Overexpression of an auxilin-like gene (F9E10.5) can suppress Al uptake in roots of Arabidopsis.


:Plants resistant to aluminium (Al) stress were isolated from Arabidopsis thaliana enhancer-tagged mutant lines. Compared with the parental Col-7 control line, one of the resistant candidates, #355-2, showed a higher expression of the F9E10.5 gene (At1g75100) on chromosome 1, a lower Al content in whole roots, and a shorter root hair length (approximately 30%). Both Al influx and associated oxidative stress occurred in root hairs, as well as in root tips of Col-7; however, they were seen only in root tips of #355-2. Transgenic plants overexpressing the F9E10.5 gene showed a slightly higher Al resistance than their parental control line (Ler). The F9E10.5 gene encodes an auxilin-like protein related to the clathrin-uncoating process in endocytosis. Microscopic observation indicated that both Al ion influx and endocytosis activity were lower in root hair cells of the #355-2 line than in those of Col-7. These results suggested that overexpression of this auxilin-like protein inhibits endocytosis in root hair cells by a disturbance of the transport system as in animal cells shown previously. It was also suggested that a part of the Al influx occurred via endocytosis in root hair cells in Arabidopsis. The Al resistance in the #355-2 line may therefore be due to a lower Al uptake via endocytosis in the root hair region.


J Exp Bot


Ezaki B,Kiyohara H,Matsumoto H,Nakashima S




Has Abstract


2007-01-01 00:00:00














  • Proteomes of hard and soft near-isogenic wheat lines reveal that kernel hardness is related to the amplification of a stress response during endosperm development.

    abstract::Wheat kernel texture, a major trait determining the end-use quality of wheat flour, is mainly influenced by puroindolines. These small basic proteins display in vitro lipid binding and antimicrobial properties, but their cellular functions during grain development remain unknown. To gain an insight into their biologic...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Lesage VS,Merlino M,Chambon C,Bouchet B,Marion D,Branlard G

    更新日期:2012-01-01 00:00:00

  • Transcriptional and biochemical analyses of gibberellin expression and content in germinated barley grain.

    abstract::Mobilization of reserves in germinated cereal grains is critical for early seedling vigour, global crop productivity, and hence food security. Gibberellins (GAs) are central to this process. We have developed a spatio-temporal model that describes the multifaceted mechanisms of GA regulation in germinated barley grain...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Betts NS,Dockter C,Berkowitz O,Collins HM,Hooi M,Lu Q,Burton RA,Bulone V,Skadhauge B,Whelan J,Fincher GB

    更新日期:2020-03-25 00:00:00

  • Calculated activity of Mn2+ at the outer surface of the root cell plasma membrane governs Mn nutrition of cowpea seedlings.

    abstract::Manganese (Mn) is an essential micronutrient for plant growth but is often toxic in acid or waterlogged soils. Using cowpea (Vigna unguiculata L. Walp.) grown with 0.05-1500 μM Mn in solution, two short-term (48 h) solution culture experiments examined if the effects of cations (Ca, Mg, Na, Al, or H) on Mn nutrition a...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Kopittke PM,Blamey FP,Wang P,Menzies NW

    更新日期:2011-07-01 00:00:00

  • Signalling of Arabidopsis thaliana response to Pieris brassicae eggs shares similarities with PAMP-triggered immunity.

    abstract::Insect egg deposition activates plant defence, but very little is known about signalling events that control this response. In Arabidopsis thaliana, oviposition by Pieris brassicae triggers salicylic acid (SA) accumulation and induces the expression of defence genes. This is similar to the recognition of pathogen-asso...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Gouhier-Darimont C,Schmiesing A,Bonnet C,Lassueur S,Reymond P

    更新日期:2013-01-01 00:00:00

  • Xylem tension affects growth-induced water potential and daily elongation of maize leaves.

    abstract::Diurnal rates of leaf elongation vary in maize (Zea mays L.) and are characterized by a decline each afternoon. The cause of the afternoon decline was investigated. When the atmospheric environment was held constant in a controlled environment, and water and nutrients were adequately supplied to the soil or the roots ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Tang AC,Boyer JS

    更新日期:2008-01-01 00:00:00

  • Isolation of Arabidopsis ahg11, a weak ABA hypersensitive mutant defective in nad4 RNA editing.

    abstract::The phytohormone abscisic acid (ABA) plays pivotal roles in the regulation of developmental and environmental responses in plants. Identification of cytoplasmic ABA receptors enabled the elucidation of the main ABA signalling pathway, connecting ABA perception to either nuclear events or the action of several transpor...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Murayama M,Hayashi S,Nishimura N,Ishide M,Kobayashi K,Yagi Y,Asami T,Nakamura T,Shinozaki K,Hirayama T

    更新日期:2012-09-01 00:00:00

  • Hydraulic conductance as well as nitrogen accumulation plays a role in the higher rate of leaf photosynthesis of the most productive variety of rice in Japan.

    abstract::An indica variety Takanari is known as one of the most productive rice varieties in Japan and consistently produces 20-30% heavier dry matter during ripening than Japanese commercial varieties in the field. The higher rate of photosynthesis of individual leaves during ripening has been recognized in Takanari. By using...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Taylaran RD,Adachi S,Ookawa T,Usuda H,Hirasawa T

    更新日期:2011-07-01 00:00:00

  • VpRFP1, a novel C4C4-type RING finger protein gene from Chinese wild Vitis pseudoreticulata, functions as a transcriptional activator in defence response of grapevine.

    abstract::RING finger proteins comprise a large family and play important roles in regulation of growth and development, hormone signalling, and responses to biotic and abiotic stresses in plants. In this study, the identification and functional characterization of a C4C4-type RING finger protein gene from the Chinese wild grap...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Yu Y,Xu W,Wang S,Xu Y,Li H,Wang Y,Li S

    更新日期:2011-11-01 00:00:00

  • Hydraulic properties of individual xylem vessels of Fraxinus americana.

    abstract::Studies of the hydraulic properties of xylem vessels have been limited to measurements of whole plant or whole stem segments. This approach allows the longitudinal transport properties of the ensemble of vessels within a stem to be determined, but provides little information on radial transport. Here the xylem of Frax...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Zwieniecki MA,Melcher PJ,Holbrook NM

    更新日期:2001-02-01 00:00:00

  • miR156-targeted SPL10 controls Arabidopsis root meristem activity and root-derived de novo shoot regeneration via cytokinin responses.

    abstract::Root growth is modulated by different factors, including phytohormones, transcription factors, and microRNAs (miRNAs). MicroRNA156 and its targets, the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) genes, define an age-dependent pathway that controls several developmental processes, including lateral root emergence. Ho...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Barrera-Rojas CH,Rocha GHB,Polverari L,Pinheiro Brito DA,Batista DS,Notini MM,da Cruz ACF,Morea EGO,Sabatini S,Otoni WC,Nogueira FTS

    更新日期:2020-01-23 00:00:00

  • Identification and characterization of Arabidopsis AtNUDX9 as a GDP-d-mannose pyrophosphohydrolase: its involvement in root growth inhibition in response to ammonium.

    abstract::GDP-d-mannose (GDP-d-Man) is an important intermediate in ascorbic acid (AsA) synthesis, cell wall synthesis, protein N-glycosylation, and glycosylphosphatidylinositol-anchoring in plants. Thus, the modulation of intracellular levels of GDP-d-Man could be important for maintaining various cellular processes. Here an A...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Tanaka H,Maruta T,Ogawa T,Tanabe N,Tamoi M,Yoshimura K,Shigeoka S

    更新日期:2015-09-01 00:00:00

  • Effect of breeding on nitrogen use efficiency-associated traits in oilseed rape.

    abstract::Oilseed rape is one of the most important dicotyledonous field crops in the world, where it plays a key role in productive cereal crop rotations. However, its production requires high nitrogen fertilization and its nitrogen footprint exceeds that of most other globally important crops. Hence, increased nitrogen use ef...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Stahl A,Vollrath P,Samans B,Frisch M,Wittkop B,Snowdon RJ

    更新日期:2019-03-27 00:00:00

  • Osmotic stress-induced polyamine oxidation mediates defence responses and reduces stress-enhanced grapevine susceptibility to Botrytis cinerea.

    abstract::Abiotic factors inducing osmotic stress can influence the plant immune response and resistance to pathogen infections. In this study, the effect of polyethylene glycol (PEG)- and sucrose-induced osmotic stress on polyamine (PA) homeostasis and the basal immune response in grapevine plantlets before and after Botrytis ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Hatmi S,Trotel-Aziz P,Villaume S,Couderchet M,Clément C,Aziz A

    更新日期:2014-01-01 00:00:00

  • A link between magnesium-chelatase H subunit and sucrose nonfermenting 1 (SNF1)-related protein kinase SnRK2.6/OST1 in Arabidopsis guard cell signalling in response to abscisic acid.

    abstract::Magnesium-chelatase H subunit [CHLH/putative abscisic acid (ABA) receptor ABAR] positively regulates guard cell signalling in response to ABA, but the molecular mechanism remains largely unknown. A member of the sucrose nonfermenting 1 (SNF1)-related protein kinase 2 family, SnRK2.6/open stomata 1 (OST1)/SRK2E, which ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Liang S,Lu K,Wu Z,Jiang SC,Yu YT,Bi C,Xin Q,Wang XF,Zhang DP

    更新日期:2015-10-01 00:00:00

  • Approaches to improve soil fertility in sub-Saharan Africa.

    abstract::Soil fertility provides the foundation for nutritious food production and resilient and sustainable livelihoods. A comprehensive survey and summit meeting were conducted with the aims of understanding barriers to enhancing soil fertility in sub-Saharan Africa and providing evidence-based recommendations. The focus reg...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Stewart ZP,Pierzynski GM,Middendorf BJ,Prasad PVV

    更新日期:2020-01-07 00:00:00

  • Pressure gradients along whole culms and leaf sheaths, and other aspects of humidity-induced gas transport in Phragmites australis.

    abstract::Emergent aquatic macrophytes growing in waterlogged anaerobic sediments overlain by deep water require particularly efficient ventilating systems. In Phragmites australis (Cav.) Trin. ex Steud, pressurized gas flows, generated by humidity-induced diffusion of air into leaf sheaths, enhance oxygen transport to below-gr...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Afreen F,Zobayed SM,Armstrong J,Armstrong W

    更新日期:2007-01-01 00:00:00

  • The rheology of a growing leaf: stress-induced changes in the mechanical properties of leaves.

    abstract::We study in situ the mechanics and growth of a leaf. Young Nicotiana tabacum leaves respond to applied mechanical stress by altering both their mechanical properties and the characteristics of their growth. We observed two opposite behaviours, each with its own typical magnitude and timescale. On timescales of the ord...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Sahaf M,Sharon E

    更新日期:2016-10-01 00:00:00

  • Diel patterns of leaf and root growth: endogenous rhythmicity or environmental response?

    abstract::Plants are sessile organisms forced to adjust to their surrounding environment. In a single plant the photoautotrophic shoot is exposed to pronounced environmental variations recurring in a day-night 24 h (diel) cycle, whereas the heterotrophic root grows in a temporally less fluctuating environment. The contrasting h...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Ruts T,Matsubara S,Wiese-Klinkenberg A,Walter A

    更新日期:2012-05-01 00:00:00

  • A comparative study of ripening among berries of the grape cluster reveals an altered transcriptional programme and enhanced ripening rate in delayed berries.

    abstract::Transcriptional studies in relation to fruit ripening generally aim to identify the transcriptional states associated with physiological ripening stages and the transcriptional changes between stages within the ripening programme. In non-climacteric fruits such as grape, all ripening-related genes involved in this pro...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Gouthu S,O'Neil ST,Di Y,Ansarolia M,Megraw M,Deluc LG

    更新日期:2014-11-01 00:00:00

  • Expression of Arabidopsis class 1 phytoglobin (AtPgb1) delays death and degradation of the root apical meristem during severe PEG-induced water deficit.

    abstract::Maintenance of a functional root is fundamental to plant survival in response to some abiotic stresses, such as water deficit. In this study, we found that overexpression of Arabidopsis class 1 phytoglobin (AtPgb1) alleviated the growth retardation of polyethylene glycol (PEG)-induced water stress by reducing programm...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Mira MM,Huang S,Kapoor K,Hammond C,Hill RD,Stasolla C

    更新日期:2017-11-28 00:00:00

  • Iron uptake system mediates nitrate-facilitated cadmium accumulation in tomato (Solanum lycopersicum) plants.

    abstract::Nitrogen (N) management is a promising agronomic strategy to minimize cadmium (Cd) contamination in crops. However, it is unclear how N affects Cd uptake by plants. Wild-type and iron uptake-inefficient tomato (Solanum lycopersicum) mutant (T3238fer) plants were grown in pH-buffered hydroponic culture to investigate t...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Luo BF,Du ST,Lu KX,Liu WJ,Lin XY,Jin CW

    更新日期:2012-05-01 00:00:00

  • The effect of sucrose and abscisic acid interaction on sucrose synthase and its relationship to grain filling of rice (Oryza sativa L.).

    abstract::Rice grain filling is a process of conversion of sucrose into starch catalysed by a series of enzymes. Sucrose synthase (SUS) is considered as a key enzyme regulating this process. This study investigated the possible roles of sucrose and abscisic acid (ABA) in mediating the activity and expression of SUS protein of g...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Tang T,Xie H,Wang Y,Lü B,Liang J

    更新日期:2009-01-01 00:00:00

  • Multispectral fluorescence and reflectance imaging at the leaf level and its possible applications.

    abstract::Images taken at different spectral bands are increasingly used for characterizing plants and their health status. In contrast to conventional point measurements, imaging detects the distribution and quantity of signals and thus improves the interpretation of fluorescence and reflectance signatures. In multispectral fl...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Lenk S,Chaerle L,Pfündel EE,Langsdorf G,Hagenbeek D,Lichtenthaler HK,Van Der Straeten D,Buschmann C

    更新日期:2007-01-01 00:00:00

  • Long-distance signalling from roots to shoots assessed: the flooding story.

    abstract::Several kinds of signal may be generated when roots are exposed to an environmental stress. Some, but not all, are conveyed to shoots in the transpiration stream. Principles are summarized that may help establish experimentally the presence or intensity of root signals transported by transpiration. In many dryland spe...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Jackson MB

    更新日期:2002-02-01 00:00:00

  • Low temperature maximizes growth of Crocus vernus (L.) Hill via changes in carbon partitioning and corm development.

    abstract::In Crocus vernus, a spring bulbous species, prolonged growth at low temperatures results in the development of larger perennial organs and delayed foliar senescence. Because corm growth is known to stop before the first visual sign of leaf senescence, it is clear that factors other than leaf duration alone determine f...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Lundmark M,Hurry V,Lapointe L

    更新日期:2009-01-01 00:00:00

  • Rutin in buckwheat herbs grown at different UV-B radiation levels: comparison of two UV spectrophotometric and an HPLC method.

    abstract::Rutin is an antioxidant with many interesting pharmacological effects. It can also be found in buckwheat (Fagopyrum esculentum Moench). UV radiation stimulates the activity of enzymes of the phenylpropanoid pathway and there is some evidence that it influences the rutin content in plants. The aim of the present resear...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Kreft S,Strukelj B,Gaberscik A,Kreft I

    更新日期:2002-08-01 00:00:00

  • The AMI1 gene family: indole-3-acetamide hydrolase functions in auxin biosynthesis in plants.

    abstract::Novel genes that function in the conversion of indole-3-acetamide (IAM) into indole-3-acetic acid (IAA), which were previously thought to exist only in the bacterial genome, have been isolated from plants. The finding of the AtAMI1 gene in Arabidopsis thaliana and the NtAMI1 gene in Nicotiana tabacum, which encode ind...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Mano Y,Nemoto K,Suzuki M,Seki H,Fujii I,Muranaka T

    更新日期:2010-01-01 00:00:00

  • Small RNA activity and function in angiosperm gametophytes.

    abstract::Small non-coding RNAs are key post-transcriptional and transcriptional regulators of plant gene expression in angiosperm sporophytes. In recent years, gametophytic small RNAs have also been investigated, predominantly in Arabidopsis male gametophytes, revealing features in common with the sporophyte as well as some su...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Le Trionnaire G,Grant-Downton RT,Kourmpetli S,Dickinson HG,Twell D

    更新日期:2011-03-01 00:00:00

  • Distinct metabolic pathways drive monoterpenoid biosynthesis in a natural population of Pelargonium graveolens.

    abstract::Pelargonium graveolens is a wild predecessor to rose-scented geranium hybrids prized for their essential oils used as fragrances and flavorings. However, little is known about their biosynthesis. Here we present metabolic evidence that at least two distinct monoterpene biosynthetic pathways contribute to their volatil...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Bergman ME,Chávez Á,Ferrer A,Phillips MA

    更新日期:2020-01-01 00:00:00

  • Resistances along the CO2 diffusion pathway inside leaves.

    abstract::CO(2) faces a series of resistances while diffusing between the substomatal cavities and the sites of carboxylation within chloroplasts. The absence of techniques to measure the resistance of individual steps makes it difficult to define their relative importance. Resistance to diffusion through intercellular airspace...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Evans JR,Kaldenhoff R,Genty B,Terashima I

    更新日期:2009-01-01 00:00:00