Expressed sequence tags analysis of a liver tissue cDNA library from a highly inbred minipig line.

Abstract:

BACKGROUND:Porcine liver performing efficient physiological functions in the human body is prerequisite for successful liver xenotransplantation. However, the protein differences between pig and human remain largely unexplored. Therefore, we investigated the liver expression profile of a highly inbred minipig line. METHODS:A cDNA library was constructed from liver tissue of an inbred Banna minipig. Two hundred randomly selected clones were sequenced then analysed by BLAST programme. RESULTS:Alignments of the sequences showed 44% encoded previously known porcine genes. Among the 56% unknown genes, sequences of 72 clones had high similarities with known genes of other species and the similarities to human were mostly above 0.80. The other 40 clones showing no similarity to genes in National Centre for Biotechnology Information are newly discovered, expressed sequence tags specific to liver of inbred Banna minipig. Twenty-two of the 200 clones had full length encoding regions, 38 complete 5' terminal sequences and 140 complete 3' terminal sequences. CONCLUSION:These newly discovered expression sequences may be an important resource for research involving physiological characteristics and medical usage of inbred pigs and contribute to matching studies in xenotransplantation.

journal_name

Chin Med J (Engl)

journal_title

Chinese medical journal

authors

Chen YN,Tan WD,Lu YR,Qin SF,Li SF,Zeng YZ,Bu H,Li YP,Cheng JQ

subject

Has Abstract

pub_date

2007-05-05 00:00:00

pages

739-42

issue

9

eissn

0366-6999

issn

2542-5641

journal_volume

120

pub_type

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