Abstract:
BACKGROUND:The objective of this study was to determine whether rat insulin promoter (RIP) could, in a mouse model, direct expression of an adenovirus-mediated reporter gene specifically into pancreatic islets via systemic delivery. METHODS:Five hundred and eight base pairs of the RIP DNA sequence were constructed into an adenoviral vector containing a lacZ reporter gene (Adeno-RIP-lacZ). The cytomegalovirus (CMV) promoter was constructed to drive lacZ reporter-gene expression (Adeno-CMV-lacZ) and used as controls. In vitro transient transfection assays were performed to determine levels of reporter-gene expression and compared with that of liposome-mediated plasmid transfection. SCID mice were bred and housed in the barrier BL-4 animal facility. At 2 months of age, the human pancreatic cancer cell PANC-1 was intraperitoneally injected into male mice. Two months after the tumor cell inoculation, mice were injected with 10(7) adenoviral particles via tail veins. After gene delivery, mice were sacrificed at different time points to determine transgene expression levels. Complete necropsies were performed. Morphological alterations were determined using hematoxylin and eosin (H&E) staining, and distribution of the reporter lacZ gene was determined by immunohistochemistry analyses. RESULTS:Adenoviral-driven reporter-gene expression resulted in more than 5 times higher transgene expression compared with conventional plasmid transfections. In Adeno-RIP-lacZ-injected mice, lacZ expression was specifically detected in pancreatic islets. By contrast, in Adeno-CMV-lacZ-injected mice, lacZ gene expression was observed in multiple organs and tissues. Mononuclear cell infiltration and liver cell inflammation were found in Adeno-CMV-lacZ-treated mice. Similar phenomena were observed in islet cells of Adeno-RIP-lacZ-treated mice. A significantly higher level of reporter-gene expression was also found at the edge of in-vivo-inoculated human pancreatic tumors. CONCLUSION:These results demonstrate that RIP-directed reporter-gene expression was found specifically in mouse pancreatic islets and implanted human pancreatic cancer cells. These data thus demonstrate that the combination of an adenoviral vector and a tissue-specific promoter could lead to an enhanced and more specific transgene expression in vivo.
journal_name
World J Surgjournal_title
World journal of surgeryauthors
Wang X,Olmsted-Davis E,Davis A,Liu S,Li Z,Yang J,Brunicardi FCdoi
10.1007/s00268-005-0688-3subject
Has Abstractpub_date
2006-08-01 00:00:00pages
1543-52issue
8eissn
0364-2313issn
1432-2323journal_volume
30pub_type
杂志文章abstract:BACKGROUND:Gastrointestinal (GI) tract perforation during endoscopy is a rare but severe complication. The aim of this study was to determine predictors of morbidity and mortality after iatrogenic endoscopic perforation. MATERIALS AND METHODS:All cases with iatrogenic endoscopic perforation receiving surgery at a tert...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-017-3986-7
更新日期:2017-08-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:We determined the rates of initial lymph node metastasis following curative resection of mass-forming type-intrahepatic cholangiocarcinoma (ICC) in patients with and without hepatitis virus infection. METHODS:We enrolled 87 patients between January 2000 and December 2013 with ICC without preo...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-016-3843-0
更新日期:2017-04-01 00:00:00
abstract::It is a fundamental clinical observation that wounds do not heal in tissue that does not bleed, and they almost always heal in tissue that bleeds extensively. Continuous supply of oxygen to the tissue through microcirculation is vital for the healing process and for resistance to infection. Evaluation of tissue perfus...
journal_title:World journal of surgery
pub_type: 杂志文章,评审
doi:10.1007/s00268-003-7398-5
更新日期:2004-03-01 00:00:00
abstract:BACKGROUND:The Mangled Extremity Severity Score (MESS) was constructed as an objective quantification criterion for limb trauma. A MESS of or greater than 7 was proposed as a cut-off point for primary limb amputation. Opinions concerning the predictive value of the MESS vary broadly in the literature. The aim of this s...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-019-05263-w
更新日期:2020-03-01 00:00:00
abstract:BACKGROUND:Intestinal epithelial cell apoptosis has been reported in sepsis as a mechanism of organ failure. The aim of this study was to clarify the role of apoptosis-regulating proteins (bcl-2, bax, cytochrome-c, and caspase-8) in septic rats by studying their expression in gastric and intestinal epithelial cells. M...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-005-0742-1
更新日期:2007-04-01 00:00:00
abstract:BACKGROUND:Seroma formation is a frequent postoperative complication following open ventral hernia repair (OVHR), especially in cases requiring wide subcutaneous dissection (WSD). The aim of this study was to evaluate the effectiveness of a new low-thrombin fibrin sealant for seroma prevention. METHODS:A total of 60 c...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-014-2691-z
更新日期:2014-11-01 00:00:00
abstract::This retrospective study reports our experience managing 78 patients with adenocarcinoma of the esophagus and gastroesophageal junction operated between January 1982 and December 1996. Altogether 18 patients presented with stage I and II disease, and 60 patients had stage III and IV disease at presentation; 56 patient...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/pl00013179
更新日期:1999-03-01 00:00:00
abstract:IMPORTANCE:In resource-limited settings, identification of successful and sustainable task-shifting interventions is important for improving care. OBJECTIVE:To determine whether the training of lay people to take vital signs as trauma clerks is an effective and sustainable method to increase availability of vital sign...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-017-4121-5
更新日期:2017-12-01 00:00:00
abstract:BACKGROUND:The objective of the present study was to identify logistic regression models with better survival prediction than the Trauma and Injury Severity Score (TRISS) method in assessing blunt trauma (BT) victims in Japan and Thailand. An additional aim was to demonstrate the feasibility of probability of survival ...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-012-1498-z
更新日期:2012-04-01 00:00:00
abstract:BACKGROUND:Hepatic trisectionectomy is a challenge for surgeons. We describe a technique by combination of the Glisson pedicle method and anterior approach to perform hepatic trisectionectomy. METHODS:In this technique, the Glisson pedicles are isolated above the hilar plate without hilar dissection. After dividing th...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-014-2579-y
更新日期:2014-09-01 00:00:00
abstract:BACKGROUND:Ghrelin is a brain-gut peptide with GH-releasing and appetite-inducing properties. Because ghrelin is secreted mainly by the stomach, fasting levels fall after distal gastrectomy. The vagal nerve is responsible for periprandial changes. The presents study investigated the impact of preserving the celiac bran...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-013-2078-6
更新日期:2013-09-01 00:00:00
abstract:BACKGROUND:The albumin-bilirubin (ALBI) score was initially developed for assessing liver dysfunction severity and was suggested to have prognostic value in patients with hepatocellular carcinoma. We aimed to evaluate the prognostic impact of ALBI grade in patients with advanced gastric cancer (GC) after radical gastre...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-017-4234-x
更新日期:2018-03-01 00:00:00
abstract::Met protein is a tyrosine kinase receptor for hepatocyte growth factor (HGF). c-Met has morphogenic, mitogenic, and motogenic properties and is overexpressed in many solid tumors. We studied c-met mRNA and protein expression in papillary thyroid carcinomas and nonneoplastic thyroid tissue. The c-met mRNA was detected ...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s002680020167
更新日期:2001-05-01 00:00:00
abstract:BACKGROUND:Patients with end-stage renal disease (ESRD) and secondary hyperparathyroidism (SHPT) are at high risk of mortality. Whether an increased risk of death persists after a parathyroidectomy (PTX) is not clearly established. SUBJECTS AND METHODS:The survival of 40 patients with ESRD and SHPT who underwent PTX w...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-006-0693-1
更新日期:2007-05-01 00:00:00
abstract::Necrotizing lesions of the soft tissues are grave entities not infrequently seen in daily surgical practice. They may occur with epidemic proportions after natural disasters, representing a serious challenge to the surgeon since they are characteristically associated with high mortality rates unless an early diagnosis...
journal_title:World journal of surgery
pub_type: 杂志文章,评审
doi:10.1007/BF01659058
更新日期:1991-03-01 00:00:00
abstract:BACKGROUND:The Laboratory Risk Indicator for Necrotizing Fasciitis (LRINEC) scoring system was developed to aid the diagnosis of necrotizing fasciitis and guide management [1]. AIM:To validate the LRINEC score and identify clinical predictors to develop a refined diagnostic scoring tool for the diagnosis of necrotizin...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-019-05061-4
更新日期:2019-10-01 00:00:00
abstract::There are now excellent data that patients with stage III colon cancer or stage II and III rectal cancer live longer if they receive, respectively, systemic or regional and systemic therapy after surgery. In addition, disease-free survival, particularly freedom from the symptoms of regional recurrence, in the high ris...
journal_title:World journal of surgery
pub_type: 杂志文章,评审
doi:10.1007/BF00308633
更新日期:1995-03-01 00:00:00
abstract:BACKGROUND:We assessed whether the simultaneous sequential strategy could (1) achieve additional sinus restoration for those patients who were not in sinus rhythm while coming off bypass after modified left maze procedure and (2) attain the same long-term success rates as the bi-atrial maze procedure in patients with p...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-006-0382-0
更新日期:2006-10-01 00:00:00
abstract:BACKGROUND:Although ultrasonographic (US) evaluation is currently recommended for the workup of all palpable thyroid nodules, little guidance exists regarding the manner in which US data are to be used. Herein, we examine the available evidence and recommend how US can be used most effectively to predict malignancy. We...
journal_title:World journal of surgery
pub_type: 杂志文章,评审
doi:10.1007/s00268-008-9494-z
更新日期:2008-07-01 00:00:00
abstract:OBJECTIVES:The purpose of this study was to evaluate the feedback of participants upon laparoscopic liver surgery (LLS) course on Thiel-embalmed human bodies. METHODS:From 2010 to 2017, ten LLS masterclasses have been organized by the Department of Hepatobiliary Surgery at Ghent University Hospital. A 23-question anon...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-019-05103-x
更新日期:2019-11-01 00:00:00
abstract::To evaluate quality of life and functional results following surgery for gastric cancer we studied 104 patients with no evidence of disease at a minimum of 12 months postoperatively. Patients were treated with total gastrectomy and jejunal pouch reconstruction according to Hunt-Lawrence-Rodino (n = 59) or simple esoph...
journal_title:World journal of surgery
pub_type: 临床试验,杂志文章
doi:10.1007/BF00294722
更新日期:1995-07-01 00:00:00
abstract::The combination of conventional traumatic injuries and radiation exposure has synergistic consequences, the full extent of which may take days to weeks to become apparent. Our understanding of such is derived from a variety of laboratory and clinical scenarios involving both therapeutic and accidental exposures. When ...
journal_title:World journal of surgery
pub_type: 杂志文章,评审
doi:10.1007/BF02066992
更新日期:1992-09-01 00:00:00
abstract:BACKGROUND:Inguinal hernia is thought to be common in rural Ghana, though no recent data exist on hernia prevalence in the country. This information is needed to guide policy and increase access to safe hernia repair in Ghana and other low-resource settings. METHODS:Adult men randomly selected from the Barekese sub-di...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-015-3335-7
更新日期:2016-04-01 00:00:00
abstract:INTRODUCTION:Cardiovascular dysfunction (CVD) is a well-recognized complication in patients with hyperthyroidism and is the major cause of mortality. Very few studies have compared the outcome of CVD following different treatment modalities. In this study we intended to compare treatment modalities (antithyroid drugs v...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-018-4571-4
更新日期:2018-05-01 00:00:00
abstract::Tamoxifen is being used successfully in breast cancer patients as adjuvant hormonal therapy. The aim of this retrospective cohort study is to evaluate the impact of tamoxifen on gallstone formation in postmenopausal breast cancer patients. A total of 3165 patients who were treated for invasive breast cancer between 19...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-002-6468-4
更新日期:2003-04-01 00:00:00
abstract:BACKGROUND:Despite its wide use, catheter tract recurrence after percutaneous biliary drainage (PBD) is rarely reported. However, one recent large-scale study reported a catheter tract recurrence rate as high as 5.2 % in patients with perihilar or distal bile duct cancer. We report on our 20 years of experience with ca...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-012-1844-1
更新日期:2013-02-01 00:00:00
abstract:BACKGROUND:Critical illness disproportionately affects people in low-income countries (LICs). Efforts to improve critical care in LICs must account for differences in demographics and infrastructure compared to high-income settings. Part of this effort includes the development and validation of intensive care unit (ICU...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-019-05078-9
更新日期:2019-10-01 00:00:00
abstract:BACKGROUND:The aim of this study was to assess the efficacy of the Nd:YAG laser for treating stage I squamous cell carcinoma (SCC) of the lip. The retrospective study was performed in a tertiary care referral hospital in northern India. METHODS:In January 2007 the records of patients treated by Nd:YAG laser ablation o...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-007-9363-1
更新日期:2008-04-01 00:00:00
abstract:BACKGROUND:Neuroendocrine neoplasia (NEN) with unknown primary site (NEN-CUP tumors) may have a poor prognosis. We evaluated the clinical presentation, therapy, outcome, and risk factors for adverse outcomes in patients who had these tumors. METHODS:In 243 patients who had NEN, a retrospective review was performed in ...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-015-2963-2
更新日期:2015-06-01 00:00:00
abstract:BACKGROUND:The fields of surgery and trauma care have largely been neglected in the global health discussion. As a result the idea that surgery is not safe or cost effective in resource-limited settings has gone unchallenged. The SIGN Online Surgical Database (SOSD) is now one of the largest databases on trauma surgery...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-012-1817-4
更新日期:2013-02-01 00:00:00