Abstract:
BACKGROUND & AIMS:The Raf kinase inhibitor protein (RKIP) has been identified as a suppressor of the mitogen-activated protein kinase (MAPK) pathway. Loss of RKIP function promotes tumor metastasis in prostate cancer and melanoma. The insulin-like growth factor I (IGF-I)-mediated MAPK cascade is often activated in hepatocellular carcinoma (HCC), but the role of RKIP in the molecular pathogenesis of these tumors is unknown. This study was performed to evaluate the role of RKIP in the development of HCC. METHODS:The levels of RKIP expression in HCC tumor and corresponding peritumoral tissues were determined by immunohistochemistry and Western blot analysis. The underlying mechanisms of RKIP were assessed with immunoblot analysis, Raf kinase activity assay, cell proliferation, and migration assays after either overexpression or knockdown of RKIP expression in HCC cell lines. RESULTS:RKIP expression is down-regulated in human HCC compared with adjacent peritumoral tissues. Low RKIP levels were correlated with enhanced extracellular signal-regulated-kinase (ERK)/MAPK pathway activation. Reconstitution experiments antagonized IGF-I-mediated MAPK pathway activation, resulting in reduced nuclear accumulation of phospho-ERK. In contrast, knockdown of RKIP expression using small interfering RNA induced activation of the ERK/MAPK pathway. Ectopic expression of RKIP altered HCC cell proliferation and migration. CONCLUSIONS:Our findings indicate that down-regulation of RKIP expression is a major factor in activation of the IGF-I/ERK/MAPK pathway during human hepatocarcinogenesis.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Lee HC,Tian B,Sedivy JM,Wands JR,Kim Mdoi
10.1053/j.gastro.2006.07.012subject
Has Abstractpub_date
2006-10-01 00:00:00pages
1208-17issue
4eissn
0016-5085issn
1528-0012pii
S0016-5085(06)01537-Xjournal_volume
131pub_type
杂志文章相关文献
GASTROENTEROLOGY文献大全abstract::Liver disease with inclusions of copper was recognized among 30 rural workers with "vineyard sprayer's lung." The pathological findings were varied: focal or diffuse swelling and proliferation of Kupffer cells; histiocytic and sarcoid-like granulomata; fibrosis of variable degree in the perisinusoidal, portal, and sub...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1977-02-01 00:00:00
abstract::Decreases in arterial pH markedly increase sodium, chloride, and water absorption in the normal ileum and can reverse ongoing cholera toxin-induced secretion. In the current study we examined whether these effects of pH are evident in other models of ileal secretion, and in a model of increased absorption. Rats were a...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(89)91556-4
更新日期:1989-02-01 00:00:00
abstract:BACKGROUND & AIMS:The effects of aging on pancreatic acinar cell proliferation have not been clearly defined. Phosphatidylinositol 3-kinase (PI3K)-mediated phosphorylation of Akt is a critical step for proliferation of various cell types and insulin secretion from pancreatic endocrine cells; however, its role in acinar...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2005.03.016
更新日期:2005-05-01 00:00:00
abstract::Adverse effects associated with nonsteroidal antiinflammatory drug treatment are reported more commonly to regulatory authorities than the adverse effects of any other form of treatment. Epidemiologic evidence in general suggests a doubling or quadrupling of the risk of ulcer complications or death in recipients of su...
journal_title:Gastroenterology
pub_type: 杂志文章,评审
doi:10.1016/s0016-5085(89)80060-5
更新日期:1989-02-01 00:00:00
abstract::Hepatitis C virus (HCV) is the agent responsible for posttransfusion hepatitis. The incidence, timing, and clinical course of HCV positive hepatitis in liver transplant recipients are unknown. Three hundred and seventeen donor-recipient liver transplant pairs were grouped on the basis of their pretransplant HCV antibo...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(92)91130-v
更新日期:1992-07-01 00:00:00
abstract:BACKGROUND & AIMS:Cell stress signaling pathways result in phosphorylation of the eukaryotic translation initiation factor 2 subunit alpha (EIF2S1 or EIF2A), which affects regulation of protein translation. Translation reprogramming mitigates stress by activating pathways that result in autophagy and cell death, to eli...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2017.12.016
更新日期:2018-04-01 00:00:00
abstract::Based on in vitro experiments measuring daily secretion rates in the culture media of rat hepatocytes and in vivo experiments using pulse labeling of intracellular precursors, the present study examines the ontogenic expression of the polymeric immunoglobulin receptor and secretory component by hepatocytes during grow...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(92)90181-w
更新日期:1992-03-01 00:00:00
abstract::A patient is reported with eosinophilic enteritis of the jejunum with fatal evolution. Surgical resection was considered impossible and medical treatment with corticosteroids, elemental diet, parenteral nutrition, and antimicrobials to combat the intestinal bacterial overgrowth failed to control the disease. The destr...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1976-09-01 00:00:00
abstract::Disappearance of symptoms, resolution of most biochemical abnormalities, and histologic improvement to mild chronic inflammation were accomplished in 69 of 123 patients (56%) with severe chronic active liver disease treated with corticosteroids for up to 6.5 yr. Remission of at least 6 mo duration was possible in 35 o...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1980-03-01 00:00:00
abstract::To test whether bile acids interact with mast cells, dilute, aqueous solutions of five pure unconjugated natural bile acids and their corresponding glycine or taurine conjugates were incubated with murine PT-18 cells (a mast cell line functionally and cytochemically similar to mucosal mast cells) or with freshly isola...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(91)90024-f
更新日期:1991-08-01 00:00:00
abstract:BACKGROUND/AIMS:Butyrate and factor XIII may improve ulcerative colitis; they also affect tissue and serum transglutaminase levels. We investigated the therapeutic potential of sodium butyrate and factor XIII and the role of transglutaminase during mucosal repair in experimental colitis. METHODS:Rats with induced coli...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(94)90598-3
更新日期:1994-02-01 00:00:00
abstract::Interleukin 1 (IL-1) has been shown to reduce the severity of experimental gastroduodenal ulceration, but the mechanism of action is unclear. The present study examined the possibility that the mechanism underlying the protective effects of IL-1 in experimental nonsteroidal anti-inflammatory drug (NSAID)-induced gastr...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract:BACKGROUND & AIMS:Hereditary diffuse gastric cancer (HDGC) families carry CDH1 heterozygous germline mutations; their tumors acquire complete CDH1 inactivation through "2nd-hit" mechanisms. Most frequently, this occurs via promoter hypermethylation (epigenetic modification), and less frequently via CDH1 mutations and l...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2009.02.065
更新日期:2009-06-01 00:00:00
abstract:BACKGROUND & AIMS:Corticotropin-releasing factor (CRF) signaling pathways play a key role in the stress response through the activation of CRF(1) and CRF(2) receptors. We investigated the CRF receptor subtypes involved in gastric postoperative ileus. METHODS:Adult male mice (C57BL/6, CRF(1)-deficient, and wild-type), ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(03)01069-2
更新日期:2003-09-01 00:00:00
abstract:BACKGROUND & AIMS:Simvastatin improves liver generation of nitric oxide and hepatic endothelial dysfunction in patients with cirrhosis, so it could be an effective therapy for portal hypertension. This randomized controlled trial evaluated the effects of continuous simvastatin administration on the hepatic venous press...
journal_title:Gastroenterology
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1053/j.gastro.2009.01.043
更新日期:2009-05-01 00:00:00
abstract::These studies were designed to better understand the effects of luminal nutrition on intestinal mass and function. Parenterally nourished rats received a midjejunal infusion of either 0.9% saline, 10% glucose, 10% 3-O-methyl glucose, or 30% glucose. A fifth group underwent sham operation. After 7 days, intestinal mass...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00
abstract::We investigated a hormonal mechanism in a trypsin inhibitor-induced pancreatic hypersecretion in rats. Intraduodenal administration of a synthetic trypsin inhibitor, camostat, resulted in significant increases in plasma concentration of both secretin and cholecystokinin in a dose-related manner that paralleled a signi...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(92)90111-b
更新日期:1992-02-01 00:00:00
abstract::We have conducted a double-blind controlled trial of colchicine in patients with primary biliary cirrhosis. Fifty-seven patients with biopsy-proven primary biliary cirrhosis were randomized to receive either 0.6 mg of colchicine twice daily or an identically appearing placebo. Patients underwent clinical and laborator...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0016-5085(88)90300-9
更新日期:1988-07-01 00:00:00
abstract::The aim of this study was to test in vivo (a) whether the sphincter of Oddi acts as a resistor or also as a pump, (b) the effect of an IV infusion of cholecystokinin (CCK) on choledochoduodenal flow, and (c) the ability of the choledochoduodenal junction to prevent duodenobiliary reflux in two animal species, opossums...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(90)90469-h
更新日期:1990-12-01 00:00:00
abstract:BACKGROUND & AIMS:Guidelines for the management of venous thromboembolism (VTE) from the American College of Chest Physicians do not address patients with inflammatory bowel disease (IBD), a group with a high risk of both VTE and gastrointestinal bleeding. We present recommendations for the prevention and treatment of ...
journal_title:Gastroenterology
pub_type: 共识发展会议,杂志文章,评审
doi:10.1053/j.gastro.2014.01.042
更新日期:2014-03-01 00:00:00
abstract:BACKGROUND & AIMS:There are intra- and interobserver variations in endoscopic assessment of ulcerative colitis (UC) and biopsies are often collected for histologic evaluation. We sought to develop a deep neural network system for consistent, objective, and real-time analysis of endoscopic images from patients with UC. ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2020.02.012
更新日期:2020-06-01 00:00:00
abstract::Using flowing isotonic NaCl or flowing molar KCl electrodes, the magnitude of liquid junction potentials between the electrode tip and gastric juice was measured directly, and calculated from electrolyte concentrations in gastric juice according to standard formulas. Gastric transepithelial potential difference was ob...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1979-05-01 00:00:00
abstract::Weight gain and obesity are major risk factors for conditions and diseases ranging from insulin resistance and type 2 diabetes mellitus to atherosclerosis and the sequelae of nonalcoholic fatty liver disease. A chronic, subacute state of inflammation often accompanies the accumulation of excess lipid in adipose tissue...
journal_title:Gastroenterology
pub_type: 杂志文章,评审
doi:10.1053/j.gastro.2007.03.059
更新日期:2007-05-01 00:00:00
abstract::The range of activity and the location of lipase and pepsin were determined in the stomach and duodenum of infants, children, and adults. The range of lipase activity in biopsy specimens from the gastric body, in 29 subjects aged from 3 months to 26 years, was 1.8-5.3 U/mg protein (1 U is 1 mumol [3H]oleic acid releas...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(91)90467-y
更新日期:1991-07-01 00:00:00
abstract:BACKGROUND & AIMS:To date, computed tomographic (CT) colonography has been compared with an imperfect test, colonoscopy, and has been mainly assessed in patients with positive screening test results or symptoms. Therefore, the available data may not apply to screening of patients with a personal or family history of co...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2004.03.055
更新日期:2004-07-01 00:00:00
abstract::The results of a clinical trial comparing slow-release 5-aminosalicylic acid tablets (Pentasa) and enteric-coated sulfasalazine tablets (Salazopyrin) with regard to the efficacy of maintaining ulcerative colitis patients in remission for 12 mo and with regard to safety of treatment are reported. Seventy-five patients ...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1016/s0016-5085(88)80061-1
更新日期:1988-12-01 00:00:00
abstract::To study the peripheral effect of somatostatin in dogs a tiny Silastic catheter was implanted in an almost terminal branch of the mesenteric artery, providing blood supply to a jejunal segment of 5-10 cm. In 4 dogs one catheter was implanted, perfusing a small bowel segment about 60 cm below the angle of Treitz; in 3 ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1984-11-01 00:00:00
abstract::Identification of physiologic factors that predict response to fecal incontinence therapy would be helpful in choosing the optimal treatment and advising patients on the likelihood of a successful outcome. However, few physiologic parameters have been consistently identified as important in predicting response to eith...
journal_title:Gastroenterology
pub_type: 杂志文章,评审
doi:10.1053/j.gastro.2003.10.008
更新日期:2004-01-01 00:00:00
abstract::A rapid, sensitive, precise radioimmunoassay for conjugates of chenodeoxycholic (chenic) acid has been developed and validated. Immunogen was prepared with chenylglycine couples to bovine serum albumin, emulsified in Freund's complete adjuvant, and injected intracutaneously in rabbits. Antibodies of moderate titer (us...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1977-08-01 00:00:00
abstract:BACKGROUND & AIMS:Galectins are beta-galactoside-binding proteins possibly involved in tumor progression. The aim of this study was to determine the pattern of galectin 3 and galectin 1 expression and involvement in colorectal cancer progression. METHODS:Galectin 3 expression was examined immunohistochemically in 39 s...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(97)70010-6
更新日期:1997-12-01 00:00:00