Abstract:
BACKGROUND:Intestinal epithelial cell apoptosis has been reported in sepsis as a mechanism of organ failure. The aim of this study was to clarify the role of apoptosis-regulating proteins (bcl-2, bax, cytochrome-c, and caspase-8) in septic rats by studying their expression in gastric and intestinal epithelial cells. METHODS:Adult Wistar rats were subjected to the cecal ligation and puncture (CLP) model of sepsis and randomly divided into two study groups. Sixty-two animals were sacrificed 6, 12, 24, 36, 48, and 60 h post-procedure, and 50 animals served as the survival study group. Sham-operated animals (n = 40) were used as controls. Gastric and intestinal tissue was excised, and immunohistochemical detection of bcl-2, bax, cytochrome-c, and caspase-8 protein expression was performed. RESULTS:In gastric mucosa, sepsis induced upregulation of bax and downregulation of caspase-8 expression (p = 0.053 and p = 0.05, respectively). Both bax and caspase-8 were upregulated as early as 6 h post CLP and progressively decreased (p = 0.001, p = 0.004 respectively). In contrast, the expression of the anti-apoptotic bcl-2 was upregulated progressively during the sepsis syndrome (p = 0.03). In intestine, sepsis induced a fourfold upregulation of the cytoprotective bcl-2 (p = 0.0001), accompanied by a remarkable increase in bax (p = 0.002) and caspase-8 (p = 0.0001) expression and a decrease in cytochrome-c expression (p = 0.02). The time distribution of the apoptosis regulators followed the same pattern as in gastric tissue, showing an upregulation of the proapoptotic bax and cytochrome c (p = 0.04) during the early phases and a progressively increased expression of bcl-2 during the late phases (p = 0.0001). Bax expression in gastric epithelium of subjects with septic syndrome was detrimental to survival (p = 0.0001), whereas the expression of the cytoprotective bcl-2 in intestinal epithelium appeared to favor a good prognosis (p = 0.0001). CONCLUSIONS:Sepsis results in alterations of apoptosis regulators in gastrointestinal cells. Alterations of bax and bcl-2 expression in gastric and intestinal epithelial cells may predict the outcome in septic rats.
journal_name
World J Surgjournal_title
World journal of surgeryauthors
Messaris E,Kekis P,Memos N,Chatzigianni E,Menenakos E,Leandros E,Konstadoulakis MMdoi
10.1007/s00268-005-0742-1subject
Has Abstractpub_date
2007-04-01 00:00:00pages
787-94issue
4eissn
0364-2313issn
1432-2323journal_volume
31pub_type
杂志文章abstract:INTRODUCTION:This study assesses the incidence of missed pelvic injuries in the pre-hospital setting. METHODS:All blunt trauma patients (ISS ≥ 9) with pre-hospital suspicion of and/or radiologically proven pelvic fracture documented in the TraumaRegister DGU® (TR-DGU) of the German Trauma Society DGU (2002-2011) were ...
journal_title:World journal of surgery
pub_type: 杂志文章,多中心研究
doi:10.1007/s00268-016-3647-2
更新日期:2016-12-01 00:00:00
abstract:BACKGROUND:The World Health Organization Surgical Safety Checklist (SSC) has been widely implemented in an effort to decrease surgical adverse events. METHOD:This systematic literature review examined the effects of the SSC on postoperative outcomes. The review included 25 studies: two randomised controlled trials, 13...
journal_title:World journal of surgery
pub_type: 杂志文章,评审
doi:10.1007/s00268-016-3519-9
更新日期:2016-08-01 00:00:00
abstract::Bacterial biofilms develop on a number of living and inert surfaces within the urinary tract, producing chronic intractable infections. The role of exopolysaccharides in producing a variety of extracellular polymeric substances according to the infection site and bacterial species and the clinical features of the resu...
journal_title:World journal of surgery
pub_type: 杂志文章,评审
doi:10.1007/s002680010203
更新日期:2000-10-01 00:00:00
abstract:BACKGROUND:Laparoscopic proximal gastrectomy (LPG) is performed as a function-preserving surgery for patients with early proximal gastric malignant tumors; however, whether LPG has advantages postoperatively compared with laparoscopic total gastrectomy (LTG) is debatable, especially with regard to nutritional outcomes....
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-018-4625-7
更新日期:2018-10-01 00:00:00
abstract:BACKGROUND:In uremic patients, metabolism of 1-84 parathyroid hormone (PTH) and fragments are delayed, and in these patients, the usefulness of intraoperative PTH assay may be problematic. We evaluated the usefulness of the QuiCk-IntraOperative Bio-Intact PTH (QPTH) assay for uremic patients with secondary hyperparathy...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-006-0601-8
更新日期:2007-04-01 00:00:00
abstract:BACKGROUND:Flow disruptions (FDs) are deviations from the progression of care that compromise safety or efficiency. The frequency and specific causes of FDs remain poorly documented in trauma care. We undertook this study to identify and quantify the rate of FDs during various phases of trauma care. METHODS:Seven trai...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-013-2306-0
更新日期:2014-02-01 00:00:00
abstract::With limited resources and the current concerns about using animals for research purposes, the needs must be clear when setting up trauma and sepsis experiments for pharmacologic interventions. Such interventions are performed typically for four reasons: (1) to study the pathophysiologic role of certain mediators (whi...
journal_title:World journal of surgery
pub_type: 杂志文章,评审
doi:10.1007/s002689900077
更新日期:1996-05-01 00:00:00
abstract::The role of nitric oxide in mediating smooth muscle relaxation is well established. Less is known about the downstream signaling events inside the smooth muscle cell that produce relaxation of the contractile machinery. One possible effector molecule is the small heat shock protein HSP20. Increased phosphorylation of ...
journal_title:World journal of surgery
pub_type: 杂志文章,评审
doi:10.1007/s00268-002-4052-6
更新日期:2002-07-01 00:00:00
abstract:OBJECTIVES:We set out to clarify the clinicopathologic characteristics of colorectal liver metastases with macroscopic intrabiliary tumor growth and to determine optimal surgical management. METHODS:Over 15 years, 6 of 103 patients undergoing hepatectomy for colorectal liver metastases had macroscopic intrabiliary tum...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-006-0205-3
更新日期:2006-10-01 00:00:00
abstract:BACKGROUND:Gastroesophageal reflux is the main risk factor for esophageal adenocarcinoma, but there is no strong support for a protective effect of antireflux surgery. We tested the hypothesis that esophageal adenocarcinoma that develops with long latency after antireflux surgery might be due to persistent postoperativ...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-006-0386-9
更新日期:2007-03-01 00:00:00
abstract::This study investigated whether cytokines and colony-stimulating factors can predict prognosis in patients with postoperative multiple organ failure (MOF). We evaluated 14 patients with postoperative MOF who underwent operation for cardiovascular disease. Seven patients recovered from MOF (survivors) and seven did not...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s002689900520
更新日期:1998-10-01 00:00:00
abstract::This study was designed to investigate the optimal dose of garlic during long-term feeding and its preventive and therapeutic effects on colon cancer in rats induced by 1,2-dimethylhydrazine (DMH). A total of 240 male Sprague-Dawley rats were grouped and fed with either a basal or a garlic diet of different concentrat...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/BF00294738
更新日期:1995-07-01 00:00:00
abstract:BACKGROUND:A majority of patients with small intestinal neuroendocrine tumors (SI-NETs) present with or develop liver metastases (LM). A number of treatments for LM are used clinically, including liver transplantation (LTx). Indications for LTx are under debate; young age(<65 years), absence of extrahepatic disease, re...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-013-2331-z
更新日期:2014-03-01 00:00:00
abstract:BACKGROUND:The aim of this study was to compare the results of percutaneous radiofrequency ablation (RFA) with those of partial hepatectomy (PH) in the treatment of multicentric small hepatocellular carcinomas (HCCs). With advances in RFA, it is not known whether the minimally invasive approach with percutaneous RFA co...
journal_title:World journal of surgery
pub_type: 临床试验,杂志文章
doi:10.1007/s00268-010-0732-9
更新日期:2010-11-01 00:00:00
abstract::The development of laparoscopic surgery has provided minimally invasive surgeons with advanced laparoscopic instrumentation and high definition imaging. The resulting surgical expertise and technology has now been extended to gastric endoluminal surgery. Laboratory and clinical investigations have been initiated for v...
journal_title:World journal of surgery
pub_type: 杂志文章,评审
doi:10.1007/pl00012311
更新日期:1999-04-01 00:00:00
abstract:BACKGROUND:Surgical 'never events' have serious adverse outcomes for patients. A never event can be defined as a serious, avoidable patient safety incident that would not occur if necessary preventative measures are implemented. The literature from South Africa on this topic is limited. This study aims to understand th...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-019-05008-9
更新日期:2019-09-01 00:00:00
abstract:BACKGROUND:Pancreatic cancer has higher concentrations of angiotensin II compared with other cancers. This study sought to assess the effect of angiotensin II receptor blockers (ARBs) on survival of patients undergoing resection using a large, nationally representative dataset. METHODS:Patients undergoing pancreatic c...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-017-4021-8
更新日期:2017-09-01 00:00:00
abstract:BACKGROUND:The purpose of this study was to assess the safety and feasibility of performing robot-assisted pediatric surgery using the da Vinci Surgical System in a variety of surgical procedures. METHODS:A retrospective review of 144 robot-assisted pediatric surgical procedures performed in our institution between Ju...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-010-0431-6
更新日期:2010-05-01 00:00:00
abstract:BACKGROUND:The aim of this study was to compare the perioperative outcomes when using a micropuncture access set (MS) to those when using a conventional puncture set (CS) for implantation of totally implantable venous access device (TAVID). METHODS:A total of 314 patients undergoing chemotherapy for colorectal cancer ...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-019-05336-w
更新日期:2020-04-01 00:00:00
abstract:BACKGROUND:This study is intended to ascertain if outcome of acute abdominal surgery among elderly patients with acute abdominal pain have improved. METHODS:Altogether 456 patients aged >65 years underwent emergency abdominal surgery between the years 2007 and 2009 in our hospital. After excluding emergency reoperatio...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-015-3207-1
更新日期:2015-12-01 00:00:00
abstract:BACKGROUND:Acute colonic diverticulitis is a common clinical condition. Severity of the disease is based on clinical, laboratory, and radiological investigations and dictates the need for medical or surgical intervention. Recent clinical trials have improved the understanding of the natural history of the disease resul...
journal_title:World journal of surgery
pub_type: 杂志文章,评审
doi:10.1007/s00268-016-3560-8
更新日期:2016-10-01 00:00:00
abstract:BACKGROUND:Inguinal hernia repair is the most common elective procedure in general surgery. Therefore, the number of patients having complications related to inguinal hernia surgery is relatively large. The aim of this study was to compare complication profiles of inguinal open mesh (OM) hernioplasties with open non-me...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-015-3028-2
更新日期:2015-08-01 00:00:00
abstract:BACKGROUND:The aim of this study was to evaluate and investigate the complications of carotid endarterectomy (CEA) and carotid artery stenting (CAS) by performing a meta-analysis based on prospective randomized controlled trials (RCTs). METHODS:We performed a search of multiple electronic databases for RCTs containing...
journal_title:World journal of surgery
pub_type: 杂志文章,meta分析,评审
doi:10.1007/s00268-008-9862-8
更新日期:2009-03-01 00:00:00
abstract::Congenital diaphragmatic hernia (CDH) is one of the most common causes of neonatal morbidity and mortality. The clinical spectrum of CDH ranges from minimally affected infants who do well with modern neonatal care to severely affected infants who die despite all interventions. Two decades of research have led to advan...
journal_title:World journal of surgery
pub_type: 杂志文章,评审
doi:10.1007/s00268-002-6739-0
更新日期:2003-01-01 00:00:00
abstract::Herniographic examinations were performed on more than 4,000 patients. The method was found to be of great value in patients with obscure symptoms in the groin or pelvis. Herniography shows not only hernial sacs but also the general condition and tissue strength of the inguinal part of the abdominal wall. The differen...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/BF01658871
更新日期:1989-09-01 00:00:00
abstract::Sixty-one patients who were diagnosed with mucosal gastric cancer have been successfully treated with two laparoscopic techniques at our institute from March 1992 to March 1997. One is laparoscopic wedge resection of the stomach using a lesion-lifting method for lesions of the anterior wall, the lesser curvature, and ...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/pl00013167
更新日期:1999-02-01 00:00:00
abstract::The most common causes of variceal bleeding are cirrhosis, schistosomiasis, and extrahepatic portal venous obstruction. The prognosis for an individual patient depends on the severity of the bleeding episode and the underlying liver function. Liver function is determined to a large extent by the underlying liver patho...
journal_title:World journal of surgery
pub_type: 杂志文章,评审
doi:10.1007/BF00294397
更新日期:1994-03-01 00:00:00
abstract:BACKGROUND:The 'gentle' handling of tissue (i.e., 'respect for tissue') is a fundamental aspect of surgical performance and learning. To date, there have been no methodological assessments that quantitatively measure 'gentleness.' Therefore, the aims of this study were (1) to propose a novel metric for gentle surgical ...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-019-05018-7
更新日期:2019-09-01 00:00:00
abstract::Percutaneous dilatational tracheostomy (PDT) is being increasingly used. Concerns have been raised as to its safety, especially when it is done at the bedside. A prospective evaluation was conducted of 100 consecutive, unselected critically ill patients with PDT. The mean intensive care unit (ICU) stay before PDT was ...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s002680010180
更新日期:2000-09-01 00:00:00
abstract::Surgical innovation relies on patient safety and quality of life, which require a drastic iatrogenic impact reduction. A parallel development toward less invasive approaches has occurred in the field of surgery, interventional radiology, and endoscopy. Minimally invasive techniques provide unquestionable benefits to p...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-014-2879-2
更新日期:2015-03-01 00:00:00