当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Caspase cleavage of MST1 promotes nuclear translocation and chromatin condensation.

Caspase cleavage of MST1 promotes nuclear translocation and chromatin condensation.

Abstract：

:MST1, mammalian STE20-like kinase 1, is a serine/threonine kinase that is cleaved and activated by caspases during apoptosis. MST1 is capable of inducing apoptotic morphological changes such as chromatin condensation upon overexpression. In this study, we show that MST1 contains two functional nuclear export signals (NESs) in the C-terminal domain, which is released from the N-terminal kinase domain upon caspase-mediated cleavage. Full-length MST1 is excluded from the nucleus and localized to the cytoplasm. However, either truncation of the C-terminal domain, point mutation of the two putative NESs, or treatment with leptomycin B, an inhibitor of the NES receptor, results in nuclear localization of MST1. Staurosporine treatment induces chromatin condensation, MST1 cleavage, and nuclear translocation. Staurosporine-induced chromatin condensation is partially inhibited by expressing a kinase-negative mutant of MST1, suggesting an important role of MST1 in this process. Significantly, MST1 is more efficient at inducing chromatin condensation when it is constitutively localized to the nucleus by mutation of its NESs. Moreover, inhibition of MST1 nuclear translocation by mutation of its cleavage sites reduces its ability to induce chromatin condensation. Taken together, these results suggest that truncation of the C-terminal domain of MST1 by caspases may result in translocation of MST1 into the nucleus, where it promotes chromatin condensation.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Ura S,Masuyama N,Graves JD,Gotoh Y

doi

10.1073/pnas.181161698

keywords:

subject

Has Abstract

pub_date

2001-08-28 00:00:00

pages

10148-53

issue

18

eissn

0027-8424

issn

1091-6490

pii

181161698

journal_volume

98

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Predicting the evolutionary dynamics of seasonal adaptation to novel climates in Arabidopsis thaliana.

    abstract：:Predicting whether and how populations will adapt to rapid climate change is a critical goal for evolutionary biology. To examine the genetic basis of fitness and predict adaptive evolution in novel climates with seasonal variation, we grew a diverse panel of the annual plant Arabidopsis thaliana (multiparent advanced...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 评论,杂志文章

    doi：10.1073/pnas.1517456113

    authors： Fournier-Level A,Perry EO,Wang JA,Braun PT,Migneault A,Cooper MD,Metcalf CJ,Schmitt J

    更新日期：2016-05-17 00:00:00

  • Concerted biosynthesis of an insect elicitor of plant volatiles.

    abstract：:A variety of agricultural plant species, including corn, respond to insect herbivore damage by releasing large quantities of volatile compounds and, as a result, become highly attractive to parasitic wasps that attack the herbivores. An elicitor of plant volatiles, N-(17-hydroxylinolenoyl)-L-glutamine, named volicitin...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.23.13971

    authors： Paré PW,Alborn HT,Tumlinson JH

    更新日期：1998-11-10 00:00:00

  • Functional identification of the hematopoietic stem cell niche in the ventral domain of the embryonic dorsal aorta.

    abstract：:The first definitive/adult-type hematopoietic stem cells (HSCs) in the mouse aorta-gonad-mesonephros region emerge between embryonic days 10.5 and 11.5. The discovery of clusters of hematopoietic cells on the ventral luminal surface of the dorsal aorta in various vertebrate species has led to speculation that the floo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0700984104

    authors： Taoudi S,Medvinsky A

    更新日期：2007-05-29 00:00:00

  • Fluorescence polarization and viscosities of membrane lipids of 3T3 cells.

    abstract：:Studies of the fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene in membranes of normal, transformed, and revertant 3T3 cells allowed estimation of microviscosities (eta) of the lipid bilayers of these cells. Fluorescence polarization values observed with normal cells were significantly lower than those obser...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.72.9.3351

    authors： Fuchs P,Parola A,Robbins PW,Blout ER

    更新日期：1975-09-01 00:00:00

  • Cell-free translation of highly purified adenovirus messenger RNA.

    abstract：:Several polypeptides contained in the coat and the core of adenovirus type 2 particles have been identified as virus gene products. They are synthesized in vitro by a cell-free protein-making system programmed by adenovirus type 2 messenger RNA that has been hybridized with and eluted from virus DNA. Fractionation by ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.71.9.3385

    authors： Eron L,Westphal H

    更新日期：1974-09-01 00:00:00

  • Atherosclerosis and hypertension induction by lead and cadmium ions: an effect prevented by calcium ion.

    abstract：:In epidemiological studies, both positive and negative correlations have been found between cardiovascular disease and mortality and the presence of several inorganic ions in the drinking water. In an attempt to resolve this apparent disagreement, we exposed White Carneau pigeons to drinking water containing calcium (...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.10.6494

    authors： Revis NW,Zinsmeister AR,Bull R

    更新日期：1981-10-01 00:00:00

  • In vivo and in vitro observations on laminin production by Schwann cells.

    abstract：:Recent reports on the dystrophic mouse mutant suggest that the prominent extracellular matrix component of peripheral nerve tissues plays an important role in peripheral nerve development. We have examined the disposition of two prominent extracellular matrix components, fibronectin and laminin, both in mature periphe...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.80.12.3850

    authors： Cornbrooks CJ,Carey DJ,McDonald JA,Timpl R,Bunge RP

    更新日期：1983-06-01 00:00:00

  • Progressive forest canopy water loss during the 2012-2015 California drought.

    abstract：:The 2012-2015 drought has left California with severely reduced snowpack, soil moisture, ground water, and reservoir stocks, but the impact of this estimated millennial-scale event on forest health is unknown. We used airborne laser-guided spectroscopy and satellite-based models to assess losses in canopy water conten...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1523397113

    authors： Asner GP,Brodrick PG,Anderson CB,Vaughn N,Knapp DE,Martin RE

    更新日期：2016-01-12 00:00:00

  • Uridine diphosphate N-acetylglucosamine stimulates uptake of nutrients by quiescent BALB/C3T3 cells.

    abstract：:Preincubation of quiescent BALB/C3T3 cells with uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) in conditioned medium resulted in a time- and concentration-dependent stimulation of uptake of 2-deoxyglucose, uridine, and alpha-aminoisobutyric acid over that seen in conditioned medium alone. The apparent Km values ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.75.8.3859

    authors： Natraj CV,Datta P

    更新日期：1978-08-01 00:00:00

  • Resting state of the human proton channel dimer in a lipid bilayer.

    abstract：:The voltage-gated proton channel Hv1 plays a critical role in the fast proton translocation that underlies a wide range of physiological functions, including the phagocytic respiratory burst, sperm motility, apoptosis, and metastatic cancer. Both voltage activation and proton conduction are carried out by a voltage-se...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1515043112

    authors： Li Q,Shen R,Treger JS,Wanderling SS,Milewski W,Siwowska K,Bezanilla F,Perozo E

    更新日期：2015-11-03 00:00:00

  • Glucose-regulated interaction of a regulatory subunit of protein phosphatase 1 with the Snf1 protein kinase in Saccharomyces cerevisiae.

    abstract：:The Snf1 protein kinase family has been conserved in eukaryotes. In the yeast Saccharomyces cerevisiae, Snf1 is essential for transcription of glucose-repressed genes in response to glucose starvation. The direct interaction between Snf1 and its activating subunit, Snf4, within the kinase complex is regulated by the g...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.11.6245

    authors： Ludin K,Jiang R,Carlson M

    更新日期：1998-05-26 00:00:00

  • gamma-Aminobutyric acid acts at axon terminals of turtle photoreceptors: difference in sensitivity among cell types.

    abstract：:It has been proposed that horizontal cells of the vertebrate retina have a negative feedback synapse with cone photoreceptors. gamma-Aminobutyric acid (GABA) has been suggested to be a neurotransmitter of monophasic horizontal cells (a subtype of horizontal cells), which have direct connections with red-sensitive and ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.81.24.7961

    authors： Tachibana M,Kaneko A

    更新日期：1984-12-01 00:00:00

  • Novel adeno-associated viruses from rhesus monkeys as vectors for human gene therapy.

    abstract：:Tissues from rhesus monkeys were screened by PCR for the presence of sequences homologous to known adeno-associated virus (AAV) serotypes 1-6. DNA spanning entire rep-cap ORFs from two novel AAVs, called AAV7 and AAV8, were isolated. Sequence comparisons among these and previously described AAVs revealed the greatest ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.182412299

    authors： Gao GP,Alvira MR,Wang L,Calcedo R,Johnston J,Wilson JM

    更新日期：2002-09-03 00:00:00

  • Housing growth in and near United States protected areas limits their conservation value.

    abstract：:Protected areas are crucial for biodiversity conservation because they provide safe havens for species threatened by land-use change and resulting habitat loss. However, protected areas are only effective when they stop habitat loss within their boundaries, and are connected via corridors to other wild areas. The effe...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0911131107

    authors： Radeloff VC,Stewart SI,Hawbaker TJ,Gimmi U,Pidgeon AM,Flather CH,Hammer RB,Helmers DP

    更新日期：2010-01-12 00:00:00

  • The criminogenic and psychological effects of police stops on adolescent black and Latino boys.

    abstract：:Proactive policing, the strategic targeting of people or places to prevent crimes, is a well-studied tactic that is ubiquitous in modern law enforcement. A 2017 National Academies of Sciences report reviewed existing literature, entrenched in deterrence theory, and found evidence that proactive policing strategies can...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1808976116

    authors： Del Toro J,Lloyd T,Buchanan KS,Robins SJ,Bencharit LZ,Smiedt MG,Reddy KS,Pouget ER,Kerrison EM,Goff PA

    更新日期：2019-04-23 00:00:00

  • Thymic output in aged mice.

    abstract：:Using GFP to mark recent thymic emigrants (RTEs) in mice carrying a GFP transgene driven by the recombination-activating gene 2 promoter, we demonstrate that RTEs are readily detectable even in 2-year-old mice, despite the fact that the proportion of the peripheral T cell pool comprised of RTEs declines with age. Alth...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0601040103

    authors： Hale JS,Boursalian TE,Turk GL,Fink PJ

    更新日期：2006-05-30 00:00:00

  • Targeted mutagenesis of the murine transferrin receptor-2 gene produces hemochromatosis.

    abstract：:Hereditary hemochromatosis (HH) is a common genetic disorder characterized by excess absorption of dietary iron and progressive iron deposition in several tissues, particularly liver. The vast majority of individuals with HH are homozygous for mutations in the HFE gene. Recently a second transferrin receptor (TFR2) wa...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.162360699

    authors： Fleming RE,Ahmann JR,Migas MC,Waheed A,Koeffler HP,Kawabata H,Britton RS,Bacon BR,Sly WS

    更新日期：2002-08-06 00:00:00

  • Theoretical considerations on the "spine of hydration" in the minor groove of d(CGCGAATTCGCG).d(GCGCTTAAGCGC): Monte Carlo computer simulation.

    abstract：:A theoretical description of aqueous hydration in the minor groove of a B-form DNA is presented on the basis of a liquid-state Monte Carlo computer simulation on a system consisting of the oligonucleotide duplex d(CGCGAATTCGCG).d(GCGCTTAAGCGC) in a canonical B-form together with 1777 water molecules contained in a hex...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.6.1836

    authors： Subramanian PS,Ravishanker G,Beveridge DL

    更新日期：1988-03-01 00:00:00

  • Correlation of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine neurotoxicity with blood-brain barrier monoamine oxidase activity.

    abstract：:Systemic administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) causes parkinsonism in humans and subhuman primates, but not in rats and many other laboratory animals; mice are intermediate in their susceptibility. Since MPTP causes selective dopaminergic neurotoxicity when infused directly into rat sub...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.10.3521

    authors： Kalaria RN,Mitchell MJ,Harik SI

    更新日期：1987-05-01 00:00:00

  • Analysis of a peptide hormone-receptor interaction in the yeast two-hybrid system.

    abstract：:Interaction between a peptide hormone and extracellular domains of its receptor is a crucial step for initiation of hormone action. We have developed a modification of the yeast two-hybrid system to study this interaction and have used it to characterize the interaction of insulin-like growth factor 1 (IGF-1) with its...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.24.13063

    authors： Zhu J,Kahn CR

    更新日期：1997-11-25 00:00:00

  • Alzheimer beta/A4 amyloid precursor protein in human brain: aging-associated increases in holoprotein and in a proteolytic fragment.

    abstract：:Alzheimer beta/A4 amyloid precursor protein (APP) has been suggested to play a central role in the pathogenesis of Alzheimer disease. We have measured the content of different species of APP holoprotein and carboxyl-terminal fragments in human brains from young individuals, nondemented aged individuals, and aged indiv...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.20.8910

    authors： Nordstedt C,Gandy SE,Alafuzoff I,Caporaso GL,Iverfeldt K,Grebb JA,Winblad B,Greengard P

    更新日期：1991-10-15 00:00:00

  • Reactivation of Epstein-Barr virus by a dual-responsive fluorescent EBNA1-targeting agent with Zn2+-chelating function.

    abstract：:Epstein-Barr nuclear antigen 1 (EBNA1) plays a vital role in the maintenance of the viral genome and is the only viral protein expressed in nearly all forms of Epstein-Barr virus (EBV) latency and EBV-associated diseases, including numerous cancer types. To our knowledge, no specific agent against EBV genes or protein...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1915372116

    authors： Jiang L,Lung HL,Huang T,Lan R,Zha S,Chan LS,Thor W,Tsoi TH,Chau HF,Boreström C,Cobb SL,Tsao SW,Bian ZX,Law GL,Wong WT,Tai WC,Chau WY,Du Y,Tang LHX,Chiang AKS,Middeldorp JM,Lo KW,Mak NK,Long NJ,Wong KL

    更新日期：2019-12-10 00:00:00

  • CD14-expressing cancer cells establish the inflammatory and proliferative tumor microenvironment in bladder cancer.

    abstract：:Nonresolving chronic inflammation at the neoplastic site is consistently associated with promoting tumor progression and poor patient outcomes. However, many aspects behind the mechanisms that establish this tumor-promoting inflammatory microenvironment remain undefined. Using bladder cancer (BC) as a model, we found ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1424795112

    authors： Cheah MT,Chen JY,Sahoo D,Contreras-Trujillo H,Volkmer AK,Scheeren FA,Volkmer JP,Weissman IL

    更新日期：2015-04-14 00:00:00

  • Positionally cloned human disease genes: patterns of evolutionary conservation and functional motifs.

    abstract：:Positional cloning has already produced the sequences of more than 70 human genes associated with specific diseases. In addition to their medical importance, these genes are of interest as a set of human genes isolated solely on the basis of the phenotypic effect of the respective mutations. We analyzed the protein se...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.11.5831

    authors： Mushegian AR,Bassett DE Jr,Boguski MS,Bork P,Koonin EV

    更新日期：1997-05-27 00:00:00

  • Nickel pincer model of the active site of lactate racemase involves ligand participation in hydride transfer.

    abstract：:Lactate racemase is the first enzyme known to possess a metal pincer active site. The enzyme interconverts d- and l-lactic acid, which is important for the assembly of cell walls in many microorganisms. Here, we report a synthetic model of the active site of lactate racemase, which features a pyridinium-based SCS pinc...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1616038114

    authors： Xu T,Wodrich MD,Scopelliti R,Corminboeuf C,Hu X

    更新日期：2017-02-07 00:00:00

  • Organ boundary1 defines a gene expressed at the junction between the shoot apical meristem and lateral organs.

    abstract：:We identify a gene, organ boundary1 (OBO1), by its unique pattern of enhancer- driven GFP expression at the boundaries between the apical meristems and lateral organs in Arabidopsis embryos, seedlings, and mature plants. OBO1 also is expressed at the root apical meristem and in distinct cell files surrounding this are...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1018542108

    authors： Cho E,Zambryski PC

    更新日期：2011-02-01 00:00:00

  • Molecular mechanism of water reorientational slowing down in concentrated ionic solutions.

    abstract：:Water dynamics in concentrated ionic solutions plays an important role in a number of material and energy conversion processes such as the charge transfer at the electrolyte-electrode interface in aqueous rechargeable ion batteries. One long-standing puzzle is that all electrolytes, regardless of their "structure-maki...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1707453114

    authors： Zhang Q,Wu T,Chen C,Mukamel S,Zhuang W

    更新日期：2017-09-19 00:00:00

  • Conformational properties of the acetylcholine receptor as revealed by studies with constrained depolarizing ligands.

    abstract：:Conformational aspects of the acetylcholine receptor (AcChoR) of Electrophorus electricus have been examined by studies of its interaction with structurally related, constrained aromatic bis quaternary compounds. Among the compounds synthesized was 3,3'-bis[alpha-(trimethylammonium)-methyl]azobenzene dibromide (3,3'-b...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.76.1.256

    authors： Wassermann NH,Bartels E,Erlanger BF

    更新日期：1979-01-01 00:00:00

  • Negative selection on human genes underlying inborn errors depends on disease outcome and both the mode and mechanism of inheritance.

    abstract：:Genetic variants underlying life-threatening diseases, being unlikely to be transmitted to the next generation, are gradually and selectively eliminated from the population through negative selection. We study the determinants of this evolutionary process in human genes underlying monogenic diseases by comparing vario...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2001248118

    authors： Rapaport F,Boisson B,Gregor A,Béziat V,Boisson-Dupuis S,Bustamante J,Jouanguy E,Puel A,Rosain J,Zhang Q,Zhang SY,Gleeson JG,Quintana-Murci L,Casanova JL,Abel L,Patin E

    更新日期：2021-01-19 00:00:00

  • An adhesion protein of Salmonella enterica serovar Typhi is required for pathogenesis and potential target for vaccine development.

    abstract：:More than half of all Salmonella enterica serovar Typhi genes still remain unannotated. Although pathogenesis of S. Typhi is incompletely understood, treatment of typhoid fever is complicated by the emergence of drug resistance. Effectiveness of the currently available vaccines is also limited. In search of novel viru...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1016180108

    authors： Ghosh S,Chakraborty K,Nagaraja T,Basak S,Koley H,Dutta S,Mitra U,Das S

    更新日期：2011-02-22 00:00:00