当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Protection of the glutamate pool concentration in enteric bacteria.

Protection of the glutamate pool concentration in enteric bacteria.

Abstract：

:The central nitrogen metabolic circuit in enteric bacteria consists of three enzymes: glutamine synthetase, glutamate synthase (GOGAT), and glutamate dehydrogenase (GDH). With the carbon skeleton provided by 2-oxoglutarate, ammonia/ammonium (NH(4)(+)) is assimilated into two central nitrogen intermediates, glutamate and glutamine. Although both serve as nitrogen donors for all biosynthetic needs, glutamate and glutamine play different roles. Internal glutamine serves as a sensor of external nitrogen availability, and its pool concentration decreases upon nitrogen limitation. A high glutamate pool concentration is required to maintain the internal K(+) pool. The configuration of high glutamate and low glutamine pools was disrupted in GOGAT(-) mutants under low NH(4)(+) conditions: the glutamate pool was low, the difference between glutamate and glutamine was diminished, and growth was defective. When a GOGAT(-) mutant was cultured in an NH(4)(+)-limited chemostat, two sequential spontaneous mutations occurred. Each resulted in a suppressor mutant that outgrew its predecessor in the chemostat. The first suppressor overexpressed GDH, and the second also had a partially impaired glutamine synthetase. The result was a triple mutant in which NH(4)(+) was assimilated by two enzymes instead of the normal three and yet glutamate and glutamine pools and growth were essentially normal. The results indicate preference for the usual ratio of glutamate and glutamine and the resilient and compensatory nature of the circuit on pool control. Analysis of other suppressor mutants selected on solid medium suggests that increased GDH expression is the key for rescue of the growth defect of GOGAT(-) mutants under low NH(4)(+) conditions.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Yan D

doi

10.1073/pnas.0703360104

subject

Has Abstract

pub_date

2007-05-29 00:00:00

pages

9475-80

issue

22

eissn

0027-8424

issn

1091-6490

pii

0703360104

journal_volume

104

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • The antiangiogenic agent TNP-470 requires p53 and p21CIP/WAF for endothelial cell growth arrest.

    abstract：:Targeting the endothelial cell cycle as an antiangiogenic strategy has been difficult given the ubiquitous expression of critical cell cycle regulators. Here, we show that the antiangiogenic drug TNP-470 displays striking cell-type specificity insofar as it induces the expression of p21(CIP/WAF), a cyclin-dependent ki...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.97.23.12782

    authors： Yeh JR,Mohan R,Crews CM

    更新日期：2000-11-07 00:00:00

  • A naturally occurring insertion of a single amino acid rewires transcriptional regulation by glucocorticoid receptor isoforms.

    abstract：:In addition to guiding proteins to defined genomic loci, DNA can act as an allosteric ligand that influences protein structure and activity. Here we compared genome-wide binding, transcriptional regulation, and, using NMR, the conformation of two glucocorticoid receptor (GR) isoforms that differ by a single amino acid...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1316235110

    authors： Thomas-Chollier M,Watson LC,Cooper SB,Pufall MA,Liu JS,Borzym K,Vingron M,Yamamoto KR,Meijsing SH

    更新日期：2013-10-29 00:00:00

  • AKAP220 manages apical actin networks that coordinate aquaporin-2 location and renal water reabsorption.

    abstract：:Filtration through the kidney eliminates toxins, manages electrolyte balance, and controls water homeostasis. Reabsorption of water from the luminal fluid of the nephron occurs through aquaporin-2 (AQP2) water pores in principal cells that line the kidney-collecting duct. This vital process is impeded by formation of ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1607745113

    authors： Whiting JL,Ogier L,Forbush KA,Bucko P,Gopalan J,Seternes OM,Langeberg LK,Scott JD

    更新日期：2016-07-26 00:00:00

  • Crystal structure of an anti-carbohydrate antibody directed against Vibrio cholerae O1 in complex with antigen: molecular basis for serotype specificity.

    abstract：:The crystal structure of the murine Fab S-20-4 from a protective anti-cholera Ab specific for the lipopolysaccharide Ag of the Ogawa serotype has been determined in its unliganded form and in complex with synthetic fragments of the Ogawa O-specific polysaccharide (O-SP). The upstream terminal O-SP monosaccharide is sh...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.060022997

    authors： Villeneuve S,Souchon H,Riottot MM,Mazie JC,Lei P,Glaudemans CP,Kovác P,Fournier JM,Alzari PM

    更新日期：2000-07-18 00:00:00

  • Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia.

    abstract：:Micro-RNAs (miR genes) are a large family of highly conserved noncoding genes thought to be involved in temporal and tissue-specific gene regulation. MiRs are transcribed as short hairpin precursors ( approximately 70 nt) and are processed into active 21- to 22-nt RNAs by Dicer, a ribonuclease that recognizes target m...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.242606799

    authors： Calin GA,Dumitru CD,Shimizu M,Bichi R,Zupo S,Noch E,Aldler H,Rattan S,Keating M,Rai K,Rassenti L,Kipps T,Negrini M,Bullrich F,Croce CM

    更新日期：2002-11-26 00:00:00

  • A shared cis-regulatory module activates transcription in the suspensor of plant embryos.

    abstract：:The mechanisms controlling the transcription of gene sets in specific regions of a plant embryo shortly after fertilization remain unknown. Previously, we showed that G564 mRNA, encoding a protein of unknown function, accumulates to high levels in the giant suspensor of both Scarlet Runner Bean (SRB) and Common Bean e...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1805802115

    authors： Henry KF,Bui AQ,Kawashima T,Goldberg RB

    更新日期：2018-06-19 00:00:00

  • Quality control of assembly-defective U1 snRNAs by decapping and 5'-to-3' exonucleolytic digestion.

    abstract：:The accurate biogenesis of RNA-protein complexes is a key aspect of eukaryotic cells. Defects in Sm protein complex binding to snRNAs are known to reduce levels of snRNAs, suggesting an unknown quality control system for small nuclear ribonucleoprotein (snRNP) assembly. snRNA quality control may also be relevant in sp...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1412614111

    authors： Shukla S,Parker R

    更新日期：2014-08-12 00:00:00

  • Maternal effort mediates the prevalence of trypanosomes in the offspring of a passerine bird.

    abstract：:The relationships between parental effort, offspring growth, and offspring blood parasitemias are poorly known. We examined the effect of parental effort on offspring size and prevalence of trypanosomes in peripheral blood of nestling Pied Flycatchers Ficedula hypoleuca aged 13 days. Trypanosome infections were likely...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.12.5726

    authors： Merino S,Potti J,Moreno J

    更新日期：1996-06-11 00:00:00

  • Inflammation-dependent secretion and splicing of IL-32{gamma} in rheumatoid arthritis.

    abstract：:Different splice variants of the proinflammatory cytokine IL-32 are found in various tissues; their putative differences in biological function remain unknown. In the present study, we report that IL-32γ is the most active isoform of the cytokine. Splicing to one less active IL-32β appears to be a salvage mechanism to...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1016005108

    authors： Heinhuis B,Koenders MI,van de Loo FA,Netea MG,van den Berg WB,Joosten LA

    更新日期：2011-03-22 00:00:00

  • Oil platforms off California are among the most productive marine fish habitats globally.

    abstract：:Secondary (i.e., heterotrophic or animal) production is a main pathway of energy flow through an ecosystem as it makes energy available to consumers, including humans. Its estimation can play a valuable role in the examination of linkages between ecosystem functions and services. We found that oil and gas platforms of...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1411477111

    authors： Claisse JT,Pondella DJ 2nd,Love M,Zahn LA,Williams CM,Williams JP,Bull AS

    更新日期：2014-10-28 00:00:00

  • Ascorbic acid inhibits replication and infectivity of avian RNA tumor virus.

    abstract：:Ascorbic acid, at nontoxic concentrations, causes a substantial reduction in the ability of avian tumor viruses to replicate in both primary avian tendon cells and chicken embryo fibroblasts. The virus-infected cultures appear to be less transformed in the presence of ascorbic acid by the criteria of morphology, reduc...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.77.5.2711

    authors： Bissell MJ,Hatie C,Farson DA,Schwarz RI,Soo WJ

    更新日期：1980-05-01 00:00:00

  • A single polypeptide catalyzing the conversion of phytoene to zeta-carotene is transcriptionally regulated during tomato fruit ripening.

    abstract：:The cDNA of the gene pds from tomato, encoding the carotenoid biosynthesis enzyme phytoene desaturase, was cloned, and its nucleotide sequence was determined. Cells of Escherichia coli that expressed the tomato pds gene could convert phytoene to zeta-carotene. This result suggests that one polypeptide, the product of ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.11.4962

    authors： Pecker I,Chamovitz D,Linden H,Sandmann G,Hirschberg J

    更新日期：1992-06-01 00:00:00

  • Interaction of the yeast Swi4 and Swi6 cell cycle regulatory proteins in vitro.

    abstract：:In budding yeast, two transcription factors, Swi4 and Swi6, control the expression of important cell cycle regulatory proteins (the G1 cyclins, Cln1 and Cln2, and the cyclin-like Hcs26) as well as the HO gene, whose product initiates mating-type switching. Both Swi4 and Swi6 are components of a protein complex that fo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.24.11852

    authors： Andrews BJ,Moore LA

    更新日期：1992-12-15 00:00:00

  • Histone octamer dissociation is not required for transcript elongation through arrays of nucleosome cores by phage T7 RNA polymerase in vitro.

    abstract：:We have examined whether dissociation of the histone octamer is required for elongation of RNA transcripts through arrays of nucleosome cores in vitro. Control or dimethyl suberimidate-crosslinked histone octamers were reconstituted onto supercoiled, closed circular pT207-18 DNA, which contains tandemly repeated 207-b...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.13.6203

    authors： O'Neill TE,Smith JG,Bradbury EM

    更新日期：1993-07-01 00:00:00

  • Candidate tumor suppressor BTG3 maintains genomic stability by promoting Lys63-linked ubiquitination and activation of the checkpoint kinase CHK1.

    abstract：:B-cell translocation gene 3 (BTG3) is a member of the antiproliferative BTG/ Transducer of ErbB2 gene family and is induced by genotoxic stress in a p53- and Checkpoint kinase 1 (CHK1)-dependent manner. Down-regulation of BTG3 has been observed in human cancers, suggesting that it plays an important role in tumor supp...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1220635110

    authors： Cheng YC,Lin TY,Shieh SY

    更新日期：2013-04-09 00:00:00

  • Tocopherol controls D1 amino acid oxidation by oxygen radicals in Photosystem II.

    abstract：:Photosystem II (PSII) is an intrinsic membrane protein complex that functions as a light-driven water:plastoquinone oxidoreductase in oxygenic photosynthesis. Electron transport in PSII is associated with formation of reactive oxygen species (ROS) responsible for oxidative modifications of PSII proteins. In this study...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2019246118

    authors： Kumar A,Prasad A,Sedlářová M,Kale R,Frankel LK,Sallans L,Bricker TM,Pospíšil P

    更新日期：2021-01-26 00:00:00

  • Molecular and structural architecture of polyQ aggregates in yeast.

    abstract：:Huntington's disease is caused by the expansion of a polyglutamine (polyQ) tract in the N-terminal exon of huntingtin (HttEx1), but the cellular mechanisms leading to neurodegeneration remain poorly understood. Here we present in situ structural studies by cryo-electron tomography of an established yeast model system ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1717978115

    authors： Gruber A,Hornburg D,Antonin M,Krahmer N,Collado J,Schaffer M,Zubaite G,Lüchtenborg C,Sachsenheimer T,Brügger B,Mann M,Baumeister W,Hartl FU,Hipp MS,Fernández-Busnadiego R

    更新日期：2018-04-10 00:00:00

  • Amygdala lesions eliminate viewing preferences for faces in rhesus monkeys.

    abstract：:In free-viewing experiments, primates orient preferentially toward faces and face-like stimuli. To investigate the neural basis of this behavior, we measured the spontaneous viewing preferences of monkeys with selective bilateral amygdala lesions. The results revealed that when faces and nonface objects were presented...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1807245115

    authors： Taubert J,Flessert M,Wardle SG,Basile BM,Murphy AP,Murray EA,Ungerleider LG

    更新日期：2018-07-31 00:00:00

  • Structure of canine pulmonary surfactant apoprotein: cDNA and complete amino acid sequence.

    abstract：:The apoproteins of pulmonary surfactant (PSAP) are thought to be critical for normal surfactant function. They bind to surfactant phospholipids and enhance their ability to form surface films in vitro. These acidic glycoproteins have monomeric molecular weights of 36,000, 32,000, and 28,000 (PSAP-36, -32, and -28). Ea...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.19.6379

    authors： Benson B,Hawgood S,Schilling J,Clements J,Damm D,Cordell B,White RT

    更新日期：1985-10-01 00:00:00

  • Y chromosome diversity, human expansion, drift, and cultural evolution.

    abstract：:The relative importance of the roles of adaptation and chance in determining genetic diversity and evolution has received attention in the last 50 years, but our understanding is still incomplete. All statements about the relative effects of evolutionary factors, especially drift, need confirmation by strong demograph...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0910803106

    authors： Chiaroni J,Underhill PA,Cavalli-Sforza LL

    更新日期：2009-12-01 00:00:00

  • Membrane nanotubes induced by aqueous phase separation and stabilized by spontaneous curvature.

    abstract：:Tubular membrane structures are widespread in eukaryotic cells, but the mechanisms underlying their formation and stability are not well understood. Previous work has focused on tube extrusion from cells and model membranes under the application of external forces. Here, we present novel membrane/polymer systems, wher...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1015892108

    authors： Li Y,Lipowsky R,Dimova R

    更新日期：2011-03-22 00:00:00

  • Convergent evolution toward an improved growth rate and a reduced resistance range in Prochlorococcus strains resistant to phage.

    abstract：:Prochlorococcus is an abundant marine cyanobacterium that grows rapidly in the environment and contributes significantly to global primary production. This cyanobacterium coexists with many cyanophages in the oceans, likely aided by resistance to numerous co-occurring phages. Spontaneous resistance occurs frequently i...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1420347112

    authors： Avrani S,Lindell D

    更新日期：2015-04-28 00:00:00

  • Isolation and characterization of a dual-substrate phosphodiesterase gene family: PDE10A.

    abstract：:We report here the cloning, expression, and characterization of a dual-substrate, cAMP and cGMP, cyclic nucleotide phosphodiesterase (PDE) from mouse. This PDE contains the consensus sequence for a PDE catalytic domain, but shares <50% sequence identity with the catalytic domains of all other known PDEs and, therefore...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.12.7071

    authors： Soderling SH,Bayuga SJ,Beavo JA

    更新日期：1999-06-08 00:00:00

  • Epinephrine binding to the catecholamine receptor and activation of the adenylate cyclase in erythrocyte membranes (hormone receptor- -adrenergic receptor-cyclic AMP-turkey).

    abstract：:Turkey erythrocyte membranes showed specific binding of [(3)H]epinephrine. The concentration of hormone required for half-maximal binding (30 muM) was the same as that required for half-maximal activation of the adenylate cyclase located in the same membrane preparation. The binding reaction at 37 degrees C reached co...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.69.2.523

    authors： Schramm M,Feinstein H,Naim E,Lang E,Lasser M

    更新日期：1972-02-01 00:00:00

  • Thermal motions and function of bacteriorhodopsin in purple membranes: effects of temperature and hydration studied by neutron scattering.

    abstract：:The internal dynamics of bacteriorhodopsin, the light-driven proton pump in the purple membrane of Halobacterium halobium, has been studied by inelastic neutron scattering for various conditions of temperature and hydration. Light activation can take place when the membrane is vibrating harmonically. The ability of th...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.20.9668

    authors： Ferrand M,Dianoux AJ,Petry W,Zaccaï G

    更新日期：1993-10-15 00:00:00

  • Attenuation of focal cerebral ischemic injury in transgenic mice overexpressing CuZn superoxide dismutase.

    abstract：:Oxygen-derived free radicals have been implicated in the pathogenesis of vasogenic edema and infarction caused by ischemia and reperfusion injury. In earlier studies, exogenously supplied liposome-entrapped CuZn superoxide dismutase (CuZn-SOD) ameliorated ischemic brain edema and infarction in rats following focal cer...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.24.11158

    authors： Kinouchi H,Epstein CJ,Mizui T,Carlson E,Chen SF,Chan PH

    更新日期：1991-12-15 00:00:00

  • DNA hybridization evidence for the Australasian affinity of the American marsupial Dromiciops australis.

    abstract：:DNA hybridization was used to compare representatives of the major groups of marsupials and a eutherian outgroup. Because of the large genetic distances separating marsupial families, trees were calculated from normalized percentages of hybridization; thermal-melting statistics, however, gave identical topologies for ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.23.10465

    authors： Kirsch JA,Dickerman AW,Reig OA,Springer MS

    更新日期：1991-12-01 00:00:00

  • Sulfur radical species form gold deposits on Earth.

    abstract：:Current models of the formation and distribution of gold deposits on Earth are based on the long-standing paradigm that hydrogen sulfide and chloride are the ligands responsible for gold mobilization and precipitation by fluids across the lithosphere. Here we challenge this view by demonstrating, using in situ X-ray a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1506378112

    authors： Pokrovski GS,Kokh MA,Guillaume D,Borisova AY,Gisquet P,Hazemann JL,Lahera E,Del Net W,Proux O,Testemale D,Haigis V,Jonchière R,Seitsonen AP,Ferlat G,Vuilleumier R,Saitta AM,Boiron MC,Dubessy J

    更新日期：2015-11-03 00:00:00

  • Natural and synthetic prion structure from X-ray fiber diffraction.

    abstract：:A conformational isoform of the mammalian prion protein (PrP(Sc)) is the sole component of the infectious pathogen that causes the prion diseases. We have obtained X-ray fiber diffraction patterns from infectious prions that show cross-beta diffraction: meridional intensity at 4.8 A resolution, indicating the presence...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0909006106

    authors： Wille H,Bian W,McDonald M,Kendall A,Colby DW,Bloch L,Ollesch J,Borovinskiy AL,Cohen FE,Prusiner SB,Stubbs G

    更新日期：2009-10-06 00:00:00

  • Caspase cleavage of MST1 promotes nuclear translocation and chromatin condensation.

    abstract：:MST1, mammalian STE20-like kinase 1, is a serine/threonine kinase that is cleaved and activated by caspases during apoptosis. MST1 is capable of inducing apoptotic morphological changes such as chromatin condensation upon overexpression. In this study, we show that MST1 contains two functional nuclear export signals (...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.181161698

    authors： Ura S,Masuyama N,Graves JD,Gotoh Y

    更新日期：2001-08-28 00:00:00