Abstract：
:Multiple sclerosis (MS) is a disease of the CNS in which inflammation, demyelination and neurodegeneration contribute to its initiation and progression. A frequently employed model of MS is experimental autoimmune encephalomyelitis (EAE). Here, to gain new insights into the disease process, an analysis of proteins in extracts of lumbar spinal cord from naïve and EAE rats was undertaken. The data mainly confirm that inflammation and blood-brain barrier (BBB) breakdown are the major hallmarks of disease in this model. Given their importance in the BBB, junctional proteins were further investigated. Occludin, a protein localizing to tight junctions in brain endothelial cells, showed strikingly increased migration in EAE when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). This increased migration was mimicked by in vitro phosphatase treatment, implying its dephosphorylation in EAE. Occludin dephosphorylation coincided with the onset of inflammation, slightly preceding visible signs of disease, and was just prior to apparent changes in BBB permeability. These findings suggest occludin is a target for signaling processes in EAE, perhaps regulating the response of the BBB to the inflammatory environment as seen in MS.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Morgan L,Shah B,Rivers LE,Barden L,Groom AJ,Chung R,Higazi D,Desmond H,Smith T,Staddon JMdoi
10.1016/j.neuroscience.2007.04.051subject
Has Abstractpub_date
2007-07-13 00:00:00pages
664-73issue
3eissn
0306-4522issn
1873-7544pii
S0306-4522(07)00468-Xjournal_volume
147pub_type
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