Abstract:
OBJECTIVE:The hip and knee joints differ biomechanically in terms of contact stresses, fluid lubrication and wear patterns. These differences may be reflected in the synovial fluid (SF) composition of the two joints, but the nature of these differences remains unknown. The objective was to identify differences in osteoarthritic hip and knee SF metabolites using metabolic profiling with Nuclear Magnetic Resonance (NMR) spectroscopy. DESIGN:Twenty-four SF samples (12 hip, 12 knee) were collected from patients with end-stage osteoarthritis (ESOA) undergoing hip/knee arthroplasty. Samples were matched for age, gender, ethnicity and had similar medical comorbidities. NMR spectroscopy was used to analyse the metabolites present in each sample. Principal Component Analysis and Orthogonal Partial Least Squares Discriminant Analysis were undertaken to investigate metabolic differences between the groups. Metabolites were identified using 2D NMR spectra, statistical spectroscopy and by comparison to entries in published databases. RESULTS:There were significant differences in the metabolic profile between the groups. Four metabolites were found in significantly greater quantities in the knee group compared to the hip group (N-acetylated molecules, glycosaminoglycans, citrate and glutamine). CONCLUSIONS:This is the first study to indicate differences in the metabolic profile of hip and knee SF in ESOA. The identified metabolites can broadly be grouped into those involved in collagen degradation, the tricarboxylic acid cycle and oxidative metabolism in diseased joints. These findings may represent a combination of intra and extra-articular factors.
journal_name
Osteoarthritis Cartilagejournal_title
Osteoarthritis and cartilageauthors
Akhbari P,Jaggard MK,Boulangé CL,Vaghela U,Graça G,Bhattacharya R,Lindon JC,Williams HRT,Gupte CMdoi
10.1016/j.joca.2019.07.017subject
Has Abstractpub_date
2019-12-01 00:00:00pages
1768-1777issue
12eissn
1063-4584issn
1522-9653pii
S1063-4584(19)31175-6journal_volume
27pub_type
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journal_title:Osteoarthritis and cartilage
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journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
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journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
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journal_title:Osteoarthritis and cartilage
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更新日期:2018-12-01 00:00:00
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journal_title:Osteoarthritis and cartilage
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更新日期:2008-01-01 00:00:00
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journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2020.01.017
更新日期:2020-06-01 00:00:00
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journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,随机对照试验
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journal_title:Osteoarthritis and cartilage
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pub_type: 杂志文章,随机对照试验
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更新日期:2017-02-01 00:00:00
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journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1053/joca.1998.0141
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journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2014.08.012
更新日期:2014-10-01 00:00:00
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journal_title:Osteoarthritis and cartilage
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doi:10.1016/s1063-4584(05)80055-x
更新日期:1995-09-01 00:00:00
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journal_title:Osteoarthritis and cartilage
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更新日期:2000-05-01 00:00:00
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journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2009.03.001
更新日期:2009-08-01 00:00:00
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journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1053/joca.2001.0509
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更新日期:2003-01-01 00:00:00
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journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2007.03.006
更新日期:2007-09-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2009-11-01 00:00:00
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更新日期:2012-06-01 00:00:00
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journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2005.07.002
更新日期:2005-12-01 00:00:00
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journal_title:Osteoarthritis and cartilage
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doi:10.1016/j.joca.2009.10.009
更新日期:2010-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2018-02-01 00:00:00
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journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
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更新日期:2010-12-01 00:00:00
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journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2016.11.013
更新日期:2017-05-01 00:00:00
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更新日期:2003-06-01 00:00:00