phase-sensitive X-ray imaging for intact synovial joints, whereby refraction effects, along with the attenuation of conventional radiography, can be exploited. DESIGN:Intact cadaveric human knee joints were imaged, in the computed tomographic mode, using an analyzer-based X-ray system...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2009.03.005
更新日期：2009-09-01 00:00:00
