Demonstration of endogenous lectins in synovial tissue.

Abstract:

:We have recently shown that synoviocytes and extracellular matrices exhibit distinct patterns of carbohydrate expression. Their biological relevance is however not known. The purpose of the present study was to find out whether human synovial tissue would also show a specific receptor pattern for complex sugar molecules. Endogenous lectins were displayed by means of biotinylated neoglycoproteins and sulfated polysaccharides in paraffin-embedded material or cryosections. In addition to certain carbohydrate components that are known to be constituents of the carbohydrate part of cellular glycoconjugates, our panel included heparin and fucoidan, a sulfated fucose. Binding sites were shown using the avidin-peroxidase technique for light microscopy. The results were compared with immunohistochemical methods and enzyme histochemistry. Our study demonstrates that human synovial tissue contains a complex pattern of endogenous lectins depending on the different types of synovitis. The staining method we used in the investigation allows for precise localization of saccharide binding receptors and is therefore believed to be a reliable technique for further phenotypic characterization of synovial cells.

journal_name

Scand J Rheumatol

authors

Zschäbitz A,Gabius HJ,Stofft E,Reichel W

doi

10.3109/03009749109096796

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

242-51

issue

4

eissn

0300-9742

issn

1502-7732

journal_volume

20

pub_type

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